Ultrastructural characterization of SARS coronavirus.
Identifieur interne : 004F92 ( Main/Curation ); précédent : 004F91; suivant : 004F93Ultrastructural characterization of SARS coronavirus.
Auteurs : Cynthia S. Goldsmith [États-Unis] ; Kathleen M. Tatti ; Thomas G. Ksiazek ; Pierre E. Rollin ; James A. Comer ; William W. Lee ; Paul A. Rota ; Bettina Bankamp ; William J. Bellini ; Sherif R. ZakiSource :
- Emerging infectious diseases [ 1080-6040 ] ; 2004.
Descripteurs français
- KwdFr :
- ARN viral (génétique), ARN viral (métabolisme), Animaux, Assemblage viral, Cellules Vero, Corps d'inclusion viraux (ultrastructure), Humains, Hybridation in situ, Liquide de lavage bronchoalvéolaire (virologie), Microscopie immunoélectronique, Microscopie électronique, Protéines virales (métabolisme), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Virus du SRAS (physiologie), Virus du SRAS (ultrastructure).
- MESH :
- génétique : ARN viral, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- métabolisme : ARN viral, Protéines virales.
- physiologie : Virus du SRAS.
- virologie : Liquide de lavage bronchoalvéolaire, Syndrome respiratoire aigu sévère.
- ultrastructure : Animaux, Assemblage viral, Cellules Vero, Corps d'inclusion viraux, Humains, Hybridation in situ, Microscopie immunoélectronique, Microscopie électronique, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Bronchoalveolar Lavage Fluid (virology), Chlorocebus aethiops, Humans, In Situ Hybridization, Inclusion Bodies, Viral (ultrastructure), Microscopy, Electron, Microscopy, Immunoelectron, RNA, Viral (genetics), RNA, Viral (metabolism), SARS Virus (genetics), SARS Virus (isolation & purification), SARS Virus (physiology), SARS Virus (ultrastructure), Severe Acute Respiratory Syndrome (virology), Vero Cells, Viral Proteins (metabolism), Virus Assembly.
- MESH :
- chemical , genetics : RNA, Viral.
- chemical , metabolism : RNA, Viral, Viral Proteins.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- physiology : SARS Virus.
- ultrastructure : Inclusion Bodies, Viral, SARS Virus.
- virology : Bronchoalveolar Lavage Fluid, Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Humans, In Situ Hybridization, Microscopy, Electron, Microscopy, Immunoelectron, Vero Cells, Virus Assembly.
Abstract
Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations.
DOI: 10.3201/eid1002.030913
PubMed: 15030705
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Goldsmith</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. cgoldsmith@cdc.gov</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333</wicri:regionArea><wicri:noRegion>Georgia 30333</wicri:noRegion></affiliation></author><author><name sortKey="Tatti, Kathleen M" sort="Tatti, Kathleen M" uniqKey="Tatti K" first="Kathleen M" last="Tatti">Kathleen M. Tatti</name></author><author><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G" last="Ksiazek">Thomas G. 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Zaki</name></author></analytic><series><title level="j">Emerging infectious diseases</title><idno type="ISSN">1080-6040</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bronchoalveolar Lavage Fluid (virology)</term><term>Chlorocebus aethiops</term><term>Humans</term><term>In Situ Hybridization</term><term>Inclusion Bodies, Viral (ultrastructure)</term><term>Microscopy, Electron</term><term>Microscopy, Immunoelectron</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (physiology)</term><term>SARS Virus (ultrastructure)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Vero Cells</term><term>Viral Proteins (metabolism)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Assemblage viral</term><term>Cellules Vero</term><term>Corps d'inclusion viraux (ultrastructure)</term><term>Humains</term><term>Hybridation in situ</term><term>Liquide de lavage bronchoalvéolaire (virologie)</term><term>Microscopie immunoélectronique</term><term>Microscopie électronique</term><term>Protéines virales (métabolisme)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (physiologie)</term><term>Virus du SRAS (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Inclusion Bodies, Viral</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Liquide de lavage bronchoalvéolaire</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Bronchoalveolar Lavage Fluid</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>In Situ Hybridization</term><term>Microscopy, Electron</term><term>Microscopy, Immunoelectron</term><term>Vero Cells</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Cellules Vero</term><term>Corps d'inclusion viraux</term><term>Humains</term><term>Hybridation in situ</term><term>Microscopie immunoélectronique</term><term>Microscopie électronique</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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