Development of a consensus microarray method for identification of some highly pathogenic viruses
Identifieur interne : 002E26 ( Main/Exploration ); précédent : 002E25; suivant : 002E27Development of a consensus microarray method for identification of some highly pathogenic viruses
Auteurs : Kang Xiao-Ping [République populaire de Chine] ; Li Yong-Qiang [République populaire de Chine] ; Sun Qing-Ge [République populaire de Chine] ; Liu Hong [République populaire de Chine] ; Zhu Qing-Yu [République populaire de Chine] ; Yang Yin-Hui [République populaire de Chine]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2009-11.
Descripteurs français
- KwdFr :
- Analyse sur microréseau (), Animaux, Chine, Encéphale (virologie), Humains, Maladies des porcs (virologie), Maladies virales (diagnostic), Maladies virales (médecine vétérinaire), Sondes oligonucléotidiques (génétique), Souris, Sous-type H5N1 du virus de la grippe A (isolement et purification), Suidae, Techniques de laboratoire clinique (), Virus de l'encéphalite japonaise (espèce) (isolement et purification), Virus de l'encéphalite à tiques (sous-groupe), Virus de la dengue (isolement et purification), Virus de la fièvre jaune (isolement et purification), Virus du chikungunya (isolement et purification).
- MESH :
- diagnostic : Maladies virales.
- génétique : Sondes oligonucléotidiques.
- isolement et purification : Sous-type H5N1 du virus de la grippe A, Virus de l'encéphalite japonaise (espèce), Virus de la dengue, Virus de la fièvre jaune, Virus du chikungunya.
- médecine vétérinaire : Maladies virales.
- virologie : Encéphale, Maladies des porcs.
- Analyse sur microréseau, Animaux, Chine, Humains, Souris, Suidae, Techniques de laboratoire clinique, Virus de l'encéphalite à tiques (sous-groupe).
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Animals, Brain (virology), Chikungunya virus (isolation & purification), China, Clinical Laboratory Techniques (methods), Dengue Virus (isolation & purification), Encephalitis Virus, Japanese (isolation & purification), Encephalitis Viruses, Tick-Borne, Humans, Influenza A Virus, H5N1 Subtype (isolation & purification), Mice, Microarray Analysis (methods), Oligonucleotide Probes (genetics), Swine, Swine Diseases (virology), Virus Diseases (diagnosis), Virus Diseases (veterinary), Yellow fever virus (isolation & purification).
- MESH :
- chemical , genetics : Oligonucleotide Probes.
- geographic : China.
- diagnosis : Virus Diseases.
- isolation & purification : Chikungunya virus, Dengue Virus, Encephalitis Virus, Japanese, Influenza A Virus, H5N1 Subtype, Yellow fever virus.
- methods : Clinical Laboratory Techniques, Microarray Analysis.
- veterinary : Virus Diseases.
- virology : Brain, Swine Diseases.
- Teeft :
- Animals, Assay, Avian virus, Cdna, Cdna synthesis, Chik, Chikungunya virus, Clin, Clin microbiol, Dengue virus, Encephalitis, Encephalitis Viruses, Tick-Borne, Genome, Genus, Genus level, Genus probes, Hanta, Hanta virus, Host cells, Host genome, Humans, Hybridization, Hybridization results, Japanese encephalitis virus, Mice, Microarray, Microbiol, Nucleic acids, Oligonucleotide, Oligonucleotide probes, Pathogen, Pathogenic viruses, Primer, Probe, Rna, Shanxi province, Species level, Species levels, Species probes, Swine, Transcription, Transcription primers, Unknown virus, Viral, Viral genome, Viral pathogens, Viral species, Virol, Virus, Virus genome, Yellow fever virus.
