Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses.
Identifieur interne : 001332 ( Main/Exploration ); précédent : 001331; suivant : 001333Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses.
Auteurs : Lei Shi [République populaire de Chine] ; Qiuxiang Sun [République populaire de Chine] ; Jian'An He [République populaire de Chine] ; Hua Xu [République populaire de Chine] ; Chunxiao Liu [République populaire de Chine] ; Chunzhong Zhao [République populaire de Chine] ; Yunqing Xu [République populaire de Chine] ; Changlin Wu [République populaire de Chine] ; Junjian Xiang [République populaire de Chine] ; Dayong Gu [République populaire de Chine] ; Jun Long [République populaire de Chine] ; Hekui Lan [République populaire de Chine]Source :
- Bio-medical materials and engineering [ 1878-3619 ] ; 2015.
Descripteurs français
- KwdFr :
- Adenoviridae (génétique), Adenoviridae (isolement et purification), Grippe humaine (diagnostic), Grippe humaine (virologie), Humains, Infections à Adenoviridae (diagnostic), Infections à Adenoviridae (virologie), Infections à respirovirus (diagnostic), Infections à respirovirus (virologie), Infections à virus respiratoire syncytial (diagnostic), Infections à virus respiratoire syncytial (virologie), Influenzavirus B (génétique), Influenzavirus B (isolement et purification), Respirovirus (génétique), Respirovirus (isolement et purification), Résonance plasmonique de surface (), Sensibilité et spécificité, Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (isolement et purification), Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Séquençage par oligonucléotides en batterie, Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification), Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Virus respiratoires syncytiaux (génétique), Virus respiratoires syncytiaux (isolement et purification).
- MESH :
- diagnostic : Grippe humaine, Infections à Adenoviridae, Infections à respirovirus, Infections à virus respiratoire syncytial, Syndrome respiratoire aigu sévère.
- génétique : Adenoviridae, Influenzavirus B, Respirovirus, Sous-type H1N1 du virus de la grippe A, Virus de la grippe A, Virus du SRAS, Virus respiratoires syncytiaux.
- isolement et purification : Adenoviridae, Influenzavirus B, Respirovirus, Sous-type H1N1 du virus de la grippe A, Virus de la grippe A, Virus du SRAS, Virus respiratoires syncytiaux.
- virologie : Grippe humaine, Infections à Adenoviridae, Infections à respirovirus, Infections à virus respiratoire syncytial, Syndrome respiratoire aigu sévère.
- Humains, Résonance plasmonique de surface, Sensibilité et spécificité, Séquençage par oligonucléotides en batterie.
English descriptors
- KwdEn :
- Adenoviridae (genetics), Adenoviridae (isolation & purification), Adenoviridae Infections (diagnosis), Adenoviridae Infections (virology), Humans, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (virology), Influenzavirus B (genetics), Influenzavirus B (isolation & purification), Oligonucleotide Array Sequence Analysis, Respiratory Syncytial Virus Infections (diagnosis), Respiratory Syncytial Virus Infections (virology), Respiratory Syncytial Viruses (genetics), Respiratory Syncytial Viruses (isolation & purification), Respirovirus (genetics), Respirovirus (isolation & purification), Respirovirus Infections (diagnosis), Respirovirus Infections (virology), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology), Surface Plasmon Resonance (methods).
- MESH :
- diagnosis : Adenoviridae Infections, Influenza, Human, Respiratory Syncytial Virus Infections, Respirovirus Infections, Severe Acute Respiratory Syndrome.
- genetics : Adenoviridae, Influenza A Virus, H1N1 Subtype, Influenza A virus, Influenzavirus B, Respiratory Syncytial Viruses, Respirovirus, SARS Virus.
- isolation & purification : Adenoviridae, Influenza A Virus, H1N1 Subtype, Influenza A virus, Influenzavirus B, Respiratory Syncytial Viruses, Respirovirus, SARS Virus.
- methods : Surface Plasmon Resonance.
- virology : Adenoviridae Infections, Influenza, Human, Respiratory Syncytial Virus Infections, Respirovirus Infections, Severe Acute Respiratory Syndrome.
- Humans, Oligonucleotide Array Sequence Analysis, Sensitivity and Specificity.
Abstract
A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR biosensor was developed by immobilizing nine respiratory virus-specific oligonucleotides in an SPR chip. To increase the biosensor sensitivity, biotin was used to label the PCR primer and further amplify the signal by introducing streptavidin after hybridization. Throat swab specimens representing nine common respiratory viruses were tested by the innovative SPR-based biosensor to evaluate the sensitivity, specificity and reproducibility of this method. Results suggest that this biosensor has the potential to simultaneously identify common respiratory viruses.
