Determination of SARS‐coronavirus by a microfluidic chip system
Identifieur interne : 000219 ( Istex/Corpus ); précédent : 000218; suivant : 000220Determination of SARS‐coronavirus by a microfluidic chip system
Auteurs : Xiaomian Zhou ; Dayu Liu ; Runtao Zhong ; Zhongpeng Dai ; Dapeng Wu ; Hui Wang ; Yuguang Du ; Zhinan Xia ; Liping Zhang ; Xiaodai Mei ; Bingcheng LinSource :
- ELECTROPHORESIS [ 0173-0835 ] ; 2004-09.
English descriptors
- Teeft :
- Alternative methods, Amplification, Assay, Cdna, Chem, Chemical physics, Chinese academy, Clinical sars samples, Clinical specimens, Dalian institute, Detection limit, Detection system, Duplex, Early stage, Electrophoresis, Electrophoretic analysis, Electrophoretic sizing, Gmbh, High sensitivity, Intrareservoir temperature, Kgaa, Medical university, Microchip, Microfluidic, Microfluidic chip, Microfluidic chip analyzer, Microfluidic chip system, Migration time, Molecular test, Nasopharyngeal swabs, Negative control, Nucleic acid stain, Parainfluenza virus, Peltier, Peltier surface temperature, Photomultiplier tube, Positive control, Positive rate, Primer, Reaction mixture, Restriction fragments, Rnaeasy mini, Same chip, Same time, Sample reservoir, Sample waste, Sample waste reservoirs, Sars, Sars genome sequence, Sars patients, Takara biotechnology, Thermal cycler, Verlag, Verlag gmbh, Virus isolation, Waste reservoirs, Weinheim, Weinheim figure.
Abstract
We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS‐CoV). The system includes a laser‐induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription‐polymerase chain reaction (RT‐PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS‐CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT‐PCR on SARS‐CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home‐made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS‐CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT‐PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.
Url:
DOI: 10.1002/elps.200305966
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ISTEX:C2F2F43A895E4AD967AB7FE47371E395E3AF5D07Le document en format XML
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China</mods:affiliation></affiliation></author><author><name sortKey="Liu, Dayu" sort="Liu, Dayu" uniqKey="Liu D" first="Dayu" last="Liu">Dayu Liu</name><affiliation><mods:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Zhong, Runtao" sort="Zhong, Runtao" uniqKey="Zhong R" first="Runtao" last="Zhong">Runtao Zhong</name><affiliation><mods:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Dai, Zhongpeng" sort="Dai, Zhongpeng" uniqKey="Dai Z" first="Zhongpeng" last="Dai">Zhongpeng Dai</name><affiliation><mods:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Wu, Dapeng" sort="Wu, Dapeng" uniqKey="Wu D" first="Dapeng" last="Wu">Dapeng Wu</name><affiliation><mods:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name><affiliation><mods:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Du, Yuguang" sort="Du, Yuguang" uniqKey="Du Y" first="Yuguang" last="Du">Yuguang Du</name><affiliation><mods:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Xia, Zhinan" sort="Xia, Zhinan" uniqKey="Xia Z" first="Zhinan" last="Xia">Zhinan Xia</name><affiliation><mods:affiliation>Dana Fabre Cancer Research Institute, Harvard Medical School, Longwood, Boston, MA, USA</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Liping" sort="Zhang, Liping" uniqKey="Zhang L" first="Liping" last="Zhang">Liping Zhang</name><affiliation><mods:affiliation>Genomic Department, Wyeth Pharmaceuticals, Inc., Cambridge, MA, USA</mods:affiliation></affiliation></author><author><name sortKey="Mei, Xiaodai" sort="Mei, Xiaodai" uniqKey="Mei X" first="Xiaodai" last="Mei">Xiaodai Mei</name><affiliation><mods:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</mods:affiliation></affiliation></author><author><name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name><affiliation><mods:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: bclin@dicp.ac.cn</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China +86‐0411‐84379065===</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">ELECTROPHORESIS</title><title level="j" type="alt">ELECTROPHORESIS</title><idno type="ISSN">0173-0835</idno><idno type="eISSN">1522-2683</idno><imprint><biblScope unit="vol">25</biblScope><biblScope unit="issue">17</biblScope><biblScope unit="page" from="3032">3032</biblScope><biblScope unit="page" to="3039">3039</biblScope><biblScope unit="page-count">8</biblScope><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2004-09">2004-09</date></imprint><idno type="ISSN">0173-0835</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0173-0835</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Alternative methods</term><term>Amplification</term><term>Assay</term><term>Cdna</term><term>Chem</term><term>Chemical physics</term><term>Chinese academy</term><term>Clinical sars samples</term><term>Clinical specimens</term><term>Dalian institute</term><term>Detection limit</term><term>Detection system</term><term>Duplex</term><term>Early stage</term><term>Electrophoresis</term><term>Electrophoretic analysis</term><term>Electrophoretic sizing</term><term>Gmbh</term><term>High sensitivity</term><term>Intrareservoir temperature</term><term>Kgaa</term><term>Medical university</term><term>Microchip</term><term>Microfluidic</term><term>Microfluidic