Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV
Identifieur interne : 002B00 ( Main/Exploration ); précédent : 002A99; suivant : 002B01Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV
Auteurs : Yanbo Lv ; Zhihua Ruan ; Li Wang ; Bing Ni ; Yuzhang WuSource :
- BMC Immunology [ 1471-2172 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Antigène HLA-A2, Antigènes HLA-A (génétique), Antigènes HLA-A (immunologie), Antigènes HLA-A (métabolisme), Cartographie épitopique, Coronavirus (immunologie), Cytotoxicité immunologique, Déterminants antigéniques des lymphocytes T (génétique), Déterminants antigéniques des lymphocytes T (immunologie), Déterminants antigéniques des lymphocytes T (métabolisme), Fragments peptidiques (génétique), Fragments peptidiques (immunologie), Fragments peptidiques (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Immunisation, Interféron gamma (métabolisme), Liaison aux protéines, Lignée cellulaire, Lymphocytes T cytotoxiques (anatomopathologie), Lymphocytes T cytotoxiques (immunologie), Lymphocytes T cytotoxiques (métabolisme), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Souris, Souris transgéniques, Syndrome respiratoire aigu sévère (immunologie), Séquence conservée.
- MESH :
- anatomopathologie : Lymphocytes T cytotoxiques.
- génétique : Antigènes HLA-A, Déterminants antigéniques des lymphocytes T, Fragments peptidiques, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Antigènes HLA-A, Coronavirus, Déterminants antigéniques des lymphocytes T, Fragments peptidiques, Glycoprotéines membranaires, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère.
- métabolisme : Antigènes HLA-A, Déterminants antigéniques des lymphocytes T, Fragments peptidiques, Glycoprotéines membranaires, Interféron gamma, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale.
- Animaux, Antigène HLA-A2, Cartographie épitopique, Cytotoxicité immunologique, Glycoprotéine de spicule des coronavirus, Humains, Immunisation, Liaison aux protéines, Lignée cellulaire, Souris, Souris transgéniques, Séquence conservée.
English descriptors
- KwdEn :
- Animals, Cell Line, Conserved Sequence, Coronavirus (immunology), Cytotoxicity, Immunologic, Epitope Mapping, Epitopes, T-Lymphocyte (genetics), Epitopes, T-Lymphocyte (immunology), Epitopes, T-Lymphocyte (metabolism), HLA-A Antigens (genetics), HLA-A Antigens (immunology), HLA-A Antigens (metabolism), HLA-A2 Antigen, Humans, Immunization, Interferon-gamma (metabolism), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Mice, Transgenic, Peptide Fragments (genetics), Peptide Fragments (immunology), Peptide Fragments (metabolism), Protein Binding, Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, T-Lymphocytes, Cytotoxic (immunology), T-Lymphocytes, Cytotoxic (metabolism), T-Lymphocytes, Cytotoxic (pathology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , genetics : Epitopes, T-Lymphocyte, HLA-A Antigens, Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins.
- immunology : Coronavirus, Epitopes, T-Lymphocyte, HLA-A Antigens, Membrane Glycoproteins, Peptide Fragments, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic, Viral Envelope Proteins.
- chemical , metabolism : Epitopes, T-Lymphocyte, HLA-A Antigens, Interferon-gamma, Membrane Glycoproteins, Peptide Fragments, T-Lymphocytes, Cytotoxic, Viral Envelope Proteins.
- pathology : T-Lymphocytes, Cytotoxic.
- Animals, Cell Line, Conserved Sequence, Cytotoxicity, Immunologic, Epitope Mapping, HLA-A2 Antigen, Humans, Immunization, Mice, Mice, Transgenic, Protein Binding, Spike Glycoprotein, Coronavirus.
Abstract
The spike (S) protein is a major structural glycoprotein of coronavirus (CoV), the causal agent of severe acute respiratory syndrome (SARS). The S protein is a potent target for SARS-specific cell-mediated immune responses. However, the mechanism CoV pathogenesis in SARS and the role of special CTLs in virus clearance are still largely uncharacterized. Here, we describe a study that leads to the identification of a novel HLA-A*0201-restricted epitope from conserved regions of S protein.
