Differential Cell Line Susceptibility to the Emerging Novel Human Betacoronavirus 2c EMC/2012: Implications for Disease Pathogenesis and Clinical Manifestation
Identifieur interne : 001959 ( Main/Exploration ); précédent : 001958; suivant : 001960Differential Cell Line Susceptibility to the Emerging Novel Human Betacoronavirus 2c EMC/2012: Implications for Disease Pathogenesis and Clinical Manifestation
Auteurs : Jasper Fuk-Woo Chan ; Kwok-Hung Chan ; Garnet Kwan-Yue Choi ; Kelvin Kai-Wang To ; Herman Tse ; Jian-Piao Cai ; Man Lung Yeung ; Vincent Chi-Chung Cheng ; Honglin Chen ; Xiao-Yan Che [République populaire de Chine] ; Susanna Kar-Pui Lau ; Patrick Chiu-Yat Woo ; Kwok-Yung YuenSource :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- anatomopathologie : Infections à coronavirus.
- pathogénicité : Coronavirus.
- physiologie : Coronavirus.
- virologie : Infections à coronavirus.
- Charge virale, Culture virale, Humains, Lignée cellulaire, Réplication virale, Tropisme viral.
English descriptors
- KwdEn :
- MESH :
- pathogenicity : Coronavirus.
- pathology : Coronavirus Infections.
- physiology : Coronavirus.
- virology : Coronavirus Infections.
- Cell Line, Humans, Viral Load, Viral Tropism, Virus Cultivation, Virus Replication.
Abstract
The emerging novel human betacoronavirus 2c EMC/2012 (HCoV-EMC) was recently isolated from patients with severe pneumonia and renal failure and was associated with an unexplained high crude fatality rate of 56%. We performed a cell line susceptibility study with 28 cell lines. HCoV-EMC was found to infect the human respiratory tract (polarized airway epithelium cell line Calu-3, embryonic fibroblast cell line HFL, and lung adenocarcinoma cell line A549), kidney (embryonic kidney cell line HEK), intestinal tract (colorectal adenocarcinoma cell line Caco-2), liver cells (hepatocellular carcinoma cell line Huh-7), and histiocytes (malignant histiocytoma cell line His-1), as evident by detection of high or increasing viral load in culture supernatants, detection of viral nucleoprotein expression by immunostaining, and/or detection of cytopathic effects. Although an infected human neuronal cell line (NT2) and infected monocyte and T lymphocyte cell lines (THP-1, U937, and H9) had increased viral loads, their relatively lower viral production corroborated with absent nucleoprotein expression and cytopathic effects. This range of human tissue tropism is broader than that for all other HCoVs, including SARS coronavirus, HCoV-OC43, HCoV-HKU1, HCoV-229E, and HCoV-NL63, which may explain the high mortality associated with this disease. A recent cell line susceptibility study showed that HCoV-EMC can infect primate, porcine, and bat cells and therefore may jump interspecies barriers. We found that HCoV-EMC can also infect civet lung fibroblast and rabbit kidney cell lines. These findings have important implications for the diagnosis, pathogenesis, and transmission of HCoV-EMC.
