The Nucleocapsid Protein of Coronaviruses Acts as a Viral Suppressor of RNA Silencing in Mammalian Cells
Identifieur interne : 001222 ( Main/Exploration ); précédent : 001221; suivant : 001223The Nucleocapsid Protein of Coronaviruses Acts as a Viral Suppressor of RNA Silencing in Mammalian Cells
Auteurs : Lei Cui ; Haiying Wang ; Yanxi Ji ; Jie Yang ; Shan Xu ; Xingyu Huang ; Zidao Wang ; Lei Qin ; Po Tien ; Xi Zhou ; Deyin Guo ; Yu ChenSource :
- Journal of Virology [ 0022-538X ] ; 2015.
Descripteurs français
- KwdFr :
- Alignement de séquences, Animaux, Cellules HEK293, Coronavirus (génétique), Coronavirus (immunologie), DEAD-box RNA helicases (génétique), Humains, Interférence par ARN, Lignée cellulaire, Petit ARN interférent (génétique), Protéines Argonaute (génétique), Protéines nucléocapside (biosynthèse), Protéines nucléocapside (génétique), Ribonuclease III (génétique), Souris, Syndrome respiratoire aigu sévère (génétique), Syndrome respiratoire aigu sévère (virologie), Séquence d'acides aminés, Séquence nucléotidique, Virus de l'hépatite murine (croissance et développement), Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (immunologie).
- MESH :
- biosynthèse : Protéines nucléocapside.
- croissance et développement : Virus de l'hépatite murine.
- génétique : Coronavirus, DEAD-box RNA helicases, Petit ARN interférent, Protéines Argonaute, Protéines nucléocapside, Ribonuclease III, Syndrome respiratoire aigu sévère, Virus de l'hépatite murine.
- immunologie : Coronavirus, Virus de l'hépatite murine.
- virologie : Syndrome respiratoire aigu sévère.
- Alignement de séquences, Animaux, Cellules HEK293, Humains, Interférence par ARN, Lignée cellulaire, Souris, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Argonaute Proteins (genetics), Base Sequence, Cell Line, Coronavirus (genetics), Coronavirus (immunology), DEAD-box RNA Helicases (genetics), HEK293 Cells, Humans, L Cells, Mice, Murine hepatitis virus (genetics), Murine hepatitis virus (growth & development), Murine hepatitis virus (immunology), Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), RNA Interference, RNA, Small Interfering (genetics), Ribonuclease III (genetics), Sequence Alignment, Severe Acute Respiratory Syndrome (genetics), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , biosynthesis : Nucleocapsid Proteins.
- chemical , genetics : Argonaute Proteins, DEAD-box RNA Helicases, Nucleocapsid Proteins, RNA, Small Interfering, Ribonuclease III.
- genetics : Coronavirus, Murine hepatitis virus, Severe Acute Respiratory Syndrome.
- growth & development : Murine hepatitis virus.
- immunology : Coronavirus, Murine hepatitis virus.
- virology : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, HEK293 Cells, Humans, L Cells, Mice, RNA Interference, Sequence Alignment.
Abstract
RNA interference (RNAi) is a process of eukaryotic posttranscriptional gene silencing that functions in antiviral immunity in plants, nematodes, and insects. However, recent studies provided strong supports that RNAi also plays a role in antiviral mechanism in mammalian cells. To combat RNAi-mediated antiviral responses, many viruses encode viral suppressors of RNA silencing (VSR) to facilitate their replication. VSRs have been widely studied for plant and insect viruses, but only a few have been defined for mammalian viruses currently. We identified a novel VSR from coronaviruses, a group of medically important mammalian viruses including Severe acute respiratory syndrome coronavirus (SARS-CoV), and showed that the nucleocapsid protein (N protein) of coronaviruses suppresses RNAi triggered by either short hairpin RNAs or small interfering RNAs in mammalian cells. Mouse hepatitis virus (MHV) is closely related to SARS-CoV in the family
Url:
DOI: 10.1128/JVI.01331-15
PubMed: 26085159
PubMed Central: 4524063
Affiliations:
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Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Nucleocapsid Protein of Coronaviruses Acts as a Viral Suppressor of RNA Silencing in Mammalian Cells</title>
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<author><name sortKey="Chen, Yu" sort="Chen, Yu" uniqKey="Chen Y" first="Yu" last="Chen">Yu Chen</name>
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<series><title level="j">Journal of Virology</title>
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<term>Animals</term>
<term>Argonaute Proteins (genetics)</term>
<term>Base Sequence</term>
<term>Cell Line</term>
<term>Coronavirus (genetics)</term>
<term>Coronavirus (immunology)</term>
<term>DEAD-box RNA Helicases (genetics)</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>L Cells</term>
<term>Mice</term>
<term>Murine hepatitis virus (genetics)</term>
<term>Murine hepatitis virus (growth & development)</term>
<term>Murine hepatitis virus (immunology)</term>
<term>Nucleocapsid Proteins (biosynthesis)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>RNA Interference</term>
<term>RNA, Small Interfering (genetics)</term>
<term>Ribonuclease III (genetics)</term>
<term>Sequence Alignment</term>
<term>Severe Acute Respiratory Syndrome (genetics)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term>
<term>Animaux</term>
<term>Cellules HEK293</term>
<term>Coronavirus (génétique)</term>
<term>Coronavirus (immunologie)</term>
<term>DEAD-box RNA helicases (génétique)</term>
<term>Humains</term>
