Recombinant scFv antibodies against E protein and N protein of severe acute respiratory syndrome virus.
Identifieur interne : 005167 ( Main/Curation ); précédent : 005166; suivant : 005168Recombinant scFv antibodies against E protein and N protein of severe acute respiratory syndrome virus.
Auteurs : Hui Liu [République populaire de Chine] ; Yan-Li Ding ; Wei Han ; Mei-Yun Liu ; Rui-Yang Tian ; Sheng-Li Yang ; Yi GongSource :
- Acta biochimica et biophysica Sinica [ 1672-9145 ] ; 2004.
Descripteurs français
- KwdFr :
- Affinité des anticorps, Animaux, Anticorps antiviraux (génétique), Anticorps antiviraux (métabolisme), Banque de peptides, Cinétique, Données de séquences moléculaires, Fragments d'immunoglobuline (génétique), Fragments d'immunoglobuline (métabolisme), Produits du gène env (immunologie), Protéines nucléocapside (immunologie), Souris, Séquence d'acides aminés, Techniques in vitro, Virus du SRAS (immunologie).
- MESH :
- génétique : Anticorps antiviraux, Fragments d'immunoglobuline.
- immunologie : Produits du gène env, Protéines nucléocapside, Virus du SRAS.
- métabolisme : Anticorps antiviraux, Fragments d'immunoglobuline.
- Affinité des anticorps, Animaux, Banque de peptides, Cinétique, Données de séquences moléculaires, Souris, Séquence d'acides aminés, Techniques in vitro.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (genetics), Antibodies, Viral (metabolism), Antibody Affinity, Gene Products, env (immunology), Immunoglobulin Fragments (genetics), Immunoglobulin Fragments (metabolism), In Vitro Techniques, Kinetics, Mice, Molecular Sequence Data, Nucleocapsid Proteins (immunology), Peptide Library, SARS Virus (immunology).
- MESH :
- chemical , genetics : Antibodies, Viral, Immunoglobulin Fragments.
- chemical , immunology : Gene Products, env, Nucleocapsid Proteins.
- chemical , metabolism : Antibodies, Viral, Immunoglobulin Fragments.
- immunology : SARS Virus.
- Amino Acid Sequence, Animals, Antibody Affinity, In Vitro Techniques, Kinetics, Mice, Molecular Sequence Data, Peptide Library.
Abstract
Three single chain antibodies (scFv) against the proteins of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated by phage display from an scFv antibody library. Bio-panning was carried out against immobilized purified envelope (E) and nucleocapsid (N) proteins of SARS-CoV. Their binding activity and specificity to E or N protein of SARS-CoV were characterized by phage-ELISA. Two of them, B10 and C20, could recognize non-overlapping epitopes of the E protein according to the two-site binding test result. Clone A17 could recognize N protein. The sequence of the epitope or overlapping epitope of scFv antibody A17 was PTDSTDNNQNGGRNGARPKQRRPQ. The affinity (equilibrium dissociation constant, K(d)) of SARS-CoV E protein was 5.7 x 10(-8) M for B10 and 8.9 x 10(-8) M for C20. The affinity of A17 for N protein was 2.1 x 10(-6) M. All three scFv antibodies were purified with affinity chromatography and determined by Western blot.
