[Cloning, purification, and antigenic characterization of three recombinant fragments derived from SARS-CoV S1 domain].
Identifieur interne : 004549 ( Main/Curation ); précédent : 004548; suivant : 004550[Cloning, purification, and antigenic characterization of three recombinant fragments derived from SARS-CoV S1 domain].
Auteurs : Ya-Bo Mei [République populaire de Chine] ; Zhi-Yong Liao ; Ya-Di Wang ; Li-Ya Zhang ; Hua Xu ; Kwok-Yung Yuen ; Xiao-Yan CheSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2005.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (sang), Antigènes de surface (génétique), Antigènes de surface (immunologie), Antigènes de surface (métabolisme), Humains, Immunoglobuline G (sang), Immunoglobuline M (sang), Plasmides (génétique), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Protéines recombinantes (immunologie), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Réaction de polymérisation en chaîne, Syndrome respiratoire aigu sévère (sang), Syndrome respiratoire aigu sévère (virologie), Technique de Western, Test ELISA, Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (métabolisme), Électrophorèse sur gel de polyacrylamide.
- MESH :
- génétique : Antigènes de surface, Plasmides, Protéines de l'enveloppe virale, Virus du SRAS.
- immunologie : Antigènes de surface, Protéines de l'enveloppe virale, Protéines recombinantes, Virus du SRAS.
- isolement et purification : Protéines recombinantes.
- métabolisme : Antigènes de surface, Protéines de l'enveloppe virale, Protéines recombinantes, Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline G, Immunoglobuline M, Syndrome respiratoire aigu sévère.
- virologie : Syndrome respiratoire aigu sévère.
- Humains, Réaction de polymérisation en chaîne, Technique de Western, Test ELISA, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Antigens, Surface (genetics), Antigens, Surface (immunology), Antigens, Surface (metabolism), Blotting, Western, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin G (blood), Immunoglobulin M (blood), Plasmids (genetics), Polymerase Chain Reaction, Recombinant Proteins (immunology), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (virology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G, Immunoglobulin M.
- chemical , genetics : Antigens, Surface, Viral Envelope Proteins.
- chemical , immunology : Antigens, Surface, Recombinant Proteins, Viral Envelope Proteins.
- chemical , isolation & purification : Recombinant Proteins.
- chemical , metabolism : Antigens, Surface, Recombinant Proteins, Viral Envelope Proteins.
- blood : Severe Acute Respiratory Syndrome.
- genetics : Plasmids, SARS Virus.
- immunology : SARS Virus.
- metabolism : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Blotting, Western, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Humans, Polymerase Chain Reaction.
Abstract
The present study aimed to clone and express three fragments of genomic RNA derived from SARS associated coronavirus (SARS-CoV) S1 domain and to study its immunogenicity.
PubMed: 16261215
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pubmed:16261215Le document en format XML
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<author><name sortKey="Mei, Ya Bo" sort="Mei, Ya Bo" uniqKey="Mei Y" first="Ya-Bo" last="Mei">Ya-Bo Mei</name>
<affiliation wicri:level="1"><nlm:affiliation>Central Laboraory, Zhujiang Hospital, The First Military Medical University, Guangzhou 510282, China.</nlm:affiliation>
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<author><name sortKey="Liao, Zhi Yong" sort="Liao, Zhi Yong" uniqKey="Liao Z" first="Zhi-Yong" last="Liao">Zhi-Yong Liao</name>
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<author><name sortKey="Wang, Ya Di" sort="Wang, Ya Di" uniqKey="Wang Y" first="Ya-Di" last="Wang">Ya-Di Wang</name>
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<author><name sortKey="Zhang, Li Ya" sort="Zhang, Li Ya" uniqKey="Zhang L" first="Li-Ya" last="Zhang">Li-Ya Zhang</name>
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<author><name sortKey="Xu, Hua" sort="Xu, Hua" uniqKey="Xu H" first="Hua" last="Xu">Hua Xu</name>
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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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<author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term>
<term>Antigens, Surface (genetics)</term>
<term>Antigens, Surface (immunology)</term>
<term>Antigens, Surface (metabolism)</term>
<term>Blotting, Western</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Immunoglobulin G (blood)</term>
<term>Immunoglobulin M (blood)</term>
<term>Plasmids (genetics)</term>
<term>Polymerase Chain Reaction</term>
<term>Recombinant Proteins (immunology)</term>
<term>Recombinant Proteins (isolation & purification)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (immunology)</term>
<term>SARS Virus (metabolism)</term>
<term>Severe Acute Respiratory Syndrome (blood)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
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<term>Antigènes de surface (génétique)</term>
<term>Antigènes de surface (immunologie)</term>
<term>Antigènes de surface (métabolisme)</term>
<term>Humains</term>
<term>Immunoglobuline G (sang)</term>
<term>Immunoglobuline M (sang)</term>
<term>Plasmides (génétique)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Protéines recombinantes (isolement et purification)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Syndrome respiratoire aigu sévère (sang)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (immunologie)</term>
<term>Virus du SRAS (métabolisme)</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
<term>Immunoglobulin G</term>
<term>Immunoglobulin M</term>
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<term>Viral Envelope Proteins</term>
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<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Surface</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes de surface</term>
<term>Plasmides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes de surface</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes de surface</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Immunoglobuline G</term>
<term>Immunoglobuline M</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Humains</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
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<front><div type="abstract" xml:lang="en">The present study aimed to clone and express three fragments of genomic RNA derived from SARS associated coronavirus (SARS-CoV) S1 domain and to study its immunogenicity.</div>
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