<term>Interférence par ARN</term>
<term>Lignée cellulaire</term>
<term>Petit ARN interférent (génétique)</term>
<term>Protéines Argonaute (génétique)</term>
<term>Protéines nucléocapside (biosynthèse)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Ribonuclease III (génétique)</term>
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<term>Syndrome respiratoire aigu sévère (génétique)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Virus de l'hépatite murine (croissance et développement)</term>
<term>Virus de l'hépatite murine (génétique)</term>
<term>Virus de l'hépatite murine (immunologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Argonaute Proteins</term>
<term>DEAD-box RNA Helicases</term>
<term>Nucleocapsid Proteins</term>
<term>RNA, Small Interfering</term>
<term>Ribonuclease III</term>
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<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines nucléocapside</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term>
<term>Murine hepatitis virus</term>
<term>Severe Acute Respiratory Syndrome</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Murine hepatitis virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term>
<term>DEAD-box RNA helicases</term>
<term>Petit ARN interférent</term>
<term>Protéines Argonaute</term>
<term>Protéines nucléocapside</term>
<term>Ribonuclease III</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus de l'hépatite murine</term>
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<term>Virus de l'hépatite murine</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus</term>
<term>Murine hepatitis virus</term>
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<term>Animals</term>
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>RNA interference (RNAi) is a process of eukaryotic posttranscriptional gene silencing that functions in antiviral immunity in plants, nematodes, and insects. However, recent studies provided strong supports that RNAi also plays a role in antiviral mechanism in mammalian cells. To combat RNAi-mediated antiviral responses, many viruses encode viral suppressors of RNA silencing (VSR) to facilitate their replication. VSRs have been widely studied for plant and insect viruses, but only a few have been defined for mammalian viruses currently. We identified a novel VSR from coronaviruses, a group of medically important mammalian viruses including Severe acute respiratory syndrome coronavirus (SARS-CoV), and showed that the nucleocapsid protein (N protein) of coronaviruses suppresses RNAi triggered by either short hairpin RNAs or small interfering RNAs in mammalian cells. Mouse hepatitis virus (MHV) is closely related to SARS-CoV in the family <named-content content-type="genus-species">Coronaviridae</named-content>
and was used as a coronavirus replication model. The replication of MHV increased when the N proteins were expressed <italic>in trans</italic>
, while knockdown of Dicer1 or Ago2 transcripts facilitated the MHV replication in mammalian cells. These results support the hypothesis that RNAi is a part of the antiviral immunity responses in mammalian cells.</p>
<p><bold>IMPORTANCE</bold>
RNAi has been well known to play important antiviral roles from plants to invertebrates. However, recent studies provided strong supports that RNAi is also involved in antiviral response in mammalian cells. An important indication for RNAi-mediated antiviral activity in mammals is the fact that a number of mammalian viruses encode potent suppressors of RNA silencing. Our results demonstrate that coronavirus N protein could function as a VSR through its double-stranded RNA binding activity. Mutational analysis of N protein allowed us to find out the critical residues for the VSR activity. Using the MHV-A59 as the coronavirus replication model, we showed that ectopic expression of SARS-CoV N protein could promote MHV replication in RNAi-active cells but not in RNAi-depleted cells. These results indicate that coronaviruses encode a VSR that functions in the replication cycle and provide further evidence to support that RNAi-mediated antiviral response exists in mammalian cells.</p>
</div>
</front>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Yu" sort="Chen, Yu" uniqKey="Chen Y" first="Yu" last="Chen">Yu Chen</name>
<name sortKey="Cui, Lei" sort="Cui, Lei" uniqKey="Cui L" first="Lei" last="Cui">Lei Cui</name>
<name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name>
<name sortKey="Huang, Xingyu" sort="Huang, Xingyu" uniqKey="Huang X" first="Xingyu" last="Huang">Xingyu Huang</name>
<name sortKey="Ji, Yanxi" sort="Ji, Yanxi" uniqKey="Ji Y" first="Yanxi" last="Ji">Yanxi Ji</name>
<name sortKey="Qin, Lei" sort="Qin, Lei" uniqKey="Qin L" first="Lei" last="Qin">Lei Qin</name>
<name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name>
<name sortKey="Wang, Haiying" sort="Wang, Haiying" uniqKey="Wang H" first="Haiying" last="Wang">Haiying Wang</name>
<name sortKey="Wang, Zidao" sort="Wang, Zidao" uniqKey="Wang Z" first="Zidao" last="Wang">Zidao Wang</name>
<name sortKey="Xu, Shan" sort="Xu, Shan" uniqKey="Xu S" first="Shan" last="Xu">Shan Xu</name>
<name sortKey="Yang, Jie" sort="Yang, Jie" uniqKey="Yang J" first="Jie" last="Yang">Jie Yang</name>
<name sortKey="Zhou, Xi" sort="Zhou, Xi" uniqKey="Zhou X" first="Xi" last="Zhou">Xi Zhou</name>
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