Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
Identifieur interne : 003E61 ( Main/Curation ); précédent : 003E60; suivant : 003E62Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
Auteurs : Thai-Yen Ling [Taïwan] ; Ming-Der Kuo ; Chung-Leung Li ; Alice L. Yu ; Yen-Hua Huang ; Tsai-Jung Wu ; You-Chin Lin ; Shu-Hwa Chen ; John YuSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Broxuridine, Cellules cultivées, Cellules souches (cytologie), Cellules souches (métabolisme), Cellules souches (virologie), Cellules épithéliales (cytologie), Cellules épithéliales (métabolisme), Cellules épithéliales (virologie), Différenciation cellulaire, Facteur de transcription Oct-3 (métabolisme), Marqueurs biologiques, Microscopie électronique à transmission, Poumon (cytologie), Poumon (virologie), Régulation de l'expression des gènes, Souris, Souris de lignée ICR, Virus du SRAS (physiologie).
- MESH :
- cytologie : Cellules souches, Cellules épithéliales, Poumon.
- métabolisme : Cellules souches, Cellules épithéliales, Facteur de transcription Oct-3.
- physiologie : Virus du SRAS.
- virologie : Cellules souches, Cellules épithéliales, Poumon.
- Animaux, Broxuridine, Cellules cultivées, Différenciation cellulaire, Marqueurs biologiques, Microscopie électronique à transmission, Régulation de l'expression des gènes, Souris, Souris de lignée ICR.
English descriptors
- KwdEn :
- Animals, Biomarkers, Bromodeoxyuridine, Cell Differentiation, Cells, Cultured, Epithelial Cells (cytology), Epithelial Cells (metabolism), Epithelial Cells (virology), Gene Expression Regulation, Lung (cytology), Lung (virology), Mice, Mice, Inbred ICR, Microscopy, Electron, Transmission, Octamer Transcription Factor-3 (metabolism), SARS Virus (physiology), Stem Cells (cytology), Stem Cells (metabolism), Stem Cells (virology).
- MESH :
- chemical , metabolism : Octamer Transcription Factor-3.
- chemical : Biomarkers, Bromodeoxyuridine.
- cytology : Epithelial Cells, Lung, Stem Cells.
- metabolism : Epithelial Cells, Stem Cells.
- physiology : SARS Virus.
- virology : Epithelial Cells, Lung, Stem Cells.
- Animals, Cell Differentiation, Cells, Cultured, Gene Expression Regulation, Mice, Mice, Inbred ICR, Microscopy, Electron, Transmission.
Abstract
In this study, we report a serum-free culture system for primary neonatal pulmonary cells that can support the growth of octamer-binding transcription factor 4+ (Oct-4+) epithelial colonies with a surrounding mesenchymal stroma. In addition to Oct-4, these cells also express other stem cell markers such as stage-specific embryonic antigen 1 (SSEA-1), stem cell antigen 1 (Sca-1), and Clara cell secretion protein (CCSP) but not c-Kit, CD34, and p63, indicating that they represent a subpopulation of Clara cells that have been implicated as lung stem/progenitor cells in lung injury models. These colony cells can be kept for weeks in primary cultures and undergo terminal differentiation to alveolar type-2- and type-1-like pneumocytes sequentially when removed from the stroma. In addition, we have demonstrated the presence of Oct-4+ long-term BrdU label-retaining cells at the bronchoalveolar junction of neonatal lung, providing a link between the Oct-4+ cells in vivo and in vitro and strengthening their identity as putative neonatal lung stem/progenitor cells. Lastly, these Oct-4+ epithelial colony cells, which also express angiotensin-converting enzyme 2, are the target cells for severe acute respiratory syndrome coronavirus infection in primary cultures and support active virus replication leading to their own destruction. These observations imply the possible involvement of lung stem/progenitor cells, in addition to pneumocytes, in severe acute respiratory syndrome coronavirus infection, accounting for the continued deterioration of lung tissues and apparent loss of capacity for lung repair.
DOI: 10.1073/pnas.0510232103
PubMed: 16772384
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pubmed:16772384Le document en format XML
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Yu</name></author><author><name sortKey="Huang, Yen Hua" sort="Huang, Yen Hua" uniqKey="Huang Y" first="Yen-Hua" last="Huang">Yen-Hua Huang</name></author><author><name sortKey="Wu, Tsai Jung" sort="Wu, Tsai Jung" uniqKey="Wu T" first="Tsai-Jung" last="Wu">Tsai-Jung Wu</name></author><author><name sortKey="Lin, You Chin" sort="Lin, You Chin" uniqKey="Lin Y" first="You-Chin" last="Lin">You-Chin Lin</name></author><author><name sortKey="Chen, Shu Hwa" sort="Chen, Shu Hwa" uniqKey="Chen S" first="Shu-Hwa" last="Chen">Shu-Hwa Chen</name></author><author><name sortKey="Yu, John" sort="Yu, John" uniqKey="Yu J" first="John" last="Yu">John Yu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16772384</idno><idno type="pmid">16772384</idno><idno type="doi">10.1073/pnas.0510232103</idno><idno type="wicri:Area/PubMed/Corpus">002203</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002203</idno><idno type="wicri:Area/PubMed/Curation">002203</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002203</idno><idno type="wicri:Area/PubMed/Checkpoint">002154</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002154</idno><idno type="wicri:Area/Ncbi/Merge">001535</idno><idno type="wicri:Area/Ncbi/Curation">001535</idno><idno type="wicri:Area/Ncbi/Checkpoint">001535</idno><idno type="wicri:doubleKey">0027-8424:2006:Ling T:identification:of:pulmonary</idno><idno type="wicri:Area/Main/Merge">004050</idno><idno type="wicri:Area/Main/Curation">003E61</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.