Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure
Identifieur interne : 000E24 ( Istex/Corpus ); précédent : 000E23; suivant : 000E25Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure
Auteurs : Zhihui Xu ; Xiaoqiang Ren ; Yan Liu ; Xiaodong Li ; Siyu Bai ; Yanwei Zhong ; Lin Wang ; Panyong Mao ; Huifen Wang ; Shaojie Xin ; Vincent Wai-Sun Wong ; Henry Lik-Yuen Chan ; Fabien Zoulim ; Dongping XuSource :
- Journal of Gastroenterology [ 0944-1174 ] ; 2011-03-01.
English descriptors
- KwdEn :
Abstract
Abstract: Objective: To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features. Patients and methods: Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) patients admitted to or who had visited Beijing 302 Hospital from January 2005 to December 2008 were collected and successfully amplified for the HBV BCP/PC and a 1225-bp-long S/Pol (nt 54–1278) gene regions. Biochemical and serological parameters and HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested PCR. Genotypes/subgenotypes were determined based on complete genomic sequence or on analysis of the 1225-bp-long S/Pol-gene sequence. HBV genotyping was performed by direct PCR sequencing followed by molecular evolutionary analysis of the viral sequences. A P value of <0.05 (two-sided) was considered to be statistically significant. Conclusions: Our findings suggest that CHB patients infected with BCP/PC mutant viruses are more susceptible to severe hepatitis and ACLF than those with the BCP/PC wild-type virus and that ACLF patients with PC mutant viruses have an increased risk of death. As such, the HBV PC mutation is a potential predictive indicator of ACLF outcome.
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DOI: 10.1007/s00535-010-0315-4
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China</mods:affiliation></affiliation></author><author><name sortKey="Chan, Henry Lik Yuen" sort="Chan, Henry Lik Yuen" uniqKey="Chan H" first="Henry Lik-Yuen" last="Chan">Henry Lik-Yuen Chan</name><affiliation><mods:affiliation>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</mods:affiliation></affiliation></author><author><name sortKey="Zoulim, Fabien" sort="Zoulim, Fabien" uniqKey="Zoulim F" first="Fabien" last="Zoulim">Fabien Zoulim</name><affiliation><mods:affiliation>INSERM, U871, 69003, Lyon, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Hepatology and Gastroenterology, Hospices Civils de Lyon, Hôtel Dieu Hospital, 69002, Lyon, France</mods:affiliation></affiliation></author><author><name sortKey="Xu, Dongping" sort="Xu, Dongping" uniqKey="Xu D" first="Dongping" last="Xu">Dongping Xu</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, 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liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure</title><author><name sortKey="Xu, Zhihui" sort="Xu, Zhihui" uniqKey="Xu Z" first="Zhihui" last="Xu">Zhihui Xu</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Ren, Xiaoqiang" sort="Ren, Xiaoqiang" uniqKey="Ren X" first="Xiaoqiang" last="Ren">Xiaoqiang Ren</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Li, Xiaodong" sort="Li, Xiaodong" uniqKey="Li X" first="Xiaodong" last="Li">Xiaodong Li</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Bai, Siyu" sort="Bai, Siyu" uniqKey="Bai S" first="Siyu" last="Bai">Siyu Bai</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Zhong, Yanwei" sort="Zhong, Yanwei" uniqKey="Zhong Y" first="Yanwei" last="Zhong">Yanwei Zhong</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Mao, Panyong" sort="Mao, Panyong" uniqKey="Mao P" first="Panyong" last="Mao">Panyong Mao</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Wang, Huifen" sort="Wang, Huifen" uniqKey="Wang H" first="Huifen" last="Wang">Huifen Wang</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Xin, Shaojie" sort="Xin, Shaojie" uniqKey="Xin S" first="Shaojie" last="Xin">Shaojie Xin</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Wong, Vincent Wai Sun" sort="Wong, Vincent Wai Sun" uniqKey="Wong V" first="Vincent Wai-Sun" last="Wong">Vincent Wai-Sun Wong</name><affiliation><mods:affiliation>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</mods:affiliation></affiliation></author><author><name sortKey="Chan, Henry Lik Yuen" sort="Chan, Henry Lik Yuen" uniqKey="Chan H" first="Henry Lik-Yuen" last="Chan">Henry Lik-Yuen Chan</name><affiliation><mods:affiliation>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</mods:affiliation></affiliation></author><author><name sortKey="Zoulim, Fabien" sort="Zoulim, Fabien" uniqKey="Zoulim F" first="Fabien" last="Zoulim">Fabien Zoulim</name><affiliation><mods:affiliation>INSERM, U871, 69003, Lyon, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Hepatology and Gastroenterology, Hospices Civils de Lyon, Hôtel Dieu Hospital, 69002, Lyon, France</mods:affiliation></affiliation></author><author><name sortKey="Xu, Dongping" sort="Xu, Dongping" uniqKey="Xu D" first="Dongping" last="Xu">Dongping Xu</name><affiliation><mods:affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: xudongping@yahoo.com</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Gastroenterology</title><title level="j" type="abbrev">J Gastroenterol</title><idno type="ISSN">0944-1174</idno><idno type="eISSN">1435-5922</idno><imprint><publisher>Springer Japan</publisher><pubPlace>Japan</pubPlace><date type="published" when="2011-03-01">2011-03-01</date><biblScope