Tracking Pathogen Transmission at the Human–Wildlife Interface: Banded Mongoose and Escherichia coli
Identifieur interne : 001E60 ( Istex/Corpus ); précédent : 001E59; suivant : 001E61Tracking Pathogen Transmission at the Human–Wildlife Interface: Banded Mongoose and Escherichia coli
Auteurs : R. Pesapane ; M. Ponder ; K. A. AlexanderSource :
- EcoHealth [ 1612-9202 ] ; 2013-06-01.
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- KwdEn :
Abstract
Abstract: A primary challenge to managing emerging infectious disease is identifying pathways that allow pathogen transmission at the human–wildlife interface. Using Escherichia coli as a model organism, we evaluated fecal bacterial transmission between banded mongoose (Mungos mungo) and humans in northern Botswana. Fecal samples were collected from banded mongoose living in protected areas (n = 87, 3 troops) and surrounding villages (n = 92, 3 troops). Human fecal waste was collected from the same environment (n = 46). Isolates were evaluated for susceptibility to 10 antibiotics. Resistant E. coli isolates from mongoose were compared to human isolates using rep-PCR fingerprinting and MLST-PCR. Antimicrobial resistant isolates were identified in 57 % of the mongoose fecal samples tested (range 31–78% among troops). At least one individual mongoose fecal sample demonstrated resistance to each tested antibiotic, and multidrug resistance was highest in the protected areas (40.9%). E. coli isolated from mongoose and human sources in this study demonstrated an extremely high degree of genetic similarity on rep-PCR (AMOVA, F ST = 0.0027, p = 0.18) with a similar pattern identified on MLST-PCR. Human waste may be an important source of microbial exposure to wildlife. Evidence of high levels of antimicrobial resistance even within protected areas identifies an emerging health threat and highlights the need for improved waste management in these systems.
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DOI: 10.1007/s10393-013-0838-2
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ISTEX:7354329C1D049BA151AB81E0754242FD390266F8Le document en format XML
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Pesapane</name><affiliation><mods:affiliation>Department of Fish and Wildlife Conservation, Virginia Tech, Virginia Polytechnic Institute and State University, Fish and Wildlife Conservation, 152 Cheatham Hall (0321), 24061, Blacksburg, VA, USA</mods:affiliation></affiliation></author><author><name sortKey="Ponder, M" sort="Ponder, M" uniqKey="Ponder M" first="M." last="Ponder">M. Ponder</name><affiliation><mods:affiliation>Department of Food Science and Technology, Virginia Tech, Blacksburg, VA, USA</mods:affiliation></affiliation></author><author><name sortKey="Alexander, K A" sort="Alexander, K A" uniqKey="Alexander K" first="K. A." last="Alexander">K. A. Alexander</name><affiliation><mods:affiliation>Department of Fish and Wildlife Conservation, Virginia Tech, Virginia Polytechnic Institute and State University, Fish and Wildlife Conservation, 152 Cheatham Hall (0321), 24061, Blacksburg, VA, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>CARACAL: Centre for Conservation of African Resources Animals, Communities and Land Use, Kasane, Botswana</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: kathyalx@vt.edu</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">EcoHealth</title><title level="j" type="sub">Conservation Medicine: Human Health:Ecosystem Sustainability Official journal of International Association for Ecology and Health</title><title level="j" type="abbrev">EcoHealth</title><idno type="ISSN">1612-9202</idno><idno type="eISSN">1612-9210</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>New York</pubPlace><date type="published" when="2013-06-01">2013-06-01</date><biblScope unit="volume">10</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="115">115</biblScope><biblScope unit="page" to="128">128</biblScope></imprint><idno type="ISSN">1612-9202</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1612-9202</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Escherichia coli</term><term>antibiotic resistance</term><term>banded mongoose</term><term>emerging infectious disease</term><term>human waste</term><term>zoonotic</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A primary challenge to managing emerging infectious disease is identifying pathways that allow pathogen transmission at the human–wildlife interface. Using Escherichia coli as a model organism, we evaluated fecal bacterial transmission between banded mongoose (Mungos mungo) and humans in northern Botswana. Fecal samples were collected from banded mongoose living in protected areas (n = 87, 3 troops) and surrounding villages (n = 92, 3 troops). Human