Interactions among SARS-CoV accessory proteins revealed by bimolecular fluorescence complementation assay
Identifieur interne : 001296 ( Main/Exploration ); précédent : 001295; suivant : 001297Interactions among SARS-CoV accessory proteins revealed by bimolecular fluorescence complementation assay
Auteurs : Jianqiang Kong [République populaire de Chine] ; Yanwei Shi [République populaire de Chine] ; Zhifang Wang [République populaire de Chine] ; Yiting Pan [République populaire de Chine]Source :
- Acta Pharmaceutica Sinica. B [ 2211-3835 ] ; 2015.
Abstract
The accessory proteins (3a, 3b, 6, 7a, 7b, 8a, 8b, 9b and ORF14), predicted unknown proteins (PUPs) encoded by the genes, are considered to be unique to the severe acute respiratory syndrome coronavirus (SARS-CoV) genome. These proteins play important roles in various biological processes mediated by interactions with their partners. However, very little is known about the interactions among these accessory proteins. Here, a EYFP (enhanced yellow fluorescent protein) bimolecular fluorescence complementation (BiFC) assay was used to detect the interactions among accessory proteins. 33 out of 81 interactions were identified by BiFC, much more than that identified by the yeast two-hybrid (Y2H) system. This is the first report describing direct visualization of interactions among accessory proteins of SARS-CoV. These findings attest to the general applicability of the BiFC system for the verification of protein-protein interactions.
Url:
DOI: 10.1016/j.apsb.2015.05.002
PubMed: 26579480
PubMed Central: 4629423
Affiliations:
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Kong</name><affiliation wicri:level="1"><nlm:aff id="aff0005">State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shi, Yanwei" sort="Shi, Yanwei" uniqKey="Shi Y" first="Yanwei" last="Shi">Yanwei Shi</name><affiliation wicri:level="1"><nlm:aff id="aff0005">State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Zhifang" sort="Wang, Zhifang" uniqKey="Wang Z" first="Zhifang" last="Wang">Zhifang Wang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Pan, Yiting" sort="Pan, Yiting" uniqKey="Pan Y" first="Yiting" last="Pan">Yiting Pan</name><affiliation wicri:level="1"><nlm:aff id="aff0010">School of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Acta Pharmaceutica Sinica. B</title><idno type="ISSN">2211-3835</idno><idno type="eISSN">2211-3843</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The accessory proteins (3a, 3b, 6, 7a, 7b, 8a, 8b, 9b and ORF14), predicted unknown proteins (PUPs) encoded by the genes, are considered to be unique to the severe acute respiratory syndrome coronavirus (SARS-CoV) genome. These proteins play important roles in various biological processes mediated by interactions with their partners. However, very little is known about the interactions among these accessory proteins. Here, a EYFP (enhanced yellow fluorescent protein) bimolecular fluorescence complementation (BiFC) assay was used to detect the interactions among accessory proteins. 33 out of 81 interactions were identified by BiFC, much more than that identified by the yeast two-hybrid (Y2H) system. This is the first report describing direct visualization of interactions among accessory proteins of SARS-CoV. 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