unit="volume">46</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="391">391</biblScope><biblScope unit="page" to="400">400</biblScope></imprint><idno type="ISSN">0944-1174</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0944-1174</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute-on-chronic liver failure</term><term>Basal core promoter mutation</term><term>Genotype</term><term>Hepatitis B virus</term><term>Precore mutation</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Objective: To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features. Patients and methods: Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) patients admitted to or who had visited Beijing 302 Hospital from January 2005 to December 2008 were collected and successfully amplified for the HBV BCP/PC and a 1225-bp-long S/Pol (nt 54–1278) gene regions. Biochemical and serological parameters and HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested PCR. Genotypes/subgenotypes were determined based on complete genomic sequence or on analysis of the 1225-bp-long S/Pol-gene sequence. HBV genotyping was performed by direct PCR sequencing followed by molecular evolutionary analysis of the viral sequences. A P value of <0.05 (two-sided) was considered to be statistically significant. Conclusions: Our findings suggest that CHB patients infected with BCP/PC mutant viruses are more susceptible to severe hepatitis and ACLF than those with the BCP/PC wild-type virus and that ACLF patients with PC mutant viruses have an increased risk of death. As such, the HBV PC mutation is a potential predictive indicator of ACLF outcome.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Zhihui Xu</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Xiaoqiang Ren</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Yan Liu</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Xiaodong Li</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Siyu Bai</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Yanwei Zhong</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Lin Wang</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Panyong Mao</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Huifen Wang</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Shaojie Xin</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string></affiliations></json:item><json:item><name>Vincent Wai-Sun Wong</name><affiliations><json:string>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</json:string></affiliations></json:item><json:item><name>Henry Lik-Yuen Chan</name><affiliations><json:string>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</json:string></affiliations></json:item><json:item><name>Fabien Zoulim</name><affiliations><json:string>INSERM, U871, 69003, Lyon, France</json:string><json:string>Department of Hepatology and Gastroenterology, Hospices Civils de Lyon, Hôtel Dieu Hospital, 69002, Lyon, France</json:string></affiliations></json:item><json:item><name>Dongping Xu</name><affiliations><json:string>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</json:string><json:string>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</json:string><json:string>E-mail: xudongping@yahoo.com</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Hepatitis B virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Basal core promoter mutation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Precore mutation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Genotype</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Acute-on-chronic liver failure</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ACLF : Acute-on-chronic liver failure</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ALF : Acute liver failure</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ALT : Alanine aminotransferase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>BCP : Basal core promoter</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CHB-M : Mild chronic hepatitis B</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CHB-S : Severe chronic hepatitis B</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HBV : Hepatitis B virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HCC : Hepatocellular carcinoma</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LC : Liver cirrhosis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PC : Precore</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PTA : Prothrombin activity</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>TBIL : Total bilirubin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCR : Polymerase chain reaction</value></json:item></subject><articleId><json:string>315</json:string><json:string>s00535-010-0315-4</json:string></articleId><arkIstex>ark:/67375/VQC-RQ7N7HGC-P</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Objective: To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features. Patients and methods: Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) patients admitted to or who had visited Beijing 302 Hospital from January 2005 to December 2008 were collected and successfully amplified for the HBV BCP/PC and a 1225-bp-long S/Pol (nt 54–1278) gene regions. Biochemical and serological parameters and HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested PCR. Genotypes/subgenotypes were determined based on complete genomic sequence or on analysis of the 1225-bp-long S/Pol-gene sequence. HBV genotyping was performed by direct PCR sequencing followed by molecular evolutionary analysis of the viral sequences. A P value of >0.05 (two-sided) was considered to be statistically significant. Conclusions: Our findings suggest that CHB patients infected with BCP/PC mutant viruses are more susceptible to severe hepatitis and ACLF than those with the BCP/PC wild-type virus and that ACLF patients with PC mutant viruses have an increased risk of death. As such, the HBV PC mutation is a potential predictive indicator of ACLF outcome.