Url:
DOI: 10.1093/infdis/jit123
PubMed: 23532101
PubMed Central: 7107374
Affiliations:
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Le document en format XML
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id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line>,<institution>the University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region,</institution></nlm:aff></affiliation></author><author><name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line>,<institution>the University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region,</institution></nlm:aff></affiliation></author><author><name sortKey="Cai, Jian Piao" sort="Cai, Jian Piao" uniqKey="Cai J" first="Jian-Piao" last="Cai">Jian-Piao Cai</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yeung, Man Lung" sort="Yeung, Man Lung" uniqKey="Yeung M" first="Man Lung" last="Yeung">Man Lung Yeung</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation wicri:level="1"><nlm:aff id="af5"><addr-line>Center for Clinical Laboratory</addr-line>,<institution>Zhujiang Hospital, Southern Medical University</institution>,<addr-line>Guangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line>,<institution>the University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region,</institution></nlm:aff></affiliation></author><author><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line>,<institution>the University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region,</institution></nlm:aff></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation><nlm:aff id="af1"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line>,<institution>the University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region,</institution></nlm:aff></affiliation></author></analytic><series><title level="j">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Line</term><term>Coronavirus (pathogenicity)</term><term>Coronavirus (physiology)</term><term>Coronavirus Infections (pathology)</term><term>Coronavirus Infections (virology)</term><term>Humans</term><term>Viral Load</term><term>Viral Tropism</term><term>Virus Cultivation</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Charge virale</term><term>Coronavirus (pathogénicité)</term><term>Coronavirus (physiologie)</term><term>Culture virale</term><term>Humains</term><term>Infections à coronavirus (anatomopathologie)</term><term>Infections à coronavirus (virologie)</term><term>Lignée cellulaire</term><term>Réplication virale</term><term>Tropisme viral</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Humans</term><term>Viral Load</term><term>Viral Tropism</term><term>Virus Cultivation</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Charge virale</term><term>Culture virale</term><term>Humains</term><term>Lignée cellulaire</term><term>Réplication virale</term><term>Tropisme viral</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>The emerging novel human betacoronavirus 2c EMC/2012 (HCoV-EMC) was recently isolated from patients with severe pneumonia and renal failure and was associated with an unexplained high crude fatality rate of 56%. We performed a cell line susceptibility study with 28 cell lines. HCoV-EMC was found to infect the human respiratory tract (polarized airway epithelium cell line Calu-3, embryonic fibroblast cell line HFL, and lung adenocarcinoma cell line A549), kidney (embryonic kidney cell line HEK), intestinal tract (colorectal adenocarcinoma cell line Caco-2), liver cells (hepatocellular carcinoma cell line Huh-7), and histiocytes (malignant histiocytoma cell line His-1), as evident by detection of high or increasing viral load in culture supernatants, detection of viral nucleoprotein expression by immunostaining, and/or detection of cytopathic effects. Although an infected human neuronal cell line (NT2) and infected monocyte and T lymphocyte cell lines (THP-1, U937, and H9) had increased viral loads, their relatively lower viral production corroborated with absent nucleoprotein expression and cytopathic effects. This range of human tissue tropism is broader than that for all other HCoVs, including SARS coronavirus, HCoV-OC43, HCoV-HKU1, HCoV-229E, and HCoV-NL63, which may explain the high mortality associated with this disease. A recent cell line susceptibility study showed that HCoV-EMC can infect primate, porcine, and bat cells and therefore may jump interspecies barriers. We found that HCoV-EMC can also infect civet lung fibroblast and rabbit kidney cell lines. These findings have important implications for the diagnosis, pathogenesis, and transmission of HCoV-EMC.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tyrrell, Da" uniqKey="Tyrrell D">DA Tyrrell</name></author><author><name sortKey="Bynoe, Ml" uniqKey="Bynoe M">ML Bynoe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamre, D" uniqKey="Hamre D">D Hamre</name></author><author><name sortKey="Procknow, Jj" uniqKey="Procknow J">JJ Procknow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS 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sortKey="Lam, Cs" uniqKey="Lam C">CS Lam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Yip, Cc" uniqKey="Yip C">CC Yip</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Cai, Jian Piao" sort="Cai, Jian Piao" uniqKey="Cai J" first="Jian-Piao" last="Cai">Jian-Piao Cai</name><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name><name sortKey="Choi, Garnet Kwan Yue" sort="Choi, Garnet Kwan Yue" uniqKey="Choi G" first="Garnet Kwan-Yue" last="Choi">Garnet Kwan-Yue Choi</name><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><name sortKey="Yeung, Man Lung" sort="Yeung, Man Lung" uniqKey="Yeung M" first="Man Lung" last="Yeung">Man Lung Yeung</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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