Evaluation of a recombinant nucleocapsid protein‐based assay for Anti‐SARS‐CoV IgG detection
Identifieur interne : 000768 ( Istex/Curation ); précédent : 000767; suivant : 000769Evaluation of a recombinant nucleocapsid protein‐based assay for Anti‐SARS‐CoV IgG detection
Auteurs : Paul K. S. Chan [République populaire de Chine, Hong Kong] ; Esther Y. M. Liu [République populaire de Chine] ; Danny T. M. Leung [République populaire de Chine] ; Jo L. K. Cheung [République populaire de Chine] ; C. H. Ma [République populaire de Chine] ; Frankie C. H. Tam [République populaire de Chine] ; Mamie Hui [République populaire de Chine] ; John S. Tam [République populaire de Chine] ; Pak Leong Lim [République populaire de Chine]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2005-02.
English descriptors
- Teeft :
- Antibody response, Assay, Chan, Chinese university, Clin, Clin diagn, Coronavirus, Coronavirus nucleocapsid protein, Early samples, Elisarstm, Enzyme immunoassay, Hong kong, Iggene, Immunoassay, Novel coronavirus, Nucleocapsid, Nucleocapsid protein, Peiris, Recombinant, Respiratory syndrome, Respiratory syndrome coronavirus, Sars, Sars patients, Serum samples, Syndrome, Wales hospital, Wang.
Abstract
A high throughput accurate assay for anti‐SARS‐CoV IgG detection is needed for large‐scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein‐based microtitre plate enzyme immunoassay, ELISARS™ is described. The results on 150 sera from SARS patients and 450 sera from non‐SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large‐scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti‐nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation. J. Med. Virol. 75:181–184, 2005. © 2004 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20254
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<term>Clin diagn</term>
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<term>Coronavirus nucleocapsid protein</term>
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<front><div type="abstract" xml:lang="en">A high throughput accurate assay for anti‐SARS‐CoV IgG detection is needed for large‐scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein‐based microtitre plate enzyme immunoassay, ELISARS™ is described. The results on 150 sera from SARS patients and 450 sera from non‐SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large‐scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti‐nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation. J. Med. Virol. 75:181–184, 2005. © 2004 Wiley‐Liss, Inc.</div>
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