SrasV1, Main, Exploration, bibRecord, 003512

DNA hybridization biosensors using polylysine modified SPCEs

Identifieur interne : 003512 ( Main/Exploration ); précédent : 003511; suivant : 003513

DNA hybridization biosensors using polylysine modified SPCEs

Auteurs : Maria Diaz-Gonzalez [Espagne] ; Alfredo De La Escosura-Muniz [Espagne] ; Maria Begona Gonzalez-Garcia [Espagne] ; Agustin Costa-Garcia [Espagne]

Source :

Biosensors & bioelectronics [ 0956-5663 ] ; 2008.

RBID : Pascal:08-0340742

Descripteurs français

KwdFr :
- ADN (génétique), Carbone (), Hybridation d'acides nucléiques (), Polylysine (), Sondes oligonucléotidiques, Techniques de biocapteur (instrumentation), Virus du SRAS (isolement et purification), Électrodes.
MESH :
- génétique : ADN.
- isolement et purification : Virus du SRAS.
Pascal (Inist)
- Carbone, Hybridation d'acides nucléiques, Hybridation moléculaire, Biodétecteur, Alkaline phosphatase, Electrode carbone, Polylysine, Sondes oligonucléotidiques, Techniques de biocapteur, Virus syndrome respiratoire aigu sévère, Électrodes.

English descriptors

KwdEn :
- Alkaline phosphatase, Biosensing Techniques (instrumentation), Biosensor, Carbon (chemistry), Carbon electrode, DNA (genetics), Electrodes, Molecular hybridization, Nucleic Acid Hybridization (methods), Oligonucleotide Probes, Polylysine (chemistry), SARS Virus (isolation & purification), Severe acute respiratory syndrome virus.
MESH :
- chemical , chemistry : Carbon, Polylysine.
- chemical , genetics : DNA.
- instrumentation : Biosensing Techniques.
- isolation & purification : SARS Virus.
- methods : Nucleic Acid Hybridization.
- Electrodes, Oligonucleotide Probes.

Abstract

Two electrochemical DNA hybridization biosensors (genosensors) for the detection of a 30-mer sequence unique to severe acute respiratory syndrome (SARS) virus are described in this work. Both genosensors rely on the hybridization of the oligonucleotide target with its complementary probe, which is immobilized on positively charged polylysine modified screen-printed carbon electrodes (SPCEs), through electrostatic interactions. In one design, a biotinylated target is used and the detection of the hybridization reaction is monitored using alkaline phosphatase labeled streptavidin (S-AP). This enzyme catalyzes the hydrolysis of the substrate 3-indoxyl phosphate (3-IP) to indigo, which is then solubilized to indigo carmine and detected by means of cyclic voltammetry (CV). In the other design, the target is labeled using an Au(I) complex, sodium aurothiomalate, and the duplex formation is detected by measuring, for first time, the current generated by the hydrogen evolution catalyzed by the gold label. Using 30 min of hybridization time, a detection limit of 8 pM is calculated for the enzymatic genosensor. Although this good sensitivity cannot be reached with the metal label (0.5 nM), the use of this label allows a considerable decrease of the analysis time. Both genosensors do not require the modification of the oligonucleotide probe and using stringent experimental conditions (60min of hybridization time and 50% formamide in the hybridization buffer) can discriminate between a complementary oligonucleotide and an oligonucleotide with a three-base mismatch.

Affiliations:

Espagne

Le document en format XML

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<term>Carbon electrode</term>
<term>DNA (genetics)</term>
<term>Electrodes</term>
<term>Molecular hybridization</term>
<term>Nucleic Acid Hybridization (methods)</term>
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<term>Sondes oligonucléotidiques</term>
<term>Techniques de biocapteur (instrumentation)</term>
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<term>Oligonucleotide Probes</term>
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<term>Polylysine</term>
<term>Sondes oligonucléotidiques</term>
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<term>Virus syndrome respiratoire aigu sévère</term>
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<front><div type="abstract" xml:lang="en">Two electrochemical DNA hybridization biosensors (genosensors) for the detection of a 30-mer sequence unique to severe acute respiratory syndrome (SARS) virus are described in this work. Both genosensors rely on the hybridization of the oligonucleotide target with its complementary probe, which is immobilized on positively charged polylysine modified screen-printed carbon electrodes (SPCEs), through electrostatic interactions. In one design, a biotinylated target is used and the detection of the hybridization reaction is monitored using alkaline phosphatase labeled streptavidin (S-AP). This enzyme catalyzes the hydrolysis of the substrate 3-indoxyl phosphate (3-IP) to indigo, which is then solubilized to indigo carmine and detected by means of cyclic voltammetry (CV). In the other design, the target is labeled using an Au(I) complex, sodium aurothiomalate, and the duplex formation is detected by measuring, for first time, the current generated by the hydrogen evolution catalyzed by the gold label. Using 30 min of hybridization time, a detection limit of 8 pM is calculated for the enzymatic genosensor. Although this good sensitivity cannot be reached with the metal label (0.5 nM), the use of this label allows a considerable decrease of the analysis time. Both genosensors do not require the modification of the oligonucleotide probe and using stringent experimental conditions (60min of hybridization time and 50% formamide in the hybridization buffer) can discriminate between a complementary oligonucleotide and an oligonucleotide with a three-base mismatch.</div>
</front>
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<affiliations><list><country><li>Espagne</li>
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<tree><country name="Espagne"><noRegion><name sortKey="Diaz Gonzalez, Maria" sort="Diaz Gonzalez, Maria" uniqKey="Diaz Gonzalez M" first="Maria" last="Diaz-Gonzalez">Maria Diaz-Gonzalez</name>
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<name sortKey="Begona Gonzalez Garcia, Maria" sort="Begona Gonzalez Garcia, Maria" uniqKey="Begona Gonzalez Garcia M" first="Maria" last="Begona Gonzalez-Garcia">Maria Begona Gonzalez-Garcia</name>
<name sortKey="Costa Garcia, Agustin" sort="Costa Garcia, Agustin" uniqKey="Costa Garcia A" first="Agustin" last="Costa-Garcia">Agustin Costa-Garcia</name>
<name sortKey="De La Escosura Muniz, Alfredo" sort="De La Escosura Muniz, Alfredo" uniqKey="De La Escosura Muniz A" first="Alfredo" last="De La Escosura-Muniz">Alfredo De La Escosura-Muniz</name>
</country>
</tree>
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</record>

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DNA hybridization biosensors using polylysine modified SPCEs

DNA hybridization biosensors using polylysine modified SPCEs

Source :

Descripteurs français

English descriptors

Abstract

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