Importance of controlling nanotube density for highly sensitive and reliable biosensors functional in physiological conditions.
Identifieur interne : 002520 ( Main/Exploration ); précédent : 002519; suivant : 002521Importance of controlling nanotube density for highly sensitive and reliable biosensors functional in physiological conditions.
Auteurs : Fumiaki N. Ishikawa [États-Unis] ; Marco Curreli ; C Anders Olson ; Hsiang-I Liao ; Ren Sun ; Richard W. Roberts ; Richard J. Cote ; Mark E. Thompson ; Chongwu ZhouSource :
- ACS nano [ 1936-086X ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Bovins, Conductivité électrique, Conformation des protéines, Limite de détection, Marqueurs biologiques (), Marqueurs biologiques (métabolisme), Modèles moléculaires, Nanotechnologie (), Nanotubes de carbone (), Protéines nucléocapside (), Protéines nucléocapside (métabolisme), Reproductibilité des résultats, Streptavidine (métabolisme), Techniques de biocapteur (), Techniques de biocapteur (instrumentation), Transistors électroniques, Virus du SRAS.
- MESH :
- métabolisme : Marqueurs biologiques, Protéines nucléocapside, Streptavidine, Techniques de biocapteur.
- Animaux, Bovins, Conductivité électrique, Conformation des protéines, Limite de détection, Marqueurs biologiques, Modèles moléculaires, Nanotechnologie, Nanotubes de carbone, Protéines nucléocapside, Reproductibilité des résultats, Techniques de biocapteur, Transistors électroniques, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Biomarkers (chemistry), Biomarkers (metabolism), Biosensing Techniques (instrumentation), Biosensing Techniques (methods), Cattle, Electric Conductivity, Limit of Detection, Models, Molecular, Nanotechnology (methods), Nanotubes, Carbon (chemistry), Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (metabolism), Protein Conformation, Reproducibility of Results, SARS Virus, Streptavidin (metabolism), Transistors, Electronic.
- MESH :
- chemical , chemistry : Biomarkers, Nanotubes, Carbon, Nucleocapsid Proteins.
- chemical , metabolism : Biomarkers, Nucleocapsid Proteins, Streptavidin.
- instrumentation : Biosensing Techniques.
- methods : Biosensing Techniques, Nanotechnology.
- Animals, Cattle, Electric Conductivity, Limit of Detection, Models, Molecular, Protein Conformation, Reproducibility of Results, SARS Virus, Transistors, Electronic.
Abstract
Biosensors utilizing carbon nanotube field-effect transistors have a tremendous potential to serve as the basis for the next generation of diagnostic systems. While nanotubes have been employed in the fabrication of multiple sensors, little attention has previously been paid to how the nanotube density affects the biosensor performance. We conducted a systematic study of the effect of density on the performance of nanotube biosensors and discovered that this parameter is crucial to achieving consistently high performance. We found that devices with lower density offer higher sensitivity in terms of both detection limit and magnitude of response. The low density nanotube devices resulted in a detection limit of 1 pM in an electrolyte buffer containing high levels of electrolytes (ionic concentration ∼140 mM, matching the ionic strength of serum and plasma). Further investigation suggested that the enhanced sensitivity arises from the semiconductor-like behavior-strong gate dependence and lower capacitance-of the nanotube network at low density. Finally, we used the density-optimized nanotube biosensors to detect the nucleocapsid (N) protein of the SARS virus and demonstrated improved detection limits under physiological conditions. Our results show that it is critical to carefully tune the nanotube density in order to fabricate sensitive and reliable devices.
DOI: 10.1021/nn101198u
PubMed: 21028792
Affiliations:
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Ishikawa</name><affiliation wicri:level="4"><nlm:affiliation>Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Curreli, Marco" sort="Curreli, Marco" uniqKey="Curreli M" first="Marco" last="Curreli">Marco Curreli</name></author><author><name sortKey="Olson, C Anders" sort="Olson, C Anders" uniqKey="Olson C" first="C Anders" last="Olson">C Anders Olson</name></author><author><name sortKey="Liao, Hsiang I" sort="Liao, Hsiang I" uniqKey="Liao H" first="Hsiang-I" last="Liao">Hsiang-I Liao</name></author><author><name sortKey="Sun, Ren" sort="Sun, Ren" uniqKey="Sun R" first="Ren" last="Sun">Ren Sun</name></author><author><name sortKey="Roberts, Richard W" sort="Roberts, Richard W" uniqKey="Roberts R" first="Richard W" last="Roberts">Richard W. 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Ishikawa</name><affiliation wicri:level="4"><nlm:affiliation>Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Curreli, Marco" sort="Curreli, Marco" uniqKey="Curreli M" first="Marco" last="Curreli">Marco Curreli</name></author><author><name sortKey="Olson, C Anders" sort="Olson, C Anders" uniqKey="Olson C" first="C Anders" last="Olson">C Anders Olson</name></author><author><name sortKey="Liao, Hsiang I" sort="Liao, Hsiang I" uniqKey="Liao H" first="Hsiang-I" last="Liao">Hsiang-I Liao</name></author><author><name sortKey="Sun, Ren" sort="Sun, Ren" uniqKey="Sun R" first="Ren" last="Sun">Ren Sun</name></author><author><name sortKey="Roberts, Richard W" sort="Roberts, Richard W" uniqKey="Roberts R" first="Richard W" last="Roberts">Richard W. Roberts</name></author><author><name sortKey="Cote, Richard J" sort="Cote, Richard J" uniqKey="Cote R" first="Richard J" last="Cote">Richard J. Cote</name></author><author><name sortKey="Thompson, Mark E" sort="Thompson, Mark