First, different SARS-CoV sequences were analyzed to predict eight candidate peptides from conserved regions of the S protein based upon HLA-A*0201 binding and proteosomal cleavage. Four of eight candidate peptides were tested by HLA-A*0201 binding assays. Among the four candidate peptides, Sp8 (S958-966, VLNDILSRL) induced specific CTLs both
These results suggest that Sp8 is a naturally processed epitope. We propose that Sp8 epitope should help in the characterization of mechanisms of virus control and immunopathology in SARS-CoV infection.
The online version of this article (doi:10.1186/1471-2172-10-61) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2172-10-61
PubMed: 19958537
PubMed Central: 2792222
Affiliations:
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Le document en format XML
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id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Ruan, Zhihua" sort="Ruan, Zhihua" uniqKey="Ruan Z" first="Zhihua" last="Ruan">Zhihua Ruan</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Li" sort="Wang, Li" uniqKey="Wang L" first="Li" last="Wang">Li Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Ni, Bing" sort="Ni, Bing" uniqKey="Ni B" first="Bing" last="Ni">Bing Ni</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Yuzhang" sort="Wu, Yuzhang" uniqKey="Wu Y" first="Yuzhang" last="Wu">Yuzhang Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">BMC Immunology</title><idno type="eISSN">1471-2172</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" 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Coronavirus</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>T-Lymphocytes, Cytotoxic (metabolism)</term><term>T-Lymphocytes, Cytotoxic (pathology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigène HLA-A2</term><term>Antigènes HLA-A (génétique)</term><term>Antigènes HLA-A (immunologie)</term><term>Antigènes HLA-A (métabolisme)</term><term>Cartographie épitopique</term><term>Coronavirus (immunologie)</term><term>Cytotoxicité immunologique</term><term>Déterminants antigéniques des lymphocytes T (génétique)</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Déterminants antigéniques des lymphocytes T (métabolisme)</term><term>Fragments peptidiques (génétique)</term><term>Fragments peptidiques (immunologie)</term><term>Fragments peptidiques 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xml:lang="en"><term>Coronavirus</term><term>Epitopes, T-Lymphocyte</term><term>HLA-A Antigens</term><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes, Cytotoxic</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>HLA-A Antigens</term><term>Interferon-gamma</term><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>T-Lymphocytes, Cytotoxic</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes HLA-A</term><term>Déterminants antigéniques des lymphocytes T</term><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Interféron gamma</term><term>Lymphocytes T cytotoxiques</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Conserved Sequence</term><term>Cytotoxicity, Immunologic</term><term>Epitope Mapping</term><term>HLA-A2 Antigen</term><term>Humans</term><term>Immunization</term><term>Mice</term><term>Mice, Transgenic</term><term>Protein Binding</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigène HLA-A2</term><term>Cartographie épitopique</term><term>Cytotoxicité immunologique</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Immunisation</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Souris</term><term>Souris transgéniques</term><term>Séquence conservée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The spike (S) protein is a major structural glycoprotein of coronavirus (CoV), the causal agent of severe acute respiratory syndrome (SARS). The S protein is a potent target for SARS-specific cell-mediated immune responses. However, the mechanism CoV pathogenesis in SARS and the role of special CTLs in virus clearance are still largely uncharacterized. Here, we describe a study that leads to the identification of a novel HLA-A*0201-restricted epitope from conserved regions of S protein.