A recombinant VSV-vectored MERS-CoV vaccine induces neutralizing antibody and T cell responses in rhesus monkeys after single dose immunization.
Identifieur interne : 000D21 ( Main/Exploration ); précédent : 000D20; suivant : 000D22A recombinant VSV-vectored MERS-CoV vaccine induces neutralizing antibody and T cell responses in rhesus monkeys after single dose immunization.
Auteurs : Renqiang Liu [République populaire de Chine] ; Jinliang Wang [République populaire de Chine] ; Yu Shao [République populaire de Chine] ; Xijun Wang [République populaire de Chine] ; Huilei Zhang [République populaire de Chine] ; Lei Shuai [République populaire de Chine] ; Jinying Ge [République populaire de Chine] ; Zhiyuan Wen [République populaire de Chine] ; Zhigao Bu [République populaire de Chine]Source :
- Antiviral research [ 1872-9096 ] ; 2018.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps neutralisants (immunologie), Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Dipeptidyl peptidase 4 (métabolisme), Expression des gènes, Gènes rapporteurs, Humains, Immunoglobuline G (immunologie), Infections à coronavirus (), Lymphocytes T (immunologie), Lymphocytes T (métabolisme), Macaca mulatta, Modèles animaux de maladie humaine, Mâle, Pénétration virale, Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vaccins synthétiques (génétique), Vaccins synthétiques (immunologie), Vecteurs génétiques (génétique), Vecteurs génétiques (immunologie).
- MESH :
- génétique : Vaccins antiviraux, Vaccins synthétiques, Vecteurs génétiques.
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Coronavirus du syndrome respiratoire du Moyen-Orient, Immunoglobuline G, Lymphocytes T, Vaccins antiviraux, Vaccins synthétiques, Vecteurs génétiques.
- métabolisme : Dipeptidyl peptidase 4, Lymphocytes T.
- Animaux, Cellules Vero, Expression des gènes, Gènes rapporteurs, Humains, Infections à coronavirus, Macaca mulatta, Modèles animaux de maladie humaine, Mâle, Pénétration virale.
English descriptors
- KwdEn :
- Animals, Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Chlorocebus aethiops, Coronavirus Infections (prevention & control), Dipeptidyl Peptidase 4 (metabolism), Disease Models, Animal, Gene Expression, Genes, Reporter, Genetic Vectors (genetics), Genetic Vectors (immunology), Humans, Immunoglobulin G (immunology), Macaca mulatta, Male, Middle East Respiratory Syndrome Coronavirus (immunology), T-Lymphocytes (immunology), T-Lymphocytes (metabolism), Vaccines, Synthetic (genetics), Vaccines, Synthetic (immunology), Vero Cells, Viral Vaccines (genetics), Viral Vaccines (immunology), Virus Internalization.
- MESH :
- chemical , genetics : Vaccines, Synthetic, Viral Vaccines.
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral, Immunoglobulin G, Vaccines, Synthetic, Viral Vaccines.
- chemical , metabolism : Dipeptidyl Peptidase 4.
- genetics : Genetic Vectors.
- immunology : Genetic Vectors, Middle East Respiratory Syndrome Coronavirus, T-Lymphocytes.
- metabolism : T-Lymphocytes.
- prevention & control : Coronavirus Infections.
- Animals, Chlorocebus aethiops, Disease Models, Animal, Gene Expression, Genes, Reporter, Humans, Macaca mulatta, Male, Vero Cells, Virus Internalization.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) has been a highly threatening zoonotic pathogen since its outbreak in 2012. Similar to SARS-CoV, MERS-CoV belongs to the coronavirus family and can induce severe respiratory symptoms in humans, with an average case fatality rate of 35% according to the World Health Organization. Spike (S) protein of MERS-CoV is immunogenic and can induce neutralizing antibodies, thus is a potential major target for vaccine development. Here we constructed a chimeric virus based on the vesicular stomatitis virus (VSV) in which the G gene was replaced by MERS-CoV S gene (VSVΔG-MERS). The S protein efficiently incorporated into the viral envelope and mediated cell entry through binding its receptor, human DPP4. Knockdown of clathrin expression by siRNA drastically abrogated the infection of VSVΔG-MERS in Vero cells. Furthermore, in animal studies, the recombinant virus induced neutralizing antibodies and T cell responses in rhesus monkeys after a single intramuscular or intranasal immunization dose. Our findings indicate the potential of the chimeric VSVΔG-MERS as a rapid response vaccine candidate against emerging MERS-CoV disease.
