Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA
Identifieur interne : 000F46 ( Istex/Corpus ); précédent : 000F45; suivant : 000F47Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA
Auteurs : Serge Cosnier ; Rodica E. Ionescu ; Sebastien Herrmann ; Laurent Bouffier ; Martine Demeunynck ; Robert S. MarksSource :
- Analytical Chemistry [ 0003-2700 ] ; 2006.
Abstract
The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.
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DOI: 10.1021/ac060926a
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E-mail:serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</mods:affiliation></affiliation></author><author><name sortKey="Ionescu, Rodica E" sort="Ionescu, Rodica E" uniqKey="Ionescu R" first="Rodica E." last="Ionescu">Rodica E. Ionescu</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> LEOPR, Université Joseph Fourier.</mods:affiliation></affiliation></author><author><name sortKey="Herrmann, Sebastien" sort="Herrmann, Sebastien" uniqKey="Herrmann S" first="Sebastien" last="Herrmann">Sebastien Herrmann</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> Ben-Gurion University of the Negev.</mods:affiliation></affiliation></author><author><name sortKey="Bouffier, Laurent" sort="Bouffier, Laurent" uniqKey="Bouffier L" first="Laurent" last="Bouffier">Laurent Bouffier</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> LEOPR, Université Joseph Fourier.</mods:affiliation></affiliation><affiliation><mods:affiliation> LEDSS, Université Joseph Fourier.</mods:affiliation></affiliation></author><author><name sortKey="Demeunynck, Martine" sort="Demeunynck, Martine" uniqKey="Demeunynck M" first="Martine" last="Demeunynck">Martine Demeunynck</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> LEDSS, Université Joseph Fourier.</mods:affiliation></affiliation></author><author><name sortKey="Marks, Robert S" sort="Marks, Robert S" uniqKey="Marks R" first="Robert S." last="Marks">Robert S. Marks</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> Ben-Gurion University of the Negev.</mods:affiliation></affiliation><affiliation><mods:affiliation> Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail:serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:5F3B21B41EEBA49750368605F44AD8BE47734942</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1021/ac060926a</idno><idno type="url">https://api.istex.fr/ark:/67375/TPS-XWTCHZGD-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000F46</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F46</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Electroenzymatic Polypyrrole-intercalator Sensor
for the Determination of West Nile Virus cDNA</title><author><name sortKey="Cosnier, Serge" sort="Cosnier, Serge" uniqKey="Cosnier S" first="Serge" last="Cosnier">Serge Cosnier</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> LEOPR, Université Joseph Fourier.</mods:affiliation></affiliation><affiliation><mods:affiliation> Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail:serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</mods:affiliation></affiliation></author><author><name sortKey="Ionescu, Rodica E" sort="Ionescu, Rodica E" uniqKey="Ionescu R" first="Rodica E." last="Ionescu">Rodica E. Ionescu</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> LEOPR, Université Joseph Fourier.</mods:affiliation></affiliation></author><author><name sortKey="Herrmann, Sebastien" sort="Herrmann, Sebastien" uniqKey="Herrmann S" first="Sebastien" last="Herrmann">Sebastien Herrmann</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> Ben-Gurion University of the Negev.</mods:affiliation></affiliation></author><author><name sortKey="Bouffier, Laurent" sort="Bouffier, Laurent" uniqKey="Bouffier L" first="Laurent" last="Bouffier">Laurent Bouffier</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> LEOPR, Université Joseph Fourier.</mods:affiliation></affiliation><affiliation><mods:affiliation> LEDSS, Université Joseph Fourier.</mods:affiliation></affiliation></author><author><name sortKey="Demeunynck, Martine" sort="Demeunynck, Martine" uniqKey="Demeunynck M" first="Martine" last="Demeunynck">Martine Demeunynck</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> LEDSS, Université Joseph Fourier.</mods:affiliation></affiliation></author><author><name sortKey="Marks, Robert S" sort="Marks, Robert S" uniqKey="Marks R" first="Robert S." last="Marks">Robert S. Marks</name><affiliation><mods:affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation> Ben-Gurion University of the Negev.