</p></sec><sec><title>Results</title><p>First, different SARS-CoV sequences were analyzed to predict eight candidate peptides from conserved regions of the S protein based upon HLA-A*0201 binding and proteosomal cleavage. Four of eight candidate peptides were tested by HLA-A*0201 binding assays. Among the four candidate peptides, Sp8 (S<sub>958-966</sub>, VLNDILSRL) induced specific CTLs both <italic>ex vivo</italic> in PBLs of healthy HLA-A2<sup>+</sup> donors and in HLA-A2.1/K<sup>b</sup> transgenic mice immunized with a plasmid encoding full-length S protein. The immunized mice released IFN-γ and lysed target cells upon stimulation with Sp8 peptide-pulsed autologous dendritic cells in comparison to other candidates.</p></sec><sec><title>Conclusion</title><p>These results suggest that Sp8 is a naturally processed epitope. We propose that Sp8 epitope should help in the characterization of mechanisms of virus control and immunopathology in SARS-CoV infection.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2172-10-61) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name></author><author><name sortKey="Werf, S Van Der" uniqKey="Werf S">S van der Werf</name></author><author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S Becker</name></author><author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H Rabenau</name></author><author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name></author><author><name sortKey="Kolesnikova, L" uniqKey="Kolesnikova L">L Kolesnikova</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D Erdman</name></author><author><name sortKey="Goldsmith, Cs" uniqKey="Goldsmith C">CS Goldsmith</name></author><author><name sortKey="Zaki, Sr" uniqKey="Zaki S">SR Zaki</name></author><author><name sortKey="Peret, T" uniqKey="Peret T">T Peret</name></author><author><name sortKey="Emery, S" uniqKey="Emery S">S Emery</name></author><author><name sortKey="Tong, S" uniqKey="Tong S">S Tong</name></author><author><name sortKey="Urbani, C" uniqKey="Urbani C">C Urbani</name></author><author><name sortKey="Comer, Ja" uniqKey="Comer J">JA Comer</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author><author><name sortKey="Lai, St" uniqKey="Lai S">ST Lai</name></author><author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Yam, Ly" uniqKey="Yam L">LY Yam</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author><author><name sortKey="Nicholls, J" uniqKey="Nicholls J">J Nicholls</name></author><author><name sortKey="Yee, Wk" uniqKey="Yee W">WK Yee</name></author><author><name sortKey="Yan, Ww" uniqKey="Yan W">WW Yan</name></author><author><name sortKey="Cheung, Mt" uniqKey="Cheung M">MT Cheung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="He, Yq" uniqKey="He Y">YQ He</name></author><author><name sortKey="Liu, Xl" uniqKey="Liu X">XL Liu</name></author><author><name sortKey="Zhuang, Zx" uniqKey="Zhuang Z">ZX Zhuang</name></author><author><name sortKey="Cheung, Cl" uniqKey="Cheung C">CL Cheung</name></author><author><name sortKey="Luo, Sw" uniqKey="Luo S">SW Luo</name></author><author><name sortKey="Li, Ph" uniqKey="Li P">PH Li</name></author><author><name sortKey="Zhang, Lj" uniqKey="Zhang L">LJ Zhang</name></author><author><name sortKey="Guan, Yj" uniqKey="Guan Y">YJ Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Skowronski, Dm" uniqKey="Skowronski D">DM Skowronski</name></author><author><name sortKey="Astell, C" uniqKey="Astell