Use of antibody avidity assays for diagnosis of severe acute respiratory syndrome coronavirus infection.
Identifieur interne : 000180 ( Hal/Corpus ); précédent : 000179; suivant : 000181Use of antibody avidity assays for diagnosis of severe acute respiratory syndrome coronavirus infection.
Auteurs : K. H. Chan ; K. Sonnenberg ; M. Niedrig ; S. Y. Lam ; C. M. Pang ; K. M. Chan ; S. K. Ma ; W. H. Seto ; J. S. M. PeirisSource :
- Clinical and Vaccine Immunology [ 1556-6811 ] ; 2007-11.
Abstract
An indirect immunofluorescent assay (Euroimmun AG, Luebeck, Germany) was used to investigate the avidity of immunoglobulin G (IgG), IgM, IgA, and total Ig (IgGAM) antibody responses to severe acute respiratory syndrome coronavirus (SARS CoV) infections. Serial serum samples from eight patients collected during the first, third, and ninth months after the onset of infection were evaluated. It was found that low-avidity IgG antibodies were detected in 15/15 (100%), 1/5 (20%), and 0/8 (0%) serum samples collected during the first, third, and ninth months after the onset of symptoms, respectively. Low-avidity antibodies of IgA and IgM subclasses were detected in 14/14 (100%) and 3/14 (21%) serum samples, respectively, collected in the first month after the onset of infection. However, IgA antibodies remained low in avidity in a proportion of patients even during late convalescence. As a consequence, IgG antibody avidity assays gave better discrimination between acute-phase and late-convalescent-phase serum samples than IgM, IgA, or IgGAM assays. In two of these patients, sequential serum samples were also tested for IgG avidity against human CoV strains OC43 and 229E in parallel. While SARS CoV infections induced an anamnestic IgG antibody response to the 229E and OC43 viruses, these cross-reactive antibodies remained of high avidity from early (the first month) postinfection. The results showed that assays to detect low-avidity antibody may be useful for discriminating early from late antibody responses and also for distinguishing anamnestic cross-reactive antibody responses from primary specific responses. This may be useful in some clinical situations.
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DOI: 10.1128/CVI.00056-07
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<front><div type="abstract" xml:lang="en"> <p>An indirect immunofluorescent assay (Euroimmun AG, Luebeck, Germany) was used to investigate the avidity of immunoglobulin G (IgG), IgM, IgA, and total Ig (IgGAM) antibody responses to severe acute respiratory syndrome coronavirus (SARS CoV) infections. Serial serum samples from eight patients collected during the first, third, and ninth months after the onset of infection were evaluated. It was found that low-avidity IgG antibodies were detected in 15/15 (100%), 1/5 (20%), and 0/8 (0%) serum samples collected during the first, third, and ninth months after the onset of symptoms, respectively. Low-avidity antibodies of IgA and IgM subclasses were detected in 14/14 (100%) and 3/14 (21%) serum samples, respectively, collected in the first month after the onset of infection. However, IgA antibodies remained low in avidity in a proportion of patients even during late convalescence. As a consequence, IgG antibody avidity assays gave better discrimination between acute-phase and late-convalescent-phase serum samples than IgM, IgA, or IgGAM assays. In two of these patients, sequential serum samples were also tested for IgG avidity against human CoV strains OC43 and 229E in parallel. While SARS CoV infections induced an anamnestic IgG antibody response to the 229E and OC43 viruses, these cross-reactive antibodies remained of high avidity from early (the first month) postinfection. The results showed that assays to detect low-avidity antibody may be useful for discriminating early from late antibody responses and also for distinguishing anamnestic cross-reactive antibody responses from primary specific responses. This may be useful in some clinical situations.</p>
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<idno type="halRefHtml">Clinical and Vaccine Immunology, American Society for Microbiology, 2007, 14 (11), pp.1433-6. ⟨10.1128/CVI.00056-07⟩</idno>
<idno type="halRef">Clinical and Vaccine Immunology, American Society for Microbiology, 2007, 14 (11), pp.1433-6. ⟨10.1128/CVI.00056-07⟩</idno>