DOI: 10.3233/BME-151526
PubMed: 26406000
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou, 510632</wicri:regionArea><wicri:noRegion>510632</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Qiuxiang" sort="Sun, Qiuxiang" uniqKey="Sun Q" first="Qiuxiang" last="Sun">Qiuxiang Sun</name><affiliation wicri:level="1"><nlm:affiliation>Feng Gang Hospital, Dongguan, 523695, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Feng Gang Hospital, Dongguan, 523695</wicri:regionArea><wicri:noRegion>523695</wicri:noRegion></affiliation></author><author><name sortKey="He, Jian An" sort="He, Jian An" uniqKey="He J" first="Jian'An" last="He">Jian'An He</name><affiliation wicri:level="1"><nlm:affiliation>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033</wicri:regionArea><wicri:noRegion>518033</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Hua" sort="Xu, Hua" uniqKey="Xu H" first="Hua" last="Xu">Hua Xu</name><affiliation wicri:level="1"><nlm:affiliation>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033</wicri:regionArea><wicri:noRegion>518033</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Chunxiao" sort="Liu, Chunxiao" uniqKey="Liu C" first="Chunxiao" last="Liu">Chunxiao Liu</name><affiliation wicri:level="1"><nlm:affiliation>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033</wicri:regionArea><wicri:noRegion>518033</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Chunzhong" sort="Zhao, Chunzhong" uniqKey="Zhao C" first="Chunzhong" last="Zhao">Chunzhong Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033</wicri:regionArea><wicri:noRegion>518033</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Yunqing" sort="Xu, Yunqing" uniqKey="Xu Y" first="Yunqing" last="Xu">Yunqing Xu</name><affiliation wicri:level="1"><nlm:affiliation>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033</wicri:regionArea><wicri:noRegion>518033</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Changlin" sort="Wu, Changlin" uniqKey="Wu C" first="Changlin" last="Wu">Changlin Wu</name><affiliation wicri:level="1"><nlm:affiliation>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033</wicri:regionArea><wicri:noRegion>518033</wicri:noRegion></affiliation></author><author><name sortKey="Xiang, Junjian" sort="Xiang, Junjian" uniqKey="Xiang J" first="Junjian" last="Xiang">Junjian Xiang</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou, 510632, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou, 510632</wicri:regionArea><wicri:noRegion>510632</wicri:noRegion></affiliation></author><author><name sortKey="Gu, Dayong" sort="Gu, Dayong" uniqKey="Gu D" first="Dayong" last="Gu">Dayong Gu</name><affiliation wicri:level="1"><nlm:affiliation>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033</wicri:regionArea><wicri:noRegion>518033</wicri:noRegion></affiliation></author><author><name sortKey="Long, Jun" sort="Long, Jun" uniqKey="Long J" first="Jun" last="Long">Jun Long</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282</wicri:regionArea><wicri:noRegion>510282</wicri:noRegion></affiliation></author><author><name sortKey="Lan, Hekui" sort="Lan, Hekui" uniqKey="Lan H" first="Hekui" last="Lan">Hekui Lan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282</wicri:regionArea><wicri:noRegion>510282</wicri:noRegion></affiliation></author></analytic><series><title level="j">Bio-medical materials and engineering</title><idno type="eISSN">1878-3619</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae (genetics)</term><term>Adenoviridae (isolation & purification)</term><term>Adenoviridae Infections (diagnosis)</term><term>Adenoviridae Infections (virology)</term><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (genetics)</term><term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (isolation & purification)</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (virology)</term><term>Influenzavirus B (genetics)</term><term>Influenzavirus B (isolation & purification)</term><term>Oligonucleotide Array Sequence Analysis</term><term>Respiratory Syncytial Virus Infections (diagnosis)</term><term>Respiratory Syncytial Virus Infections (virology)</term><term>Respiratory Syncytial Viruses (genetics)</term><term>Respiratory Syncytial Viruses (isolation & purification)</term><term>Respirovirus (genetics)</term><term>Respirovirus (isolation & purification)</term><term>Respirovirus Infections (diagnosis)</term><term>Respirovirus Infections (virology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Surface Plasmon Resonance (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adenoviridae (génétique)</term><term>Adenoviridae (isolement et purification)</term><term>Grippe humaine (diagnostic)</term><term>Grippe humaine (virologie)</term><term>Humains</term><term>Infections à Adenoviridae (diagnostic)</term><term>Infections à Adenoviridae (virologie)</term><term>Infections à respirovirus (diagnostic)</term><term>Infections à respirovirus (virologie)</term><term>Infections à virus respiratoire syncytial (diagnostic)</term><term>Infections à virus respiratoire syncytial (virologie)</term><term>Influenzavirus B (génétique)</term><term>Influenzavirus B (isolement et purification)</term><term>Respirovirus (génétique)</term><term>Respirovirus (isolement et purification)</term><term>Résonance plasmonique de surface ()</term><term>Sensibilité et spécificité</term><term>Sous-type H1N1 du virus de la grippe A (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (isolement et purification)</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Séquençage par oligonucléotides en batterie</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus respiratoires syncytiaux (génétique)</term><term>Virus respiratoires syncytiaux (isolement et purification)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Adenoviridae Infections</term><term>Influenza, Human</term><term>Respiratory Syncytial Virus Infections</term><term>Respirovirus Infections</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term><term>Infections à Adenoviridae</term><term>Infections à respirovirus</term><term>Infections à virus respiratoire syncytial</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviridae</term><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A virus</term><term>Influenzavirus B</term><term>Respiratory Syncytial Viruses</term><term>Respirovirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adenoviridae</term><term>Influenzavirus B</term><term>Respirovirus</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Virus de la grippe A</term><term>Virus du SRAS</term><term>Virus respiratoires syncytiaux</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Adenoviridae</term><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A virus</term><term>Influenzavirus B</term><term>Respiratory Syncytial Viruses</term><term>Respirovirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Adenoviridae</term><term>Influenzavirus B</term><term>Respirovirus</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Virus de la grippe A</term><term>Virus du SRAS</term><term>Virus respiratoires syncytiaux</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Surface Plasmon Resonance</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term><term>Infections à Adenoviridae</term><term>Infections à respirovirus</term><term>Infections à virus respiratoire syncytial</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Adenoviridae Infections</term><term>Influenza, Human</term><term>Respiratory Syncytial Virus Infections</term><term>Respirovirus Infections</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Oligonucleotide Array Sequence Analysis</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Résonance plasmonique de surface</term><term>Sensibilité et spécificité</term><term>Séquençage par oligonucléotides en batterie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR biosensor was developed by immobilizing nine respiratory virus-specific oligonucleotides in an SPR chip. To increase the biosensor sensitivity, biotin was used to label the PCR primer and further amplify the signal by introducing streptavidin after hybridization. Throat swab specimens representing nine common respiratory viruses were tested by the innovative SPR-based biosensor to evaluate the sensitivity, specificity and reproducibility of this method. Results suggest that this biosensor has the potential to simultaneously identify common respiratory viruses. </div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Shi, Lei" sort="Shi, Lei" uniqKey="Shi L" first="Lei" last="Shi">Lei Shi</name></noRegion><name sortKey="Gu, Dayong" sort="Gu, Dayong" uniqKey="Gu D" first="Dayong" last="Gu">Dayong Gu</name><name sortKey="He, Jian An" sort="He, Jian An" uniqKey="He J" first="Jian'An" last="He">Jian'An He</name><name sortKey="Lan, Hekui" sort="Lan, Hekui" uniqKey="Lan H" first="Hekui" last="Lan">Hekui Lan</name><name sortKey="Liu, Chunxiao" sort="Liu, Chunxiao" uniqKey="Liu C" first="Chunxiao" last="Liu">Chunxiao Liu</name><name sortKey="Long, Jun" sort="Long, Jun" uniqKey="Long J" first="Jun" last="Long">Jun Long</name><name sortKey="Sun, Qiuxiang" sort="Sun, Qiuxiang" uniqKey="Sun Q" first="Qiuxiang" last="Sun">Qiuxiang Sun</name><name sortKey="Wu, Changlin" sort="Wu, Changlin" uniqKey="Wu C" first="Changlin" last="Wu">Changlin Wu</name><name sortKey="Xiang, Junjian" sort="Xiang, Junjian" uniqKey="Xiang J" first="Junjian" last="Xiang">Junjian Xiang</name><name sortKey="Xu, Hua" sort="Xu, Hua" uniqKey="Xu H" first="Hua" last="Xu">Hua Xu</name><name sortKey="Xu, Yunqing" sort="Xu, Yunqing" uniqKey="Xu Y" first="Yunqing" last="Xu">Yunqing Xu</name><name sortKey="Zhao, Chunzhong" sort="Zhao, Chunzhong" uniqKey="Zhao C" first="Chunzhong" last="Zhao">Chunzhong Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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