C">C Astell</name></author><author><name sortKey="Brunham, Rc" uniqKey="Brunham R">RC Brunham</name></author><author><name sortKey="Low, De" uniqKey="Low D">DE Low</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author><author><name sortKey="Roper, Rl" uniqKey="Roper R">RL Roper</name></author><author><name sortKey="Talbot, Pj" uniqKey="Talbot P">PJ Talbot</name></author><author><name sortKey="Tam, T" uniqKey="Tam T">T Tam</name></author><author><name sortKey="Babiuk, L" uniqKey="Babiuk L">L Babiuk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Normile, D" uniqKey="Normile D">D Normile</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Orellana, C" uniqKey="Orellana C">C Orellana</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paterson, R" uniqKey="Paterson R">R Paterson</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author><author><name sortKey="Gao, X" uniqKey="Gao X">X Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhong, X" uniqKey="Zhong X">X Zhong</name></author><author><name sortKey="Yang, H" uniqKey="Yang H">H Yang</name></author><author><name sortKey="Guo, Zf" uniqKey="Guo Z">ZF Guo</name></author><author><name sortKey="Sin, Wy" uniqKey="Sin W">WY Sin</name></author><author><name sortKey="Chen, W" uniqKey="Chen W">W Chen</name></author><author><name sortKey="Xu, J" uniqKey="Xu J">J Xu</name></author><author><name sortKey="Fu, L" uniqKey="Fu L">L Fu</name></author><author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name></author><author><name sortKey="Mak, Ck" uniqKey="Mak C">CK Mak</name></author><author><name sortKey="Cheng, Cs" uniqKey="Cheng C">CS Cheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, T" uniqKey="Li T">T Li</name></author><author><name sortKey="Xie, J" uniqKey="Xie J">J Xie</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Fan, H" uniqKey="Fan H">H Fan</name></author><author><name sortKey="Baril, L" uniqKey="Baril L">L Baril</name></author><author><name sortKey="Qiu, Z" uniqKey="Qiu Z">Z Qiu</name></author><author><name sortKey="Han, Y" uniqKey="Han Y">Y Han</name></author><author><name sortKey="Xu, W" uniqKey="Xu W">W Xu</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="You, H" uniqKey="You H">H You</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dutta, Nk" uniqKey="Dutta N">NK Dutta</name></author><author><name sortKey="Mazumdar, K" uniqKey="Mazumdar K">K Mazumdar</name></author><author><name sortKey="Lee, Bh" uniqKey="Lee B">BH Lee</name></author><author><name sortKey="Baek, Mw" uniqKey="Baek M">MW Baek</name></author><author><name sortKey="Kim, Dj" uniqKey="Kim D">DJ Kim</name></author><author><name sortKey="Na, Yr" uniqKey="Na Y">YR Na</name></author><author><name sortKey="Park, Sh" uniqKey="Park S">SH Park</name></author><author><name sortKey="Lee, Hk" uniqKey="Lee H">HK Lee</name></author><author><name sortKey="Kariwa, H" uniqKey="Kariwa H">H Kariwa</name></author><author><name sortKey="Mai Le, Q" uniqKey="Mai Le Q">Q Mai le</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, H" uniqKey="Jin H">H Jin</name></author><author><name sortKey="Xiao, C" uniqKey="Xiao C">C Xiao</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name></author><author><name sortKey="Kang, Y" uniqKey="Kang Y">Y Kang</name></author><author><name sortKey="Ma, Y" uniqKey="Ma Y">Y Ma</name></author><author><name sortKey="Zhu, K" uniqKey="Zhu K">K Zhu</name></author><author><name sortKey="Xie, Q" uniqKey="Xie Q">Q Xie</name></author><author><name sortKey="Tu, Y" uniqKey="Tu Y">Y Tu</name></author><author><name sortKey="Yu, Y" uniqKey="Yu Y">Y Yu</name></author><author><name sortKey="Wang, B" uniqKey="Wang B">B Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="See, Rh" uniqKey="See R">RH See</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author><author><name