DOI: 10.1016/j.antiviral.2017.12.007
PubMed: 29246504
Affiliations:
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Le document en format XML
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Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Wen, Zhiyuan" sort="Wen, Zhiyuan" uniqKey="Wen Z" first="Zhiyuan" last="Wen">Zhiyuan Wen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China. 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xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Jinliang" sort="Wang, Jinliang" uniqKey="Wang J" first="Jinliang" last="Wang">Jinliang Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Shao, Yu" sort="Shao, Yu" uniqKey="Shao Y" first="Yu" last="Shao">Yu Shao</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xijun" sort="Wang, Xijun" uniqKey="Wang X" first="Xijun" last="Wang">Xijun Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Huilei" sort="Zhang, Huilei" uniqKey="Zhang H" first="Huilei" last="Zhang">Huilei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Shuai, Lei" sort="Shuai, Lei" uniqKey="Shuai L" first="Lei" last="Shuai">Lei Shuai</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Ge, Jinying" sort="Ge, Jinying" uniqKey="Ge J" first="Jinying" last="Ge">Jinying Ge</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Wen, Zhiyuan" sort="Wen, Zhiyuan" uniqKey="Wen Z" first="Zhiyuan" last="Wen">Zhiyuan Wen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China. Electronic address: wenzhiyuan@caas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author><author><name sortKey="Bu, Zhigao" sort="Bu, Zhigao" uniqKey="Bu Z" first="Zhigao" last="Bu">Zhigao Bu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province, PR China. Electronic address: buzhigao@caas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, Heilongjiang Province</wicri:regionArea><wicri:noRegion>Heilongjiang Province</wicri:noRegion></affiliation></author></analytic><series><title level="j">Antiviral research</title><idno type="eISSN">1872-9096</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (prevention & control)</term><term>Dipeptidyl Peptidase 4 (metabolism)</term><term>Disease Models, Animal</term><term>Gene Expression</term><term>Genes, Reporter</term><term>Genetic Vectors (genetics)</term><term>Genetic Vectors (immunology)</term><term>Humans</term><term>Immunoglobulin G (immunology)</term><term>Macaca mulatta</term><term>Male</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>T-Lymphocytes (immunology)</term><term>T-Lymphocytes (metabolism)</term><term>Vaccines, Synthetic (genetics)</term><term>Vaccines, Synthetic (immunology)</term><term>Vero Cells</term><term>Viral Vaccines (genetics)</term><term>Viral Vaccines (immunology)</term><term>Virus Internalization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps neutralisants (immunologie)</term><term>Cellules Vero</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Dipeptidyl peptidase 4 (métabolisme)</term><term>Expression des gènes</term><term>Gènes rapporteurs</term><term>Humains</term><term>Immunoglobuline G (immunologie)</term><term>Infections à coronavirus ()</term><term>Lymphocytes T (immunologie)</term><term>Lymphocytes T (métabolisme)</term><term>Macaca mulatta</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Pénétration virale</term><term>Vaccins antiviraux (génétique)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins synthétiques (génétique)</term><term>Vaccins synthétiques (immunologie)</term><term>Vecteurs génétiques (génétique)</term><term>Vecteurs génétiques (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Vaccines, Synthetic</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Immunoglobulin G</term><term>Vaccines, Synthetic</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genetic Vectors</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vaccins synthétiques</term><term>Vecteurs génétiques</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Immunoglobuline G</term><term>Lymphocytes T</term><term>Vaccins antiviraux</term><term>Vaccins synthétiques</term><term>Vecteurs génétiques</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Genetic Vectors</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dipeptidyl peptidase 4</term><term>Lymphocytes T</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Disease Models, Animal</term><term>Gene Expression</term><term>Genes, Reporter</term><term>Humans</term><term>Macaca mulatta</term><term>Male</term><term>Vero Cells</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Expression des gènes</term><term>Gènes rapporteurs</term><term>Humains</term><term>Infections à coronavirus</term><term>Macaca mulatta</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Pénétration virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) has been a highly threatening zoonotic pathogen since its outbreak in 2012. Similar to SARS-CoV, MERS-CoV belongs to the coronavirus family and can induce severe respiratory symptoms in humans, with an average case fatality rate of 35% according to the World Health Organization. Spike (S) protein of MERS-CoV is immunogenic and can induce neutralizing antibodies, thus is a potential major target for vaccine development. Here we constructed a chimeric virus based on the vesicular stomatitis virus (VSV) in which the G gene was replaced by MERS-CoV S gene (VSVΔG-MERS). The S protein efficiently incorporated into the viral envelope and mediated cell entry through binding its receptor, human DPP4. Knockdown of clathrin expression by siRNA drastically abrogated the infection of VSVΔG-MERS in Vero cells. Furthermore, in animal studies, the recombinant virus induced neutralizing antibodies and T cell responses in rhesus monkeys after a single intramuscular or intranasal immunization dose. Our findings indicate the potential of the chimeric VSVΔG-MERS as a rapid response vaccine candidate against emerging MERS-CoV disease.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Renqiang" sort="Liu, Renqiang" uniqKey="Liu R" first="Renqiang" last="Liu">Renqiang Liu</name></noRegion><name sortKey="Bu, Zhigao" sort="Bu, Zhigao" uniqKey="Bu Z" first="Zhigao" last="Bu">Zhigao Bu</name><name sortKey="Ge, Jinying" sort="Ge, Jinying" uniqKey="Ge J" first="Jinying" last="Ge">Jinying Ge</name><name sortKey="Shao, Yu" sort="Shao, Yu" uniqKey="Shao Y" first="Yu" last="Shao">Yu Shao</name><name sortKey="Shuai, Lei" sort="Shuai, Lei" uniqKey="Shuai L" first="Lei" last="Shuai">Lei Shuai</name><name sortKey="Wang, Jinliang" sort="Wang, Jinliang" uniqKey="Wang J" first="Jinliang" last="Wang">Jinliang Wang</name><name sortKey="Wang, Xijun" sort="Wang, Xijun" uniqKey="Wang X" first="Xijun" last="Wang">Xijun Wang</name><name sortKey="Wen, Zhiyuan" sort="Wen, Zhiyuan" uniqKey="Wen Z" first="Zhiyuan" last="Wen">Zhiyuan Wen</name><name sortKey="Zhang, Huilei" sort="Zhang, Huilei" uniqKey="Zhang H" first="Huilei" last="Zhang">Huilei Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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