chip</term><term>Microfluidic chip analyzer</term><term>Microfluidic chip system</term><term>Migration time</term><term>Molecular test</term><term>Nasopharyngeal swabs</term><term>Negative control</term><term>Nucleic acid stain</term><term>Parainfluenza virus</term><term>Peltier</term><term>Peltier surface temperature</term><term>Photomultiplier tube</term><term>Positive control</term><term>Positive rate</term><term>Primer</term><term>Reaction mixture</term><term>Restriction fragments</term><term>Rnaeasy mini</term><term>Same chip</term><term>Same time</term><term>Sample reservoir</term><term>Sample waste</term><term>Sample waste reservoirs</term><term>Sars</term><term>Sars genome sequence</term><term>Sars patients</term><term>Takara biotechnology</term><term>Thermal cycler</term><term>Verlag</term><term>Verlag gmbh</term><term>Virus isolation</term><term>Waste reservoirs</term><term>Weinheim</term><term>Weinheim figure</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS‐CoV). The system includes a laser‐induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription‐polymerase chain reaction (RT‐PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS‐CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT‐PCR on SARS‐CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home‐made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS‐CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT‐PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>sars</json:string><json:string>microfluidic</json:string><json:string>primer</json:string><json:string>chem</json:string><json:string>microfluidic chip system</json:string><json:string>peltier</json:string><json:string>weinheim</json:string><json:string>kgaa</json:string><json:string>cdna</json:string><json:string>verlag gmbh</json:string><json:string>gmbh</json:string><json:string>verlag</json:string><json:string>microchip</json:string><json:string>sars patients</json:string><json:string>assay</json:string><json:string>detection system</json:string><json:string>sample reservoir</json:string><json:string>nasopharyngeal swabs</json:string><json:string>chinese academy</json:string><json:string>negative control</json:string><json:string>duplex</json:string><json:string>parainfluenza virus</json:string><json:string>dalian institute</json:string><json:string>thermal cycler</json:string><json:string>reaction mixture</json:string><json:string>positive control</json:string><json:string>detection limit</json:string><json:string>same time</json:string><json:string>high sensitivity</json:string><json:string>chemical physics</json:string><json:string>amplification</json:string><json:string>sample waste</json:string><json:string>virus isolation</json:string><json:string>electrophoretic analysis</json:string><json:string>clinical specimens</json:string><json:string>restriction fragments</json:string><json:string>takara biotechnology</json:string><json:string>nucleic acid stain</json:string><json:string>medical university</json:string><json:string>molecular test</json:string><json:string>rnaeasy mini</json:string><json:string>same chip</json:string><json:string>early stage</json:string><json:string>weinheim figure</json:string><json:string>alternative methods</json:string><json:string>electrophoretic sizing</json:string><json:string>waste reservoirs</json:string><json:string>sample waste reservoirs</json:string><json:string>microfluidic chip analyzer</json:string><json:string>peltier surface temperature</json:string><json:string>microfluidic chip</json:string><json:string>photomultiplier tube</json:string><json:string>clinical sars samples</json:string><json:string>sars genome sequence</json:string><json:string>intrareservoir temperature</json:string><json:string>migration time</json:string><json:string>positive rate</json:string><json:string>electrophoresis</json:string></teeft></keywords><author><json:item><name>Xiaomian Zhou</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string></affiliations></json:item><json:item><name>Dayu Liu</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string></affiliations></json:item><json:item><name>Runtao Zhong</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string></affiliations></json:item><json:item><name>Zhongpeng Dai</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string></affiliations></json:item><json:item><name>Dapeng Wu</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string></affiliations></json:item><json:item><name>Hui Wang</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string></affiliations></json:item><json:item><name>Yuguang Du</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string></affiliations></json:item><json:item><name>Zhinan Xia</name><affiliations><json:string>Dana Fabre Cancer Research Institute, Harvard Medical School, Longwood, Boston, MA, USA</json:string></affiliations></json:item><json:item><name>Liping Zhang</name><affiliations><json:string>Genomic Department, Wyeth Pharmaceuticals, Inc., Cambridge, MA, USA</json:string></affiliations></json:item><json:item><name>Xiaodai Mei</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string></affiliations></json:item><json:item><name>Bingcheng Lin</name><affiliations><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</json:string><json:string>E-mail: bclin@dicp.ac.cn</json:string><json:string>Correspondence address: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China +86‐0411‐84379065===</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Microfluidic chip system</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Miniaturization</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Multiplex reverse transcription‐polymerase chain reaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>On‐line chip polymerase chain reaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Severe acute respiratory syndrome</value></json:item></subject><articleId><json:string>ELPS200305966</json:string></articleId><arkIstex>ark:/67375/WNG-QSFCGF3K-0</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS‐CoV). The system includes a laser‐induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription‐polymerase chain reaction (RT‐PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS‐CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT‐PCR on SARS‐CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home‐made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS‐CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT‐PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.</abstract><qualityIndicators><score>8.279</score><pdfWordCount>4071</pdfWordCount><pdfCharCount>24746</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>595 x 842 pts (A4)</pdfPageSize><pdfWordsPerPage>509</pdfWordsPerPage><pdfText>true</pdfText><refBibsNative>true</refBibsNative><abstractWordCount>184</abstractWordCount><abstractCharCount>1295</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>Determination of SARS‐coronavirus by a microfluidic chip system</title><pmid><json:string>15349945</json:string></pmid><genre><json:string>article</json:string></genre><host><title>ELECTROPHORESIS</title><language><json:string>unknown</json:string></language><doi><json:string>10.1002/(ISSN)1522-2683</json:string></doi><issn><json:string>0173-0835</json:string></issn><eissn><json:string>1522-2683</json:string></eissn><publisherId><json:string>ELPS</json:string></publisherId><volume>25</volume><issue>17</issue><pages><first>3032</first><last>3039</last><total>8</total></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Research Article</value></json:item></subject></host><namedEntities><unitex><date><json:string>2003</json:string><json:string>2004</json:string><json:string>3-4-10</json:string></date><geogName></geogName><orgName><json:string>Dana Fabre Cancer Research Institute, Harvard Medical School, Longwood, Boston, MA, USA</json:string><json:string>Genomic Department, Wyeth Pharmaceuticals, Inc</json:string><json:string>Sigma Chemical</json:string><json:string>Qiagen</json:string><json:string>Military Medical University</json:string><json:string>TaKaRa Biotechnology, Dalian, China</json:string><json:string>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian</json:string><json:string>Biotech</json:string><json:string>Mektec Seiwa Corporation, Beijing, China</json:string><json:string>BNI</json:string><json:string>TaKaRa Biotechnology</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>To</json:string><json:string>Bingcheng Lin</json:string></persName><placeName><json:string>Tianjin City</json:string><json:string>Germany</json:string><json:string>Pittsburgh</json:string><json:string>Hamburg</json:string><json:string>China</json:string><json:string>USA</json:string><json:string>Japan</json:string><json:string>MA</json:string><json:string>Cambridge</json:string><json:string>PA</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>[4]</json:string><json:string>[18]</json:string><json:string>[6]</json:string><json:string>Khandurina et al. [15]</json:string><json:string>[19, 20]</json:string><json:string>[1]</json:string><json:string>[16]</json:string><json:string>Poon et al. [5]</json:string><json:string>[15]</json:string><json:string>[11, 12]</json:string><json:string>[14]</json:string><json:string>X. Zhou et al.</json:string><json:string>[2]</json:string><json:string>[13]</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-QSFCGF3K-0</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - chemistry, analytical</json:string><json:string>2 - biochemical research methods</json:string></wos><scienceMetrix><json:string>1 - natural sciences</json:string><json:string>2 - chemistry</json:string><json:string>3 - analytical chemistry</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Clinical Biochemistry</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Biochemistry</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - invertebres</json:string></inist></categories><publicationDate>2004</publicationDate><copyrightDate>2004</copyrightDate><doi><json:string>10.1002/elps.200305966</json:string></doi><id>C2F2F43A895E4AD967AB7FE47371E395E3AF5D07</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-QSFCGF3K-0/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-QSFCGF3K-0/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/WNG-QSFCGF3K-0/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Determination of SARS‐coronavirus by a microfluidic chip system</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><availability><licence>Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. 