Airborne influenza virus detection with four aerosol samplers using molecular and infectivity assays: considerations for a new infectious virus aerosol sampler
Identifieur interne : 002E71 ( Main/Exploration ); précédent : 002E70; suivant : 002E72Airborne influenza virus detection with four aerosol samplers using molecular and infectivity assays: considerations for a new infectious virus aerosol sampler
Auteurs : P. Fabian [États-Unis] ; J. J. Mcdevitt [États-Unis] ; E. A. Houseman [États-Unis] ; D. K. Milton [États-Unis]Source :
- Indoor Air [ 0905-6947 ] ; 2009-10.
Abstract
Abstract Abstract As a first step in conducting studies of airborne influenza transmission, we compared the collection performance of an SKC Biosampler, a compact cascade impactor (CCI), Teflon filters, and gelatin filters by collecting aerosolized influenza virus in a one‐pass aerosol chamber. Influenza virus infectivity was determined using a fluorescent focus assay and influenza virus nucleic acid (originating from viable and non‐viable viruses) was measured using quantitative PCR. The results showed that the SKC Biosampler recovered and preserved influenza virus infectivity much better than the other samplers – the CCI, Teflon, and gelatin filters recovered only 7–22% of infectious viruses compared with the Biosampler. Total virus collection was not significantly different among the SKC Biosampler, the gelatin, and Teflon filters, but was significantly lower in the CCI. Results from this study show that a new sampler is needed for virus aerosol sampling, as commercially available samplers do not efficiently collect and conserve virus infectivity. Applications for a new sampler include studies of airborne disease transmission and bioterrorism monitoring. Design parameters for a new sampler include high collection efficiency for fine particles and liquid sampling media to preserve infectivity.
Url:
DOI: 10.1111/j.1600-0668.2009.00609.x
Affiliations:
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Milton</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health, Harvard School of Public Health, Boston, MA</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Work Environment Department, University of Massachusetts, Lowell, MA</wicri:regionArea><placeName><region type="state">Massachusetts</region><settlement type="city">Amherst (Massachusetts)</settlement></placeName><orgName type="university">Université du Massachusetts</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Indoor Air</title><title level="j" type="alt">INDOOR AIR</title><idno type="ISSN">0905-6947</idno><idno type="eISSN">1600-0668</idno><imprint><biblScope unit="vol">19</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="433">433</biblScope><biblScope unit="page" to="441">441</biblScope><biblScope unit="page-count">9</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2009-10">2009-10</date></imprint><idno type="ISSN">0905-6947</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0905-6947</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract Abstract As a first step in conducting studies of airborne influenza transmission, we compared the collection performance of an SKC Biosampler, a compact cascade impactor (CCI), Teflon filters, and gelatin filters by collecting aerosolized influenza virus in a one‐pass aerosol chamber. Influenza virus infectivity was determined using a fluorescent focus assay and influenza virus nucleic acid (originating from viable and non‐viable viruses) was measured using quantitative PCR. The results showed that the SKC Biosampler recovered and preserved influenza virus infectivity much better than the other samplers – the CCI, Teflon, and gelatin filters recovered only 7–22% of infectious viruses compared with the Biosampler. Total virus collection was not significantly different among the SKC Biosampler, the gelatin, and Teflon filters, but was significantly lower in the CCI. Results from this study show that a new sampler is needed for virus aerosol sampling, as commercially available samplers do not efficiently collect and conserve virus infectivity. Applications for a new sampler include studies of airborne disease transmission and bioterrorism monitoring. Design parameters for a new sampler include high collection efficiency for fine particles and liquid sampling media to preserve infectivity.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region><settlement><li>Amherst (Massachusetts)</li></settlement><orgName><li>Université du Massachusetts</li></orgName></list><tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Fabian, P" sort="Fabian, P" uniqKey="Fabian P" first="P." last="Fabian">P. Fabian</name></region><name sortKey="Fabian, P" sort="Fabian, P" uniqKey="Fabian P" first="P." last="Fabian">P. Fabian</name><name sortKey="Houseman, E A" sort="Houseman, E A" uniqKey="Houseman E" first="E. A." last="Houseman">E. A. Houseman</name><name sortKey="Mcdevitt, J J" sort="Mcdevitt, J J" uniqKey="Mcdevitt J" first="J. J." last="Mcdevitt">J. J. Mcdevitt</name><name sortKey="Mcdevitt, J J" sort="Mcdevitt, J J" uniqKey="Mcdevitt J" first="J. J." last="Mcdevitt">J. J. Mcdevitt</name><name sortKey="Milton, D K" sort="Milton, D K" uniqKey="Milton D" first="D. K." last="Milton">D. K. Milton</name><name sortKey="Milton, D K" sort="Milton, D K" uniqKey="Milton D" first="D. K." last="Milton">D. K. Milton</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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