</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>210</abstractWordCount><abstractCharCount>1411</abstractCharCount><keywordCount>18</keywordCount><score>9.52</score><pdfWordCount>5867</pdfWordCount><pdfCharCount>38197</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>595.276 x 790.866 pts</pdfPageSize></qualityIndicators><title>Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure</title><genre><json:string>research-article</json:string></genre><host><title>Journal of Gastroenterology</title><language><json:string>unknown</json:string></language><publicationDate>2011</publicationDate><copyrightDate>2011</copyrightDate><issn><json:string>0944-1174</json:string></issn><eissn><json:string>1435-5922</json:string></eissn><journalId><json:string>535</json:string></journalId><volume>46</volume><issue>3</issue><pages><first>391</first><last>400</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Colorectal Surgery</value></json:item><json:item><value>Hepatology</value></json:item><json:item><value>Gastroenterology</value></json:item><json:item><value>Internal Medicine</value></json:item><json:item><value>Surgical 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disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Springer Japan</publisher><pubPlace>Japan</pubPlace><availability><licence><p>Springer, 2010</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>2010-05-11</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Original Article—Liver, Pancreas, and Biliary Tract</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure</title><author xml:id="author-0000"><persName><forename type="first">Zhihui</forename><surname>Xu</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Xiaoqiang</forename><surname>Ren</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Yan</forename><surname>Liu</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Xiaodong</forename><surname>Li</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Siyu</forename><surname>Bai</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Yanwei</forename><surname>Zhong</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0006"><persName><forename type="first">Lin</forename><surname>Wang</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0007"><persName><forename type="first">Panyong</forename><surname>Mao</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0008"><persName><forename type="first">Huifen</forename><surname>Wang</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0009"><persName><forename type="first">Shaojie</forename><surname>Xin</surname></persName><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><author xml:id="author-0010"><persName><forename type="first">Vincent</forename><surname>Wong</surname></persName><affiliation>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</affiliation></author><author xml:id="author-0011"><persName><forename type="first">Henry</forename><surname>Chan</surname></persName><affiliation>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</affiliation></author><author xml:id="author-0012"><persName><forename type="first">Fabien</forename><surname>Zoulim</surname></persName><affiliation>INSERM, U871, 69003, Lyon, France</affiliation><affiliation>Department of Hepatology and Gastroenterology, Hospices Civils de Lyon, Hôtel Dieu Hospital, 69002, Lyon, France</affiliation></author><author xml:id="author-0013" corresp="yes"><persName><forename type="first">Dongping</forename><surname>Xu</surname></persName><email>xudongping@yahoo.com</email><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation></author><idno type="istex">E0C7FCC069FEDE1A2C6F6010B6CB8895F3EF8BA0</idno><idno type="ark">ark:/67375/VQC-RQ7N7HGC-P</idno><idno type="DOI">10.1007/s00535-010-0315-4</idno><idno type="article-id">315</idno><idno type="article-id">s00535-010-0315-4</idno></analytic><monogr><title level="j">Journal of Gastroenterology</title><title level="j" type="abbrev">J Gastroenterol</title><idno type="pISSN">0944-1174</idno><idno type="eISSN">1435-5922</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">19</idno><idno type="volume-issue-count">12</idno><imprint><publisher>Springer Japan</publisher><pubPlace>Japan</pubPlace><date type="published" when="2011-03-01"></date><biblScope unit="volume">46</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="391">391</biblScope><biblScope unit="page" to="400">400</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010-05-11</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Objective: To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features. Patients and methods: Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) patients admitted to or who had visited Beijing 302 Hospital from January 2005 to December 2008 were collected and successfully amplified for the HBV BCP/PC and a 1225-bp-long S/Pol (nt 54–1278) gene regions. Biochemical and serological parameters and HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested PCR. Genotypes/subgenotypes were determined based on complete genomic sequence or on analysis of the 1225-bp-long S/Pol-gene sequence. HBV genotyping was performed by direct PCR sequencing followed by molecular evolutionary analysis of the viral sequences. A P value of <0.05 (two-sided) was considered to be statistically significant. Conclusions: Our findings suggest that CHB patients infected with BCP/PC mutant viruses are more susceptible to severe hepatitis and ACLF than those with the BCP/PC wild-type virus and that ACLF patients with PC mutant viruses have an increased risk of death. As such, the HBV PC mutation is a potential predictive indicator of ACLF outcome.