Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion
Identifieur interne : 002227 ( Main/Exploration ); précédent : 002226; suivant : 002228Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion
Auteurs : Jung-Eun Park [Corée du Sud] ; Deu John M. Cruz [Corée du Sud] ; Hyun-Jin Shin [Corée du Sud]Source :
- Archives of Virology [ 0304-8608 ] ; 2011-10-01.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Fusion membranaire (), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Infections à coronavirus (métabolisme), Infections à coronavirus (physiopathologie), Infections à coronavirus (virologie), Maturation post-traductionnelle des protéines (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (métabolisme), Trypsine (pharmacologie), Virus de la diarrhée porcine épidémique (), Virus de la diarrhée porcine épidémique (génétique), Virus de la diarrhée porcine épidémique (métabolisme).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus de la diarrhée porcine épidémique.
- métabolisme : Glycoprotéines membranaires, Infections à coronavirus, Protéines de l'enveloppe virale, Récepteurs viraux, Virus de la diarrhée porcine épidémique.
- pharmacologie : Trypsine.
- physiopathologie : Infections à coronavirus.
- virologie : Infections à coronavirus.
- Animaux, Cellules Vero, Fusion membranaire, Glycoprotéine de spicule des coronavirus, Maturation post-traductionnelle des protéines, Virus de la diarrhée porcine épidémique.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Coronavirus Infections (metabolism), Coronavirus Infections (physiopathology), Coronavirus Infections (virology), Membrane Fusion (drug effects), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Porcine epidemic diarrhea virus (drug effects), Porcine epidemic diarrhea virus (genetics), Porcine epidemic diarrhea virus (metabolism), Protein Processing, Post-Translational (drug effects), Receptors, Virus (metabolism), Spike Glycoprotein, Coronavirus, Trypsin (pharmacology), Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- drug effects : Membrane Fusion, Porcine epidemic diarrhea virus, Protein Processing, Post-Translational.
- genetics : Porcine epidemic diarrhea virus.
- metabolism : Coronavirus Infections, Membrane Glycoproteins, Porcine epidemic diarrhea virus, Receptors, Virus, Viral Envelope Proteins.
- chemical , pharmacology : Trypsin.
- physiopathology : Coronavirus Infections.
- virology : Coronavirus Infections.
- Animals, Chlorocebus aethiops, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
Abstract: Porcine epidemic diarrhea virus (PEDV) infection in Vero cells is facilitated by trypsin through an undefined mechanism. The present study describes the mode of action of trypsin in enhancing PEDV infection in Vero cells during different stage of the virus life cycle. During the viral entry stage, trypsin increased the penetration of Vero-cell-attached PEDV by approximately twofold. However, trypsin treatment of viruses before receptor binding did not enhance infectivity, indicating that receptor binding is essentially required for trypsin-mediated entry upon PEDV infection. Trypsin treatment during the budding stage of virus infection induces an obvious cytopathic effect in infected cells. Furthermore, we also show that the PEDV spike (S) glycoprotein is cleaved by trypsin in virions that are bound to the receptor, but not in free virions. These findings indicate that trypsin affects only cell-attached PEDV and increases infectivity and syncytium formation in PEDV-infected Vero cells by cleavage of the PEDV S protein. These findings strongly suggest that the PEDV S protein may undergo a conformational change after receptor binding and cleavage by exogenous trypsin, which induces membrane fusion.
Url:
DOI: 10.1007/s00705-011-1044-6
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000621
- to stream Istex, to step Curation: 000621
- to stream Istex, to step Checkpoint: 000725
- to stream PubMed, to step Corpus: 001503
- to stream PubMed, to step Curation: 001503
- to stream PubMed, to step Checkpoint: 001446
- to stream Ncbi, to step Merge: 002348
- to stream Ncbi, to step Curation: 002348
- to stream Ncbi, to step Checkpoint: 002348
- to stream Main, to step Merge: 002255
- to stream Main, to step Curation: 002227
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion</title><author><name sortKey="Park, Jung Eun" sort="Park, Jung Eun" uniqKey="Park J" first="Jung-Eun" last="Park">Jung-Eun Park</name></author><author><name sortKey="Cruz, Deu John M" sort="Cruz, Deu John M" uniqKey="Cruz D" first="Deu John M." last="Cruz">Deu John M. Cruz</name></author><author><name sortKey="Shin, Hyun Jin" sort="Shin, Hyun Jin" uniqKey="Shin H" first="Hyun-Jin" last="Shin">Hyun-Jin Shin</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F18470B606E38211523ADB3C0CC942C98ED6E6CD</idno><date when="2011" year="2011">2011</date><idno type="doi">10.1007/s00705-011-1044-6</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-8RC7ZBQ9-X/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000621</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000621</idno><idno type="wicri:Area/Istex/Curation">000621</idno><idno type="wicri:Area/Istex/Checkpoint">000725</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000725</idno><idno type="wicri:doubleKey">0304-8608:2011:Park J:receptor:bound:porcine</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:21667287</idno><idno type="wicri:Area/PubMed/Corpus">001503</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001503</idno><idno type="wicri:Area/PubMed/Curation">001503</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001503</idno><idno type="wicri:Area/PubMed/Checkpoint">001446</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001446</idno><idno type="wicri:Area/Ncbi/Merge">002348</idno><idno type="wicri:Area/Ncbi/Curation">002348</idno><idno type="wicri:Area/Ncbi/Checkpoint">002348</idno><idno type="wicri:Area/Main/Merge">002255</idno><idno type="wicri:Area/Main/Curation">002227</idno><idno type="wicri:Area/Main/Exploration">002227</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion</title><author><name sortKey="Park, Jung Eun" sort="Park, Jung Eun" uniqKey="Park