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Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses

Identifieur interne : 000117 ( Hal/Curation ); précédent : 000116; suivant : 000118

Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses

Auteurs : Eirini Papagiakoumou [France] ; Vincent De Sars [France] ; Dan Oron [Israël] ; Valentina Emiliani [France]

Source :

RBID : Hal:hal-01963248

English descriptors

Abstract

Multiphoton excitation by temporally focused pulses can be combined with spatial Fourier-transform pulse shaping techniques to enhance spatial control of the excitation volume. Here we propose and demonstrate an optical system for the generation of such spatiotemporally engineered light pulses using a combination of spatial control by a two-dimensional reconfigurable light modulator, with a dispersive optical setup for temporal focusing. We show that although the properties of a holographic beam significantly differ from those of plane-wave illumination used in previous temporal focusing realizations, this leads only to a slightly reduced axial resolution. We show that the system can provide scanning-less, arbitrarily shaped, depth resolved excitation patterns that offer new perspectives for multiphoton photoactivation and optical lithography applications.


Url:
DOI: 10.1364/OE.16.022039

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Hal:hal-01963248

Le document en format XML

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<idno type="halRefHtml">Optics Express, Optical Society of America, 2008, 16 (26), pp.22039-22047. ⟨10.1364/OE.16.022039⟩</idno>
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<idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
<idno type="stamp" n="UNIV-PARIS5" corresp="UNIV-PARIS">Université Paris Descartes (Paris 5)</idno>
<idno type="stamp" n="USPC">Université Sorbonne Paris Cité</idno>
<idno type="stamp" n="UNIV-PARIS">Université de Paris</idno>
<idno type="stamp" n="UP-SCIENCES">Université de Paris - Faculté des Sciences</idno>
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<title xml:lang="en">Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses</title>
<author role="aut">
<persName>
<forename type="first">Eirini</forename>
<surname>Papagiakoumou</surname>
</persName>
<idno type="halauthorid">11233128</idno>
<affiliation ref="#struct-419825"></affiliation>
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<author role="aut">
<persName>
<forename type="first">Vincent</forename>
<surname>De Sars</surname>
</persName>
<idno type="halauthorid">1587885</idno>
<affiliation ref="#struct-419825"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Dan</forename>
<surname>Oron</surname>
</persName>
<idno type="halauthorid">1351413</idno>
<affiliation ref="#struct-5285"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Valentina</forename>
<surname>Emiliani</surname>
</persName>
<email type="md5">9723dbd1d24ced61e5c9150b01bb827e</email>
<email type="domain">parisdescartes.fr</email>
<ptr type="url" target="http://www.biomedicale.univ-paris5.fr/neurophysiologie/Groups/emilianigroup.php"></ptr>
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<idno type="halJournalId" status="VALID">1882</idno>
<idno type="issn">1094-4087</idno>
<title level="j">Optics Express</title>
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<publisher>Optical Society of America</publisher>
<biblScope unit="volume">16</biblScope>
<biblScope unit="issue">26</biblScope>
<biblScope unit="pp">22039-22047</biblScope>
<date type="datePub">2008</date>
<date type="dateEpub">2008-12-19</date>
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<idno type="doi">10.1364/OE.16.022039</idno>
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<language ident="en">English</language>
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<textClass>
<keywords scheme="author">
<term xml:lang="en">Pulse shaping</term>
<term xml:lang="en">Digital holography</term>
<term xml:lang="en">Non-linear microscopy</term>
<term xml:lang="en">Spatial light modulators</term>
</keywords>
<classCode scheme="classification">OCIS: 090.1995, 180.4315, 230.6120, 320.5540</classCode>
<classCode scheme="halDomain" n="spi.opti">Engineering Sciences [physics]/Optics / Photonic</classCode>
<classCode scheme="halDomain" n="nlin">Nonlinear Sciences [physics]</classCode>
<classCode scheme="halTypology" n="ART">Journal articles</classCode>
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<abstract xml:lang="en">
<p>Multiphoton excitation by temporally focused pulses can be combined with spatial Fourier-transform pulse shaping techniques to enhance spatial control of the excitation volume. Here we propose and demonstrate an optical system for the generation of such spatiotemporally engineered light pulses using a combination of spatial control by a two-dimensional reconfigurable light modulator, with a dispersive optical setup for temporal focusing. We show that although the properties of a holographic beam significantly differ from those of plane-wave illumination used in previous temporal focusing realizations, this leads only to a slightly reduced axial resolution. We show that the system can provide scanning-less, arbitrarily shaped, depth resolved excitation patterns that offer new perspectives for multiphoton photoactivation and optical lithography applications.</p>
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