sortKey="Lawrence, Dj" uniqKey="Lawrence D">DJ Lawrence</name></author><author><name sortKey="Mok, Cp" uniqKey="Mok C">CP Mok</name></author><author><name sortKey="Rowe, T" uniqKey="Rowe T">T Rowe</name></author><author><name sortKey="Zitzow, La" uniqKey="Zitzow L">LA Zitzow</name></author><author><name sortKey="Karunakaran, Kp" uniqKey="Karunakaran K">KP Karunakaran</name></author><author><name sortKey="Voss, Tg" uniqKey="Voss T">TG Voss</name></author><author><name sortKey="Brunham, Rc" uniqKey="Brunham R">RC Brunham</name></author><author><name sortKey="Gauldie, J" uniqKey="Gauldie J">J Gauldie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buchholz, Uj" uniqKey="Buchholz U">UJ Buchholz</name></author><author><name sortKey="Bukreyev, A" uniqKey="Bukreyev A">A Bukreyev</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L Yang</name></author><author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name></author><author><name sortKey="Murphy, Br" uniqKey="Murphy B">BR Murphy</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author><author><name sortKey="Collins, Pl" uniqKey="Collins P">PL Collins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zakhartchouk, An" uniqKey="Zakhartchouk A">AN Zakhartchouk</name></author><author><name sortKey="Sharon, C" uniqKey="Sharon C">C Sharon</name></author><author><name sortKey="Satkunarajah, M" uniqKey="Satkunarajah M">M Satkunarajah</name></author><author><name sortKey="Auperin, T" uniqKey="Auperin T">T Auperin</name></author><author><name sortKey="Viswanathan, S" uniqKey="Viswanathan S">S Viswanathan</name></author><author><name sortKey="Mutwiri, G" uniqKey="Mutwiri G">G Mutwiri</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author><author><name sortKey="See, Rh" uniqKey="See R">RH See</name></author><author><name sortKey="Brunham, Rc" uniqKey="Brunham R">RC Brunham</name></author><author><name sortKey="Finlay, Bb" uniqKey="Finlay B">BB Finlay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhi, Y" uniqKey="Zhi Y">Y Zhi</name></author><author><name sortKey="Kobinger, Gp" uniqKey="Kobinger G">GP Kobinger</name></author><author><name sortKey="Jordan, H" uniqKey="Jordan H">H Jordan</name></author><author><name sortKey="Suchma, K" uniqKey="Suchma K">K Suchma</name></author><author><name sortKey="Weiss, Sr" uniqKey="Weiss S">SR Weiss</name></author><author><name sortKey="Shen, H" uniqKey="Shen H">H Shen</name></author><author><name sortKey="Schumer, G" uniqKey="Schumer G">G Schumer</name></author><author><name sortKey="Gao, G" uniqKey="Gao G">G Gao</name></author><author><name sortKey="Boyer, Jl" uniqKey="Boyer J">JL Boyer</name></author><author><name sortKey="Crystal, Rg" uniqKey="Crystal R">RG Crystal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Cy" uniqKey="Zhang C">CY Zhang</name></author><author><name sortKey="Wei, Jf" uniqKey="Wei J">JF Wei</name></author><author><name sortKey="He, Sh" uniqKey="He S">SH He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, X" uniqKey="Tang X">X Tang</name></author><author><name sortKey="Yin, C" uniqKey="Yin C">C Yin</name></author><author><name sortKey="Zhang, F" uniqKey="Zhang F">F Zhang</name></author><author><name sortKey="Fu, Y" uniqKey="Fu Y">Y Fu</name></author><author><name sortKey="Chen, W" uniqKey="Chen W">W Chen</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author><author><name sortKey="Jia, W" uniqKey="Jia W">W Jia</name></author><author><name sortKey="Xu, A" uniqKey="Xu A">A Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name></author><author><name sortKey="Grey, Hm" uniqKey="Grey H">HM Grey</name></author><author><name sortKey="Kubo, Rt" uniqKey="Kubo R">RT Kubo</name></author><author><name sortKey="Sette, A" uniqKey="Sette A">A Sette</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author><author><name