E" uniqKey="Thompson M" first="Mark E" last="Thompson">Mark E. Thompson</name></author><author><name sortKey="Zhou, Chongwu" sort="Zhou, Chongwu" uniqKey="Zhou C" first="Chongwu" last="Zhou">Chongwu Zhou</name></author></analytic><series><title level="j">ACS nano</title><idno type="eISSN">1936-086X</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biomarkers (chemistry)</term><term>Biomarkers (metabolism)</term><term>Biosensing Techniques (instrumentation)</term><term>Biosensing Techniques (methods)</term><term>Cattle</term><term>Electric Conductivity</term><term>Limit of Detection</term><term>Models, Molecular</term><term>Nanotechnology (methods)</term><term>Nanotubes, Carbon (chemistry)</term><term>Nucleocapsid Proteins (chemistry)</term><term>Nucleocapsid Proteins (metabolism)</term><term>Protein Conformation</term><term>Reproducibility of Results</term><term>SARS Virus</term><term>Streptavidin (metabolism)</term><term>Transistors, Electronic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Conductivité électrique</term><term>Conformation des protéines</term><term>Limite de détection</term><term>Marqueurs biologiques ()</term><term>Marqueurs biologiques (métabolisme)</term><term>Modèles moléculaires</term><term>Nanotechnologie ()</term><term>Nanotubes de carbone ()</term><term>Protéines nucléocapside ()</term><term>Protéines nucléocapside (métabolisme)</term><term>Reproductibilité des résultats</term><term>Streptavidine (métabolisme)</term><term>Techniques de biocapteur ()</term><term>Techniques de biocapteur (instrumentation)</term><term>Transistors électroniques</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Biomarkers</term><term>Nanotubes, Carbon</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biomarkers</term><term>Nucleocapsid Proteins</term><term>Streptavidin</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Nanotechnology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Marqueurs biologiques</term><term>Protéines nucléocapside</term><term>Streptavidine</term><term>Techniques de biocapteur</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Electric Conductivity</term><term>Limit of Detection</term><term>Models, Molecular</term><term>Protein Conformation</term><term>Reproducibility of Results</term><term>SARS Virus</term><term>Transistors, Electronic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Conductivité électrique</term><term>Conformation des protéines</term><term>Limite de détection</term><term>Marqueurs biologiques</term><term>Modèles moléculaires</term><term>Nanotechnologie</term><term>Nanotubes de carbone</term><term>Protéines nucléocapside</term><term>Reproductibilité des résultats</term><term>Techniques de biocapteur</term><term>Transistors électroniques</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Biosensors utilizing carbon nanotube field-effect transistors have a tremendous potential to serve as the basis for the next generation of diagnostic systems. While nanotubes have been employed in the fabrication of multiple sensors, little attention has previously been paid to how the nanotube density affects the biosensor performance. We conducted a systematic study of the effect of density on the performance of nanotube biosensors and discovered that this parameter is crucial to achieving consistently high performance. We found that devices with lower density offer higher sensitivity in terms of both detection limit and magnitude of response. The low density nanotube devices resulted in a detection limit of 1 pM in an electrolyte buffer containing high levels of electrolytes (ionic concentration ∼140 mM, matching the ionic strength of serum and plasma). Further investigation suggested that the enhanced sensitivity arises from the semiconductor-like behavior-strong gate dependence and lower capacitance-of the nanotube network at low density. Finally, we used the density-optimized nanotube biosensors to detect the nucleocapsid (N) protein of the SARS virus and demonstrated improved detection limits under physiological conditions. Our results show that it is critical to carefully tune the nanotube density in order to fabricate sensitive and reliable devices.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region><settlement><li>Los Angeles</li></settlement><orgName><li>Université de Californie du Sud</li></orgName></list><tree><noCountry><name sortKey="Cote, Richard J" sort="Cote, Richard J" uniqKey="Cote R" first="Richard J" last="Cote">Richard J. Cote</name><name sortKey="Curreli, Marco" sort="Curreli, Marco" uniqKey="Curreli M" first="Marco" last="Curreli">Marco Curreli</name><name sortKey="Liao, Hsiang I" sort="Liao, Hsiang I" uniqKey="Liao H" first="Hsiang-I" last="Liao">Hsiang-I Liao</name><name sortKey="Olson, C Anders" sort="Olson, C Anders" uniqKey="Olson C" first="C Anders" last="Olson">C Anders Olson</name><name sortKey="Roberts, Richard W" sort="Roberts, Richard W" uniqKey="Roberts R" first="Richard W" last="Roberts">Richard W. Roberts</name><name sortKey="Sun, Ren" sort="Sun, Ren" uniqKey="Sun R" first="Ren" last="Sun">Ren Sun</name><name sortKey="Thompson, Mark E" sort="Thompson, Mark E" uniqKey="Thompson M" first="Mark E" last="Thompson">Mark E. Thompson</name><name sortKey="Zhou, Chongwu" sort="Zhou, Chongwu" uniqKey="Zhou C" first="Chongwu" last="Zhou">Chongwu Zhou</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Ishikawa, Fumiaki N" sort="Ishikawa, Fumiaki N" uniqKey="Ishikawa F" first="Fumiaki N" last="Ishikawa">Fumiaki N. Ishikawa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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