A DNA miniarray system for simultaneous visual detection of porcine circovirus type 1 (PCV1) and 2 (PCV2) in pigs
Identifieur interne : 001318 ( Istex/Corpus ); précédent : 001317; suivant : 001319A DNA miniarray system for simultaneous visual detection of porcine circovirus type 1 (PCV1) and 2 (PCV2) in pigs
Auteurs : D. J. An ; D. S. Song ; J. Y. Park ; B. K. ParkSource :
- Veterinary Research Communications [ 0165-7380 ] ; 2009-02-01.
English descriptors
Abstract
Abstract: A miniarray system was developed for the simultaneous detection of porcine circovirus type 1 (PCV1) and type 2 (PCV2) in pigs. The system consists of a polymerase chain reaction (PCR) step to amplify target viral DNA, followed by detection of the amplified DNA using a membrane-anchored probe array and an avidin-alkaline phosphatase (Av-AP) indicator system. The lower limit of detection of PCV using the miniarray was 101.9 tissue culture infectious dose 50 (TCID50)/ml and 102.08TCID50/ml for PCV1 and PCV2, respectively, and 100 viral copies/μl for both PCV1 and PCV2. We validated the miniarray system using 141 lymph node specimens from pigs with suspected postweaning multisystemic wasting syndrome or porcine dermatitis and nephropathy syndrome. Of the 141 samples evaluated, 55 were identified as positive for PCV by the miniarray. Relative to in situ hybridization, the sensitivity and specificity of the miniarray was 100% and 98.9%, respectively. In contrast to other microarray systems, the miniarray does not require a DNA chip reader, since the results can be determined by visual inspection of colorized spots on a nylon membrane. This system represents an effective alternative method for the differential detection of PCV1 and PCV2 in pigs, as well as the maintenance of PCV-free cell lines and pre-screening of commercial vaccines for possible contamination.
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DOI: 10.1007/s11259-008-9080-8
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Park</name><affiliation><mods:affiliation>National Veterinary Research and Quarantine Service, Kyunggi-do, 430–824, Anyang, Korea</mods:affiliation></affiliation></author><author><name sortKey="Park, B K" sort="Park, B K" uniqKey="Park B" first="B. K." last="Park">B. K. Park</name><affiliation><mods:affiliation>College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, 151–742, Seoul, Korea</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: parkx026@snu.ac.kr</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F3B33621E228B8E5610F13090FE4CC356F110B97</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1007/s11259-008-9080-8</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-6FRD0T5R-S/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001318</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001318</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">A DNA miniarray system for simultaneous visual detection of porcine circovirus type 1 (PCV1) and 2 (PCV2) in pigs</title><author><name sortKey="An, D J" sort="An, D J" uniqKey="An D" first="D. J." last="An">D. J. An</name><affiliation><mods:affiliation>National Veterinary Research and Quarantine Service, Kyunggi-do, 430–824, Anyang, Korea</mods:affiliation></affiliation></author><author><name sortKey="Song, D S" sort="Song, D S" uniqKey="Song D" first="D. S." last="Song">D. S. Song</name><affiliation><mods:affiliation>Research Unit, Green Cross Veterinary Products, 449–903, Yong-in, Korea</mods:affiliation></affiliation></author><author><name sortKey="Park, J Y" sort="Park, J Y" uniqKey="Park J" first="J. Y." last="Park">J. Y. Park</name><affiliation><mods:affiliation>National Veterinary Research and Quarantine Service, Kyunggi-do, 430–824, Anyang, Korea</mods:affiliation></affiliation></author><author><name sortKey="Park, B K" sort="Park, B K" uniqKey="Park B" first="B. K." last="Park">B. K. Park</name><affiliation><mods:affiliation>College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, 151–742, Seoul, Korea</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: parkx026@snu.ac.kr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Veterinary Research Communications</title><title level="j" type="sub">An International Journal Publishing Topical Reviews and Research Articles on all Aspects of the Veterinary Sciences</title><title level="j" type="abbrev">Vet Res Commun</title><idno type="ISSN">0165-7380</idno><idno type="eISSN">1573-7446</idno><imprint><publisher>Springer Netherlands</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2009-02-01">2009-02-01</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="139">139</biblScope><biblScope unit="page" to="147">147</biblScope></imprint><idno