SrasV1, Main, Exploration, bibRecord, 000C34

Structural model of the SARS coronavirus E channel in LMPG micelles.

Identifieur interne : 000C34 ( Main/Exploration ); précédent : 000C33; suivant : 000C35

Structural model of the SARS coronavirus E channel in LMPG micelles.

Auteurs : Wahyu Surya [Singapour] ; Yan Li [Singapour] ; Jaume Torres [Singapour]

Source :

Biochimica et biophysica acta. Biomembranes [ 0005-2736 ] ; 2018.

RBID : pubmed:29474890

Descripteurs français

KwdFr :
- Conformation des protéines, Micelles, Modèles moléculaires, Phosphatidylglycérol (), Protéines de l'enveloppe virale (), Relation structure-activité, Résonance magnétique nucléaire biomoléculaire, Virus du SRAS (), Électrophorèse sur gel de polyacrylamide.
MESH :
- Conformation des protéines, Micelles, Modèles moléculaires, Phosphatidylglycérol, Protéines de l'enveloppe virale, Relation structure-activité, Résonance magnétique nucléaire biomoléculaire, Virus du SRAS, Électrophorèse sur gel de polyacrylamide.

English descriptors

KwdEn :
- Electrophoresis, Polyacrylamide Gel, Micelles, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Phosphatidylglycerols (chemistry), Protein Conformation, SARS Virus (chemistry), Structure-Activity Relationship, Viral Envelope Proteins (chemistry).
MESH :
- chemical , chemistry : Phosphatidylglycerols, Viral Envelope Proteins.
- chemical : Micelles.
- chemistry : SARS Virus.
- Electrophoresis, Polyacrylamide Gel, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Protein Conformation, Structure-Activity Relationship.

Abstract

Coronaviruses (CoV) cause common colds in humans, but are also responsible for the recent Severe Acute, and Middle East, respiratory syndromes (SARS and MERS, respectively). A promising approach for prevention are live attenuated vaccines (LAVs), some of which target the envelope (E) protein, which is a small membrane protein that forms ion channels. Unfortunately, detailed structural information is still limited for SARS-CoV E, and non-existent for other CoV E proteins. Herein, we report a structural model of a SARS-CoV E construct in LMPG micelles with, for the first time, unequivocal intermolecular NOEs. The model corresponding to the detergent-embedded region is consistent with previously obtained orientational restraints obtained in lipid bilayers and in vivo escape mutants. The C-terminal domain is mostly α-helical, and extramembrane intermolecular NOEs suggest interactions that may affect the TM channel conformation.

DOI: 10.1016/j.bbamem.2018.02.017
PubMed: 29474890

Affiliations:

Singapour

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural model of the SARS coronavirus E channel in LMPG micelles.</title>
<author><name sortKey="Surya, Wahyu" sort="Surya, Wahyu" uniqKey="Surya W" first="Wahyu" last="Surya">Wahyu Surya</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Torres, Jaume" sort="Torres, Jaume" uniqKey="Torres J" first="Jaume" last="Torres">Jaume Torres</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore. Electronic address: jtorres@ntu.edu.sg.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2018">2018</date>
<idno type="RBID">pubmed:29474890</idno>
<idno type="pmid">29474890</idno>
<idno type="doi">10.1016/j.bbamem.2018.02.017</idno>
<idno type="wicri:Area/PubMed/Corpus">000A25</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A25</idno>
<idno type="wicri:Area/PubMed/Curation">000A25</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000A25</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000961</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000961</idno>
<idno type="wicri:Area/Ncbi/Merge">002F07</idno>
<idno type="wicri:Area/Ncbi/Curation">002F07</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002F07</idno>
<idno type="wicri:doubleKey">0005-2736:2018:Surya W:structural:model:of</idno>
<idno type="wicri:Area/Main/Merge">000C36</idno>
<idno type="wicri:Area/Main/Curation">000C34</idno>
<idno type="wicri:Area/Main/Exploration">000C34</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Structural model of the SARS coronavirus E channel in LMPG micelles.</title>
<author><name sortKey="Surya, Wahyu" sort="Surya, Wahyu" uniqKey="Surya W" first="Wahyu" last="Surya">Wahyu Surya</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Torres, Jaume" sort="Torres, Jaume" uniqKey="Torres J" first="Jaume" last="Torres">Jaume Torres</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore. Electronic address: jtorres@ntu.edu.sg.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Biochimica et biophysica acta. Biomembranes</title>
<idno type="ISSN">0005-2736</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term>
<term>Micelles</term>
<term>Models, Molecular</term>
<term>Nuclear Magnetic Resonance, Biomolecular</term>
<term>Phosphatidylglycerols (chemistry)</term>
<term>Protein Conformation</term>
<term>SARS Virus (chemistry)</term>
<term>Structure-Activity Relationship</term>
<term>Viral Envelope Proteins (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Conformation des protéines</term>
<term>Micelles</term>
<term>Modèles moléculaires</term>
<term>Phosphatidylglycérol ()</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Relation structure-activité</term>
<term>Résonance magnétique nucléaire biomoléculaire</term>
<term>Virus du SRAS ()</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Phosphatidylglycerols</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Micelles</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term>
<term>Models, Molecular</term>
<term>Nuclear Magnetic Resonance, Biomolecular</term>
<term>Protein Conformation</term>
<term>Structure-Activity Relationship</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Conformation des protéines</term>
<term>Micelles</term>
<term>Modèles moléculaires</term>
<term>Phosphatidylglycérol</term>
<term>Protéines de l'enveloppe virale</term>
<term>Relation structure-activité</term>
<term>Résonance magnétique nucléaire biomoléculaire</term>
<term>Virus du SRAS</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Coronaviruses (CoV) cause common colds in humans, but are also responsible for the recent Severe Acute, and Middle East, respiratory syndromes (SARS and MERS, respectively). A promising approach for prevention are live attenuated vaccines (LAVs), some of which target the envelope (E) protein, which is a small membrane protein that forms ion channels. Unfortunately, detailed structural information is still limited for SARS-CoV E, and non-existent for other CoV E proteins. Herein, we report a structural model of a SARS-CoV E construct in LMPG micelles with, for the first time, unequivocal intermolecular NOEs. The model corresponding to the detergent-embedded region is consistent with previously obtained orientational restraints obtained in lipid bilayers and in vivo escape mutants. The C-terminal domain is mostly α-helical, and extramembrane intermolecular NOEs suggest interactions that may affect the TM channel conformation.</div>
</front>
</TEI>
<affiliations><list><country><li>Singapour</li>
</country>
</list>
<tree><country name="Singapour"><noRegion><name sortKey="Surya, Wahyu" sort="Surya, Wahyu" uniqKey="Surya W" first="Wahyu" last="Surya">Wahyu Surya</name>
</noRegion>
<name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name>
<name sortKey="Torres, Jaume" sort="Torres, Jaume" uniqKey="Torres J" first="Jaume" last="Torres">Jaume Torres</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C34 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000C34 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:29474890
   |texte=   Structural model of the SARS coronavirus E channel in LMPG micelles.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:29474890" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

Structural model of the SARS coronavirus E channel in LMPG micelles.

Structural model of the SARS coronavirus E channel in LMPG micelles.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.