Severe acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 gene
Identifieur interne : 002F07 ( Main/Exploration ); précédent : 002F06; suivant : 002F08Severe acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 gene
Auteurs : Kam-Leung Siu [Hong Kong] ; Ching-Ping Chan [Hong Kong] ; Chris Chan [Hong Kong] ; Bo-Jian Zheng [Hong Kong] ; Dong-Yan Jin [Hong Kong]Source :
- Journal of general virology [ 0022-1317 ] ; 2009.
Descripteurs français
- KwdFr :
- Fibrinogène (génétique), Fibrinogène (métabolisme), Humains, Lignée cellulaire, Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Syndrome respiratoire aigu sévère (génétique), Syndrome respiratoire aigu sévère (métabolisme), Syndrome respiratoire aigu sévère (virologie), Transcription génétique, Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Fibrinogène, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- métabolisme : Fibrinogène, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
- Cell Line, Coronavirus, Fibrinogen (genetics), Fibrinogen (metabolism), Gene, Human, Humans, Microbiology, Nucleocapsid, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Protein, SARS Virus (genetics), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (genetics), Severe Acute Respiratory Syndrome (metabolism), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Transcription, Transcription, Genetic.
- MESH :
- chemical , genetics : Fibrinogen, Nucleocapsid Proteins.
- chemical , metabolism : Fibrinogen, Nucleocapsid Proteins.
- genetics : SARS Virus, Severe Acute Respiratory Syndrome.
- metabolism : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Cell Line, Humans, Transcription, Genetic.
Abstract
Among the structural and nonstructural proteins of severe acute respiratory syndrome coronavirus (SARS-CoV), the nucleocapsid (N) protein plays pivotal roles in the biology and pathogenesis of viral infection. N protein is thought to dysregulate cell signalling and the transcription of cellular genes, including FGL2, which encodes a prothrombinase implicated in vascular thrombosis, fibrin deposition and pneumocyte necrosis. Here, we showed that N protein expressed in cultured human cells was predominantly found in the cytoplasm and was competent in repressing the transcriptional activity driven by interferon-stimulated response elements. However, the expression of N protein did not influence the transcription from the FGL2 promoter. More importantly, N protein did not modulate the expression of FGL2 mRNA or protein in transfected or SARS-CoV-infected cells. Taken together, our findings did not support the model in which SARS-CoV N protein specifically modulates transcription of the FGL2 gene to cause fibrosis and vascular thrombosis.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000191
- to stream PascalFrancis, to step Curation: 000797
- to stream PascalFrancis, to step Checkpoint: 000184
- to stream Main, to step Merge: 002F61
- to stream PubMed, to step Corpus: 001907
- to stream PubMed, to step Curation: 001907
- to stream PubMed, to step Checkpoint: 001786
- to stream Ncbi, to step Merge: 001F10
- to stream Ncbi, to step Curation: 001F10
- to stream Ncbi, to step Checkpoint: 001F10
- to stream Main, to step Merge: 002A63
- to stream Main, to step Curation: 002F07
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Severe acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 gene</title>
<author><name sortKey="Siu, Kam Leung" sort="Siu, Kam Leung" uniqKey="Siu K" first="Kam-Leung" last="Siu">Kam-Leung Siu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, Ching Ping" sort="Chan, Ching Ping" uniqKey="Chan C" first="Ching-Ping" last="Chan">Ching-Ping Chan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, Chris" sort="Chan, Chris" uniqKey="Chan C" first="Chris" last="Chan">Chris Chan</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">09-0375283</idno>
<date when="2009">2009</date>
<idno type="stanalyst">PASCAL 09-0375283 INIST</idno>
<idno type="RBID">Pascal:09-0375283</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000191</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000797</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000184</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000184</idno>
<idno type="wicri:doubleKey">0022-1317:2009:Siu K:severe:acute:respiratory</idno>
<idno type="wicri:Area/Main/Merge">002F61</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:19423547</idno>
<idno type="wicri:Area/PubMed/Corpus">001907</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001907</idno>
<idno type="wicri:Area/PubMed/Curation">001907</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001907</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001786</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001786</idno>
<idno type="wicri:Area/Ncbi/Merge">001F10</idno>
<idno type="wicri:Area/Ncbi/Curation">001F10</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001F10</idno>