J" first="Jung-Eun" last="Park">Jung-Eun Park</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Infectious Diseases, #302, Animal Hospital, College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yuseong, 305-764, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation></author><author><name sortKey="Cruz, Deu John M" sort="Cruz, Deu John M" uniqKey="Cruz D" first="Deu John M." last="Cruz">Deu John M. Cruz</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Infectious Diseases, #302, Animal Hospital, College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yuseong, 305-764, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation></author><author><name sortKey="Shin, Hyun Jin" sort="Shin, Hyun Jin" uniqKey="Shin H" first="Hyun-Jin" last="Shin">Hyun-Jin Shin</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Infectious Diseases, #302, Animal Hospital, College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yuseong, 305-764, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Institute of Veterinary Medicine, Chungnam National University, Daejeon</wicri:regionArea><wicri:noRegion>Daejeon</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Corée du Sud</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="sub">Official Journal of the Virology Division of the International Union of Microbiological Societies</title><title level="j" type="abbrev">Arch Virol</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer Vienna</publisher><pubPlace>Vienna</pubPlace><date type="published" when="2011-10-01">2011-10-01</date><biblScope unit="volume">156</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="1749">1749</biblScope><biblScope unit="page" to="1756">1756</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (metabolism)</term><term>Coronavirus Infections (physiopathology)</term><term>Coronavirus Infections (virology)</term><term>Membrane Fusion (drug effects)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Porcine epidemic diarrhea virus (drug effects)</term><term>Porcine epidemic diarrhea virus (genetics)</term><term>Porcine epidemic diarrhea virus (metabolism)</term><term>Protein Processing, Post-Translational (drug effects)</term><term>Receptors, Virus (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Trypsin (pharmacology)</term><term>Vero Cells</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Fusion membranaire ()</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Infections à coronavirus (métabolisme)</term><term>Infections à coronavirus (physiopathologie)</term><term>Infections à coronavirus (virologie)</term><term>Maturation post-traductionnelle des protéines ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Récepteurs viraux (métabolisme)</term><term>Trypsine (pharmacologie)</term><term>Virus de la diarrhée porcine épidémique ()</term><term>Virus de la diarrhée porcine épidémique (génétique)</term><term>Virus de la diarrhée porcine épidémique (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Membrane Fusion</term><term>Porcine epidemic diarrhea virus</term><term>Protein Processing, Post-Translational</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Porcine epidemic diarrhea virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Virus de la diarrhée porcine épidémique</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coronavirus Infections</term><term>Membrane Glycoproteins</term><term>Porcine epidemic diarrhea virus</term><term>Receptors, Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Infections à coronavirus</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term><term>Virus de la diarrhée porcine épidémique</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Trypsine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Trypsin</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Fusion membranaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Maturation post-traductionnelle des protéines</term><term>Virus de la diarrhée porcine épidémique</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Porcine epidemic diarrhea virus (PEDV) infection in Vero cells is facilitated by trypsin through an undefined mechanism. The present study describes the mode of action of trypsin in enhancing PEDV infection in Vero cells during different stage of the virus life cycle. During the viral entry stage, trypsin increased the penetration of Vero-cell-attached PEDV by approximately twofold. However, trypsin treatment of viruses before receptor binding did not enhance infectivity, indicating that receptor binding is essentially required for trypsin-mediated entry upon PEDV infection. Trypsin treatment during the budding stage of virus infection induces an obvious cytopathic effect in infected cells. Furthermore, we also show that the PEDV spike (S) glycoprotein is cleaved by trypsin in virions that are bound to the receptor, but not in free virions. These findings indicate that trypsin affects only cell-attached PEDV and increases infectivity and syncytium formation in PEDV-infected Vero cells by cleavage of the PEDV S protein. These findings strongly suggest that the PEDV S protein may undergo a conformational change after receptor binding and cleavage by exogenous trypsin, which induces membrane fusion.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Park, Jung Eun" sort="Park, Jung Eun" uniqKey="Park J" first="Jung-Eun" last="Park">Jung-Eun Park</name></noRegion><name sortKey="Cruz, Deu John M" sort="Cruz, Deu John M" uniqKey="Cruz D" first="Deu John M." last="Cruz">Deu John M. Cruz</name><name sortKey="Shin, Hyun Jin" sort="Shin, Hyun Jin" uniqKey="Shin H" first="Hyun-Jin" last="Shin">Hyun-Jin Shin</name><name sortKey="Shin, Hyun Jin" sort="Shin, Hyun Jin" uniqKey="Shin H" first="Hyun-Jin" last="Shin">Hyun-Jin Shin</name><name sortKey="Shin, Hyun Jin" sort="Shin, Hyun Jin" uniqKey="Shin H" first="Hyun-Jin" last="Shin">Hyun-Jin Shin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002227 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002227 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:F18470B606E38211523ADB3C0CC942C98ED6E6CD
|texte= Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion
}}
| This area was generated with Dilib version V0.6.33. |  |