Diagnostics of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid antigen using chicken immunoglobulin Y.
Identifieur interne : 001D34 ( Main/Exploration ); précédent : 001D33; suivant : 001D35Diagnostics of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid antigen using chicken immunoglobulin Y.
Auteurs : A. Palaniyappan [Canada] ; D. Das ; S. Kammila ; M R Suresh ; H H SunwooSource :
- Poultry science [ 0032-5791 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux, Escherichia coli (génétique), Femelle, Humains, Immunoglobulines (), Poulets, Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines recombinantes (), Protéines recombinantes (génétique), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Technique de Western (médecine vétérinaire), Test ELISA (), Test ELISA (médecine vétérinaire), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Escherichia coli, Protéines nucléocapside, Protéines recombinantes.
- immunologie : Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- médecine vétérinaire : Technique de Western, Test ELISA.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Femelle, Humains, Immunoglobulines, Poulets, Protéines recombinantes, Souris, Souris de lignée BALB C, Test ELISA.
English descriptors
- KwdEn :
- Animals, Blotting, Western (veterinary), Chickens, Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (veterinary), Escherichia coli (genetics), Female, Humans, Immunoglobulins (chemistry), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Recombinant Proteins (chemistry), Recombinant Proteins (genetics), SARS Virus (immunology), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , chemistry : Immunoglobulins, Recombinant Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : Escherichia coli, Nucleocapsid Proteins, Recombinant Proteins.
- chemical , immunology : Nucleocapsid Proteins, SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay.
- veterinary : Blotting, Western, Enzyme-Linked Immunosorbent Assay.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chickens, Female, Humans, Mice, Mice, Inbred BALB C.
Abstract
The goal of this study was to develop a quantitative detection system for severe acute respiratory syndrome-associated coronavirus (SARS-CoV), targeting the nucleocapsid protein (NP), to determine the presence and degree of infection in suspected individuals. Because the NP is the viral protein shed during infection and its template mRNA is the most abundant subgenomic RNA, it is a suitable candidate for developing antibodies for diagnostic applications. In this study, we have prepared full-length SARS-CoV NP expressed in Escherichia coli and purified. Full-length NP was used for the preparation of mouse monoclonal antibody and chicken polyclonal IgY antibodies for the development of heterosandwich ELISA for early diagnostics of SARS-suspected individuals. The sensitivity of the developed heterosandwich ELISA can detect the viral antigen at 18.5 pg/mL of recombinant NP. This study describes ultrasensitive ELISA using 19B6 monoclonal antibody as the capture antibody and IgY as the detecting antibody against the most abundant SARS-CoV NP antigens. One of the most important findings was the use of inexpensive polyclonal IgY antibody to increase the sensitivity of the detection system for SARS-CoV at the picogram level. Furthermore, the immunoassay of SARS-CoV NP antigen developed could be an effective and sensitive method of diagnosing SARS-suspected individuals during a future SARS-CoV outbreak.
DOI: 10.3382/ps.2011-01916
PubMed: 22334738
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001398
- to stream PubMed, to step Curation: 001398
- to stream PubMed, to step Checkpoint: 001361
- to stream Ncbi, to step Merge: 002460
- to stream Ncbi, to step Curation: 002460
- to stream Ncbi, to step Checkpoint: 002460
- to stream Main, to step Merge: 001D57
- to stream Main, to step Curation: 001D34
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Diagnostics of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid antigen using chicken immunoglobulin Y.</title><author><name sortKey="Palaniyappan, A" sort="Palaniyappan, A" uniqKey="Palaniyappan A" first="A" last="Palaniyappan">A. Palaniyappan</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Pharmacy and Pharmaceutical Sciences, 11304-89 Ave., University of Alberta, Edmonton, AB, Canada T6G 2N8.</nlm:affiliation><country>Canada</country><wicri:regionArea>Faculty of Pharmacy and Pharmaceutical Sciences, 11304-89 Ave., University of Alberta, Edmonton, AB</wicri:regionArea><wicri:noRegion>AB</wicri:noRegion></affiliation></author><author><name sortKey="Das, D" sort="Das, D" uniqKey="Das D" first="D" last="Das">D. Das</name></author><author><name sortKey="Kammila, S" sort="Kammila, S" uniqKey="Kammila S" first="S" last="Kammila">S. Kammila</name></author><author><name sortKey="Suresh, M R" sort="Suresh, M R" uniqKey="Suresh M" first="M R" last="Suresh">M R Suresh</name></author><author><name sortKey="Sunwoo, H H" sort="Sunwoo, H H" uniqKey="Sunwoo H" first="H H" last="Sunwoo">H H Sunwoo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22334738</idno><idno type="pmid">22334738</idno><idno type="doi">10.3382/ps.2011-01916</idno><idno type="wicri:Area/PubMed/Corpus">001398</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001398</idno><idno type="wicri:Area/PubMed/Curation">001398</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001398</idno><idno type="wicri:Area/PubMed/Checkpoint">001361</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001361</idno><idno type="wicri:Area/Ncbi/Merge">002460</idno><idno type="wicri:Area/Ncbi/Curation">002460</idno><idno type="wicri:Area/Ncbi/Checkpoint">002460</idno><idno type="wicri:doubleKey">0032-5791:2012:Palaniyappan A:diagnostics:of:severe</idno><idno type="wicri:Area/Main/Merge">001D57</idno><idno type="wicri:Area/Main/Curation">001D34</idno><idno type="wicri:Area/Main/Exploration">001D34</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Diagnostics of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid antigen using chicken immunoglobulin Y.