DOI: 10.1093/abbs/36.8.541
PubMed: 15295646
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last="Ding">Yan-Li Ding</name></author><author><name sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name></author><author><name sortKey="Liu, Mei Yun" sort="Liu, Mei Yun" uniqKey="Liu M" first="Mei-Yun" last="Liu">Mei-Yun Liu</name></author><author><name sortKey="Tian, Rui Yang" sort="Tian, Rui Yang" uniqKey="Tian R" first="Rui-Yang" last="Tian">Rui-Yang Tian</name></author><author><name sortKey="Yang, Sheng Li" sort="Yang, Sheng Li" uniqKey="Yang S" first="Sheng-Li" last="Yang">Sheng-Li Yang</name></author><author><name sortKey="Gong, Yi" sort="Gong, Yi" uniqKey="Gong Y" first="Yi" last="Gong">Yi Gong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15295646</idno><idno type="pmid">15295646</idno><idno type="doi">10.1093/abbs/36.8.541</idno><idno type="wicri:Area/PubMed/Corpus">002C34</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" 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Research Center of Biotechnology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200233, China. huiliu@srcb.ac.cn</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai Research Center of Biotechnology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200233</wicri:regionArea><wicri:noRegion>Shanghai 200233</wicri:noRegion></affiliation></author><author><name sortKey="Ding, Yan Li" sort="Ding, Yan Li" uniqKey="Ding Y" first="Yan-Li" last="Ding">Yan-Li Ding</name></author><author><name sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name></author><author><name sortKey="Liu, Mei Yun" sort="Liu, Mei Yun" uniqKey="Liu M" first="Mei-Yun" last="Liu">Mei-Yun Liu</name></author><author><name sortKey="Tian, Rui Yang" sort="Tian, Rui Yang" uniqKey="Tian R" first="Rui-Yang" last="Tian">Rui-Yang Tian</name></author><author><name sortKey="Yang, Sheng Li" sort="Yang, Sheng Li" uniqKey="Yang S" first="Sheng-Li" last="Yang">Sheng-Li Yang</name></author><author><name sortKey="Gong, Yi" sort="Gong, Yi" uniqKey="Gong Y" first="Yi" last="Gong">Yi Gong</name></author></analytic><series><title level="j">Acta biochimica et biophysica Sinica</title><idno type="ISSN">1672-9145</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Viral (genetics)</term><term>Antibodies, Viral (metabolism)</term><term>Antibody Affinity</term><term>Gene Products, env (immunology)</term><term>Immunoglobulin Fragments (genetics)</term><term>Immunoglobulin Fragments (metabolism)</term><term>In Vitro Techniques</term><term>Kinetics</term><term>Mice</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (immunology)</term><term>Peptide Library</term><term>SARS Virus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Affinité des anticorps</term><term>Animaux</term><term>Anticorps antiviraux (génétique)</term><term>Anticorps antiviraux (métabolisme)</term><term>Banque de peptides</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Fragments d'immunoglobuline (génétique)</term><term>Fragments d'immunoglobuline (métabolisme)</term><term>Produits du gène env (immunologie)</term><term>Protéines nucléocapside (immunologie)</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Techniques in vitro</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin Fragments</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Gene Products, env</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin Fragments</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Anticorps antiviraux</term><term>Fragments d'immunoglobuline</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Produits du gène env</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anticorps antiviraux</term><term>Fragments d'immunoglobuline</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibody Affinity</term><term>In Vitro Techniques</term><term>Kinetics</term><term>Mice</term><term>Molecular Sequence Data</term><term>Peptide Library</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Affinité des anticorps</term><term>Animaux</term><term>Banque de peptides</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Techniques in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three single chain antibodies (scFv) against the proteins of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated by phage display from an scFv antibody library. Bio-panning was carried out against immobilized purified envelope (E) and nucleocapsid (N) proteins of SARS-CoV. Their binding activity and specificity to E or N protein of SARS-CoV were characterized by phage-ELISA. Two of them, B10 and C20, could recognize non-overlapping epitopes of the E protein according to the two-site binding test result. Clone A17 could recognize N protein. The sequence of the epitope or overlapping epitope of scFv antibody A17 was PTDSTDNNQNGGRNGARPKQRRPQ. The affinity (equilibrium dissociation constant, K(d)) of SARS-CoV E protein was 5.7 x 10(-8) M for B10 and 8.9 x 10(-8) M for C20. The affinity of A17 for N protein was 2.1 x 10(-6) M. All three scFv antibodies were purified with affinity chromatography and determined by Western blot.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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