Sequential biogenesis of host cell membrane rearrangements induced by hepatitis C virus infection
Identifieur interne : 001439 ( Istex/Corpus ); précédent : 001438; suivant : 001440Sequential biogenesis of host cell membrane rearrangements induced by hepatitis C virus infection
Auteurs : Pauline Ferraris ; Elodie Beaumont ; Rustem Uzbekov ; Denys Brand ; Julien Gaillard ; Emmanuelle Blanchard ; Philippe RoingeardSource :
- Cellular and Molecular Life Sciences [ 1420-682X ] ; 2013-04-01.
English descriptors
Abstract
Abstract: Like most positive-strand RNA viruses, hepatitis C virus (HCV) forms a membrane-associated replication complex consisting of replicating RNA, viral and host proteins anchored to altered cell membranes. We used a combination of qualitative and quantitative electron microscopy (EM), immuno-EM, and the 3D reconstruction of serial EM sections to analyze the host cell membrane alterations induced by HCV. Three different types of membrane alteration were observed: vesicles in clusters (ViCs), contiguous vesicles (CVs), and double-membrane vesicles (DMVs). The main ultrastructural change observed early in infection was the formation of a network of CVs surrounding the lipid droplets. Later stages in the infectious cycle were characterized by a large increase in the number of DMVs, which may be derived from the CVs. These DMVs are thought to constitute the membranous structures harboring the viral replication complexes in which viral replication is firmly and permanently established and to protect the virus against double-stranded RNA-triggered host antiviral responses.
Url:
DOI: 10.1007/s00018-012-1213-0
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ISTEX:FB88C65A15C64A2810A3E9A2DC597CEB63602FC0Le document en format XML
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Life Sci.</title><idno type="ISSN">1420-682X</idno><idno type="eISSN">1420-9071</idno><imprint><publisher>SP Birkhäuser Verlag Basel</publisher><pubPlace>Basel</pubPlace><date type="published" when="2013-04-01">2013-04-01</date><biblScope unit="volume">70</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1297">1297</biblScope><biblScope unit="page" to="1306">1306</biblScope></imprint><idno type="ISSN">1420-682X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1420-682X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3D reconstruction</term><term>Electron microscopy</term><term>HCV</term><term>Host cell membranes</term><term>Membranous web</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Like most positive-strand RNA viruses, hepatitis C virus (HCV) forms a membrane-associated replication complex consisting of replicating RNA, viral and host proteins anchored to altered cell membranes. We used a combination of qualitative and quantitative electron microscopy (EM), immuno-EM, and the 3D reconstruction of serial EM sections to analyze the host cell membrane alterations induced by HCV. Three different types of membrane alteration were observed: vesicles in clusters (ViCs), contiguous vesicles (CVs), and double-membrane vesicles (DMVs). The main ultrastructural change observed early in infection was the formation of a network of CVs surrounding the lipid droplets. Later stages in the infectious cycle were characterized by a large increase in the number of DMVs, which may be derived from the CVs. These DMVs are thought to constitute the membranous structures harboring the viral replication complexes in which viral replication is firmly and permanently established and to protect the virus against double-stranded RNA-triggered host antiviral responses.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Pauline Ferraris</name><affiliations><json:string>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</json:string></affiliations></json:item><json:item><name>Elodie Beaumont</name><affiliations><json:string>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</json:string></affiliations></json:item><json:item><name>Rustem Uzbekov</name><affiliations><json:string>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</json:string></affiliations></json:item><json:item><name>Denys Brand</name><affiliations><json:string>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</json:string></affiliations></json:item><json:item><name>Julien Gaillard</name><affiliations><json:string>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</json:string></affiliations></json:item><json:item><name>Emmanuelle Blanchard</name><affiliations><json:string>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</json:string><json:string>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</json:string></affiliations></json:item><json:item><name>Philippe Roingeard</name><affiliations><json:string>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</json:string><json:string>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</json:string><json:string>E-mail: roingeard@med.univ-tours.fr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>HCV</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Host cell