</title><author><name sortKey="Palaniyappan, A" sort="Palaniyappan, A" uniqKey="Palaniyappan A" first="A" last="Palaniyappan">A. Palaniyappan</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Pharmacy and Pharmaceutical Sciences, 11304-89 Ave., University of Alberta, Edmonton, AB, Canada T6G 2N8.</nlm:affiliation><country>Canada</country><wicri:regionArea>Faculty of Pharmacy and Pharmaceutical Sciences, 11304-89 Ave., University of Alberta, Edmonton, AB</wicri:regionArea><wicri:noRegion>AB</wicri:noRegion></affiliation></author><author><name sortKey="Das, D" sort="Das, D" uniqKey="Das D" first="D" last="Das">D. Das</name></author><author><name sortKey="Kammila, S" sort="Kammila, S" uniqKey="Kammila S" first="S" last="Kammila">S. Kammila</name></author><author><name sortKey="Suresh, M R" sort="Suresh, M R" uniqKey="Suresh M" first="M R" last="Suresh">M R Suresh</name></author><author><name sortKey="Sunwoo, H H" sort="Sunwoo, H H" uniqKey="Sunwoo H" first="H H" last="Sunwoo">H H Sunwoo</name></author></analytic><series><title level="j">Poultry science</title><idno type="ISSN">0032-5791</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western (veterinary)</term><term>Chickens</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (veterinary)</term><term>Escherichia coli (genetics)</term><term>Female</term><term>Humans</term><term>Immunoglobulins (chemistry)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Escherichia coli (génétique)</term><term>Femelle</term><term>Humains</term><term>Immunoglobulines ()</term><term>Poulets</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (génétique)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique de Western (médecine vétérinaire)</term><term>Test ELISA ()</term><term>Test ELISA (médecine vétérinaire)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Immunoglobulins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Technique de Western</term><term>Test ELISA</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Humains</term><term>Immunoglobulines</term><term>Poulets</term><term>Protéines recombinantes</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The goal of this study was to develop a quantitative detection system for severe acute respiratory syndrome-associated coronavirus (SARS-CoV), targeting the nucleocapsid protein (NP), to determine the presence and degree of infection in suspected individuals. Because the NP is the viral protein shed during infection and its template mRNA is the most abundant subgenomic RNA, it is a suitable candidate for developing antibodies for diagnostic applications. In this study, we have prepared full-length SARS-CoV NP expressed in Escherichia coli and purified. Full-length NP was used for the preparation of mouse monoclonal antibody and chicken polyclonal IgY antibodies for the development of heterosandwich ELISA for early diagnostics of SARS-suspected individuals. The sensitivity of the developed heterosandwich ELISA can detect the viral antigen at 18.5 pg/mL of recombinant NP. This study describes ultrasensitive ELISA using 19B6 monoclonal antibody as the capture antibody and IgY as the detecting antibody against the most abundant SARS-CoV NP antigens. One of the most important findings was the use of inexpensive polyclonal IgY antibody to increase the sensitivity of the detection system for SARS-CoV at the picogram level. Furthermore, the immunoassay of SARS-CoV NP antigen developed could be an effective and sensitive method of diagnosing SARS-suspected individuals during a future SARS-CoV outbreak.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Das, D" sort="Das, D" uniqKey="Das D" first="D" last="Das">D. Das</name><name sortKey="Kammila, S" sort="Kammila, S" uniqKey="Kammila S" first="S" last="Kammila">S. Kammila</name><name sortKey="Sunwoo, H H" sort="Sunwoo, H H" uniqKey="Sunwoo H" first="H H" last="Sunwoo">H H Sunwoo</name><name sortKey="Suresh, M R" sort="Suresh, M R" uniqKey="Suresh M" first="M R" last="Suresh">M R Suresh</name></noCountry><country name="Canada"><noRegion><name sortKey="Palaniyappan, A" sort="Palaniyappan, A" uniqKey="Palaniyappan A" first="A" last="Palaniyappan">A. Palaniyappan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D34 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001D34 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22334738
|texte= Diagnostics of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid antigen using chicken immunoglobulin Y.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22334738" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |