Rapid and real-time detection technologies for emerging viruses of biomedical importance
Identifieur interne : 000665 ( Istex/Corpus ); précédent : 000664; suivant : 000666Rapid and real-time detection technologies for emerging viruses of biomedical importance
Auteurs : M. M. ParidaSource :
- Journal of Biosciences [ 0250-5991 ] ; 2008-11-01.
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- KwdEn :
Abstract
Abstract: The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.
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DOI: 10.1007/s12038-008-0079-7
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid and real-time detection technologies for emerging viruses of biomedical importance</title><author><name sortKey="Parida, M M" sort="Parida, M M" uniqKey="Parida M" first="M. M." last="Parida">M. M. Parida</name><affiliation><mods:affiliation>Department of Virology, Defence R & D Establishment, 474 002, Gwalior, India</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: paridamm@rediffmail.com</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E82DE72FAD8929C46D84A31FE3C86A677A8AAD0B</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1007/s12038-008-0079-7</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-1TL2SQWC-2/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000665</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000665</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Rapid and real-time detection technologies for emerging viruses of biomedical importance</title><author><name sortKey="Parida, M M" sort="Parida, M M" uniqKey="Parida M" first="M. M." last="Parida">M. M. Parida</name><affiliation><mods:affiliation>Department of Virology, Defence R & D Establishment, 474 002, Gwalior, India</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: paridamm@rediffmail.com</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Biosciences</title><title level="j" type="abbrev">J Biosci</title><idno type="ISSN">0250-5991</idno><idno type="eISSN">0973-7138</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>India</pubPlace><date type="published" when="2008-11-01">2008-11-01</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="617">617</biblScope><biblScope unit="page" to="628">628</biblScope></imprint><idno type="ISSN">0250-5991</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0250-5991</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Emerging viruses</term><term>LAMP</term><term>rapid detection</term><term>real-time PCR</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>M. M. Parida</name><affiliations><json:string>Department of Virology, Defence R & D Establishment, 474 002, Gwalior, India</json:string><json:string>E-mail: paridamm@rediffmail.com</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Emerging viruses</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LAMP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>rapid detection</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>real-time PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>BIP : backward inner primer</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>BLP : backward loop primer</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>FIP : forward inner primer</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>FLP : forward loop primer</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>FRET : fluorescence resonance energy transfer</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HBV : hepatitis B virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HCV : hepatitis C virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LAMP : loop-mediated isothermal amplification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>NSBA : nucleic acid sequence based amplification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCR : polymerase chain reaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PEI : polyethylenimine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RSV : respiratory syncytial virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RT-PCR : reverse transcription polymerase chain reaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SDA : strand displacement amplification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>3SR : self-sustained sequence replication</value></json:item></subject><articleId><json:string>79</json:string><json:string>s12038-008-0079-7</json:string></articleId><arkIstex>ark:/67375/VQC-1TL2SQWC-2</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</abstract><qualityIndicators><score>9.544</score><pdfWordCount>5918</pdfWordCount><pdfCharCount>36740</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>212</abstractWordCount><abstractCharCount>1516</abstractCharCount><keywordCount>19</keywordCount></qualityIndicators><title>Rapid and real-time detection technologies for emerging viruses of biomedical importance</title><genre><json:string>research-article</json:string></genre><host><title>Journal of