membranes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Membranous web</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Electron microscopy</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>3D reconstruction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>BSA : Bovine serum albumin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CM : Convoluted membranes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CV : Contiguous vesicles</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DENV : Dengue virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DMEM : Dulbecco’s modified Eagle medium</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DMV : Double-membrane vesicle</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>EM : Electron microscopy</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>FFU : Focus forming unit</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HCV : Hepatitis C virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>KUNV : Kunjin virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LD : Lipid droplet</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>MAbs : Mouse monoclonal antibody</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>MMV : Multimembrane vesicles</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>MVB : Multivesicular bodies</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ViC : Vesicles in cluster</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>VP : Vesicle packets</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>WNV : West Nile virus</value></json:item></subject><articleId><json:string>1213</json:string><json:string>s00018-012-1213-0</json:string></articleId><arkIstex>ark:/67375/VQC-5X7HMXKM-B</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Like most positive-strand RNA viruses, hepatitis C virus (HCV) forms a membrane-associated replication complex consisting of replicating RNA, viral and host proteins anchored to altered cell membranes. We used a combination of qualitative and quantitative electron microscopy (EM), immuno-EM, and the 3D reconstruction of serial EM sections to analyze the host cell membrane alterations induced by HCV. Three different types of membrane alteration were observed: vesicles in clusters (ViCs), contiguous vesicles (CVs), and double-membrane vesicles (DMVs). The main ultrastructural change observed early in infection was the formation of a network of CVs surrounding the lipid droplets. Later stages in the infectious cycle were characterized by a large increase in the number of DMVs, which may be derived from the CVs. These DMVs are thought to constitute the membranous structures harboring the viral replication complexes in which viral replication is firmly and permanently established and to protect the virus against double-stranded RNA-triggered host antiviral responses.</abstract><qualityIndicators><score>8.598</score><pdfWordCount>4738</pdfWordCount><pdfCharCount>29631</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>595.276 x 790.866 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>155</abstractWordCount><abstractCharCount>1088</abstractCharCount><keywordCount>22</keywordCount></qualityIndicators><title>Sequential biogenesis of host cell membrane rearrangements induced by hepatitis C virus infection</title><genre><json:string>research-article</json:string></genre><host><title>Cellular and Molecular Life 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type="first">Pauline</forename><surname>Ferraris</surname></persName><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Elodie</forename><surname>Beaumont</surname></persName><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Rustem</forename><surname>Uzbekov</surname></persName><affiliation>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Denys</forename><surname>Brand</surname></persName><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Julien</forename><surname>Gaillard</surname></persName><affiliation>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Emmanuelle</forename><surname>Blanchard</surname></persName><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation><affiliation>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</affiliation></author><author xml:id="author-0006" corresp="yes"><persName><forename type="first">Philippe</forename><surname>Roingeard</surname></persName><email>roingeard@med.univ-tours.fr</email><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation><affiliation>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</affiliation></author><idno type="istex">FB88C65A15C64A2810A3E9A2DC597CEB63602FC0</idno><idno type="ark">ark:/67375/VQC-5X7HMXKM-B</idno><idno type="DOI">10.1007/s00018-012-1213-0</idno><idno type="article-id">1213</idno><idno type="article-id">s00018-012-1213-0</idno></analytic><monogr><title level="j">Cellular and Molecular Life Sciences</title><title level="j" type="abbrev">Cell. Mol. Life Sci.</title><idno type="pISSN">1420-682X</idno><idno type="eISSN">1420-9071</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">11</idno><idno type="volume-issue-count">24</idno><imprint><publisher>SP Birkhäuser Verlag Basel</publisher><pubPlace>Basel</pubPlace><date type="published" when="2013-04-01"></date><biblScope unit="volume">70</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1297">1297</biblScope><biblScope unit="page" to="1306">1306</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-06-30</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Like most positive-strand RNA viruses, hepatitis C virus (HCV) forms a membrane-associated replication complex consisting of replicating RNA, viral and host proteins anchored to altered cell membranes. We used a combination of qualitative and quantitative electron microscopy (EM), immuno-EM, and the 3D reconstruction of serial EM sections to analyze the host cell membrane alterations induced by HCV. Three different types of membrane alteration were observed: vesicles in clusters (ViCs), contiguous vesicles (CVs), and double-membrane vesicles (DMVs). The main ultrastructural change observed early in infection was the formation of a network of CVs surrounding the lipid droplets. Later stages in the infectious cycle were characterized by a large increase in the number of DMVs, which may be derived from the CVs. These DMVs are thought to constitute the membranous structures harboring the viral replication complexes in which viral replication is firmly and permanently established and to protect the virus against double-stranded RNA-triggered host antiviral responses.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>HCV</term></item><item><term>Host cell membranes</term></item><item><term>Membranous web</term></item><item><term>Electron microscopy</term></item><item><term>3D reconstruction</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>BSA</term><term>Bovine serum albumin</term></item><item><term>CM</term><term>Convoluted membranes</term></item><item><term>CV</term><term>Contiguous vesicles</term></item><item><term>DENV</term><term>Dengue virus</term></item><item><term>DMEM</term><term>Dulbecco’s modified Eagle medium</term></item><item><term>DMV</term><term>Double-membrane vesicle</term></item><item><term>EM</term><term>Electron microscopy</term></item><item><term>FFU</term><term>Focus forming unit</term></item><item><term>HCV</term><term>Hepatitis C virus</term></item><item><term>KUNV</term><term>Kunjin virus</term></item><item><term>LD</term><term>Lipid droplet</term></item><item><term>MAbs</term><term>Mouse monoclonal antibody</term></item><item><term>MMV</term><term>Multimembrane vesicles</term></item><item><term>MVB</term><term>Multivesicular bodies</term></item><item><term>ViC</term><term>Vesicles in cluster</term></item><item><term>VP</term><term>Vesicle packets</term></item><item><term>WNV</term><term>West Nile virus</term></item></list></keywords></textClass><textClass><keywords scheme="Journal-Subject-Collection"><list><label>SC3</label><item><term>Biomedical and Life Sciences</term></item></list></keywords></textClass><textClass><keywords scheme="Journal-Subject-Group"><list><label>SCL</label><item><term>Life Sciences</term></item><label>SCL16008</label><item><term>Cell Biology</term></item><label>SCB0000X</label><item><term>Biomedicine general</term></item><label>SCL00004</label><item><term>Life Sciences, general</term></item><label>SCL14005</label><item><term>Biochemistry, general</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-06-30">Created</change><change when="2013-04-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-5X7HMXKM-B/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>SP Birkhäuser Verlag Basel</PublisherName><PublisherLocation>Basel</PublisherLocation><PublisherImprintName>Birkhäuser</PublisherImprintName></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="NonStandardArchiveJournal" NumberingStyle="Unnumbered"><JournalID>18</JournalID><JournalPrintISSN>1420-682X</JournalPrintISSN><JournalElectronicISSN>1420-9071</JournalElectronicISSN><JournalTitle>Cellular and Molecular Life Sciences</JournalTitle><JournalAbbreviatedTitle>Cell. Mol. Life Sci.</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Code="SCL" Type="Primary">Life Sciences</JournalSubject><JournalSubject Code="SCL16008" Priority="1" Type="Secondary">Cell Biology</JournalSubject><JournalSubject Code="SCB0000X" Priority="2" Type="Secondary">Biomedicine general</JournalSubject><JournalSubject Code="SCL00004" Priority="3" Type="Secondary">Life Sciences, general</JournalSubject><JournalSubject Code="SCL14005" Priority="4" Type="Secondary">Biochemistry, general</JournalSubject><SubjectCollection Code="SC3">Biomedical and Life Sciences</SubjectCollection></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>70</VolumeIDStart><VolumeIDEnd>70</VolumeIDEnd><VolumeIssueCount>24</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>7</IssueIDStart><IssueIDEnd>7</IssueIDEnd><IssueArticleCount>11</IssueArticleCount><IssueHistory><OnlineDate><Year>2013</Year><Month>3</Month><Day>13</Day></OnlineDate><PrintDate><Year>2013</Year><Month>3</Month><Day>12</Day></PrintDate><CoverDate><Year>2013</Year><Month>4</Month></CoverDate><PricelistYear>2013</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Basel</CopyrightHolderName><CopyrightYear>2013</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s00018-012-1213-0" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="Yes" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>1213</ArticleID><ArticleDOI>10.1007/s00018-012-1213-0</ArticleDOI><ArticleSequenceNumber>11</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Sequential