Interference of ribosomal frameshifting by antisense peptide nucleic acids suppresses SARS coronavirus replication.
Identifieur interne : 002090 ( Main/Exploration ); précédent : 002089; suivant : 002091Interference of ribosomal frameshifting by antisense peptide nucleic acids suppresses SARS coronavirus replication.
Auteurs : Dae-Gyun Ahn [Corée du Sud] ; Wooseong Lee ; Jin-Kyu Choi ; Seong-Jun Kim ; Ewan P. Plant ; Fernando Almazán ; Deborah R. Taylor ; Luis Enjuanes ; Jong-Won OhSource :
- Antiviral research [ 1872-9096 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (génétique), ARN viral (métabolisme), Animaux, Antiviraux (pharmacologie), Cadres ouverts de lecture, Cricetinae, Décalage ribosomique du cadre de lecture, Gènes chevauchants (génétique), Gènes rapporteurs, Génome viral (génétique), Humains, Interféron de type I (biosynthèse), Lignée cellulaire, Luciférases des lucioles, Oligoribonucléotides antisens (génétique), Oligoribonucléotides antisens (métabolisme), Peptides de pénétration cellulaire (génétique), RNA replicase (génétique), RNA replicase (métabolisme), RT-PCR, Réplication virale, Test de retard de migration électrophorétique, Virus du SRAS (génétique), Virus du SRAS (physiologie).
- MESH :
- biosynthèse : Interféron de type I.
- génétique : ARN viral, Gènes chevauchants, Génome viral, Oligoribonucléotides antisens, Peptides de pénétration cellulaire, RNA replicase, Virus du SRAS.
- métabolisme : ARN viral, Oligoribonucléotides antisens, RNA replicase.
- pharmacologie : Antiviraux.
- physiologie : Virus du SRAS.
- ARN viral, Animaux, Cadres ouverts de lecture, Cricetinae, Décalage ribosomique du cadre de lecture, Gènes rapporteurs, Humains, Lignée cellulaire, Luciférases des lucioles, RT-PCR, Réplication virale, Test de retard de migration électrophorétique.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (pharmacology), Cell Line, Cell-Penetrating Peptides (genetics), Cricetinae, Electrophoretic Mobility Shift Assay, Frameshifting, Ribosomal, Genes, Overlapping (genetics), Genes, Reporter, Genome, Viral (genetics), Humans, Interferon Type I (biosynthesis), Luciferases, Firefly, Oligoribonucleotides, Antisense (genetics), Oligoribonucleotides, Antisense (metabolism), Open Reading Frames, RNA Replicase (genetics), RNA Replicase (metabolism), RNA, Viral (chemistry), RNA, Viral (genetics), RNA, Viral (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (physiology), Virus Replication.
- MESH :
- chemical , biosynthesis : Interferon Type I.
- chemical , chemistry : RNA, Viral.
- chemical , genetics : Cell-Penetrating Peptides, Oligoribonucleotides, Antisense, RNA Replicase, RNA, Viral.
- chemical , metabolism : Oligoribonucleotides, Antisense, RNA Replicase, RNA, Viral.
- chemical , pharmacology : Antiviral Agents.
- genetics : Genes, Overlapping, Genome, Viral, SARS Virus.
- physiology : SARS Virus.
- Animals, Cell Line, Cricetinae, Electrophoretic Mobility Shift Assay, Frameshifting, Ribosomal, Genes, Reporter, Humans, Luciferases, Firefly, Open Reading Frames, Reverse Transcriptase Polymerase Chain Reaction, Virus Replication.
Abstract
The programmed -1 ribosomal frameshifting (-1 PRF) utilized by eukaryotic RNA viruses plays a crucial role for the controlled, limited synthesis of viral RNA replicase polyproteins required for genome replication. The viral RNA replicase polyproteins of severe acute respiratory syndrome coronavirus (SARS-CoV) are encoded by the two overlapping open reading frames 1a and 1b, which are connected by a -1 PRF signal. We evaluated the antiviral effects of antisense peptide nucleic acids (PNAs) targeting a highly conserved RNA sequence on the - PRF signal. The ribosomal frameshifting was inhibited by the PNA, which bound sequence-specifically a pseudoknot structure in the -1 PRF signal, in cell lines as assessed using a dual luciferase-based reporter plasmid containing the -1 PRF signal. Treatment of cells, which were transfected with a SARS-CoV-replicon expressing firefly luciferase, with the PNA fused to a cell-penetrating peptide (CPP) resulted in suppression of the replication of the SARS-CoV replicon, with a 50% inhibitory concentration of 4.4μM. There was no induction of type I interferon responses by PNA treatment, suggesting that the effect of PNA is not due to innate immune responses. Our results demonstrate that -1 PRF, critical for SARS-CoV viral replication, can be inhibited by CPP-PNA, providing an effective antisense strategy for blocking -1 PRF signals.