fecal waste was collected from the same environment (n = 46). Isolates were evaluated for susceptibility to 10 antibiotics. Resistant E. coli isolates from mongoose were compared to human isolates using rep-PCR fingerprinting and MLST-PCR. Antimicrobial resistant isolates were identified in 57 % of the mongoose fecal samples tested (range 31–78% among troops). At least one individual mongoose fecal sample demonstrated resistance to each tested antibiotic, and multidrug resistance was highest in the protected areas (40.9%). E. coli isolated from mongoose and human sources in this study demonstrated an extremely high degree of genetic similarity on rep-PCR (AMOVA, F ST = 0.0027, p = 0.18) with a similar pattern identified on MLST-PCR. Human waste may be an important source of microbial exposure to wildlife. Evidence of high levels of antimicrobial resistance even within protected areas identifies an emerging health threat and highlights the need for improved waste management in these systems.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>R. Pesapane</name><affiliations><json:string>Department of Fish and Wildlife Conservation, Virginia Tech, Virginia Polytechnic Institute and State University, Fish and Wildlife Conservation, 152 Cheatham Hall (0321), 24061, Blacksburg, VA, USA</json:string></affiliations></json:item><json:item><name>M. 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Using Escherichia coli as a model organism, we evaluated fecal bacterial transmission between banded mongoose (Mungos mungo) and humans in northern Botswana. Fecal samples were collected from banded mongoose living in protected areas (n = 87, 3 troops) and surrounding villages (n = 92, 3 troops). Human fecal waste was collected from the same environment (n = 46). Isolates were evaluated for susceptibility to 10 antibiotics. Resistant E. coli isolates from mongoose were compared to human isolates using rep-PCR fingerprinting and MLST-PCR. Antimicrobial resistant isolates were identified in 57 % of the mongoose fecal samples tested (range 31–78% among troops). At least one individual mongoose fecal sample demonstrated resistance to each tested antibiotic, and multidrug resistance was highest in the protected areas (40.9%). E. coli isolated from mongoose and human sources in this study demonstrated an extremely high degree of genetic similarity on rep-PCR (AMOVA, F ST = 0.0027, p = 0.18) with a similar pattern identified on MLST-PCR. Human waste may be an important source of microbial exposure to wildlife. 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Using Escherichia coli as a model organism, we evaluated fecal bacterial transmission between banded mongoose (Mungos mungo) and humans in northern Botswana. Fecal samples were collected from banded mongoose living in protected areas (n = 87, 3 troops) and surrounding villages (n = 92, 3 troops). Human fecal waste was collected from the same environment (n = 46). Isolates were evaluated for susceptibility to 10 antibiotics. Resistant E. coli isolates from mongoose were compared to human isolates using rep-PCR fingerprinting and MLST-PCR. Antimicrobial resistant isolates were identified in 57 % of the mongoose fecal samples tested (range 31–78% among troops). At least one individual mongoose fecal sample demonstrated resistance to each tested antibiotic, and multidrug resistance was highest in the protected areas (40.9%). E. coli isolated from mongoose and human sources in this study demonstrated an extremely high degree of genetic similarity on rep-PCR (AMOVA, F ST = 0.0027, p = 0.18) with a similar pattern identified on MLST-PCR. Human waste may be an important source of microbial exposure to wildlife. Evidence of high levels of antimicrobial resistance even within protected areas identifies an emerging health threat and highlights the need for improved waste management in these systems.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>zoonotic</term></item><item><term>Escherichia coli</term></item><item><term>banded mongoose</term></item><item><term>human waste</term></item><item><term>emerging infectious disease</term></item><item><term>antibiotic resistance</term></item></list></keywords></textClass><textClass><keywords scheme="Journal-Subject-Collection"><list><label>SC11</label><item><term>Medicine</term></item></list></keywords></textClass><textClass><keywords scheme="Journal-Subject-Group"><list><label>SCH</label><item><term>Medicine & Public Health</term></item><label>SCH27002</label><item><term>Public Health</term></item><label>SCU18005</label><item><term>Environmental 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coli</Emphasis></ArticleTitle><ArticleCategory>Original Contribution</ArticleCategory><ArticleFirstPage>115</ArticleFirstPage><ArticleLastPage>128</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2013</Year><Month>4</Month><Day>2</Day></RegistrationDate><Received><Year>2012</Year><Month>10</Month><Day>29</Day></Received><Revised><Year>2013</Year><Month>3</Month><Day>11</Day></Revised><Accepted><Year>2013</Year><Month>3</Month><Day>24</Day></Accepted><OnlineDate><Year>2013</Year><Month>4</Month><Day>24</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>International Association for Ecology and Health</CopyrightHolderName><CopyrightYear>2013</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>R.