Abstract
Some highly pathogenic viruses, such as Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Hanta virus, SARS‐CoV, and H5N1 avian influenza virus can cause severe infectious diseases. However, the consensus method for detecting these viruses has not been well established. A rapid and sensitive microarray approach for detection of these viruses and a panel of specific probes covering nine genera and 16 virus species were designed. 70‐mer oligonucleotides were used at the genus level and 50‐mer oligonucleotides were at the species level, respectively. To decrease the interference of the host genome in hybridization, the consensus genus primers were designed and used to reverse transcribe only virus genome. The synthesis of the second strand was carried out with a random primer sequence (5′‐GTTTCCCAGTAGGTCTCNNNNNNNN‐3′). The amplified products were labeled and processed for microarray analyses. This microarray‐based method used the highly conserved consensus primers to synthesize specifically the virus cDNA and could identify effectively Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Tick borne encephalitis virus, and H5N1 avian influenza virus. Using this method, one unknown virus isolated from pig brain in Shanxi Province, China was identified. This method may have an important potential application for the diagnosis of virus infection. J. Med. Virol. 81:1945–1950, 2009. © 2009 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.21602
Affiliations:
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type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">81</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1945">1945</biblScope><biblScope unit="page" to="1950">1950</biblScope><biblScope unit="page-count">6</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2009-11">2009-11</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Brain (virology)</term><term>Chikungunya virus (isolation & purification)</term><term>China</term><term>Clinical Laboratory Techniques (methods)</term><term>Dengue Virus (isolation & 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purification)</term><term>Suidae</term><term>Techniques de laboratoire clinique ()</term><term>Virus de l'encéphalite japonaise (espèce) (isolement et purification)</term><term>Virus de l'encéphalite à tiques (sous-groupe)</term><term>Virus de la dengue (isolement et purification)</term><term>Virus de la fièvre jaune (isolement et purification)</term><term>Virus du chikungunya (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Oligonucleotide Probes</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sondes oligonucléotidiques</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Chikungunya virus</term><term>Dengue Virus</term><term>Encephalitis Virus, Japanese</term><term>Influenza A Virus, H5N1 Subtype</term><term>Yellow fever virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term><term>Virus de l'encéphalite japonaise (espèce)</term><term>Virus de la dengue</term><term>Virus de la fièvre jaune</term><term>Virus du chikungunya</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Clinical Laboratory Techniques</term><term>Microarray Analysis</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Encéphale</term><term>Maladies des porcs</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Brain</term><term>Swine Diseases</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Animals</term><term>Assay</term><term>Avian virus</term><term>Cdna</term><term>Cdna synthesis</term><term>Chik</term><term>Chikungunya virus</term><term>Clin</term><term>Clin microbiol</term><term>Dengue virus</term><term>Encephalitis</term><term>Encephalitis Viruses, Tick-Borne</term><term>Genome</term><term>Genus</term><term>Genus level</term><term>Genus probes</term><term>Hanta</term><term>Hanta virus</term><term>Host cells</term><term>Host genome</term><term>Humans</term><term>Hybridization</term><term>Hybridization results</term><term>Japanese encephalitis virus</term><term>Mice</term><term>Microarray</term><term>Microbiol</term><term>Nucleic acids</term><term>Oligonucleotide</term><term>Oligonucleotide probes</term><term>Pathogen</term><term>Pathogenic viruses</term><term>Primer</term><term>Probe</term><term>Rna</term><term>Shanxi province</term><term>Species level</term><term>Species levels</term><term>Species probes</term><term>Swine</term><term>Transcription</term><term>Transcription primers</term><term>Unknown virus</term><term>Viral</term><term>Viral genome</term><term>Viral pathogens</term><term>Viral species</term><term>Virol</term><term>Virus</term><term>Virus genome</term><term>Yellow fever virus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse sur microréseau</term><term>Animaux</term><term>Chine</term><term>Humains</term><term>Souris</term><term>Suidae</term><term>Techniques de laboratoire clinique</term><term>Virus de l'encéphalite à tiques (sous-groupe)</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Some highly pathogenic viruses, such as Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Hanta virus, SARS‐CoV, and H5N1 avian influenza virus can cause severe infectious diseases. However, the consensus method for detecting these viruses has not been well established. A rapid and sensitive microarray approach for detection of these viruses and a panel of specific probes covering nine genera and 16 virus species were designed. 70‐mer oligonucleotides were used at the genus level and 50‐mer oligonucleotides were at the species level, respectively. To decrease the interference of the host genome in hybridization, the consensus genus primers were designed and used to reverse transcribe only virus genome. The synthesis of the second strand was carried out with a random primer sequence (5′‐GTTTCCCAGTAGGTCTCNNNNNNNN‐3′). The amplified products were labeled and processed for microarray analyses. This microarray‐based method used the highly conserved consensus primers to synthesize specifically the virus cDNA and could identify effectively Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Tick borne encephalitis virus, and H5N1 avian influenza virus. Using this method, one unknown virus isolated from pig brain in Shanxi Province, China was identified. This method may have an important potential application for the diagnosis of virus infection. J. Med. Virol. 81:1945–1950, 2009. © 2009 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Xiao Ing, Kang" sort="Xiao Ing, Kang" uniqKey="Xiao Ing K" first="Kang" last="Xiao-Ping">Kang Xiao-Ping</name></noRegion><name sortKey="Hong, Liu" sort="Hong, Liu" uniqKey="Hong L" first="Liu" last="Hong">Liu Hong</name><name sortKey="Qing E, Sun" sort="Qing E, Sun" uniqKey="Qing E S" first="Sun" last="Qing-Ge">Sun Qing-Ge</name><name sortKey="Qing U, Zhu" sort="Qing U, Zhu" uniqKey="Qing U Z" first="Zhu" last="Qing-Yu">Zhu Qing-Yu</name><name sortKey="Yin Ui, Yang" sort="Yin Ui, Yang" uniqKey="Yin Ui Y" first="Yang" last="Yin-Hui">Yang Yin-Hui</name><name sortKey="Yin Ui, Yang" sort="Yin Ui, Yang" uniqKey="Yin Ui Y" first="Yang" last="Yin-Hui">Yang Yin-Hui</name><name sortKey="Yong Iang, Li" sort="Yong Iang, Li" uniqKey="Yong Iang L" first="Li" last="Yong-Qiang">Li Yong-Qiang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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