type="ISSN">0165-7380</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-7380</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Miniarray</term><term>PCV1</term><term>PCV2</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A miniarray system was developed for the simultaneous detection of porcine circovirus type 1 (PCV1) and type 2 (PCV2) in pigs. The system consists of a polymerase chain reaction (PCR) step to amplify target viral DNA, followed by detection of the amplified DNA using a membrane-anchored probe array and an avidin-alkaline phosphatase (Av-AP) indicator system. The lower limit of detection of PCV using the miniarray was 101.9 tissue culture infectious dose 50 (TCID50)/ml and 102.08TCID50/ml for PCV1 and PCV2, respectively, and 100 viral copies/μl for both PCV1 and PCV2. We validated the miniarray system using 141 lymph node specimens from pigs with suspected postweaning multisystemic wasting syndrome or porcine dermatitis and nephropathy syndrome. Of the 141 samples evaluated, 55 were identified as positive for PCV by the miniarray. Relative to in situ hybridization, the sensitivity and specificity of the miniarray was 100% and 98.9%, respectively. In contrast to other microarray systems, the miniarray does not require a DNA chip reader, since the results can be determined by visual inspection of colorized spots on a nylon membrane. This system represents an effective alternative method for the differential detection of PCV1 and PCV2 in pigs, as well as the maintenance of PCV-free cell lines and pre-screening of commercial vaccines for possible contamination.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>D. J. An</name><affiliations><json:string>National Veterinary Research and Quarantine Service, Kyunggi-do, 430–824, Anyang, Korea</json:string></affiliations></json:item><json:item><name>D. S. Song</name><affiliations><json:string>Research Unit, Green Cross Veterinary Products, 449–903, Yong-in, Korea</json:string></affiliations></json:item><json:item><name>J. Y. 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Park</name><affiliations><json:string>College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, 151–742, Seoul, Korea</json:string><json:string>E-mail: parkx026@snu.ac.kr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Miniarray</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCV2</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCV1</value></json:item></subject><articleId><json:string>9080</json:string><json:string>s11259-008-9080-8</json:string></articleId><arkIstex>ark:/67375/VQC-6FRD0T5R-S</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: A miniarray system was developed for the simultaneous detection of porcine circovirus type 1 (PCV1) and type 2 (PCV2) in pigs. The system consists of a polymerase chain reaction (PCR) step to amplify target viral DNA, followed by detection of the amplified DNA using a membrane-anchored probe array and an avidin-alkaline phosphatase (Av-AP) indicator system. The lower limit of detection of PCV using the miniarray was 101.9 tissue culture infectious dose 50 (TCID50)/ml and 102.08TCID50/ml for PCV1 and PCV2, respectively, and 100 viral copies/μl for both PCV1 and PCV2. We validated the miniarray system using 141 lymph node specimens from pigs with suspected postweaning multisystemic wasting syndrome or porcine dermatitis and nephropathy syndrome. Of the 141 samples evaluated, 55 were identified as positive for PCV by the miniarray. Relative to in situ hybridization, the sensitivity and specificity of the miniarray was 100% and 98.9%, respectively. In contrast to other microarray systems, the miniarray does not require a DNA chip reader, since the results can be determined by visual inspection of colorized spots on a nylon membrane. 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The system consists of a polymerase chain reaction (PCR) step to amplify target viral DNA, followed by detection of the amplified DNA using a membrane-anchored probe array and an avidin-alkaline phosphatase (Av-AP) indicator system. The lower limit of detection of PCV using the miniarray was 101.9 tissue culture infectious dose 50 (TCID50)/ml and 102.08TCID50/ml for PCV1 and PCV2, respectively, and 100 viral copies/μl for both PCV1 and PCV2. We validated the miniarray system using 141 lymph node specimens from pigs with suspected postweaning multisystemic wasting syndrome or porcine dermatitis and nephropathy syndrome. Of the 141 samples evaluated, 55 were identified as positive for PCV by the miniarray. Relative to in situ hybridization, the sensitivity and specificity of the miniarray was 100% and 98.9%, respectively. In contrast to other microarray systems, the miniarray does not require a DNA chip reader, since the results can be determined by visual inspection of colorized spots on a nylon membrane. This system represents an effective alternative method for the differential detection of PCV1 and PCV2 in pigs, as well as the maintenance of PCV-free cell lines and pre-screening of commercial vaccines for possible contamination.