</GivenName><FamilyName>Pesapane</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><FamilyName>Ponder</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff3" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>K.</GivenName><GivenName>A.</GivenName><FamilyName>Alexander</FamilyName></AuthorName><Contact><Phone>540-231-5059</Phone><Email>kathyalx@vt.edu</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Department of Fish and Wildlife Conservation</OrgDivision><OrgName>Virginia Tech, Virginia Polytechnic Institute and State University</OrgName><OrgAddress><Street>Fish and Wildlife Conservation, 152 Cheatham Hall (0321)</Street><City>Blacksburg</City><State>VA</State><Postcode>24061</Postcode><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Food Science and Technology</OrgDivision><OrgName>Virginia Tech</OrgName><OrgAddress><City>Blacksburg</City><State>VA</State><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>CARACAL: Centre for Conservation of African Resources Animals</OrgDivision><OrgName>Communities and Land Use</OrgName><OrgAddress><City>Kasane</City><Country Code="BW">Botswana</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>A primary challenge to managing emerging infectious disease is identifying pathways that allow pathogen transmission at the human–wildlife interface. Using <Emphasis Type="Italic">Escherichia coli</Emphasis> as a model organism, we evaluated fecal bacterial transmission between banded mongoose (<Emphasis Type="Italic">Mungos mungo</Emphasis>) and humans in northern Botswana. Fecal samples were collected from banded mongoose living in protected areas (<Emphasis Type="Italic">n</Emphasis> = 87, 3 troops) and surrounding villages (<Emphasis Type="Italic">n</Emphasis> = 92, 3 troops). Human fecal waste was collected from the same environment (<Emphasis Type="Italic">n</Emphasis> = 46). Isolates were evaluated for susceptibility to 10 antibiotics. Resistant <Emphasis Type="Italic">E. coli</Emphasis> isolates from mongoose were compared to human isolates using rep-PCR fingerprinting and MLST-PCR. Antimicrobial resistant isolates were identified in 57 % of the mongoose fecal samples tested (range 31–78% among troops). At least one individual mongoose fecal sample demonstrated resistance to each tested antibiotic, and multidrug resistance was highest in the protected areas (40.9%). <Emphasis Type="Italic">E. coli</Emphasis> isolated from mongoose and human sources in this study demonstrated an extremely high degree of genetic similarity on rep-PCR (AMOVA, <Emphasis Type="Italic">F</Emphasis><Subscript>ST</Subscript> = 0.0027, <Emphasis Type="Italic">p</Emphasis> = 0.18) with a similar pattern identified on MLST-PCR. Human waste may be an important source of microbial exposure to wildlife. Evidence of high levels of antimicrobial resistance even within protected areas identifies an emerging health threat and highlights the need for improved waste management in these systems.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>zoonotic</Keyword><Keyword><Emphasis Type="Italic">Escherichia coli</Emphasis></Keyword><Keyword>banded mongoose</Keyword><Keyword>human waste</Keyword><Keyword>emerging infectious disease</Keyword><Keyword>antibiotic resistance</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Tracking Pathogen Transmission at the Human–Wildlife Interface: Banded Mongoose and Escherichia coli</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Tracking Pathogen Transmission at the Human–Wildlife Interface: Banded Mongoose and Escherichia coli </title></titleInfo><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Pesapane</namePart><affiliation>Department of Fish and Wildlife Conservation, Virginia Tech, Virginia Polytechnic Institute and State University, Fish and Wildlife Conservation, 152 Cheatham Hall (0321), 24061, Blacksburg, VA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Ponder</namePart><affiliation>Department of Food Science and Technology, Virginia Tech, Blacksburg, VA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">K.</namePart><namePart type="given">A.