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Hepatitis B virus</term></item><item><term>Basal core promoter mutation</term></item><item><term>Precore mutation</term></item><item><term>Genotype</term></item><item><term>Acute-on-chronic liver failure</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>ACLF</term><term>Acute-on-chronic liver failure</term></item><item><term>ALF</term><term>Acute liver failure</term></item><item><term>ALT</term><term>Alanine aminotransferase</term></item><item><term>BCP</term><term>Basal core promoter</term></item><item><term>CHB-M</term><term>Mild chronic hepatitis B</term></item><item><term>CHB-S</term><term>Severe chronic hepatitis B</term></item><item><term>HBV</term><term>Hepatitis B virus</term></item><item><term>HCC</term><term>Hepatocellular carcinoma</term></item><item><term>LC</term><term>Liver cirrhosis</term></item><item><term>PC</term><term>Precore</term></item><item><term>PTA</term><term>Prothrombin activity</term></item><item><term>TBIL</term><term>Total bilirubin</term></item><item><term>PCR</term><term>Polymerase chain reaction</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Medicine & Public Health</head><item><term>Colorectal Surgery</term></item><item><term>Hepatology</term></item><item><term>Gastroenterology</term></item><item><term>Internal Medicine</term></item><item><term>Surgical Oncology</term></item><item><term>Oncology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-05-11">Created</change><change when="2011-03-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-RQ7N7HGC-P/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer Japan</PublisherName><PublisherLocation>Japan</PublisherLocation></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="NonStandardArchiveJournal" NumberingStyle="Unnumbered"><JournalID>535</JournalID><JournalPrintISSN>0944-1174</JournalPrintISSN><JournalElectronicISSN>1435-5922</JournalElectronicISSN><JournalTitle>Journal of Gastroenterology</JournalTitle><JournalAbbreviatedTitle>J Gastroenterol</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Medicine & Public Health</JournalSubject><JournalSubject Type="Secondary">Colorectal Surgery</JournalSubject><JournalSubject Type="Secondary">Hepatology</JournalSubject><JournalSubject Type="Secondary">Gastroenterology</JournalSubject><JournalSubject Type="Secondary">Internal Medicine</JournalSubject><JournalSubject Type="Secondary">Surgical Oncology</JournalSubject><JournalSubject Type="Secondary">Oncology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>46</VolumeIDStart><VolumeIDEnd>46</VolumeIDEnd><VolumeIssueCount>12</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>3</IssueIDStart><IssueIDEnd>3</IssueIDEnd><IssueArticleCount>19</IssueArticleCount><IssueHistory><OnlineDate><Year>2011</Year><Month>3</Month><Day>31</Day></OnlineDate><PrintDate><Year>2011</Year><Month>3</Month><Day>30</Day></PrintDate><CoverDate><Year>2011</Year><Month>3</Month></CoverDate><PricelistYear>2011</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer</CopyrightHolderName><CopyrightYear>2011</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s00535-010-0315-4" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>315</ArticleID><ArticleDOI>10.1007/s00535-010-0315-4</ArticleDOI><ArticleSequenceNumber>13</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure</ArticleTitle><ArticleCategory>Original Article—Liver, Pancreas, and Biliary Tract</ArticleCategory><ArticleFirstPage>391</ArticleFirstPage><ArticleLastPage>400</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2010</Year><Month>8</Month><Day>17</Day></RegistrationDate><Received><Year>2010</Year><Month>5</Month><Day>11</Day></Received><Accepted><Year>2010</Year><Month>8</Month><Day>15</Day></Accepted><OnlineDate><Year>2010</Year><Month>9</Month><Day>17</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Zhihui</GivenName><FamilyName>Xu</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Xiaoqiang</GivenName><FamilyName>Ren</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Yan</GivenName><FamilyName>Liu</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Xiaodong</GivenName><FamilyName>Li</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Siyu</GivenName><FamilyName>Bai</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Yanwei</GivenName><FamilyName>Zhong</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Lin</GivenName><FamilyName>Wang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Panyong</GivenName><FamilyName>Mao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Huifen</GivenName><FamilyName>Wang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Shaojie</GivenName><FamilyName>Xin</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Vincent</GivenName><GivenName>Wai-Sun</GivenName><FamilyName>Wong</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Henry</GivenName><GivenName>Lik-Yuen</GivenName><FamilyName>Chan</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff4 