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Miniarray</term></item><item><term>PCV2</term></item><item><term>PCV1</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Medicine & Public Health</head><item><term>Zoology</term></item><item><term>Veterinary Medicine</term></item></list></keywords></textClass></profileDesc><revisionDesc><change 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Article</ArticleCategory><ArticleFirstPage>139</ArticleFirstPage><ArticleLastPage>147</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2008</Year><Month>7</Month><Day>4</Day></RegistrationDate><Accepted><Year>2008</Year><Month>7</Month><Day>3</Day></Accepted><OnlineDate><Year>2008</Year><Month>7</Month><Day>24</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>2008</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>D.</GivenName><GivenName>J.</GivenName><FamilyName>An</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>D.</GivenName><GivenName>S.</GivenName><FamilyName>Song</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>J.</GivenName><GivenName>Y.</GivenName><FamilyName>Park</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3" CorrespondingAffiliationID="Aff3"><AuthorName DisplayOrder="Western"><GivenName>B.</GivenName><GivenName>K.</GivenName><FamilyName>Park</FamilyName></AuthorName><Contact><Email>parkx026@snu.ac.kr</Email></Contact></Author><Affiliation ID="Aff1"><OrgName>National Veterinary Research and Quarantine Service</OrgName><OrgAddress><City>Anyang</City><Street>Kyunggi-do</Street><Postcode>430–824</Postcode><Country Code="KR">Korea</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Research Unit</OrgDivision><OrgName>Green Cross Veterinary Products</OrgName><OrgAddress><City>Yong-in</City><Postcode>449–903</Postcode><Country Code="KR">Korea</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>College of Veterinary Medicine and BK21 Program for Veterinary Science</OrgDivision><OrgName>Seoul National University</OrgName><OrgAddress><City>Seoul</City><Postcode>151–742</Postcode><Country Code="KR">Korea</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>A miniarray system was developed for the simultaneous detection of porcine circovirus type 1 (PCV1) and type 2 (PCV2) in pigs. The system consists of a polymerase chain reaction (PCR) step to amplify target viral DNA, followed by detection of the amplified DNA using a membrane-anchored probe array and an avidin-alkaline phosphatase (Av-AP) indicator system. The lower limit of detection of PCV using the miniarray was 10<Superscript>1.9</Superscript> tissue culture infectious dose 50 (TCID<Subscript>50</Subscript>)/ml and 10<Superscript>2.08</Superscript>TCID<Subscript>50</Subscript>/ml for PCV1 and PCV2, respectively, and 100 viral copies/μl for both PCV1 and PCV2. We validated the miniarray system using 141 lymph node specimens from pigs with suspected postweaning multisystemic wasting syndrome or porcine dermatitis and nephropathy syndrome. Of the 141 samples evaluated, 55 were identified as positive for PCV by the miniarray. Relative to <Emphasis Type="Italic">in situ</Emphasis> hybridization, the sensitivity and specificity of the miniarray was 100% and 98.9%, respectively. In contrast to other microarray systems, the miniarray does not require a DNA chip reader, since the results can be determined by visual inspection of colorized spots on a nylon membrane. This system represents an effective alternative method for the differential detection of PCV1 and PCV2 in pigs, as well as the maintenance of PCV-free cell lines and pre-screening of commercial vaccines for possible contamination.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>Miniarray</Keyword><Keyword>PCV2</Keyword><Keyword>PCV1</Keyword></KeywordGroup><AbbreviationGroup><AbbreviationGroupSection ID="AGS1"><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term>PCV1</Term><Description><Para>porcine circovirus type 1</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PCV2</Term><Description><Para>porcine circovirus type 2</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PMWS</Term><Description><Para>postweaning multisystemic wasting syndrome</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PDNS</Term><Description><Para>porcine dermatitis and nephropathy syndrome</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PCVAD</Term><Description><Para>porcine circovirus associated disease</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroupSection></AbbreviationGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>A DNA miniarray system for simultaneous visual detection of porcine circovirus type 1 (PCV1) and 2 (PCV2) in pigs</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>A DNA miniarray system for simultaneous visual detection of porcine circovirus type 1 (PCV1) and 2 (PCV2) in pigs</title></titleInfo><name type="personal"><namePart type="given">D.