DOI: 10.1016/j.antiviral.2011.04.009
PubMed: 21549154
Affiliations:
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Firefly</term><term>Oligoribonucleotides, Antisense (genetics)</term><term>Oligoribonucleotides, Antisense (metabolism)</term><term>Open Reading Frames</term><term>RNA Replicase (genetics)</term><term>RNA Replicase (metabolism)</term><term>RNA, Viral (chemistry)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (physiology)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term><term>ARN viral (génétique)</term><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Antiviraux (pharmacologie)</term><term>Cadres ouverts de lecture</term><term>Cricetinae</term><term>Décalage ribosomique du cadre de lecture</term><term>Gènes chevauchants (génétique)</term><term>Gènes rapporteurs</term><term>Génome viral (génétique)</term><term>Humains</term><term>Interféron de type I (biosynthèse)</term><term>Lignée cellulaire</term><term>Luciférases des lucioles</term><term>Oligoribonucléotides antisens (génétique)</term><term>Oligoribonucléotides antisens (métabolisme)</term><term>Peptides de pénétration cellulaire (génétique)</term><term>RNA replicase (génétique)</term><term>RNA replicase (métabolisme)</term><term>RT-PCR</term><term>Réplication virale</term><term>Test de retard de migration électrophorétique</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon Type I</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cell-Penetrating Peptides</term><term>Oligoribonucleotides, Antisense</term><term>RNA Replicase</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Oligoribonucleotides, Antisense</term><term>RNA Replicase</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron de type I</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Overlapping</term><term>Genome, Viral</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Gènes chevauchants</term><term>Génome viral</term><term>Oligoribonucléotides antisens</term><term>Peptides de pénétration cellulaire</term><term>RNA replicase</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term><term>Oligoribonucléotides antisens</term><term>RNA replicase</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cricetinae</term><term>Electrophoretic Mobility Shift Assay</term><term>Frameshifting, Ribosomal</term><term>Genes, Reporter</term><term>Humans</term><term>Luciferases, Firefly</term><term>Open Reading Frames</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Cricetinae</term><term>Décalage ribosomique du cadre de lecture</term><term>Gènes rapporteurs</term><term>Humains</term><term>Lignée cellulaire</term><term>Luciférases des lucioles</term><term>RT-PCR</term><term>Réplication virale</term><term>Test de retard de migration électrophorétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The programmed -1 ribosomal frameshifting (-1 PRF) utilized by eukaryotic RNA viruses plays a crucial role for the controlled, limited synthesis of viral RNA replicase polyproteins required for genome replication. The viral RNA replicase polyproteins of severe acute respiratory syndrome coronavirus (SARS-CoV) are encoded by the two overlapping open reading frames 1a and 1b, which are connected by a -1 PRF signal. We evaluated the antiviral effects of antisense peptide nucleic acids (PNAs) targeting a highly conserved RNA sequence on the - PRF signal. The ribosomal frameshifting was inhibited by the PNA, which bound sequence-specifically a pseudoknot structure in the -1 PRF signal, in cell lines as assessed using a dual luciferase-based reporter plasmid containing the -1 PRF signal. Treatment of cells, which were transfected with a SARS-CoV-replicon expressing firefly luciferase, with the PNA fused to a cell-penetrating peptide (CPP) resulted in suppression of the replication of the SARS-CoV replicon, with a 50% inhibitory concentration of 4.4μM. There was no induction of type I interferon responses by PNA treatment, suggesting that the effect of PNA is not due to innate immune responses. Our results demonstrate that -1 PRF, critical for SARS-CoV viral replication, can be inhibited by CPP-PNA, providing an effective antisense strategy for blocking -1 PRF signals.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><noCountry><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name><name sortKey="Choi, Jin Kyu" sort="Choi, Jin Kyu" uniqKey="Choi J" first="Jin-Kyu" last="Choi">Jin-Kyu Choi</name><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name><name sortKey="Kim, Seong Jun" sort="Kim, Seong Jun" uniqKey="Kim S" first="Seong-Jun" last="Kim">Seong-Jun Kim</name><name sortKey="Lee, Wooseong" sort="Lee, Wooseong" uniqKey="Lee W" first="Wooseong" last="Lee">Wooseong Lee</name><name sortKey="Oh, Jong Won" sort="Oh, Jong Won" uniqKey="Oh J" first="Jong-Won" last="Oh">Jong-Won Oh</name><name sortKey="Plant, Ewan P" sort="Plant, Ewan P" uniqKey="Plant E" first="Ewan P" last="Plant">Ewan P. Plant</name><name sortKey="Taylor, Deborah R" sort="Taylor, Deborah R" uniqKey="Taylor D" first="Deborah R" last="Taylor">Deborah R. Taylor</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Ahn, Dae Gyun" sort="Ahn, Dae Gyun" uniqKey="Ahn D" first="Dae-Gyun" last="Ahn">Dae-Gyun Ahn</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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