</namePart><namePart type="family">Alexander</namePart><affiliation>Department of Fish and Wildlife Conservation, Virginia Tech, Virginia Polytechnic Institute and State University, Fish and Wildlife Conservation, 152 Cheatham Hall (0321), 24061, Blacksburg, VA, USA</affiliation><affiliation>CARACAL: Centre for Conservation of African Resources Animals, Communities and Land Use, Kasane, Botswana</affiliation><affiliation>E-mail: kathyalx@vt.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">New York</placeTerm></place><dateCreated encoding="w3cdtf">2012-10-29</dateCreated><dateIssued encoding="w3cdtf">2013-06-01</dateIssued><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: A primary challenge to managing emerging infectious disease is identifying pathways that allow pathogen transmission at the human–wildlife interface. Using Escherichia coli as a model organism, we evaluated fecal bacterial transmission between banded mongoose (Mungos mungo) and humans in northern Botswana. Fecal samples were collected from banded mongoose living in protected areas (n = 87, 3 troops) and surrounding villages (n = 92, 3 troops). Human fecal waste was collected from the same environment (n = 46). Isolates were evaluated for susceptibility to 10 antibiotics. Resistant E. coli isolates from mongoose were compared to human isolates using rep-PCR fingerprinting and MLST-PCR. Antimicrobial resistant isolates were identified in 57 % of the mongoose fecal samples tested (range 31–78% among troops). At least one individual mongoose fecal sample demonstrated resistance to each tested antibiotic, and multidrug resistance was highest in the protected areas (40.9%). E. coli isolated from mongoose and human sources in this study demonstrated an extremely high degree of genetic similarity on rep-PCR (AMOVA, F ST = 0.0027, p = 0.18) with a similar pattern identified on MLST-PCR. Human waste may be an important source of microbial exposure to wildlife. Evidence of high levels of antimicrobial resistance even within protected areas identifies an emerging health threat and highlights the need for improved waste management in these systems.</abstract><note>Original Contribution</note><subject lang="en"><genre>Keywords</genre><topic>zoonotic</topic><topic>Escherichia coli</topic><topic>banded mongoose</topic><topic>human waste</topic><topic>emerging infectious disease</topic><topic>antibiotic resistance</topic></subject><relatedItem type="host"><titleInfo><title>EcoHealth</title><subTitle>Conservation Medicine: Human Health:Ecosystem Sustainability Official journal of International Association for Ecology and Health</subTitle></titleInfo><titleInfo type="abbreviated"><title>EcoHealth</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2013-06-19</dateIssued><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><subject><genre>Journal-Subject-Collection</genre><topic authority="SpringerSubjectCodes" authorityURI="SC11">Medicine</topic></subject><subject><genre>Journal-Subject-Group</genre><topic authority="SpringerSubjectCodes" authorityURI="SCH">Medicine & Public Health</topic><topic authority="SpringerSubjectCodes" authorityURI="SCH27002">Public Health</topic><topic authority="SpringerSubjectCodes" authorityURI="SCU18005">Environmental Health</topic><topic authority="SpringerSubjectCodes" authorityURI="SCL1904X">Ecosystems</topic><topic authority="SpringerSubjectCodes" authorityURI="SCU17009">Environmental Management</topic><topic authority="SpringerSubjectCodes" authorityURI="SCL23004">Microbiology</topic><topic authority="SpringerSubjectCodes" authorityURI="SCL19015">Animal Ecology</topic></subject><identifier type="ISSN">1612-9202</identifier><identifier type="eISSN">1612-9210</identifier><identifier type="JournalID">10393</identifier><identifier type="IssueArticleCount">16</identifier><identifier type="VolumeIssueCount">4</identifier><part><date>2013</date><detail type="volume"><number>10</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="pages"><start>115</start><end>128</end></extent></part><recordInfo><recordOrigin>International Association for Ecology and Health, 2013</recordOrigin></recordInfo></relatedItem><identifier type="istex">7354329C1D049BA151AB81E0754242FD390266F8</identifier><identifier type="ark">ark:/67375/VQC-BKXWB0CC-F</identifier><identifier type="DOI">10.1007/s10393-013-0838-2</identifier><identifier type="ArticleID">838</identifier><identifier type="ArticleID">s10393-013-0838-2</identifier><accessCondition type="use and reproduction" contentType="copyright">International Association for Ecology and Health, 2013</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>International Association for Ecology and Health, 2013</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-BKXWB0CC-F/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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