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China</addrLine><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author xml:id="author-0001"><persName><forename type="first">Dayu</forename><surname>Liu</surname></persName><affiliation><orgName type="institution">Dalian Institute of Chemical Physics</orgName><orgName type="institution">Chinese Academy of Sciences</orgName><address><addrLine>Dalian</addrLine><addrLine>P. R. China</addrLine><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">Runtao</forename><surname>Zhong</surname></persName><affiliation><orgName type="institution">Dalian Institute of Chemical Physics</orgName><orgName type="institution">Chinese Academy of Sciences</orgName><address><addrLine>Dalian</addrLine><addrLine>P. R. 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China</addrLine><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author xml:id="author-0007"><persName><forename type="first">Zhinan</forename><surname>Xia</surname></persName><affiliation><orgName type="institution">Dana Fabre Cancer Research Institute</orgName><orgName type="department">Harvard Medical School</orgName><address><addrLine>Longwood</addrLine><addrLine>Boston, MA, USA</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation></author><author xml:id="author-0008"><persName><forename type="first">Liping</forename><surname>Zhang</surname></persName><affiliation><orgName type="division">Genomic Department</orgName><address><addrLine>Wyeth Pharmaceuticals</addrLine><addrLine>Inc., Cambridge, MA, USA</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation></author><author xml:id="author-0009"><persName><forename type="first">Xiaodai</forename><surname>Mei</surname></persName><affiliation><orgName type="institution">Dalian Institute of Chemical Physics</orgName><orgName type="institution">Chinese Academy of Sciences</orgName><address><addrLine>Dalian</addrLine><addrLine>P. R. China</addrLine><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author xml:id="author-0010" role="corresp"><persName><forename type="first">Bingcheng</forename><surname>Lin</surname></persName><email>bclin@dicp.ac.cn</email><affiliation><orgName type="institution">Dalian Institute of Chemical Physics</orgName><orgName type="institution">Chinese Academy of Sciences</orgName><address><addrLine>Dalian</addrLine><addrLine>P. R. 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China</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="US" type="organization"><unparsedAffiliation>Dana Fabre Cancer Research Institute, Harvard Medical School, Longwood, Boston, MA, USA</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="US" type="organization"><unparsedAffiliation>Genomic Department, Wyeth Pharmaceuticals, Inc., Cambridge, MA, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">Microfluidic chip system</keyword><keyword xml:id="kwd2">Miniaturization</keyword><keyword xml:id="kwd3">Multiplex reverse transcription‐polymerase chain reaction</keyword><keyword xml:id="kwd4">On‐line chip polymerase chain reaction</keyword><keyword xml:id="kwd5">Severe acute respiratory syndrome</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS‐CoV). The system includes a laser‐induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription‐polymerase chain reaction (RT‐PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS‐CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT‐PCR on SARS‐CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home‐made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS‐CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT‐PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Determination of SARS‐coronavirus by a microfluidic chip system</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Determination of SARS‐coronavirus by a microfluidic chip system</title></titleInfo><name type="personal"><namePart type="given">Xiaomian</namePart><namePart type="family">Zhou</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dayu</namePart><namePart type="family">Liu</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Runtao</namePart><namePart type="family">Zhong</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhongpeng</namePart><namePart type="family">Dai</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dapeng</namePart><namePart type="family">Wu</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hui</namePart><namePart type="family">Wang</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yuguang</namePart><namePart type="family">Du</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhinan</namePart><namePart type="family">Xia</namePart><affiliation>Dana Fabre Cancer Research Institute, Harvard Medical School, Longwood, Boston, MA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Liping</namePart><namePart type="family">Zhang</namePart><affiliation>Genomic Department, Wyeth Pharmaceuticals, Inc., Cambridge, MA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xiaodai</namePart><namePart type="family">Mei</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bingcheng</namePart><namePart type="family">Lin</namePart><affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China</affiliation><affiliation>E-mail: bclin@dicp.ac.cn</affiliation><affiliation>Correspondence address: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China +86‐0411‐84379065===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">2004-09</dateIssued><dateCaptured encoding="w3cdtf">2003-12-30</dateCaptured><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">6</extent><extent unit="tables">0</extent><extent unit="references">20</extent></physicalDescription><abstract lang="en">We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS‐CoV). The system includes a laser‐induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription‐polymerase chain reaction (RT‐PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS‐CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT‐PCR on SARS‐CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home‐made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS‐CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT‐PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.</abstract><subject lang="en"><genre>keywords</genre><topic>Microfluidic chip system</topic><topic>Miniaturization</topic><topic>Multiplex reverse transcription‐polymerase chain reaction</topic><topic>On‐line chip polymerase chain reaction</topic><topic>Severe acute respiratory syndrome</topic></subject><relatedItem type="host"><titleInfo><title>ELECTROPHORESIS</title></titleInfo><titleInfo type="abbreviated"><title>ELECTROPHORESIS</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0173-0835</identifier><identifier type="eISSN">1522-2683</identifier><identifier type="DOI">10.1002/(ISSN)1522-2683</identifier><identifier type="PublisherID">ELPS</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>25</number></detail><detail type="issue"><caption>no.</caption><number>17</number></detail><extent unit="pages"><start>3032</start><end>3039</end><total>8</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>WHO Summary table of SARS cases by country</title></titleInfo><genre>other</genre></relatedItem><relatedItem type="references" displayLabel="cit2"><titleInfo><title>SARS: Laboratory diagnosis tests</title></titleInfo><genre>other</genre></relatedItem><relatedItem type="references" displayLabel="cit3"><titleInfo><title>Clin. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">McIntosh, K., Clin. Chem. 2003, 49, 845–846.</note><name type="personal"><namePart type="given">K.</namePart><namePart type="family">McIntosh</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>49</number></detail><extent unit="pages"><start>845</start><end>846</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit4"><titleInfo><title>Hoard, J. C.,Schultz, T. F., in:Collins, M.,Clifton, N. J. (Eds.),Methods in Molecular Biology,Humana Press,Totowa, NJ1991,Vol. 8.</title></titleInfo><note type="citation/reference">Hoard, J. C., Schultz, T. F., in: Collins, M., Clifton, N. J. (Eds.), Methods in Molecular Biology, Humana Press, Totowa, NJ 1991, Vol. 8.</note><name type="personal"><namePart type="given">J. C.</namePart><namePart type="family">Hoard</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T. F.</namePart><namePart type="family">Schultz</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Collins</namePart><role><roleTerm type="text">editor</roleTerm></role></name><name type="personal"><namePart type="given">N. J.</namePart><namePart type="family">Clifton</namePart><role><roleTerm type="text">editor</roleTerm></role></name><genre>book</genre><originInfo><publisher>Humana Press</publisher><place><placeTerm type="text">Totowa, NJ</placeTerm></place></originInfo><part><date>1991</date></part></relatedItem><relatedItem type="references" displayLabel="cit5"><titleInfo><title>Clin. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Poon, L. L. M., Wong, O. K., Luk, W., Yuen, K. Y., Peiris, J. S. M., Guan, Y., Clin. Chem. 2003, 49, 845–846.</note><name type="personal"><namePart type="given">L. L. M.</namePart><namePart type="family">Poon</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O. K.</namePart><namePart type="family">Wong</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Luk</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. Y.</namePart><namePart type="family">Yuen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. S. M.</namePart><namePart type="family">Peiris</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Guan</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>49</number></detail><extent unit="pages"><start>845</start><end>846</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit6"><titleInfo><title>Chin. Med. J.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Wu, X. W., Cheng, G., Di, B., Yin, A. H., He, Y. S., Wang, M., Zhou, X.Y., He, L. J., Luo, K., Du, L., Chin. Med. J. 2003, 116, 988–990.</note><name type="personal"><namePart type="given">X. W.</namePart><namePart type="family">Wu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Cheng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Di</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. H.</namePart><namePart type="family">Yin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y. S.</namePart><namePart type="family">He</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X.Y.</namePart><namePart type="family">Zhou</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. J.</namePart><namePart type="family">He</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Luo</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Du</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>116</number></detail><extent unit="pages"><start>988</start><end>990</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit7"><titleInfo><title>Chem. Eng. News</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Freemantal, M., Chem. Eng. News 1999, 22, 27–36.</note><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Freemantal</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>22</number></detail><extent unit="pages"><start>27</start><end>36</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit8"><titleInfo><title>Anal. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Northrup, M. A., Benett, B., Hadley, D., Long, G., Landre, P., Lehew, S., Richards, J., Stratton, P., Anal. Chem. 1998, 70, 918–992.</note><name type="personal"><namePart type="given">M. A.</namePart><namePart type="family">Northrup</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Benett</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Hadley</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Long</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Landre</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Lehew</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Richards</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Stratton</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>70</number></detail><extent unit="pages"><start>918</start><end>992</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit9"><titleInfo><title>Clin. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Belgrader, P., Benett, W., Hadley, D., Long, G., Mariella, R., Milanovich, F., Nelson, W., Richards, J., Stratton, P., Clin. Chem. 1998, 44, 2191–2194.</note><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Belgrader</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Benett</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Hadley</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Long</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Mariella</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Milanovich</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Nelson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Richards</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Stratton</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>44</number></detail><extent unit="pages"><start>2191</start><end>2194</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit10"><titleInfo><title>Science</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Belgrader, P., Benett, W., Hadley, D., Richards, J., Stratton, P., Mariella, R., Milanovich, F., Science 1999, 284, 449–450.</note><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Belgrader</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Benett</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Hadley</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Richards</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Stratton</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Mariella</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Milanovich</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>284</number></detail><extent unit="pages"><start>449</start><end>450</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit11"><titleInfo><title>Anal. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Ibrahim, M., Lofts, R., Jahrling, P., Henchal, E., Weedn, V., Northrup, M. A., Belgrader, P., Anal. Chem. 1998, 70, 2013–2017.</note><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Ibrahim</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Lofts</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Jahrling</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Henchal</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Weedn</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. A.</namePart><namePart type="family">Northrup</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Belgrader</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>70</number></detail><extent unit="pages"><start>2013</start><end>2017</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit12"><titleInfo><title>Forensic Sci.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Belgrader, P., Smith, J., Weedn, V., Northrup, M. A. J., Forensic Sci. 1998, 43, 315–319.</note><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Belgrader</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Smith</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Weedn</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. A. J.</namePart><namePart type="family">Northrup</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>43</number></detail><extent unit="pages"><start>315</start><end>319</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit13"><titleInfo><title>Anal. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Effenhauser, C. S., Bruin, G. J. M., Paulus, A., Ehrat, M., Anal. Chem. 1997, 69, 3451–3457.</note><name type="personal"><namePart type="given">C. S.</namePart><namePart type="family">Effenhauser</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. J. M.</namePart><namePart type="family">Bruin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Paulus</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Ehrat</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>69</number></detail><extent unit="pages"><start>3451</start><end>3457</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit14"><titleInfo><title>Lab Chip.