biogenesis of host cell membrane rearrangements induced by hepatitis C virus infection</ArticleTitle><ArticleCategory>Research Article</ArticleCategory><ArticleFirstPage>1297</ArticleFirstPage><ArticleLastPage>1306</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2012</Year><Month>11</Month><Day>7</Day></RegistrationDate><Received><Year>2012</Year><Month>6</Month><Day>30</Day></Received><Revised><Year>2012</Year><Month>10</Month><Day>13</Day></Revised><Accepted><Year>2012</Year><Month>11</Month><Day>7</Day></Accepted><OnlineDate><Year>2012</Year><Month>11</Month><Day>25</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Basel</CopyrightHolderName><CopyrightYear>2012</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Pauline</GivenName><FamilyName>Ferraris</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Elodie</GivenName><FamilyName>Beaumont</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Rustem</GivenName><FamilyName>Uzbekov</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Denys</GivenName><FamilyName>Brand</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Julien</GivenName><FamilyName>Gaillard</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Emmanuelle</GivenName><FamilyName>Blanchard</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Philippe</GivenName><FamilyName>Roingeard</FamilyName></AuthorName><Contact><Phone>+33-2-34379646</Phone><Fax>+33-2-47478207</Fax><Email>roingeard@med.univ-tours.fr</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>INSERM U966, Faculté de Médecine</OrgDivision><OrgName>Université François Rabelais de Tours, CHRU de Tours</OrgName><OrgAddress><Street>10 boulevard Tonnellé</Street><Postcode>37032</Postcode><City>Tours Cedex</City><Country Code="FR">France</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Plate-Forme des Microscopies, PPF ASB</OrgDivision><OrgName>Université François Rabelais & CHRU de Tours</OrgName><OrgAddress><City>Tours Cedex</City><Country Code="FR">France</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>Like most positive-strand RNA viruses, hepatitis C virus (HCV) forms a membrane-associated replication complex consisting of replicating RNA, viral and host proteins anchored to altered cell membranes. We used a combination of qualitative and quantitative electron microscopy (EM), immuno-EM, and the 3D reconstruction of serial EM sections to analyze the host cell membrane alterations induced by HCV. Three different types of membrane alteration were observed: vesicles in clusters (ViCs), contiguous vesicles (CVs), and double-membrane vesicles (DMVs). The main ultrastructural change observed early in infection was the formation of a network of CVs surrounding the lipid droplets. Later stages in the infectious cycle were characterized by a large increase in the number of DMVs, which may be derived from the CVs. These DMVs are thought to constitute the membranous structures harboring the viral replication complexes in which viral replication is firmly and permanently established and to protect the virus against double-stranded RNA-triggered host antiviral responses.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>HCV</Keyword><Keyword>Host cell membranes</Keyword><Keyword>Membranous web</Keyword><Keyword>Electron microscopy</Keyword><Keyword>3D reconstruction</Keyword></KeywordGroup><AbbreviationGroup><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term>BSA</Term><Description><Para>Bovine serum albumin</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>CM</Term><Description><Para>Convoluted membranes</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>CV</Term><Description><Para>Contiguous vesicles</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>DENV</Term><Description><Para>Dengue virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>DMEM</Term><Description><Para>Dulbecco’s modified Eagle medium</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>DMV</Term><Description><Para>Double-membrane vesicle</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>EM</Term><Description><Para>Electron microscopy</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>FFU</Term><Description><Para>Focus forming unit</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>HCV</Term><Description><Para>Hepatitis C virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>KUNV</Term><Description><Para>Kunjin virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>LD</Term><Description><Para>Lipid droplet</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>MAbs</Term><Description><Para>Mouse monoclonal antibody</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>MMV</Term><Description><Para>Multimembrane vesicles</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>MVB</Term><Description><Para>Multivesicular bodies</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>ViC</Term><Description><Para>Vesicles in cluster</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>VP</Term><Description><Para>Vesicle packets</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>WNV</Term><Description><Para>West Nile virus</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroup><ArticleNote Type="Misc"><SimplePara>E. Blanchard and P. Roingeard are co-last authors.