</mods:affiliation></affiliation><affiliation><mods:affiliation> Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail:serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Analytical Chemistry</title><title level="j" type="abbrev">Anal. Chem.</title><idno type="ISSN">0003-2700</idno><idno type="eISSN">1520-6882</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2006</date><date type="published">2006</date><biblScope unit="vol">78</biblScope><biblScope unit="issue">19</biblScope><biblScope unit="page" from="7054">7054</biblScope><biblScope unit="page" to="7057">7057</biblScope></imprint><idno type="ISSN">0003-2700</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2700</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.</div></front></TEI><istex><corpusName>acs</corpusName><keywords><teeft><json:string>hybridization</json:string><json:string>electrochemical</json:string><json:string>dsdna</json:string><json:string>glucose</json:string><json:string>oligonucleotide</json:string><json:string>chem</json:string><json:string>biotinylated</json:string><json:string>amperometric</json:string><json:string>intercalator</json:string><json:string>polypyrrole</json:string><json:string>current response</json:string><json:string>target concentration</json:string><json:string>electrode surface</json:string><json:string>duplex</json:string><json:string>joseph fourier</json:string><json:string>analytical chemistry</json:string><json:string>room temperature</json:string><json:string>redox acridone derivative</json:string><json:string>citrate buffer</json:string><json:string>hybridization event</json:string><json:string>cyclic voltammogram</json:string><json:string>reversible peak system</json:string><json:string>scan rate</json:string><json:string>current intensity</json:string><json:string>electrode</json:string><json:string>sensor</json:string><json:string>phosphate buffer</json:string><json:string>various target concentration</json:string><json:string>grenoble cedex</json:string><json:string>covalent binding</json:string><json:string>fishing step</json:string><json:string>affinity interaction</json:string><json:string>hybridization process</json:string><json:string>genomic sensor</json:string><json:string>apparent surface coverage</json:string><json:string>polypyrrole electroactivity</json:string><json:string>cyclic voltammograms</json:string><json:string>amperometric response</json:string><json:string>electrochemical oxidation</json:string><json:string>poly1 film</json:string><json:string>amperometric detection</json:string><json:string>reference electrode</json:string><json:string>peak system</json:string><json:string>electrochemical signal</json:string><json:string>biotinylated complementary oligonucleotide</json:string><json:string>hybridization buffer</json:string></teeft></keywords><author><json:item><name>COSNIER Serge</name><affiliations><json:string>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</json:string><json:string>LEOPR, Université Joseph Fourier.</json:string><json:string>Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail:serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</json:string></affiliations></json:item><json:item><name>IONESCU Rodica E.</name><affiliations><json:string>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</json:string><json:string>LEOPR, Université Joseph Fourier.</json:string></affiliations></json:item><json:item><name>HERRMANN Sebastien</name><affiliations><json:string>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</json:string><json:string>Ben-Gurion University of the Negev.</json:string></affiliations></json:item><json:item><name>BOUFFIER Laurent</name><affiliations><json:string>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</json:string><json:string>LEOPR, Université Joseph Fourier.</json:string><json:string>LEDSS, Université Joseph Fourier.</json:string></affiliations></json:item><json:item><name>DEMEUNYNCK Martine</name><affiliations><json:string>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</json:string><json:string>LEDSS, Université Joseph Fourier.</json:string></affiliations></json:item><json:item><name>MARKS Robert S.</name><affiliations><json:string>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</json:string><json:string>Ben-Gurion University of the Negev.</json:string><json:string>Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail:serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</json:string></affiliations></json:item></author><arkIstex>ark:/67375/TPS-XWTCHZGD-F</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article-commentary</json:string></originalGenre><abstract>The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. 