Aff5"><AuthorName DisplayOrder="Western"><GivenName>Fabien</GivenName><FamilyName>Zoulim</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Dongping</GivenName><FamilyName>Xu</FamilyName></AuthorName><Contact><Phone>+86-10-63879286</Phone><Fax>+86-10-63879286</Fax><Email>xudongping@yahoo.com</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Viral Hepatitis Research Laboratory</OrgDivision><OrgName>Beijing Institute of Infectious Diseases</OrgName><OrgAddress><City>Beijing</City><Postcode>100039</Postcode><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Liver Failure Research Center</OrgDivision><OrgName>Beijing 302 Hospital</OrgName><OrgAddress><City>Beijing</City><Postcode>100039</Postcode><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Department of Medicine and Therapeutics and Institute of Digestive Disease</OrgDivision><OrgName>The Chinese University of Hong Kong</OrgName><OrgAddress><City>Hong Kong</City><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgName>INSERM, U871</OrgName><OrgAddress><Postcode>69003</Postcode><City>Lyon</City><Country Code="FR">France</Country></OrgAddress></Affiliation><Affiliation ID="Aff5"><OrgDivision>Department of Hepatology and Gastroenterology, Hospices Civils de Lyon</OrgDivision><OrgName>Hôtel Dieu Hospital</OrgName><OrgAddress><Postcode>69002</Postcode><City>Lyon</City><Country Code="FR">France</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><AbstractSection ID="ASec1"><Heading>Objective</Heading><Para>To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features.</Para></AbstractSection><AbstractSection ID="ASec2"><Heading>Patients and methods</Heading><Para>Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) patients admitted to or who had visited Beijing 302 Hospital from January 2005 to December 2008 were collected and successfully amplified for the HBV BCP/PC and a 1225-bp-long S/Pol (nt 54–1278) gene regions. Biochemical and serological parameters and HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested PCR. Genotypes/subgenotypes were determined based on complete genomic sequence or on analysis of the 1225-bp-long S/Pol-gene sequence. HBV genotyping was performed by direct PCR sequencing followed by molecular evolutionary analysis of the viral sequences. A <Emphasis Type="Italic">P</Emphasis> value of <0.05 (two-sided) was considered to be statistically significant.</Para></AbstractSection><AbstractSection ID="ASec4"><Heading>Conclusions</Heading><Para>Our findings suggest that CHB patients infected with BCP/PC mutant viruses are more susceptible to severe hepatitis and ACLF than those with the BCP/PC wild-type virus and that ACLF patients with PC mutant viruses have an increased risk of death. As such, the HBV PC mutation is a potential predictive indicator of ACLF outcome.</Para></AbstractSection></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Hepatitis B virus</Keyword><Keyword>Basal core promoter mutation</Keyword><Keyword>Precore mutation</Keyword><Keyword>Genotype</Keyword><Keyword>Acute-on-chronic liver failure</Keyword></KeywordGroup><AbbreviationGroup><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term>ACLF</Term><Description><Para>Acute-on-chronic liver failure</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>ALF</Term><Description><Para>Acute liver failure</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>ALT</Term><Description><Para>Alanine aminotransferase</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>BCP</Term><Description><Para>Basal core promoter</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>CHB-M</Term><Description><Para>Mild chronic hepatitis B</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>CHB-S</Term><Description><Para>Severe chronic hepatitis B</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>HBV</Term><Description><Para>Hepatitis B virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>HCC</Term><Description><Para>Hepatocellular carcinoma</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>LC</Term><Description><Para>Liver cirrhosis</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PC</Term><Description><Para>Precore</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PTA</Term><Description><Para>Prothrombin activity</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>TBIL</Term><Description><Para>Total bilirubin</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PCR</Term><Description><Para>Polymerase chain reaction</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroup><ArticleNote Type="Misc"><SimplePara>Z. Xu, X. Ren, and Y. Liu contributed equally to this work.