Label-free, electrical detection of the SARS virus N-protein with nanowire biosensors utilizing antibody mimics as capture probes.
Identifieur interne : 002A84 ( Main/Curation ); précédent : 002A83; suivant : 002A85Label-free, electrical detection of the SARS virus N-protein with nanowire biosensors utilizing antibody mimics as capture probes.
Auteurs : Fumiaki N. Ishikawa [États-Unis] ; Hsiao-Kang Chang ; Marco Curreli ; Hsiang-I Liao ; C Anders Olson ; Po-Chiang Chen ; Rui Zhang ; Richard W. Roberts ; Ren Sun ; Richard J. Cote ; Mark E. Thompson ; Chongwu ZhouSource :
- ACS nano [ 1936-086X ] ; 2009.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (), Coloration et marquage, Conception d'appareillage, Conformation moléculaire, Fibronectines (immunologie), Indium (), Matériaux biomimétiques (), Nanostructures (), Nanostructures (ultrastructure), Nanotechnologie (instrumentation), Propriétés de surface, Structures macromoléculaires (), Taille de particule, Techniques de biocapteur (instrumentation), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification), Électrochimie (instrumentation).
- MESH :
- immunologie : Fibronectines, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Anticorps antiviraux, Coloration et marquage, Conception d'appareillage, Conformation moléculaire, Indium, Matériaux biomimétiques, Nanostructures, Nanotechnologie, Propriétés de surface, Structures macromoléculaires, Taille de particule, Techniques de biocapteur, Électrochimie.
English descriptors
- KwdEn :
- Antibodies, Viral (chemistry), Biomimetic Materials (chemistry), Biosensing Techniques (instrumentation), Electrochemistry (instrumentation), Equipment Design, Fibronectins (immunology), Indium (chemistry), Macromolecular Substances (chemistry), Molecular Conformation, Nanostructures (chemistry), Nanostructures (ultrastructure), Nanotechnology (instrumentation), Particle Size, SARS Virus (immunology), SARS Virus (isolation & purification), Staining and Labeling, Surface Properties.
- MESH :
- chemical , chemistry : Antibodies, Viral, Indium, Macromolecular Substances.
- chemistry : Biomimetic Materials, Nanostructures.
- chemical , immunology : Fibronectins, SARS Virus.
- instrumentation : Biosensing Techniques, Electrochemistry, Nanotechnology.
- isolation & purification : SARS Virus.
- ultrastructure : Nanostructures.
- Equipment Design, Molecular Conformation, Particle Size, Staining and Labeling, Surface Properties.
Abstract
Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In(2)O(3) nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 microM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.
DOI: 10.1021/nn900086c
PubMed: 19422193
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pubmed:19422193Le document en format XML
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Ishikawa</name><affiliation wicri:level="4"><nlm:affiliation>Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Chang, Hsiao Kang" sort="Chang, Hsiao Kang" uniqKey="Chang H" first="Hsiao-Kang" last="Chang">Hsiao-Kang Chang</name></author><author><name sortKey="Curreli, Marco" sort="Curreli, Marco" uniqKey="Curreli M" first="Marco" last="Curreli">Marco Curreli</name></author><author><name sortKey="Liao, Hsiang I" sort="Liao, Hsiang I" uniqKey="Liao H" first="Hsiang-I" last="Liao">Hsiang-I Liao</name></author><author><name sortKey="Olson, C Anders" sort="Olson, C Anders" uniqKey="Olson C" first="C Anders" last="Olson">C Anders Olson</name></author><author><name sortKey="Chen, Po Chiang" sort="Chen, Po Chiang" uniqKey="Chen P" first="Po-Chiang" last="Chen">Po-Chiang Chen</name></author><author><name sortKey="Zhang, Rui" sort="Zhang, Rui" uniqKey="Zhang R" first="Rui" last="Zhang">Rui Zhang</name></author><author><name sortKey="Roberts, Richard W" sort="Roberts, Richard W" uniqKey="Roberts R" first="Richard W" last="Roberts">Richard W. Roberts</name></author><author><name sortKey="Sun, Ren" sort="Sun, Ren" uniqKey="Sun R" first="Ren" last="Sun">Ren Sun</name></author><author><name sortKey="Cote, Richard J" sort="Cote, Richard J" uniqKey="Cote R" first="Richard J" last="Cote">Richard J. Cote</name></author><author><name sortKey="Thompson, Mark E" sort="Thompson, Mark E" uniqKey="Thompson M" first="Mark E" last="Thompson">Mark E. 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Ishikawa</name><affiliation wicri:level="4"><nlm:affiliation>Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Chang, Hsiao Kang" sort="Chang, Hsiao Kang" uniqKey="Chang H" first="Hsiao-Kang" last="Chang">Hsiao-Kang Chang</name></author><author><name sortKey="Curreli, Marco" sort="Curreli, Marco" uniqKey="Curreli M" first="Marco" last="Curreli">Marco Curreli</name></author><author><name sortKey="Liao, Hsiang I" sort="Liao, Hsiang I" uniqKey="Liao H" first="Hsiang-I" last="Liao">Hsiang-I Liao</name></author><author><name sortKey="Olson, C Anders" sort="Olson, C Anders" uniqKey="Olson C" first="C Anders" last="Olson">C Anders Olson</name></author><author><name sortKey="Chen, Po Chiang" sort="Chen, Po Chiang" uniqKey="Chen P" first="Po-Chiang" last="Chen">Po-Chiang Chen</name></author><author><name sortKey="Zhang, Rui" sort="Zhang, Rui" uniqKey="Zhang R" first="Rui" last="Zhang">Rui Zhang</name></author><author><name sortKey="Roberts, Richard W" sort="Roberts, Richard W" uniqKey="Roberts R" first="Richard W" last="Roberts">Richard W. Roberts</name></author><author><name sortKey="Sun, Ren" sort="Sun, Ren" uniqKey="Sun R" first="Ren" last="Sun">Ren Sun</name></author><author><name sortKey="Cote, Richard J" sort="Cote, Richard J" uniqKey="Cote R" first="Richard J" last="Cote">Richard J. Cote</name></author><author><name sortKey="Thompson, Mark E" sort="Thompson, Mark E" uniqKey="Thompson M" first="Mark E" last="Thompson">Mark E. Thompson</name></author><author><name sortKey="Zhou, Chongwu" sort="Zhou, Chongwu" uniqKey="Zhou C" first="Chongwu" last="Zhou">Chongwu Zhou</name></author></analytic><series><title level="j">ACS nano</title><idno type="eISSN">1936-086X</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (chemistry)</term><term>Biomimetic Materials (chemistry)</term><term>Biosensing Techniques (instrumentation)</term><term>Electrochemistry (instrumentation)</term><term>Equipment Design</term><term>Fibronectins (immunology)</term><term>Indium (chemistry)</term><term>Macromolecular Substances (chemistry)</term><term>Molecular Conformation</term><term>Nanostructures (chemistry)</term><term>Nanostructures (ultrastructure)</term><term>Nanotechnology (instrumentation)</term><term>Particle Size</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Staining and Labeling</term><term>Surface Properties</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux ()</term><term>Coloration et marquage</term><term>Conception d'appareillage</term><term>Conformation moléculaire</term><term>Fibronectines (immunologie)</term><term>Indium ()</term><term>Matériaux biomimétiques ()</term><term>Nanostructures ()</term><term>Nanostructures (ultrastructure)</term><term>Nanotechnologie (instrumentation)</term><term>Propriétés de surface</term><term>Structures macromoléculaires ()</term><term>Taille de particule</term><term>Techniques de biocapteur (instrumentation)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term><term>Électrochimie (instrumentation)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies, Viral</term><term>Indium</term><term>Macromolecular Substances</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Biomimetic Materials</term><term>Nanostructures</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Fibronectines</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Fibronectins</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term><term>Electrochemistry</term><term>Nanotechnology</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanostructures</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Equipment Design</term><term>Molecular Conformation</term><term>Particle Size</term><term>Staining and Labeling</term><term>Surface Properties</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Anticorps antiviraux</term><term>Coloration et marquage</term><term>Conception d'appareillage</term><term>Conformation moléculaire</term><term>Indium</term><term>Matériaux biomimétiques</term><term>Nanostructures</term><term>Nanotechnologie</term><term>Propriétés de surface</term><term>Structures macromoléculaires</term><term>Taille de particule</term><term>Techniques de biocapteur</term><term>Électrochimie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In(2)O(3) nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 microM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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