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Yang, J., Liu, Y. J., Rauch, C. B., Stevens, R. H., Liu, R. L., Lenigk, R., Grodzinski, P., Lab Chip. 2002, 2, 179–187.</note><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y. J.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. B.</namePart><namePart type="family">Rauch</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. H.</namePart><namePart type="family">Stevens</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. L.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Lenigk</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Grodzinski</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>2</number></detail><extent unit="pages"><start>179</start><end>187</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit15"><titleInfo><title>Anal. Chem.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Khandurina, J., Mcknight, T. E., Jacobson, S. C., Waters, L. C., Foote, R. S., Ramsey, J. M., Anal. Chem. 2000, 72, 2995–3000.</note><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Khandurina</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T. E.</namePart><namePart type="family">Mcknight</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. C.</namePart><namePart type="family">Jacobson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. C.</namePart><namePart type="family">Waters</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. S.</namePart><namePart type="family">Foote</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. M.</namePart><namePart type="family">Ramsey</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2000</date><detail type="volume"><caption>vol.</caption><number>72</number></detail><extent unit="pages"><start>2995</start><end>3000</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit16"><titleInfo><title>Proc. Natl. Acad. Sci. USA</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Woolley, A. T., Mathies, R. A., Proc. Natl. Acad. Sci. USA 1994, 91, 11348.</note><name type="personal"><namePart type="given">A. T.</namePart><namePart type="family">Woolley</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. A.</namePart><namePart type="family">Mathies</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>91</number></detail><extent unit="pages"><start>11348</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit17"><titleInfo><title>Science</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Kopp, M. U., deMello, A. J., Manz, A., Science 1998, 280, 1046–1047.</note><name type="personal"><namePart type="given">M. U.</namePart><namePart type="family">Kopp</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. J.</namePart><namePart type="family">deMello</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Manz</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>280</number></detail><extent unit="pages"><start>1046</start><end>1047</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit18"><titleInfo><title>Chem. J. Chin. Univ.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Zhou, X. M., Dai, Z. P., Luo, Y., Liu, X., Wang, H., Lin, B. C., Chem. J. Chin. Univ. 2004, 3, 336–340.</note><name type="personal"><namePart type="given">X. M.</namePart><namePart type="family">Zhou</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z. P.</namePart><namePart type="family">Dai</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Luo</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B. C.</namePart><namePart type="family">Lin</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><extent unit="pages"><start>336</start><end>340</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit19"><titleInfo><title>Clin. Diagn. Virol.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Valassina, M., Cuppone, A. M., Cusi, M. G., Valensin, P. E., Clin. Diagn. Virol. 1997, 8, 227–232.</note><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Valassina</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. M.</namePart><namePart type="family">Cuppone</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. G.</namePart><namePart type="family">Cusi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. E.</namePart><namePart type="family">Valensin</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>8</number></detail><extent unit="pages"><start>227</start><end>232</end></extent></part></relatedItem><relatedItem type="references" displayLabel="cit20"><titleInfo><title>J. Med. Virol.</title></titleInfo><genre>journal-article</genre><note type="citation/reference">Cassinotti, P., Mietz, H., Siegl, G., J. Med. Virol. 1996, 50, 75–81.</note><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Cassinotti</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Mietz</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Siegl</namePart><role><roleTerm type="text">author</roleTerm></role></name><part><date>1996</date><detail type="volume"><caption>vol.</caption><number>50</number></detail><extent unit="pages"><start>75</start><end>81</end></extent></part></relatedItem><identifier type="istex">C2F2F43A895E4AD967AB7FE47371E395E3AF5D07</identifier><identifier type="ark">ark:/67375/WNG-QSFCGF3K-0</identifier><identifier type="DOI">10.1002/elps.200305966</identifier><identifier type="ArticleID">ELPS200305966</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. 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