sortKey="Hou, J" uniqKey="Hou J">J Hou</name></author><author><name sortKey="Jiang, X" uniqKey="Jiang X">X Jiang</name></author><author><name sortKey="Ma, S" uniqKey="Ma S">S Ma</name></author><author><name sortKey="Meng, M" uniqKey="Meng M">M Meng</name></author><author><name sortKey="Wang, B" uniqKey="Wang B">B Wang</name></author><author><name sortKey="Zhang, M" uniqKey="Zhang M">M Zhang</name></author><author><name sortKey="Zhang, M" uniqKey="Zhang M">M Zhang</name></author><author><name sortKey="Tang, X" uniqKey="Tang X">X Tang</name></author><author><name sortKey="Zhang, F" uniqKey="Zhang F">F Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsao, Yp" uniqKey="Tsao Y">YP Tsao</name></author><author><name sortKey="Lin, Jy" uniqKey="Lin J">JY Lin</name></author><author><name sortKey="Jan, Jt" uniqKey="Jan J">JT Jan</name></author><author><name sortKey="Leng, Ch" uniqKey="Leng C">CH Leng</name></author><author><name sortKey="Chu, Cc" uniqKey="Chu C">CC Chu</name></author><author><name sortKey="Yang, Yc" uniqKey="Yang Y">YC Yang</name></author><author><name sortKey="Chen, Sl" uniqKey="Chen S">SL Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Yd" uniqKey="Wang Y">YD Wang</name></author><author><name sortKey="Chen, Wf" uniqKey="Chen W">WF Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, M" uniqKey="Zhou M">M Zhou</name></author><author><name sortKey="Xu, D" uniqKey="Xu D">D Xu</name></author><author><name sortKey="Li, X" uniqKey="Li X">X Li</name></author><author><name sortKey="Li, H" uniqKey="Li H">H Li</name></author><author><name sortKey="Shan, M" uniqKey="Shan M">M Shan</name></author><author><name sortKey="Tang, J" uniqKey="Tang J">J Tang</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Wang, Fs" uniqKey="Wang F">FS Wang</name></author><author><name sortKey="Zhu, X" uniqKey="Zhu X">X Zhu</name></author><author><name sortKey="Tao, H" uniqKey="Tao H">H Tao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, J" uniqKey="Huang J">J Huang</name></author><author><name sortKey="Cao, Y" uniqKey="Cao Y">Y Cao</name></author><author><name sortKey="Du, J" uniqKey="Du J">J Du</name></author><author><name sortKey="Bu, X" uniqKey="Bu X">X Bu</name></author><author><name sortKey="Ma, R" uniqKey="Ma R">R Ma</name></author><author><name sortKey="Wu, C" uniqKey="Wu C">C Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Passoni, L" uniqKey="Passoni L">L Passoni</name></author><author><name sortKey="Scardino, A" uniqKey="Scardino A">A Scardino</name></author><author><name sortKey="Bertazzoli, C" uniqKey="Bertazzoli C">C Bertazzoli</name></author><author><name sortKey="Gallo, B" uniqKey="Gallo B">B Gallo</name></author><author><name sortKey="Coluccia, Am" uniqKey="Coluccia A">AM Coluccia</name></author><author><name sortKey="Lemonnier, Fa" uniqKey="Lemonnier F">FA Lemonnier</name></author><author><name sortKey="Kosmatopoulos, K" uniqKey="Kosmatopoulos K">K Kosmatopoulos</name></author><author><name sortKey="Gambacorti Passerini, C" uniqKey="Gambacorti Passerini C">C Gambacorti-Passerini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salter, Rd" uniqKey="Salter R">RD Salter</name></author><author><name sortKey="Cresswell, P" uniqKey="Cresswell P">P Cresswell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nicholls, Jm" uniqKey="Nicholls J">JM Nicholls</name></author><author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name></author><author><name sortKey="Lee, Kc" uniqKey="Lee K">KC Lee</name></author><author><name sortKey="Ng, Wf" uniqKey="Ng W">WF Ng</name></author><author><name sortKey="Lai, St" uniqKey="Lai S">ST Lai</name></author><author><name sortKey="Leung, Cy" uniqKey="Leung C">CY Leung</name></author><author><name sortKey="Chu, Cm" uniqKey="Chu C">CM Chu</name></author><author><name