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for the Determination of West Nile Virus cDNA</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>American Chemical Society</publisher><availability><licence>Copyright © 2006 American Chemical Society</licence><p>American Chemical Society</p></availability><date type="published">2006</date><date type="Copyright" when="2006">2006</date></publicationStmt><notesStmt><note type="content-type" source="article-commentary" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-L5L7X3NF-P">review-article</note><note type="publication-type" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Electroenzymatic Polypyrrole-intercalator Sensor
for the Determination of West Nile Virus cDNA</title><author xml:id="author-0000" role="corresp"><persName><surname>Cosnier</surname><forename type="first">Serge</forename></persName><note place="foot" n="ac060926aAF2"><ref>†</ref><p>
LEOPR, Université Joseph Fourier.</p></note><affiliation role="corresp"> Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail: serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</affiliation></author><author xml:id="author-0001"><persName><surname>Ionescu</surname><forename type="first">Rodica E.</forename></persName><affiliation><orgName type="institution">LEOPR (CNRS UMR 5630)</orgName><orgName type="institution">Institut de Chimie Moléculaire de Grenoble FR CNRS 2607</orgName><orgName type="institution">Université Joseph Fourier</orgName><address><addrLine>BP 53</addrLine><addrLine>38041 Grenoble Cedex 9</addrLine><country key="FR" xml:lang="en">FRANCE</country><addrLine>Department of Biotechnology Engineering and National Institute for Biotechnology in the Negev</addrLine><addrLine>Ben-Gurion University of the Negev</addrLine><addrLine>P.O. Box 653</addrLine><addrLine>84105 Beer-Sheva</addrLine><country key="IL" xml:lang="en">ISRAEL</country><addrLine>and LEDSS UMR 5616 & ICMG-FR 2607</addrLine><addrLine>Université Joseph Fourier</addrLine><addrLine>BP 53</addrLine><addrLine>38041 Grenoble Cedex 9</addrLine><country key="FR" xml:lang="en">FRANCE</country></address></affiliation><note place="foot" n="ac060926aAF2"><ref>†</ref><p>
LEOPR, Université Joseph Fourier.</p></note></author><author xml:id="author-0002"><persName><surname>Herrmann</surname><forename type="first">Sebastien</forename></persName><affiliation><orgName type="institution">LEOPR (CNRS UMR 5630)</orgName><orgName type="institution">Institut de Chimie Moléculaire de Grenoble FR CNRS 2607</orgName><orgName type="institution">Université Joseph Fourier</orgName><address><addrLine>BP 53</addrLine><addrLine>38041 Grenoble Cedex 9</addrLine><country key="FR" xml:lang="en">FRANCE</country><addrLine>Department of Biotechnology Engineering and National Institute for Biotechnology in the Negev</addrLine><addrLine>Ben-Gurion University of the Negev</addrLine><addrLine>P.O. Box 653</addrLine><addrLine>84105 Beer-Sheva</addrLine><country key="IL" xml:lang="en">ISRAEL</country><addrLine>and LEDSS UMR 5616 & ICMG-FR 2607</addrLine><addrLine>Université Joseph Fourier</addrLine><addrLine>BP 53</addrLine><addrLine>38041 Grenoble Cedex 9</addrLine><country key="FR" xml:lang="en">FRANCE</country></address></affiliation><note place="foot" n="ac060926aAF3"><ref>‡</ref><p>
Ben-Gurion University of the Negev.</p></note></author><author xml:id="author-0003"><persName><surname>Bouffier</surname><forename type="first">Laurent</forename></persName><note place="foot" n="ac060926aAF2"><ref>†</ref><p>
LEOPR, Université Joseph Fourier.</p></note><note place="foot" n="ac060926aAF4"><ref>§</ref><p>
LEDSS, Université Joseph Fourier.</p></note></author><author xml:id="author-0004"><persName><surname>Demeunynck</surname><forename type="first">Martine</forename></persName><affiliation><orgName type="institution">LEOPR (CNRS UMR 5630)</orgName><orgName type="institution">Institut de Chimie Moléculaire de Grenoble FR CNRS 2607</orgName><orgName type="institution">Université Joseph Fourier</orgName><address><addrLine>BP 53</addrLine><addrLine>38041 Grenoble Cedex 9</addrLine><country key="FR" xml:lang="en">FRANCE</country><addrLine>Department of Biotechnology Engineering and National Institute for Biotechnology in the Negev</addrLine><addrLine>Ben-Gurion University of the Negev</addrLine><addrLine>P.O. Box 653</addrLine><addrLine>84105 Beer-Sheva</addrLine><country key="IL" xml:lang="en">ISRAEL</country><addrLine>and LEDSS UMR 5616 & ICMG-FR 2607</addrLine><addrLine>Université Joseph Fourier</addrLine><addrLine>BP 53</addrLine><addrLine>38041 Grenoble Cedex 9</addrLine><country key="FR" xml:lang="en">FRANCE</country></address></affiliation><note place="foot" n="ac060926aAF4"><ref>§</ref><p>