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure</title></titleInfo><name type="personal"><namePart type="given">Zhihui</namePart><namePart type="family">Xu</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xiaoqiang</namePart><namePart type="family">Ren</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yan</namePart><namePart type="family">Liu</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xiaodong</namePart><namePart type="family">Li</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Siyu</namePart><namePart type="family">Bai</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yanwei</namePart><namePart type="family">Zhong</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lin</namePart><namePart type="family">Wang</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Panyong</namePart><namePart type="family">Mao</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Huifen</namePart><namePart type="family">Wang</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shaojie</namePart><namePart type="family">Xin</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Vincent</namePart><namePart type="given">Wai-Sun</namePart><namePart type="family">Wong</namePart><affiliation>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Henry</namePart><namePart type="given">Lik-Yuen</namePart><namePart type="family">Chan</namePart><affiliation>Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Fabien</namePart><namePart type="family">Zoulim</namePart><affiliation>INSERM, U871, 69003, Lyon, France</affiliation><affiliation>Department of Hepatology and Gastroenterology, Hospices Civils de Lyon, Hôtel Dieu Hospital, 69002, Lyon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Dongping</namePart><namePart type="family">Xu</namePart><affiliation>Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China</affiliation><affiliation>Liver Failure Research Center, Beijing 302 Hospital, 100039, Beijing, China</affiliation><affiliation>E-mail: xudongping@yahoo.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Japan</publisher><place><placeTerm type="text">Japan</placeTerm></place><dateCreated encoding="w3cdtf">2010-05-11</dateCreated><dateIssued encoding="w3cdtf">2011-03-01</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Objective: To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features. Patients and methods: Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) patients admitted to or who had visited Beijing 302 Hospital from January 2005 to December 2008 were collected and successfully amplified for the HBV BCP/PC and a 1225-bp-long S/Pol (nt 54–1278) gene regions. Biochemical and serological parameters and HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested PCR. Genotypes/subgenotypes were determined based on complete genomic sequence or on analysis of the 1225-bp-long S/Pol-gene sequence. HBV genotyping was performed by direct PCR sequencing followed by molecular evolutionary analysis of the viral sequences. A P value of <0.05 (two-sided) was considered to be statistically significant. Conclusions: Our findings suggest that CHB patients infected with BCP/PC mutant viruses are more susceptible to severe hepatitis and ACLF than those with the BCP/PC wild-type virus and that ACLF patients with PC mutant viruses have an increased risk of death. As such, the HBV PC mutation is a potential predictive indicator of ACLF outcome.</abstract><note>Original Article—Liver, Pancreas, and Biliary Tract</note><subject lang="en"><genre>Keywords</genre><topic>Hepatitis B virus</topic><topic>Basal core promoter mutation</topic><topic>Precore mutation</topic><topic>Genotype</topic><topic>Acute-on-chronic liver failure</topic></subject><subject><genre>Abbreviations</genre><topic>ACLF : Acute-on-chronic liver failure</topic><topic>ALF : Acute liver failure</topic><topic>ALT : Alanine aminotransferase</topic><topic>BCP : Basal core promoter</topic><topic>CHB-M : Mild chronic hepatitis B</topic><topic>CHB-S : Severe chronic hepatitis B</topic><topic>HBV : Hepatitis B virus</topic><topic>HCC : Hepatocellular carcinoma</topic><topic>LC : Liver cirrhosis</topic><topic>PC : Precore</topic><topic>PTA : Prothrombin activity</topic><topic>TBIL : Total bilirubin</topic><topic>PCR : Polymerase chain reaction</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Gastroenterology</title></titleInfo><titleInfo type="abbreviated"><title>J Gastroenterol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2011-03-31</dateIssued><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><subject><genre>Medicine & Public Health</genre><topic>Colorectal Surgery</topic><topic>Hepatology</topic><topic>Gastroenterology</topic><topic>Internal Medicine</topic><topic>Surgical Oncology</topic><topic>Oncology</topic></subject><identifier type="ISSN">0944-1174</identifier><identifier type="eISSN">1435-5922</identifier><identifier type="JournalID">535</identifier><identifier type="IssueArticleCount">19</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>2011</date><detail type="volume"><number>46</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="pages"><start>391</start><end>400</end></extent></part><recordInfo><recordOrigin>Springer, 2011</recordOrigin></recordInfo></relatedItem><identifier type="istex">E0C7FCC069FEDE1A2C6F6010B6CB8895F3EF8BA0</identifier><identifier type="ark">ark:/67375/VQC-RQ7N7HGC-P</identifier><identifier type="DOI">10.1007/s00535-010-0315-4</identifier><identifier type="ArticleID">315</identifier><identifier type="ArticleID">s00535-010-0315-4</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer, 2010</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer, 2010</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-RQ7N7HGC-P/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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