Biosciences</title><language><json:string>unknown</json:string></language><publicationDate>2008</publicationDate><copyrightDate>2008</copyrightDate><issn><json:string>0250-5991</json:string></issn><eissn><json:string>0973-7138</json:string></eissn><journalId><json:string>12038</json:string></journalId><volume>33</volume><issue>4</issue><pages><first>617</first><last>628</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Cell Biology</value></json:item><json:item><value>Microbiology</value></json:item><json:item><value>Plant Sciences</value></json:item><json:item><value>Zoology</value></json:item><json:item><value>Biomedicine general</value></json:item><json:item><value>Life Sciences, general</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-1TL2SQWC-2</json:string></ark><publicationDate>2008</publicationDate><copyrightDate>2008</copyrightDate><doi><json:string>10.1007/s12038-008-0079-7</json:string></doi><id>E82DE72FAD8929C46D84A31FE3C86A677A8AAD0B</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-1TL2SQWC-2/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-1TL2SQWC-2/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/VQC-1TL2SQWC-2/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Rapid and real-time detection technologies for emerging viruses of biomedical importance</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Springer-Verlag</publisher><pubPlace>India</pubPlace><availability><licence><p>Indian Academy of Sciences, 2008</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>2008</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Rapid and real-time detection technologies for emerging viruses of biomedical importance</title><author xml:id="author-0000" corresp="yes"><persName><forename type="first">M.</forename><surname>Parida</surname></persName><email>paridamm@rediffmail.com</email><affiliation>Department of Virology, Defence R & D Establishment, 474 002, Gwalior, India</affiliation></author><idno type="istex">E82DE72FAD8929C46D84A31FE3C86A677A8AAD0B</idno><idno type="ark">ark:/67375/VQC-1TL2SQWC-2</idno><idno type="DOI">10.1007/s12038-008-0079-7</idno><idno type="article-id">79</idno><idno type="article-id">s12038-008-0079-7</idno></analytic><monogr><title level="j">Journal of Biosciences</title><title level="j" type="abbrev">J Biosci</title><idno type="pISSN">0250-5991</idno><idno type="eISSN">0973-7138</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">19</idno><idno type="volume-issue-count">6</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>India</pubPlace><date type="published" when="2008-11-01"></date><biblScope unit="volume">33</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="617">617</biblScope><biblScope unit="page" to="628">628</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2008</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Emerging viruses</term></item><item><term>LAMP</term></item><item><term>rapid detection</term></item><item><term>real-time PCR</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations used</head><item><term>BIP</term><term>backward inner primer</term></item><item><term>BLP</term><term>backward loop primer</term></item><item><term>FIP</term><term>forward inner primer</term></item><item><term>FLP</term><term>forward loop primer</term></item><item><term>FRET</term><term>fluorescence resonance energy transfer</term></item><item><term>HBV</term><term>hepatitis B virus</term></item><item><term>HCV</term><term>hepatitis C virus</term></item><item><term>LAMP</term><term>loop-mediated isothermal amplification</term></item><item><term>NSBA</term><term>nucleic acid sequence based amplification</term></item><item><term>PCR</term><term>polymerase chain reaction</term></item><item><term>PEI</term><term>polyethylenimine</term></item><item><term>RSV</term><term>respiratory syncytial virus</term></item><item><term>RT-PCR</term><term>reverse transcription polymerase chain reaction</term></item><item><term>SDA</term><term>strand displacement amplification</term></item><item><term>3SR</term><term>self-sustained sequence replication</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Cell Biology</term></item><item><term>Microbiology</term></item><item><term>Plant Sciences</term></item><item><term>Zoology</term></item><item><term>Biomedicine general</term></item><item><term>Life Sciences, 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importance</ArticleTitle><ArticleFirstPage>617</ArticleFirstPage><ArticleLastPage>628</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2008</Year><Month>12</Month><Day>20</Day></RegistrationDate><OnlineDate><Year>2008</Year><Month>12</Month><Day>24</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Indian Academy of Sciences</CopyrightHolderName><CopyrightYear>2008</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><GivenName>M.