</namePart><namePart type="given">J.</namePart><namePart type="family">An</namePart><affiliation>National Veterinary Research and Quarantine Service, Kyunggi-do, 430–824, Anyang, Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="given">S.</namePart><namePart type="family">Song</namePart><affiliation>Research Unit, Green Cross Veterinary Products, 449–903, Yong-in, Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="given">Y.</namePart><namePart type="family">Park</namePart><affiliation>National Veterinary Research and Quarantine Service, Kyunggi-do, 430–824, Anyang, Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">B.</namePart><namePart type="given">K.</namePart><namePart type="family">Park</namePart><affiliation>College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, 151–742, Seoul, Korea</affiliation><affiliation>E-mail: parkx026@snu.ac.kr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Netherlands</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateIssued encoding="w3cdtf">2009-02-01</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: A miniarray system was developed for the simultaneous detection of porcine circovirus type 1 (PCV1) and type 2 (PCV2) in pigs. The system consists of a polymerase chain reaction (PCR) step to amplify target viral DNA, followed by detection of the amplified DNA using a membrane-anchored probe array and an avidin-alkaline phosphatase (Av-AP) indicator system. The lower limit of detection of PCV using the miniarray was 101.9 tissue culture infectious dose 50 (TCID50)/ml and 102.08TCID50/ml for PCV1 and PCV2, respectively, and 100 viral copies/μl for both PCV1 and PCV2. We validated the miniarray system using 141 lymph node specimens from pigs with suspected postweaning multisystemic wasting syndrome or porcine dermatitis and nephropathy syndrome. Of the 141 samples evaluated, 55 were identified as positive for PCV by the miniarray. Relative to in situ hybridization, the sensitivity and specificity of the miniarray was 100% and 98.9%, respectively. In contrast to other microarray systems, the miniarray does not require a DNA chip reader, since the results can be determined by visual inspection of colorized spots on a nylon membrane. This system represents an effective alternative method for the differential detection of PCV1 and PCV2 in pigs, as well as the maintenance of PCV-free cell lines and pre-screening of commercial vaccines for possible contamination.</abstract><note>Original Article</note><subject lang="en"><genre>Keywords</genre><topic>Miniarray</topic><topic>PCV2</topic><topic>PCV1</topic></subject><subject></subject><relatedItem type="host"><titleInfo><title>Veterinary Research Communications</title><subTitle>An International Journal Publishing Topical Reviews and Research Articles on all Aspects of the Veterinary Sciences</subTitle></titleInfo><titleInfo type="abbreviated"><title>Vet Res Commun</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2009-01-20</dateIssued><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><subject><genre>Medicine & Public Health</genre><topic>Zoology</topic><topic>Veterinary Medicine</topic></subject><identifier type="ISSN">0165-7380</identifier><identifier type="eISSN">1573-7446</identifier><identifier type="JournalID">11259</identifier><identifier type="IssueArticleCount">9</identifier><identifier type="VolumeIssueCount">8</identifier><part><date>2009</date><detail type="volume"><number>33</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="pages"><start>139</start><end>147</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media B.V., 2009</recordOrigin></recordInfo></relatedItem><identifier type="istex">F3B33621E228B8E5610F13090FE4CC356F110B97</identifier><identifier type="ark">ark:/67375/VQC-6FRD0T5R-S</identifier><identifier type="DOI">10.1007/s11259-008-9080-8</identifier><identifier type="ArticleID">9080</identifier><identifier type="ArticleID">s11259-008-9080-8</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media B.V., 2008</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media B.V., 2008</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-6FRD0T5R-S/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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