</SimplePara></ArticleNote><ArticleNote Type="ESMHint"><Heading>Electronic supplementary material</Heading><SimplePara>The online version of this article (doi:<ExternalRef><RefSource>10.1007/s00018-012-1213-0</RefSource><RefTarget Address="10.1007/s00018-012-1213-0" TargetType="DOI"></RefTarget></ExternalRef>) contains supplementary material, which is available to authorized users.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Sequential biogenesis of host cell membrane rearrangements induced by hepatitis C virus infection</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Sequential biogenesis of host cell membrane rearrangements induced by hepatitis C virus infection</title></titleInfo><name type="personal"><namePart type="given">Pauline</namePart><namePart type="family">Ferraris</namePart><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Elodie</namePart><namePart type="family">Beaumont</namePart><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rustem</namePart><namePart type="family">Uzbekov</namePart><affiliation>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Denys</namePart><namePart type="family">Brand</namePart><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Julien</namePart><namePart type="family">Gaillard</namePart><affiliation>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Emmanuelle</namePart><namePart type="family">Blanchard</namePart><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation><affiliation>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Philippe</namePart><namePart type="family">Roingeard</namePart><affiliation>INSERM U966, Faculté de Médecine, Université François Rabelais de Tours, CHRU de Tours, 10 boulevard Tonnellé, 37032, Tours Cedex, France</affiliation><affiliation>Plate-Forme des Microscopies, PPF ASB, Université François Rabelais & CHRU de Tours, Tours Cedex, France</affiliation><affiliation>E-mail: roingeard@med.univ-tours.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>SP Birkhäuser Verlag Basel</publisher><place><placeTerm type="text">Basel</placeTerm></place><dateCreated encoding="w3cdtf">2012-06-30</dateCreated><dateIssued encoding="w3cdtf">2013-04-01</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Like most positive-strand RNA viruses, hepatitis C virus (HCV) forms a membrane-associated replication complex consisting of replicating RNA, viral and host proteins anchored to altered cell membranes. We used a combination of qualitative and quantitative electron microscopy (EM), immuno-EM, and the 3D reconstruction of serial EM sections to analyze the host cell membrane alterations induced by HCV. Three different types of membrane alteration were observed: vesicles in clusters (ViCs), contiguous vesicles (CVs), and double-membrane vesicles (DMVs). The main ultrastructural change observed early in infection was the formation of a network of CVs surrounding the lipid droplets. Later stages in the infectious cycle were characterized by a large increase in the number of DMVs, which may be derived from the CVs. These DMVs are thought to constitute the membranous structures harboring the viral replication complexes in which viral replication is firmly and permanently established and to protect the virus against double-stranded RNA-triggered host antiviral responses.</abstract><note>Research Article</note><subject lang="en"><genre>Keywords</genre><topic>HCV</topic><topic>Host cell membranes</topic><topic>Membranous web</topic><topic>Electron microscopy</topic><topic>3D reconstruction</topic></subject><subject><genre>Abbreviations</genre><topic>BSA : Bovine serum albumin</topic><topic>CM : Convoluted membranes</topic><topic>CV : Contiguous vesicles</topic><topic>DENV : Dengue virus</topic><topic>DMEM : Dulbecco’s modified Eagle medium</topic><topic>DMV : Double-membrane vesicle</topic><topic>EM : Electron microscopy</topic><topic>FFU : Focus forming unit</topic><topic>HCV : Hepatitis C virus</topic><topic>KUNV : Kunjin virus</topic><topic>LD : Lipid droplet</topic><topic>MAbs : Mouse monoclonal antibody</topic><topic>MMV : Multimembrane vesicles</topic><topic>MVB : Multivesicular bodies</topic><topic>ViC : Vesicles in cluster</topic><topic>VP : Vesicle packets</topic><topic>WNV : West Nile virus</topic></subject><relatedItem type="host"><titleInfo><title>Cellular and Molecular Life Sciences</title></titleInfo><titleInfo type="abbreviated"><title>Cell. 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