Elastase-mediated activation of the severe acute respiratory syndrome coronavirus spike protein at discrete sites within the S2 domain.
Identifieur interne : 002547 ( Main/Exploration ); précédent : 002546; suivant : 002548Elastase-mediated activation of the severe acute respiratory syndrome coronavirus spike protein at discrete sites within the S2 domain.
Auteurs : Sandrine Belouzard [États-Unis] ; Ikenna Madu ; Gary R. WhittakerSource :
- The Journal of biological chemistry [ 1083-351X ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Bovins, Cellules Vero, Fusion cellulaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Mutagenèse, Mutation, Pancreatic elastase (métabolisme), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Sites de fixation, Structure tertiaire des protéines, Séquence d'acides aminés, Trypsine (métabolisme), Virus du SRAS (métabolisme), Virus du SRAS (physiologie).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- métabolisme : Glycoprotéines membranaires, Pancreatic elastase, Protéines de l'enveloppe virale, Trypsine, Virus du SRAS.
- physiologie : Virus du SRAS.
- Animaux, Bovins, Cellules Vero, Fusion cellulaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Mutagenèse, Mutation, Protéines de l'enveloppe virale, Sites de fixation, Structure tertiaire des protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Binding Sites, Cattle, Cell Fusion, Chlorocebus aethiops, Humans, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Mutagenesis, Mutation, Pancreatic Elastase (metabolism), Protein Structure, Tertiary, SARS Virus (metabolism), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Trypsin (metabolism), Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Membrane Glycoproteins, Pancreatic Elastase, Trypsin, Viral Envelope Proteins.
- metabolism : SARS Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Animals, Binding Sites, Cattle, Cell Fusion, Chlorocebus aethiops, Humans, Mutagenesis, Mutation, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
Proteolytic priming is a common method of controlling the activation of membrane fusion mediated by viral glycoproteins. The severe acute respiratory syndrome coronavirus spike protein (SARS-CoV S) can be primed by a variety of host cell proteases, with proteolytic cleavage occurring both as the S1/S2 boundary and adjacent to a fusion peptide in the S2 domain. Here, we studied the priming of SARS-CoV S by elastase and show an important role for residue Thr(795) in the S2 domain. A series of alanine mutants were generated in the vicinity of the S2 cleavage site, with the goal of examining elastase-mediated cleavage within S2. Both proteolytic cleavage and fusion activation were modulated by altering the cleavage site position. We propose a novel mechanism whereby SARS-CoV fusion protein function can be controlled by spatial regulation of the proteolytic priming site, with important implications for viral pathogenesis.
DOI: 10.1074/jbc.M110.103275
PubMed: 20507992
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001689
- to stream PubMed, to step Curation: 001689
- to stream PubMed, to step Checkpoint: 001682
- to stream Ncbi, to step Merge: 002147
- to stream Ncbi, to step Curation: 002147
- to stream Ncbi, to step Checkpoint: 002147
- to stream Main, to step Merge: 002584
- to stream Main, to step Curation: 002547
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Elastase-mediated activation of the severe acute respiratory syndrome coronavirus spike protein at discrete sites within the S2 domain.</title><author><name sortKey="Belouzard, Sandrine" sort="Belouzard, Sandrine" uniqKey="Belouzard S" first="Sandrine" last="Belouzard">Sandrine Belouzard</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853</wicri:regionArea><orgName type="university">Université Cornell</orgName><placeName><settlement type="city">Ithaca (New York)</settlement><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Madu, Ikenna" sort="Madu, Ikenna" uniqKey="Madu I" first="Ikenna" last="Madu">Ikenna Madu</name></author><author><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R" last="Whittaker">Gary R. Whittaker</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20507992</idno><idno type="pmid">20507992</idno><idno type="doi">10.1074/jbc.M110.103275</idno><idno type="wicri:Area/PubMed/Corpus">001689</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001689</idno><idno type="wicri:Area/PubMed/Curation">001689</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001689</idno><idno type="wicri:Area/PubMed/Checkpoint">001682</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001682</idno><idno type="wicri:Area/Ncbi/Merge">002147</idno><idno type="wicri:Area/Ncbi/Curation">002147</idno><idno type="wicri:Area/Ncbi/Checkpoint">002147</idno><idno type="wicri:Area/Main/Merge">002584</idno><idno type="wicri:Area/Main/Curation">002547</idno><idno type="wicri:Area/Main/Exploration">002547</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Elastase-mediated activation of the severe acute respiratory syndrome coronavirus spike protein at discrete sites within the S2 domain.</title><author><name sortKey="Belouzard, Sandrine" sort="Belouzard, Sandrine" uniqKey="Belouzard S" first="Sandrine" last="Belouzard">Sandrine Belouzard</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853</wicri:regionArea><orgName type="university">Université Cornell</orgName><placeName><settlement type="city">Ithaca (New York)</settlement><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Madu, Ikenna" sort="Madu, Ikenna" uniqKey="Madu I" first="Ikenna" last="Madu">Ikenna Madu</name></author><author><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R" last="Whittaker">Gary R. Whittaker</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="eISSN">1083-351X</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Binding Sites</term><term>Cattle</term><term>Cell Fusion</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mutagenesis</term><term>Mutation</term><term>Pancreatic Elastase (metabolism)</term><term>Protein Structure, Tertiary</term><term>SARS Virus (metabolism)</term><term>SARS Virus (physiology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Trypsin (metabolism)</term><term>Vero Cells</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Cellules Vero</term><term>Fusion cellulaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Mutagenèse</term><term>Mutation</term><term>Pancreatic elastase (métabolisme)</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Sites de fixation</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Trypsine (métabolisme)</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Pancreatic Elastase</term><term>Trypsin</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Pancreatic elastase</term><term>Protéines de l'enveloppe virale</term><term>Trypsine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Binding Sites</term><term>Cattle</term><term>Cell Fusion</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Mutagenesis</term><term>Mutation</term><term>Protein Structure, Tertiary</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Cellules Vero</term><term>Fusion cellulaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Mutagenèse</term><term>Mutation</term><term>Protéines de l'enveloppe virale</term><term>Sites de fixation</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Proteolytic priming is a common method of controlling the activation of membrane fusion mediated by viral glycoproteins. The severe acute respiratory syndrome coronavirus spike protein (SARS-CoV S) can be primed by a variety of host cell proteases, with proteolytic cleavage occurring both as the S1/S2 boundary and adjacent to a fusion peptide in the S2 domain. Here, we studied the priming of SARS-CoV S by elastase and show an important role for residue Thr(795) in the S2 domain. A series of alanine mutants were generated in the vicinity of the S2 cleavage site, with the goal of examining elastase-mediated cleavage within S2. Both proteolytic cleavage and fusion activation were modulated by altering the cleavage site position. We propose a novel mechanism whereby SARS-CoV fusion protein function can be controlled by spatial regulation of the proteolytic priming site, with important implications for viral pathogenesis.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><noCountry><name sortKey="Madu, Ikenna" sort="Madu, Ikenna" uniqKey="Madu I" first="Ikenna" last="Madu">Ikenna Madu</name><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R" last="Whittaker">Gary R. Whittaker</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Belouzard, Sandrine" sort="Belouzard, Sandrine" uniqKey="Belouzard S" first="Sandrine" last="Belouzard">Sandrine Belouzard</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002547 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002547 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20507992
|texte= Elastase-mediated activation of the severe acute respiratory syndrome coronavirus spike protein at discrete sites within the S2 domain.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20507992" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |