Identification of Mammalian Orthoreovirus Type 3 in Italian Bats
Identifieur interne : 001A97 ( Main/Curation ); précédent : 001A96; suivant : 001A98Identification of Mammalian Orthoreovirus Type 3 in Italian Bats
Auteurs : D. Lelli [Italie] ; A. Moreno [Italie] ; A. Lavazza [Italie] ; M. Bresaola [Italie] ; E. Canelli [Italie] ; M. B. Boniotti [Italie] ; P. Cordioli [Italie]Source :
- Zoonoses and Public Health [ 1863-1959 ] ; 2013-02.
English descriptors
- Teeft :
- Accession, Amino acid residues, Asymptomatic infections, Blackwell verlag gmbh zoonoses, Carcassa, Cell culture, Cell culture supernatants, Cell cultures, Cytopathic effect, Dermody, Different serotypes, Faecal, Faecal samples, Fields virology, Fruit bats, Genus orthoreovirus, Gmbh, Important reservoir hosts, Infectious diseases, Intestinal proteases, Italian bats, Italian microbats, Italian strains, Kuhlii, Lelli, Mammalian, Mammalian orthoreovirus, Mammalian orthoreovirus type, Mammalian reovirus, Mammalian reoviruses, Maximum likelihood, Molecular analysis, Molecular characterization, Negative staining electron microscopy, Organ samples, Orthoreovirus, Orthoreoviruses, Palau virus, Phylogenetic, Phylogenetic analysis, Phylogenetic tree, Pipistrellus, Pipistrellus khulii, Positive samples, Primer, Primer position, Pteropine orthoreovirus, Public health, Reference strains, Rehabilitation centres, Reovirus, Reovirus type, Rhinolophus hipposideros, Sequence diversity, Serotypes, Sialic acid, Species barriers, Study reports, Tadarida teniotis, Tyler, Urban areas, Verlag, Vespertilio murinus, Viral, Viral serotype, Virol, Virological, Virological tests, Zoonosis.
Abstract
Summary This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested. In total, 112 carcasses of bats found dead, and 44 faecal samples were analysed. Nineteen viral strains were isolated by in vitro cell culture from faecal and tissue samples of different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros and Vespertilio murinus), and they were morphologically identified as reoviruses by negative staining electron microscopy observation. The definitive assignment of all isolates to MRV was confirmed by RT‐PCR assays targeting the L1 gene. Through a multiplex RT‐PCR assay targeting the S1 gene, we typed 15 of 19 isolates as MRV type 3. Partial L1 (416 bp) and complete S1 (1416 bp) sequences of the isolates were analysed and compared with those of reference strains obtained from GenBank, belonging to the three serotypes. Molecular analysis of the S1 gene revealed that the amino acid residues associated with neurotropism (198‐204NLAIRLP, 249I, 350D and 419E) were highly conserved among the Italian bat strains. These results suggest that potentially neurotropic MRV type 3 strains are widespread among Italian bats. Furthermore, the identification of MRV type 3 in bat species such as Pipistrellus Khulii, which is common in urban areas and known for its close contact with humans, underlines the need for vigilance.
Url:
DOI: 10.1111/zph.12001
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001737
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :001737
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000375
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :001B07
Links to Exploration step
ISTEX:8320737F6DF8342BB775EA8E2383EA43DF85DEC4Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of Mammalian Orthoreovirus Type 3 in Italian Bats</title><author><name sortKey="Lelli, D" sort="Lelli, D" uniqKey="Lelli D" first="D." last="Lelli">D. Lelli</name></author><author><name sortKey="Moreno, A" sort="Moreno, A" uniqKey="Moreno A" first="A." last="Moreno">A. Moreno</name></author><author><name sortKey="Lavazza, A" sort="Lavazza, A" uniqKey="Lavazza A" first="A." last="Lavazza">A. Lavazza</name></author><author><name sortKey="Bresaola, M" sort="Bresaola, M" uniqKey="Bresaola M" first="M." last="Bresaola">M. Bresaola</name></author><author><name sortKey="Canelli, E" sort="Canelli, E" uniqKey="Canelli E" first="E." last="Canelli">E. Canelli</name></author><author><name sortKey="Boniotti, M B" sort="Boniotti, M B" uniqKey="Boniotti M" first="M. B." last="Boniotti">M. B. Boniotti</name></author><author><name sortKey="Cordioli, P" sort="Cordioli, P" uniqKey="Cordioli P" first="P." last="Cordioli">P. Cordioli</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8320737F6DF8342BB775EA8E2383EA43DF85DEC4</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1111/zph.12001</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-2HCLLZQC-K/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001737</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001737</idno><idno type="wicri:Area/Istex/Curation">001737</idno><idno type="wicri:Area/Istex/Checkpoint">000375</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000375</idno><idno type="wicri:doubleKey">1863-1959:2013:Lelli D:identification:of:mammalian</idno><idno type="wicri:Area/Main/Merge">001B07</idno><idno type="wicri:Area/Main/Curation">001A97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Identification of Mammalian Orthoreovirus Type 3 in Italian Bats</title><author><name sortKey="Lelli, D" sort="Lelli, D" uniqKey="Lelli D" first="D." last="Lelli">D. Lelli</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, IZSLER, Brescia</wicri:regionArea><wicri:noRegion>Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Moreno, A" sort="Moreno, A" uniqKey="Moreno A" first="A." last="Moreno">A. Moreno</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, IZSLER, Brescia</wicri:regionArea><wicri:noRegion>Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Lavazza, A" sort="Lavazza, A" uniqKey="Lavazza A" first="A." last="Lavazza">A. Lavazza</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, IZSLER, Brescia</wicri:regionArea><wicri:noRegion>Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Bresaola, M" sort="Bresaola, M" uniqKey="Bresaola M" first="M." last="Bresaola">M. Bresaola</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, IZSLER, Brescia</wicri:regionArea><wicri:noRegion>Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Canelli, E" sort="Canelli, E" uniqKey="Canelli E" first="E." last="Canelli">E. Canelli</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, IZSLER, Brescia</wicri:regionArea><wicri:noRegion>Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Boniotti, M B" sort="Boniotti, M B" uniqKey="Boniotti M" first="M. B." last="Boniotti">M. B. Boniotti</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, IZSLER, Brescia</wicri:regionArea><wicri:noRegion>Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Cordioli, P" sort="Cordioli, P" uniqKey="Cordioli P" first="P." last="Cordioli">P. Cordioli</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, IZSLER, Brescia</wicri:regionArea><wicri:noRegion>Brescia</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Zoonoses and Public Health</title><title level="j" type="sub">Bats and Zoonoses</title><title level="j" type="alt">ZOONOSES AND PUBLIC HEALTH</title><idno type="ISSN">1863-1959</idno><idno type="eISSN">1863-2378</idno><imprint><biblScope unit="vol">60</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="84">84</biblScope><biblScope unit="page" to="92">92</biblScope><biblScope unit="page-count">9</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2013-02">2013-02</date></imprint><idno type="ISSN">1863-1959</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1863-1959</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Accession</term><term>Amino acid residues</term><term>Asymptomatic infections</term><term>Blackwell verlag gmbh zoonoses</term><term>Carcassa</term><term>Cell culture</term><term>Cell culture supernatants</term><term>Cell cultures</term><term>Cytopathic effect</term><term>Dermody</term><term>Different serotypes</term><term>Faecal</term><term>Faecal samples</term><term>Fields virology</term><term>Fruit bats</term><term>Genus orthoreovirus</term><term>Gmbh</term><term>Important reservoir hosts</term><term>Infectious diseases</term><term>Intestinal proteases</term><term>Italian bats</term><term>Italian microbats</term><term>Italian strains</term><term>Kuhlii</term><term>Lelli</term><term>Mammalian</term><term>Mammalian orthoreovirus</term><term>Mammalian orthoreovirus type</term><term>Mammalian reovirus</term><term>Mammalian reoviruses</term><term>Maximum likelihood</term><term>Molecular analysis</term><term>Molecular characterization</term><term>Negative staining electron microscopy</term><term>Organ samples</term><term>Orthoreovirus</term><term>Orthoreoviruses</term><term>Palau virus</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Phylogenetic tree</term><term>Pipistrellus</term><term>Pipistrellus khulii</term><term>Positive samples</term><term>Primer</term><term>Primer position</term><term>Pteropine orthoreovirus</term><term>Public health</term><term>Reference strains</term><term>Rehabilitation centres</term><term>Reovirus</term><term>Reovirus type</term><term>Rhinolophus hipposideros</term><term>Sequence diversity</term><term>Serotypes</term><term>Sialic acid</term><term>Species barriers</term><term>Study reports</term><term>Tadarida teniotis</term><term>Tyler</term><term>Urban areas</term><term>Verlag</term><term>Vespertilio murinus</term><term>Viral</term><term>Viral serotype</term><term>Virol</term><term>Virological</term><term>Virological tests</term><term>Zoonosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested. In total, 112 carcasses of bats found dead, and 44 faecal samples were analysed. Nineteen viral strains were isolated by in vitro cell culture from faecal and tissue samples of different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros and Vespertilio murinus), and they were morphologically identified as reoviruses by negative staining electron microscopy observation. The definitive assignment of all isolates to MRV was confirmed by RT‐PCR assays targeting the L1 gene. Through a multiplex RT‐PCR assay targeting the S1 gene, we typed 15 of 19 isolates as MRV type 3. Partial L1 (416 bp) and complete S1 (1416 bp) sequences of the isolates were analysed and compared with those of reference strains obtained from GenBank, belonging to the three serotypes. Molecular analysis of the S1 gene revealed that the amino acid residues associated with neurotropism (198‐204NLAIRLP, 249I, 350D and 419E) were highly conserved among the Italian bat strains. These results suggest that potentially neurotropic MRV type 3 strains are widespread among Italian bats. Furthermore, the identification of MRV type 3 in bat species such as Pipistrellus Khulii, which is common in urban areas and known for its close contact with humans, underlines the need for vigilance.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A97 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 001A97 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Curation
|type= RBID
|clé= ISTEX:8320737F6DF8342BB775EA8E2383EA43DF85DEC4
|texte= Identification of Mammalian Orthoreovirus Type 3 in Italian Bats
}}
| This area was generated with Dilib version V0.6.33. |  |