</title><author><name sortKey="Ling, Thai Yen" sort="Ling, Thai Yen" uniqKey="Ling T" first="Thai-Yen" last="Ling">Thai-Yen Ling</name><affiliation wicri:level="1"><nlm:affiliation>Stem Cell Program, Cellular and Molecular Medicine Division, Genomics Research Center, Academia Sinica, Taipei 115, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Stem Cell Program, Cellular and Molecular Medicine Division, Genomics Research Center, Academia Sinica, Taipei 115</wicri:regionArea><wicri:noRegion>Taipei 115</wicri:noRegion></affiliation></author><author><name sortKey="Kuo, Ming Der" sort="Kuo, Ming Der" uniqKey="Kuo M" first="Ming-Der" last="Kuo">Ming-Der Kuo</name></author><author><name sortKey="Li, Chung Leung" sort="Li, Chung Leung" uniqKey="Li C" first="Chung-Leung" last="Li">Chung-Leung Li</name></author><author><name sortKey="Yu, Alice L" sort="Yu, Alice L" uniqKey="Yu A" first="Alice L" last="Yu">Alice L. Yu</name></author><author><name sortKey="Huang, Yen Hua" sort="Huang, Yen Hua" uniqKey="Huang Y" first="Yen-Hua" last="Huang">Yen-Hua Huang</name></author><author><name sortKey="Wu, Tsai Jung" sort="Wu, Tsai Jung" uniqKey="Wu T" first="Tsai-Jung" last="Wu">Tsai-Jung Wu</name></author><author><name sortKey="Lin, You Chin" sort="Lin, You Chin" uniqKey="Lin Y" first="You-Chin" last="Lin">You-Chin Lin</name></author><author><name sortKey="Chen, Shu Hwa" sort="Chen, Shu Hwa" uniqKey="Chen S" first="Shu-Hwa" last="Chen">Shu-Hwa Chen</name></author><author><name sortKey="Yu, John" sort="Yu, John" uniqKey="Yu J" first="John" last="Yu">John Yu</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biomarkers</term><term>Bromodeoxyuridine</term><term>Cell Differentiation</term><term>Cells, Cultured</term><term>Epithelial Cells (cytology)</term><term>Epithelial Cells (metabolism)</term><term>Epithelial Cells (virology)</term><term>Gene Expression Regulation</term><term>Lung (cytology)</term><term>Lung (virology)</term><term>Mice</term><term>Mice, Inbred ICR</term><term>Microscopy, Electron, Transmission</term><term>Octamer Transcription Factor-3 (metabolism)</term><term>SARS Virus (physiology)</term><term>Stem Cells (cytology)</term><term>Stem Cells (metabolism)</term><term>Stem Cells (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Broxuridine</term><term>Cellules cultivées</term><term>Cellules souches (cytologie)</term><term>Cellules souches (métabolisme)</term><term>Cellules souches (virologie)</term><term>Cellules épithéliales (cytologie)</term><term>Cellules épithéliales (métabolisme)</term><term>Cellules épithéliales (virologie)</term><term>Différenciation cellulaire</term><term>Facteur de transcription Oct-3 (métabolisme)</term><term>Marqueurs biologiques</term><term>Microscopie électronique à transmission</term><term>Poumon (cytologie)</term><term>Poumon (virologie)</term><term>Régulation de l'expression des gènes</term><term>Souris</term><term>Souris de lignée ICR</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Octamer Transcription Factor-3</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biomarkers</term><term>Bromodeoxyuridine</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules souches</term><term>Cellules épithéliales</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Epithelial Cells</term><term>Lung</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules souches</term><term>Cellules épithéliales</term><term>Facteur de transcription Oct-3</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules souches</term><term>Cellules épithéliales</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term><term>Lung</term><term>Stem Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Differentiation</term><term>Cells, Cultured</term><term>Gene Expression Regulation</term><term>Mice</term><term>Mice, Inbred ICR</term><term>Microscopy, Electron, Transmission</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Broxuridine</term><term>Cellules cultivées</term><term>Différenciation cellulaire</term><term>Marqueurs biologiques</term><term>Microscopie électronique à transmission</term><term>Régulation de l'expression des gènes</term><term>Souris</term><term>Souris de lignée ICR</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, we report a serum-free culture system for primary neonatal pulmonary cells that can support the growth of octamer-binding transcription factor 4+ (Oct-4+) epithelial colonies with a surrounding mesenchymal stroma. In addition to Oct-4, these cells also express other stem cell markers such as stage-specific embryonic antigen 1 (SSEA-1), stem cell antigen 1 (Sca-1), and Clara cell secretion protein (CCSP) but not c-Kit, CD34, and p63, indicating that they represent a subpopulation of Clara cells that have been implicated as lung stem/progenitor cells in lung injury models. These colony cells can be kept for weeks in primary cultures and undergo terminal differentiation to alveolar type-2- and type-1-like pneumocytes sequentially when removed from the stroma. In addition, we have demonstrated the presence of Oct-4+ long-term BrdU label-retaining cells at the bronchoalveolar junction of neonatal lung, providing a link between the Oct-4+ cells in vivo and in vitro and strengthening their identity as putative neonatal lung stem/progenitor cells. Lastly, these Oct-4+ epithelial colony cells, which also express angiotensin-converting enzyme 2, are the target cells for severe acute respiratory syndrome coronavirus infection in primary cultures and support active virus replication leading to their own destruction. These observations imply the possible involvement of lung stem/progenitor cells, in addition to pneumocytes, in severe acute respiratory syndrome coronavirus infection, accounting for the continued deterioration of lung tissues and apparent loss of capacity for lung repair.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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