Analysis of putative recombination hot sites in the S gene of canine coronaviruses.
Identifieur interne : 002B86 ( Main/Exploration ); précédent : 002B85; suivant : 002B87Analysis of putative recombination hot sites in the S gene of canine coronaviruses.
Auteurs : Y Y Wang [République populaire de Chine] ; C P LuSource :
- Acta virologica [ 0001-723X ] ; 2009.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Analyse de séquence d'ADN, Animaux, Chats, Chiens, Clonage moléculaire, Coronavirus canin (génétique), Coronavirus canin (métabolisme), Coronavirus félin (génétique), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Humains, Protéines de l'enveloppe virale (génétique), Recombinaison génétique, Séquence nucléotidique, Virus de la gastroentérite transmissible (génétique).
- MESH :
- génétique : ARN viral, Coronavirus canin, Coronavirus félin, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus de la gastroentérite transmissible.
- métabolisme : Coronavirus canin.
- Analyse de séquence d'ADN, Animaux, Chats, Chiens, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Recombinaison génétique, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Cats, Cloning, Molecular, Coronavirus, Canine (genetics), Coronavirus, Canine (metabolism), Coronavirus, Feline (genetics), Dogs, Humans, Membrane Glycoproteins (genetics), Molecular Sequence Data, RNA, Viral (genetics), Recombination, Genetic, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus, Transmissible gastroenteritis virus (genetics), Viral Envelope Proteins (genetics).
- MESH :
- chemical , genetics : Membrane Glycoproteins, RNA, Viral, Viral Envelope Proteins.
- genetics : Coronavirus, Canine, Coronavirus, Feline, Transmissible gastroenteritis virus.
- metabolism : Coronavirus, Canine.
- Animals, Base Sequence, Cats, Cloning, Molecular, Dogs, Humans, Molecular Sequence Data, Recombination, Genetic, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus.
Abstract
The S gene sequence of Canine coronavirus strain 1-71 (CCoV 1-71) was cloned, sequenced, and compared to those of other CCoVs, Transmissible gastroenteritis virus (TGEV), and Feline coronavirus (FCoV). The sequence analysis showed that CCoV 1-71 displayed a 98.8-99.8% identity with CCoVs strains V1, K378, and GP. Four putative recombination sites were found at the 5'-end of the S gene, namely at nt 53, 75, 425, 991. Both sequences flanking each site were significantly different. Three recombination hot regions were found on the S gene, namely at nt 337-437, 1545-3405, and 4203-4356, which shared a common recombination signal with Group 2 coronaviruses. The G/CTAAAAA/GT sequence downstream of the recombination site may represent a specific recombination signal in CCoVs. The CCoV 1-71 S protein sequence was found to be similar to those of other CCoVs except for several N-glycosylation sites at the N-terminus of the S protein, which could be related to the differences in virulence and cell tropism in individual CCoVs. This study indicated that the similarity of CCoVs in virulence and tropism was mostly acquired by the homologous RNA recombination and not only by simple mutation and selection.
DOI: 10.4149/av_2009_02_111
PubMed: 19537912
Affiliations:
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sortKey="Wang, Y Y" sort="Wang, Y Y" uniqKey="Wang Y" first="Y Y" last="Wang">Y Y Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Lu, C P" sort="Lu, C P" uniqKey="Lu C" first="C P" last="Lu">C P Lu</name></author></analytic><series><title level="j">Acta virologica</title><idno type="ISSN">0001-723X</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cats</term><term>Cloning, Molecular</term><term>Coronavirus, Canine (genetics)</term><term>Coronavirus, Canine (metabolism)</term><term>Coronavirus, Feline (genetics)</term><term>Dogs</term><term>Humans</term><term>Membrane Glycoproteins (genetics)</term><term>Molecular Sequence Data</term><term>RNA, Viral (genetics)</term><term>Recombination, Genetic</term><term>Sequence Analysis, DNA</term><term>Spike Glycoprotein, Coronavirus</term><term>Transmissible gastroenteritis virus (genetics)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Chats</term><term>Chiens</term><term>Clonage moléculaire</term><term>Coronavirus canin (génétique)</term><term>Coronavirus canin (métabolisme)</term><term>Coronavirus félin (génétique)</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Humains</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Recombinaison génétique</term><term>Séquence nucléotidique</term><term>Virus de la gastroentérite transmissible (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>RNA, Viral</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus, Canine</term><term>Coronavirus, Feline</term><term>Transmissible gastroenteritis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Coronavirus canin</term><term>Coronavirus félin</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Virus de la gastroentérite transmissible</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coronavirus, Canine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Coronavirus canin</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cats</term><term>Cloning, Molecular</term><term>Dogs</term><term>Humans</term><term>Molecular Sequence Data</term><term>Recombination, Genetic</term><term>Sequence Analysis, DNA</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Chats</term><term>Chiens</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Recombinaison génétique</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The S gene sequence of Canine coronavirus strain 1-71 (CCoV 1-71) was cloned, sequenced, and compared to those of other CCoVs, Transmissible gastroenteritis virus (TGEV), and Feline coronavirus (FCoV). The sequence analysis showed that CCoV 1-71 displayed a 98.8-99.8% identity with CCoVs strains V1, K378, and GP. Four putative recombination sites were found at the 5'-end of the S gene, namely at nt 53, 75, 425, 991. Both sequences flanking each site were significantly different. Three recombination hot regions were found on the S gene, namely at nt 337-437, 1545-3405, and 4203-4356, which shared a common recombination signal with Group 2 coronaviruses. The G/CTAAAAA/GT sequence downstream of the recombination site may represent a specific recombination signal in CCoVs. The CCoV 1-71 S protein sequence was found to be similar to those of other CCoVs except for several N-glycosylation sites at the N-terminus of the S protein, which could be related to the differences in virulence and cell tropism in individual CCoVs. This study indicated that the similarity of CCoVs in virulence and tropism was mostly acquired by the homologous RNA recombination and not only by simple mutation and selection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Lu, C P" sort="Lu, C P" uniqKey="Lu C" first="C P" last="Lu">C P Lu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Y Y" sort="Wang, Y Y" uniqKey="Wang Y" first="Y Y" last="Wang">Y Y Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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