sortKey="Hui, Pk" uniqKey="Hui P">PK Hui</name></author><author><name sortKey="Mak, Kl" uniqKey="Mak K">KL Mak</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Singh, H" uniqKey="Singh H">H Singh</name></author><author><name sortKey="Raghava, Gp" uniqKey="Raghava G">GP Raghava</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rammensee, H" uniqKey="Rammensee H">H Rammensee</name></author><author><name sortKey="Bachmann, J" uniqKey="Bachmann J">J Bachmann</name></author><author><name sortKey="Emmerich, Np" uniqKey="Emmerich N">NP Emmerich</name></author><author><name sortKey="Bachor, Oa" uniqKey="Bachor O">OA Bachor</name></author><author><name sortKey="Stevanovic, S" uniqKey="Stevanovic S">S Stevanovic</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Irwin, Mj" uniqKey="Irwin M">MJ Irwin</name></author><author><name sortKey="Heath, Wr" uniqKey="Heath W">WR Heath</name></author><author><name sortKey="Sherman, La" uniqKey="Sherman L">LA Sherman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cao, X" uniqKey="Cao X">X Cao</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="He, L" uniqKey="He L">L He</name></author><author><name sortKey="Xie, Z" uniqKey="Xie Z">Z Xie</name></author><author><name sortKey="Ma, S" uniqKey="Ma S">S Ma</name></author><author><name sortKey="Tao, Q" uniqKey="Tao Q">Q Tao</name></author><author><name sortKey="Yu, Y" uniqKey="Yu Y">Y Yu</name></author><author><name sortKey="Hamada, H" uniqKey="Hamada H">H Hamada</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Talmor, M" uniqKey="Talmor M">M Talmor</name></author><author><name sortKey="Mirza, A" uniqKey="Mirza A">A Mirza</name></author><author><name sortKey="Turley, S" uniqKey="Turley S">S Turley</name></author><author><name sortKey="Mellman, I" uniqKey="Mellman I">I Mellman</name></author><author><name sortKey="Hoffman, La" uniqKey="Hoffman L">LA Hoffman</name></author><author><name sortKey="Steinman, Rm" uniqKey="Steinman R">RM Steinman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiang, R" uniqKey="Xiang R">R Xiang</name></author><author><name sortKey="Lode, Hn" uniqKey="Lode H">HN Lode</name></author><author><name sortKey="Dreier, T" uniqKey="Dreier T">T Dreier</name></author><author><name sortKey="Gillies, Sd" uniqKey="Gillies S">SD Gillies</name></author><author><name sortKey="Reisfeld, Ra" uniqKey="Reisfeld R">RA Reisfeld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mccutcheon, M" uniqKey="Mccutcheon M">M McCutcheon</name></author><author><name sortKey="Wehner, N" uniqKey="Wehner N">N Wehner</name></author><author><name sortKey="Wensky, A" uniqKey="Wensky A">A Wensky</name></author><author><name sortKey="Kushner, M" uniqKey="Kushner M">M Kushner</name></author><author><name sortKey="Doan, S" uniqKey="Doan S">S Doan</name></author><author><name sortKey="Hsiao, L" uniqKey="Hsiao L">L Hsiao</name></author><author><name sortKey="Calabresi, P" uniqKey="Calabresi P">P Calabresi</name></author><author><name sortKey="Ha, T" uniqKey="Ha T">T Ha</name></author><author><name sortKey="Tran, Tv" uniqKey="Tran T">TV Tran</name></author><author><name sortKey="Tate, Km" uniqKey="Tate K">KM Tate</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Lv, Yanbo" sort="Lv, Yanbo" uniqKey="Lv Y" first="Yanbo" last="Lv">Yanbo Lv</name><name sortKey="Ni, Bing" sort="Ni, Bing" uniqKey="Ni B" first="Bing" last="Ni">Bing Ni</name><name sortKey="Ruan, Zhihua" sort="Ruan, Zhihua" uniqKey="Ruan Z" first="Zhihua" last="Ruan">Zhihua Ruan</name><name sortKey="Wang, Li" sort="Wang, Li" uniqKey="Wang L" first="Li" last="Wang">Li Wang</name><name sortKey="Wu, Yuzhang" sort="Wu, Yuzhang" uniqKey="Wu Y" first="Yuzhang" last="Wu">Yuzhang Wu</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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