LEDSS, Université Joseph Fourier.</p></note></author><author xml:id="author-0005" role="corresp"><persName><surname>Marks</surname><forename type="first">Robert S.</forename></persName><note place="foot" n="ac060926aAF3"><ref>‡</ref><p>
Ben-Gurion University of the Negev.</p></note><affiliation role="corresp"> Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail: serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</affiliation></author><idno type="istex">5F3B21B41EEBA49750368605F44AD8BE47734942</idno><idno type="ark">ark:/67375/TPS-XWTCHZGD-F</idno><idno type="DOI">10.1021/ac060926a</idno></analytic><monogr><title level="j" type="main">Analytical Chemistry</title><title level="j" type="abbrev">Anal. Chem.</title><idno type="acspubs">ac</idno><idno type="coden">ancham</idno><idno type="pISSN">0003-2700</idno><idno type="eISSN">1520-6882</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2006</date><date type="published">2006</date><biblScope unit="vol">78</biblScope><biblScope unit="issue">19</biblScope><biblScope unit="page" from="7054">7054</biblScope><biblScope unit="page" to="7057">7057</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><encodingDesc><schemaRef type="ODD" url="https://xml-schema.delivery.istex.fr/tei-istex.odd"></schemaRef><appInfo><application ident="pub2tei" version="1.0.41" when="2020-04-06"><label>pub2TEI-ISTEX</label><desc>A set of style sheets for converting XML documents encoded in various scientific publisher formats into a common TEI format.
<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract><p>The chemical binding of a redox acridone derivative onto
a polypyrrole film functionalized by <hi rend="italic">N</hi>-hydroxysuccinimide
groups provided an electrode capable of anchoring DNA
duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was
applied for the detection of a ssDNA derived from a West
Nile virus sequence. The latter was thus amperometrically
detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.
</p></abstract><textClass ana="subject"><keywords scheme="document-type-name"><term>Small Correspondence</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2020-04-06" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="corpus acs not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:document><article article-type="article-commentary" specific-use="acs2jats-2.0.3"><front><journal-meta><journal-id journal-id-type="acspubs">ac</journal-id><journal-id journal-id-type="coden">ancham</journal-id><journal-title-group><journal-title>Analytical Chemistry</journal-title><abbrev-journal-title>Anal. Chem.</abbrev-journal-title></journal-title-group><issn pub-type="ppub">0003-2700</issn><issn pub-type="epub">1520-6882</issn><publisher><publisher-name>American Chemical Society</publisher-name></publisher><self-uri>pubs.acs.org/ac</self-uri></journal-meta><article-meta><article-id pub-id-type="doi">10.1021/ac060926a</article-id><article-categories><subj-group subj-group-type="document-type-name"><subject>Small Correspondence</subject></subj-group></article-categories><title-group><article-title>Electroenzymatic Polypyrrole-intercalator Sensor
for the Determination of West Nile Virus cDNA</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Cosnier</surname><given-names>Serge</given-names></name><xref rid="ac060926aAF1">*</xref><xref rid="ac060926aAF2">†</xref></contrib><contrib contrib-type="author"><name><surname>Ionescu</surname><given-names>Rodica E.</given-names></name><xref rid="ac060926aAF2">†</xref></contrib><contrib contrib-type="author"><name><surname>Herrmann</surname><given-names>Sebastien</given-names></name><xref rid="ac060926aAF3">‡</xref></contrib><contrib contrib-type="author"><name><surname>Bouffier</surname><given-names>Laurent</given-names></name><xref rid="ac060926aAF2">†</xref><xref rid="ac060926aAF4">§</xref></contrib><contrib contrib-type="author"><name><surname>Demeunynck</surname><given-names>Martine</given-names></name><xref rid="ac060926aAF4">§</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Marks</surname><given-names>Robert S.</given-names></name><xref rid="ac060926aAF1">*</xref><xref rid="ac060926aAF3">‡</xref></contrib><aff>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,
38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in the
Negev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR
2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France
