Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions
Identifieur interne : 001F61 ( Main/Exploration ); précédent : 001F60; suivant : 001F62Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions
Auteurs : Lisa Mullis [États-Unis] ; Linda J. Saif [États-Unis] ; YONGBIN ZHANG [États-Unis] ; XUMING ZHANG [États-Unis] ; Marli S. P. Azevedo [États-Unis]Source :
- Food microbiology [ 0740-0020 ] ; 2012.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Bovin.
English descriptors
Abstract
Fecal suspensions with an aerosol route of transmission were responsible for a cluster of severe acute respiratory syndrome (SARS) cases in 2003 in Hong Kong. Based on that event, the World Health Organization recommended that research be implemented to define modes of transmission of SARS coronavirus through sewage, feces, food and water. Environmental studies have shown that animal coronaviruses remain infectious in water and sewage for up to a year depending on the temperature and humidity. In this study, we examined coronavirus stability on lettuce surfaces. A cell culture adapted bovine coronavirus, diluted in growth media or in bovine fecal suspensions to simulate fecal contamination was used to spike romaine lettuce. qRT-PCR detected viral RNA copy number ranging from 6.6 x 104 to 1.7 x 106 throughout the experimental period of 30 days. Whereas infectious viruses were detected for at least 14 days, the amount of infectious virus varied, depending upon the diluent used for spiking the lettuce. UV and confocal microscopic observation indicated attachment of residual labeled virions to the lettuce surface after the elution procedure, suggesting that rates of inactivation or detection of the virus may be underestimated. Thus, it is possible that contaminated vegetables may be potential vehicles for coronavirus zoonotic transmission to humans.
Affiliations:
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Le document en format XML
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Saif</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Food Animal Health Research Program, OARDC, The Ohio State University</s1><s2>Wooster, OH 44691</s2><s3>USA</s3><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Yongbin Zhang" sort="Yongbin Zhang" uniqKey="Yongbin Zhang" last="Yongbin Zhang">YONGBIN ZHANG</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Center for Toxicological Research, Food and Drug Administration</s1><s2>Jefferson, AR 72079</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Arkansas</region></placeName></affiliation></author><author><name sortKey="Xuming Zhang" sort="Xuming Zhang" uniqKey="Xuming Zhang" last="Xuming Zhang">XUMING ZHANG</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Department of Microbiology and Immunology, University of Arkansas for Medical Science</s1><s2>Little Rock, AR 72205</s2><s3>USA</s3><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Arkansas</region></placeName></affiliation></author><author><name sortKey="Azevedo, Marli S P" sort="Azevedo, Marli S P" uniqKey="Azevedo M" first="Marli S. P." last="Azevedo">Marli S. P. Azevedo</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Center for Toxicological Research, Food and Drug Administration</s1><s2>Jefferson, AR 72079</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Arkansas</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Food microbiology</title><title level="j" type="abbreviated">Food microbiol.</title><idno type="ISSN">0740-0020</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Food microbiology</title><title level="j" type="abbreviated">Food microbiol.</title><idno type="ISSN">0740-0020</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bovine</term><term>Lettuce</term><term>Quality</term><term>Refrigeration</term><term>Stability</term><term>Surface</term><term>Survival</term><term>Virus</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Stabilité</term><term>Bovin</term><term>Laitue</term><term>Virus</term><term>Surface</term><term>Réfrigération</term><term>Survie</term><term>Qualité</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Bovin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fecal suspensions with an aerosol route of transmission were responsible for a cluster of severe acute respiratory syndrome (SARS) cases in 2003 in Hong Kong. Based on that event, the World Health Organization recommended that research be implemented to define modes of transmission of SARS coronavirus through sewage, feces, food and water. Environmental studies have shown that animal coronaviruses remain infectious in water and sewage for up to a year depending on the temperature and humidity. In this study, we examined coronavirus stability on lettuce surfaces. A cell culture adapted bovine coronavirus, diluted in growth media or in bovine fecal suspensions to simulate fecal contamination was used to spike romaine lettuce. qRT-PCR detected viral RNA copy number ranging from 6.6 x 10<sup>4</sup> to 1.7 x 10<sup>6</sup> throughout the experimental period of 30 days. Whereas infectious viruses were detected for at least 14 days, the amount of infectious virus varied, depending upon the diluent used for spiking the lettuce. UV and confocal microscopic observation indicated attachment of residual labeled virions to the lettuce surface after the elution procedure, suggesting that rates of inactivation or detection of the virus may be underestimated. Thus, it is possible that contaminated vegetables may be potential vehicles for coronavirus zoonotic transmission to humans.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Arkansas</li><li>Ohio</li></region></list><tree><country name="États-Unis"><region name="Arkansas"><name sortKey="Mullis, Lisa" sort="Mullis, Lisa" uniqKey="Mullis L" first="Lisa" last="Mullis">Lisa Mullis</name></region><name sortKey="Azevedo, Marli S P" sort="Azevedo, Marli S P" uniqKey="Azevedo M" first="Marli S. P." last="Azevedo">Marli S. P. Azevedo</name><name sortKey="Saif, Linda J" sort="Saif, Linda J" uniqKey="Saif L" first="Linda J." last="Saif">Linda J. Saif</name><name sortKey="Xuming Zhang" sort="Xuming Zhang" uniqKey="Xuming Zhang" last="Xuming Zhang">XUMING ZHANG</name><name sortKey="Yongbin Zhang" sort="Yongbin Zhang" uniqKey="Yongbin Zhang" last="Yongbin Zhang">YONGBIN ZHANG</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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