</GivenName><FamilyName>Parida</FamilyName></AuthorName><Contact><Email>paridamm@rediffmail.com</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Department of Virology</OrgDivision><OrgName>Defence R & D Establishment</OrgName><OrgAddress><City>Gwalior</City><Postcode>474 002</Postcode><Country Code="IN">India</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>Emerging viruses</Keyword><Keyword>LAMP</Keyword><Keyword>rapid detection</Keyword><Keyword>real-time PCR</Keyword></KeywordGroup><AbbreviationGroup><Heading>Abbreviations used</Heading><DefinitionList><DefinitionListEntry><Term>BIP</Term><Description><Para>backward inner primer</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>BLP</Term><Description><Para>backward loop primer</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>FIP</Term><Description><Para>forward inner primer</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>FLP</Term><Description><Para>forward loop primer</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>FRET</Term><Description><Para>fluorescence resonance energy transfer</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>HBV</Term><Description><Para>hepatitis B virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>HCV</Term><Description><Para>hepatitis C virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>LAMP</Term><Description><Para>loop-mediated isothermal amplification</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>NSBA</Term><Description><Para>nucleic acid sequence based amplification</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PCR</Term><Description><Para>polymerase chain reaction</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PEI</Term><Description><Para>polyethylenimine</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>RSV</Term><Description><Para>respiratory syncytial virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>RT-PCR</Term><Description><Para>reverse transcription polymerase chain reaction</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>SDA</Term><Description><Para>strand displacement amplification</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>3SR</Term><Description><Para>self-sustained sequence replication</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Rapid and real-time detection technologies for emerging viruses of biomedical importance</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Rapid and real-time detection technologies for emerging viruses of biomedical importance</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">M.</namePart><namePart type="given">M.</namePart><namePart type="family">Parida</namePart><affiliation>Department of Virology, Defence R & D Establishment, 474 002, Gwalior, India</affiliation><affiliation>E-mail: paridamm@rediffmail.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">India</placeTerm></place><dateIssued encoding="w3cdtf">2008-11-01</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</abstract><subject lang="en"><genre>Keywords</genre><topic>Emerging viruses</topic><topic>LAMP</topic><topic>rapid detection</topic><topic>real-time PCR</topic></subject><subject><genre>Abbreviations used</genre><topic>BIP : backward inner primer</topic><topic>BLP : backward loop primer</topic><topic>FIP : forward inner primer</topic><topic>FLP : forward loop primer</topic><topic>FRET : fluorescence resonance energy transfer</topic><topic>HBV : hepatitis B virus</topic><topic>HCV : hepatitis C virus</topic><topic>LAMP : loop-mediated isothermal amplification</topic><topic>NSBA : nucleic acid sequence based amplification</topic><topic>PCR : polymerase chain reaction</topic><topic>PEI : polyethylenimine</topic><topic>RSV : respiratory syncytial virus</topic><topic>RT-PCR : reverse transcription polymerase chain reaction</topic><topic>SDA : strand displacement amplification</topic><topic>3SR : self-sustained sequence replication</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Biosciences</title></titleInfo><titleInfo type="abbreviated"><title>J Biosci</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2008-12-24</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Cell Biology</topic><topic>Microbiology</topic><topic>Plant Sciences</topic><topic>Zoology</topic><topic>Biomedicine general</topic><topic>Life Sciences, general</topic></subject><identifier type="ISSN">0250-5991</identifier><identifier type="eISSN">0973-7138</identifier><identifier type="JournalID">12038</identifier><identifier type="IssueArticleCount">19</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>2008</date><detail type="volume"><number>33</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="pages"><start>617</start><end>628</end></extent></part><recordInfo><recordOrigin>Indian Academy of Sciences, 2008</recordOrigin></recordInfo></relatedItem><identifier type="istex">E82DE72FAD8929C46D84A31FE3C86A677A8AAD0B</identifier><identifier type="ark">ark:/67375/VQC-1TL2SQWC-2</identifier><identifier type="DOI">10.1007/s12038-008-0079-7</identifier><identifier type="ArticleID">79</identifier><identifier type="ArticleID">s12038-008-0079-7</identifier><accessCondition type="use and reproduction" contentType="copyright">Indian Academy of Sciences, 2008</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Indian Academy of Sciences, 2008</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-1TL2SQWC-2/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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