</aff></contrib-group><author-notes><corresp id="ac060926aAF1">
Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail:
serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</corresp><fn id="ac060926aAF2"><label>†</label><p>
LEOPR, Université Joseph Fourier.</p></fn><fn id="ac060926aAF3"><label>‡</label><p>
Ben-Gurion University of the Negev.</p></fn><fn id="ac060926aAF4"><label>§</label><p>
LEDSS, Université Joseph Fourier.</p></fn></author-notes><pub-date pub-type="epub"><day>29</day><month>08</month><year>2006</year></pub-date><pub-date pub-type="ppub"><day>01</day><month>10</month><year>2006</year></pub-date><volume>78</volume><issue>19</issue><fpage>7054</fpage><lpage>7057</lpage><history><date date-type="received"><day>19</day><month>05</month><year>2006</year></date><date date-type="asap"><day>29</day><month>08</month><year>2006</year></date><date date-type="issue-pub"><day>01</day><month>10</month><year>2006</year></date></history><permissions><copyright-statement>Copyright © 2006 American Chemical Society</copyright-statement><copyright-year>2006</copyright-year><copyright-holder>American Chemical Society</copyright-holder></permissions><abstract><p>The chemical binding of a redox acridone derivative onto
a polypyrrole film functionalized by <italic>N</italic>-hydroxysuccinimide
groups provided an electrode capable of anchoring DNA
duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was
applied for the detection of a ssDNA derived from a West
Nile virus sequence. The latter was thus amperometrically
detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.
</p></abstract><custom-meta-group><custom-meta><meta-name>document-id-old-9</meta-name><meta-value>ac060926a</meta-value></custom-meta></custom-meta-group></article-meta></front><body></body><back></back></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA</title></titleInfo><titleInfo contentType="CDATA"><title>Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="family">COSNIER</namePart><namePart type="given">Serge</namePart><affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</affiliation><affiliation> LEOPR, Université Joseph Fourier.</affiliation><affiliation> Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail:serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="family">IONESCU</namePart><namePart type="given">Rodica E.</namePart><affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</affiliation><affiliation> LEOPR, Université Joseph Fourier.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="family">HERRMANN</namePart><namePart type="given">Sebastien</namePart><affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</affiliation><affiliation> Ben-Gurion University of the Negev.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="family">BOUFFIER</namePart><namePart type="given">Laurent</namePart><affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</affiliation><affiliation> LEOPR, Université Joseph Fourier.</affiliation><affiliation> LEDSS, Université Joseph Fourier.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="family">DEMEUNYNCK</namePart><namePart type="given">Martine</namePart><affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</affiliation><affiliation> LEDSS, Université Joseph Fourier.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="family">MARKS</namePart><namePart type="given">Robert S.</namePart><affiliation>LEOPR (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53,38041 Grenoble Cedex 9, France, Department of Biotechnology Engineering and National Institute for Biotechnology in theNegev, Ben-Gurion University of the Negev, P.O. Box 653, 84105 Beer-Sheva, Israel, and LEDSS UMR 5616 & ICMG-FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France</affiliation><affiliation> Ben-Gurion University of the Negev.</affiliation><affiliation> Corresponding authors. Tel: 33 4 76 51 49 98. Fax: 33 4 76 51 42 67. E-mail:serge.cosnier@ujf-grenoble.fr and rsmarks@bgumail.bgu.ac.il.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="other" displayLabel="article-commentary" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-7474895G-0">other</genre><originInfo><publisher>American Chemical Society</publisher><dateCreated encoding="w3cdtf">2006-08-29</dateCreated><dateIssued encoding="w3cdtf">2006-10-01</dateIssued><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract>The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.</abstract><relatedItem type="host"><titleInfo><title>Analytical Chemistry</title></titleInfo><titleInfo type="abbreviated"><title>Anal. 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