A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV.
Identifieur interne : 001A01 ( Main/Exploration ); précédent : 001A00; suivant : 001A02A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV.
Auteurs : Guangyu Zhao [République populaire de Chine] ; Lanying Du ; Cuiqing Ma ; Ye Li ; Lin Li ; Vincent Km Poon ; Lili Wang ; Fei Yu ; Bo-Jian Zheng ; Shibo Jiang ; Yusen ZhouSource :
- Virology journal [ 1743-422X ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (isolement et purification), Anticorps neutralisants (isolement et purification), Antiviraux (isolement et purification), Coloration et marquage (), Coronavirus (), Coronavirus (immunologie), Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Lignée cellulaire, Luciferases (analyse), Luciferases (génétique), Souris, Tests de neutralisation (), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), Évaluation préclinique de médicament ().
- MESH :
- analyse : Luciferases.
- génétique : Glycoprotéine de spicule des coronavirus, Luciferases, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- immunologie : Coronavirus.
- isolement et purification : Anticorps antiviraux, Anticorps neutralisants, Antiviraux.
- métabolisme : Glycoprotéine de spicule des coronavirus.
- Animaux, Coloration et marquage, Coronavirus, Humains, Lignée cellulaire, Souris, Tests de neutralisation, Évaluation préclinique de médicament.
English descriptors
- KwdEn :
- Animals, Antibodies, Neutralizing (isolation & purification), Antibodies, Viral (isolation & purification), Antiviral Agents (isolation & purification), Cell Line, Coronavirus (drug effects), Coronavirus (immunology), Drug Evaluation, Preclinical (methods), HIV-1 (genetics), Humans, Luciferases (analysis), Luciferases (genetics), Mice, Neutralization Tests (methods), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (metabolism), Staining and Labeling (methods).
- MESH :
- chemical , analysis : Luciferases.
- chemical , genetics : Luciferases, Spike Glycoprotein, Coronavirus.
- chemical , isolation & purification : Antibodies, Neutralizing, Antibodies, Viral, Antiviral Agents.
- drug effects : Coronavirus.
- genetics : HIV-1.
- immunology : Coronavirus.
- chemical , metabolism : Spike Glycoprotein, Coronavirus.
- methods : Drug Evaluation, Preclinical, Neutralization Tests, Staining and Labeling.
- Animals, Cell Line, Humans, Mice.
Abstract
Evidence points to the emergence of a novel human coronavirus, Middle East respiratory syndrome coronavirus (MERS-CoV), which causes a severe acute respiratory syndrome (SARS)-like disease. In response, the development of effective vaccines and therapeutics remains a clinical priority. To accomplish this, it is necessary to evaluate neutralizing antibodies and screen for MERS-CoV entry inhibitors.
DOI: 10.1186/1743-422X-10-266
PubMed: 23978242
Affiliations:
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Le document en format XML
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scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term><term>Antiviraux</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Drug Evaluation, Preclinical</term><term>Neutralization Tests</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coloration et marquage</term><term>Coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Souris</term><term>Tests de neutralisation</term><term>Évaluation préclinique de médicament</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Evidence points to the emergence of a novel human coronavirus, Middle East respiratory syndrome coronavirus (MERS-CoV), which causes a severe acute respiratory syndrome (SARS)-like disease. In response, the development of effective vaccines and therapeutics remains a clinical priority. To accomplish this, it is necessary to evaluate neutralizing antibodies and screen for MERS-CoV entry inhibitors.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Li, Lin" sort="Li, Lin" uniqKey="Li L" first="Lin" last="Li">Lin Li</name><name sortKey="Li, Ye" sort="Li, Ye" uniqKey="Li Y" first="Ye" last="Li">Ye Li</name><name sortKey="Ma, Cuiqing" sort="Ma, Cuiqing" uniqKey="Ma C" first="Cuiqing" last="Ma">Cuiqing Ma</name><name sortKey="Poon, Vincent Km" sort="Poon, Vincent Km" uniqKey="Poon V" first="Vincent Km" last="Poon">Vincent Km Poon</name><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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