<idno type="wicri:doubleKey">0022-1317:2009:Siu K:severe:acute:respiratory</idno>
<idno type="wicri:Area/Main/Merge">002A63</idno>
<idno type="wicri:Area/Main/Curation">002F07</idno>
<idno type="wicri:Area/Main/Exploration">002F07</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Severe acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 gene</title>
<author><name sortKey="Siu, Kam Leung" sort="Siu, Kam Leung" uniqKey="Siu K" first="Kam-Leung" last="Siu">Kam-Leung Siu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, Ching Ping" sort="Chan, Ching Ping" uniqKey="Chan C" first="Ching-Ping" last="Chan">Ching-Ping Chan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, Chris" sort="Chan, Chris" uniqKey="Chan C" first="Chris" last="Chan">Chris Chan</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Biochemistry, Faculty of Medicine, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of general virology</title>
<title level="j" type="abbreviated">J. gen. virol.</title>
<idno type="ISSN">0022-1317</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of general virology</title>
<title level="j" type="abbreviated">J. gen. virol.</title>
<idno type="ISSN">0022-1317</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Line</term>
<term>Coronavirus</term>
<term>Fibrinogen (genetics)</term>
<term>Fibrinogen (metabolism)</term>
<term>Gene</term>
<term>Human</term>
<term>Humans</term>
<term>Microbiology</term>
<term>Nucleocapsid</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>Protein</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
<term>Severe Acute Respiratory Syndrome (genetics)</term>
<term>Severe Acute Respiratory Syndrome (metabolism)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Transcription</term>
<term>Transcription, Genetic</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Fibrinogène (génétique)</term>
<term>Fibrinogène (métabolisme)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Syndrome respiratoire aigu sévère (génétique)</term>
<term>Syndrome respiratoire aigu sévère (métabolisme)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Transcription génétique</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fibrinogen</term>
<term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fibrinogen</term>
<term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fibrinogène</term>
<term>Protéines nucléocapside</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fibrinogène</term>
<term>Protéines nucléocapside</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cell Line</term>
<term>Humans</term>
<term>Transcription, Genetic</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Homme</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Nucléocapside</term>
<term>Protéine</term>
<term>Transcription</term>
<term>Gène</term>
<term>Microbiologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Transcription génétique</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Among the structural and nonstructural proteins of severe acute respiratory syndrome coronavirus (SARS-CoV), the nucleocapsid (N) protein plays pivotal roles in the biology and pathogenesis of viral infection. N protein is thought to dysregulate cell signalling and the transcription of cellular genes, including FGL2, which encodes a prothrombinase implicated in vascular thrombosis, fibrin deposition and pneumocyte necrosis. Here, we showed that N protein expressed in cultured human cells was predominantly found in the cytoplasm and was competent in repressing the transcriptional activity driven by interferon-stimulated response elements. However, the expression of N protein did not influence the transcription from the FGL2 promoter. More importantly, N protein did not modulate the expression of FGL2 mRNA or protein in transfected or SARS-CoV-infected cells. Taken together, our findings did not support the model in which SARS-CoV N protein specifically modulates transcription of the FGL2 gene to cause fibrosis and vascular thrombosis.</div>
</front>
</TEI>
<affiliations><list><country><li>Hong Kong</li>
</country>
</list>
<tree><country name="Hong Kong"><noRegion><name sortKey="Siu, Kam Leung" sort="Siu, Kam Leung" uniqKey="Siu K" first="Kam-Leung" last="Siu">Kam-Leung Siu</name>
</noRegion>
<name sortKey="Chan, Ching Ping" sort="Chan, Ching Ping" uniqKey="Chan C" first="Ching-Ping" last="Chan">Ching-Ping Chan</name>
<name sortKey="Chan, Chris" sort="Chan, Chris" uniqKey="Chan C" first="Chris" last="Chan">Chris Chan</name>
<name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name>
<name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002F07 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002F07 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Exploration |type= RBID |clé= Pascal:09-0375283 |texte= Severe acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 gene }}
This area was generated with Dilib version V0.6.33. |