Evaluation of a recombinant nucleocapsid protein‐based assay for Anti‐SARS‐CoV IgG detection
Identifieur interne : 000768 ( Istex/Curation ); précédent : 000767; suivant : 000769Evaluation of a recombinant nucleocapsid protein‐based assay for Anti‐SARS‐CoV IgG detection
Auteurs : Paul K. S. Chan [République populaire de Chine, Hong Kong] ; Esther Y. M. Liu [République populaire de Chine] ; Danny T. M. Leung [République populaire de Chine] ; Jo L. K. Cheung [République populaire de Chine] ; C. H. Ma [République populaire de Chine] ; Frankie C. H. Tam [République populaire de Chine] ; Mamie Hui [République populaire de Chine] ; John S. Tam [République populaire de Chine] ; Pak Leong Lim [République populaire de Chine]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2005-02.
English descriptors
- Teeft :
- Antibody response, Assay, Chan, Chinese university, Clin, Clin diagn, Coronavirus, Coronavirus nucleocapsid protein, Early samples, Elisarstm, Enzyme immunoassay, Hong kong, Iggene, Immunoassay, Novel coronavirus, Nucleocapsid, Nucleocapsid protein, Peiris, Recombinant, Respiratory syndrome, Respiratory syndrome coronavirus, Sars, Sars patients, Serum samples, Syndrome, Wales hospital, Wang.
Abstract
A high throughput accurate assay for anti‐SARS‐CoV IgG detection is needed for large‐scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein‐based microtitre plate enzyme immunoassay, ELISARS™ is described. The results on 150 sera from SARS patients and 450 sera from non‐SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large‐scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti‐nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation. J. Med. Virol. 75:181–184, 2005. © 2004 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20254
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Chan</name><affiliation wicri:level="1"><mods:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: paulkschan@cuhk.edu.hk</mods:affiliation><country wicri:rule="url">Hong Kong</country></affiliation><affiliation wicri:level="1"><mods:affiliation>Correspondence address: Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China.===</mods:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Correspondence address: Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y. 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Chan</name><affiliation wicri:level="1"><mods:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: paulkschan@cuhk.edu.hk</mods:affiliation><country wicri:rule="url">Hong Kong</country></affiliation><affiliation wicri:level="1"><mods:affiliation>Correspondence address: Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China.===</mods:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Correspondence address: Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y. M." last="Liu">Esther Y. M. Liu</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Leung, Danny T M" sort="Leung, Danny T M" uniqKey="Leung D" first="Danny T. M." last="Leung">Danny T. M. Leung</name><affiliation wicri:level="1"><mods:affiliation>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L. K." last="Cheung">Jo L. K. Cheung</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Ma, C H" sort="Ma, C H" uniqKey="Ma C" first="C. H." last="Ma">C. H. Ma</name><affiliation wicri:level="1"><mods:affiliation>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Tam, Frankie C H" sort="Tam, Frankie C H" uniqKey="Tam F" first="Frankie C. H." last="Tam">Frankie C. H. Tam</name><affiliation wicri:level="1"><mods:affiliation>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name><affiliation wicri:level="1"><mods:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S." last="Tam">John S. Tam</name><affiliation wicri:level="1"><mods:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author><author><name sortKey="Lim, Pak Leong" sort="Lim, Pak Leong" uniqKey="Lim P" first="Pak Leong" last="Lim">Pak Leong Lim</name><affiliation wicri:level="1"><mods:affiliation>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">75</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="181">181</biblScope><biblScope unit="page" to="184">184</biblScope><biblScope unit="page-count">4</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2005-02">2005-02</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antibody response</term><term>Assay</term><term>Chan</term><term>Chinese university</term><term>Clin</term><term>Clin diagn</term><term>Coronavirus</term><term>Coronavirus nucleocapsid protein</term><term>Early samples</term><term>Elisarstm</term><term>Enzyme immunoassay</term><term>Hong kong</term><term>Iggene</term><term>Immunoassay</term><term>Novel coronavirus</term><term>Nucleocapsid</term><term>Nucleocapsid protein</term><term>Peiris</term><term>Recombinant</term><term>Respiratory syndrome</term><term>Respiratory syndrome coronavirus</term><term>Sars</term><term>Sars patients</term><term>Serum samples</term><term>Syndrome</term><term>Wales hospital</term><term>Wang</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A high throughput accurate assay for anti‐SARS‐CoV IgG detection is needed for large‐scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein‐based microtitre plate enzyme immunoassay, ELISARS™ is described. The results on 150 sera from SARS patients and 450 sera from non‐SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large‐scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti‐nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation. J. Med. Virol. 75:181–184, 2005. © 2004 Wiley‐Liss, Inc.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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