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<sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Use of antibody avidity assays for diagnosis of severe acute respiratory syndrome coronavirus infection.</title>
<author role="aut"> <persName> <forename type="first">K. H.</forename>
<surname>Chan</surname>
</persName>
<idno type="halauthorid">603628</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">K.</forename>
<surname>Sonnenberg</surname>
</persName>
<idno type="halauthorid">603738</idno>
<affiliation ref="#struct-161276"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">M.</forename>
<surname>Niedrig</surname>
</persName>
<idno type="halauthorid">603739</idno>
<affiliation ref="#struct-478253"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">S. Y.</forename>
<surname>Lam</surname>
</persName>
<idno type="halauthorid">603740</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">C. M.</forename>
<surname>Pang</surname>
</persName>
<idno type="halauthorid">603741</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">K. M.</forename>
<surname>Chan</surname>
</persName>
<idno type="halauthorid">530566</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">S. K.</forename>
<surname>Ma</surname>
</persName>
<idno type="halauthorid">603742</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">W. H.</forename>
<surname>Seto</surname>
</persName>
<idno type="halauthorid">603743</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="crp"> <persName> <forename type="first">J. S. M.</forename>
<surname>Peiris</surname>
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<email type="md5">cc6ecab6abc0bb33758973d51e488f7b</email>
<email type="domain">hkucc.hku.hk</email>
<idno type="halauthorid">603528</idno>
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<monogr> <idno type="halJournalId" status="VALID">11758</idno>
<idno type="issn">1556-6811</idno>
<title level="j">Clinical and Vaccine Immunology</title>
<imprint> <publisher>American Society for Microbiology</publisher>
<biblScope unit="volume">14</biblScope>
<biblScope unit="issue">11</biblScope>
<biblScope unit="pp">1433-6</biblScope>
<date type="datePub">2007-11</date>
<date type="dateEpub">2007-09-19</date>
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<idno type="doi">10.1128/CVI.00056-07</idno>
<idno type="pubmed">17881505</idno>
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<profileDesc> <langUsage> <language ident="en">English</language>
</langUsage>
<textClass> <classCode scheme="mesh">Antibodies, Viral</classCode>
<classCode scheme="mesh">Antibody Affinity</classCode>
<classCode scheme="mesh">SARS Virus</classCode>
<classCode scheme="mesh">Severe Acute Respiratory Syndrome</classCode>
<classCode scheme="mesh">Antibody Specificity</classCode>
<classCode scheme="mesh">Coronavirus 229E, Human</classCode>
<classCode scheme="mesh">Coronavirus OC43, Human</classCode>
<classCode scheme="mesh">Fluorescent Antibody Technique, Indirect</classCode>
<classCode scheme="mesh">Humans</classCode>
<classCode scheme="mesh">Immunoglobulin A</classCode>
<classCode scheme="mesh">Immunoglobulin G</classCode>
<classCode scheme="mesh">Immunoglobulin M</classCode>
<classCode scheme="halDomain" n="sdv.mp.vir">Life Sciences [q-bio]/Microbiology and Parasitology/Virology</classCode>
<classCode scheme="halTypology" n="ART">Journal articles</classCode>
</textClass>
<abstract xml:lang="en"> <p>An indirect immunofluorescent assay (Euroimmun AG, Luebeck, Germany) was used to investigate the avidity of immunoglobulin G (IgG), IgM, IgA, and total Ig (IgGAM) antibody responses to severe acute respiratory syndrome coronavirus (SARS CoV) infections. Serial serum samples from eight patients collected during the first, third, and ninth months after the onset of infection were evaluated. It was found that low-avidity IgG antibodies were detected in 15/15 (100%), 1/5 (20%), and 0/8 (0%) serum samples collected during the first, third, and ninth months after the onset of symptoms, respectively. Low-avidity antibodies of IgA and IgM subclasses were detected in 14/14 (100%) and 3/14 (21%) serum samples, respectively, collected in the first month after the onset of infection. However, IgA antibodies remained low in avidity in a proportion of patients even during late convalescence. As a consequence, IgG antibody avidity assays gave better discrimination between acute-phase and late-convalescent-phase serum samples than IgM, IgA, or IgGAM assays. In two of these patients, sequential serum samples were also tested for IgG avidity against human CoV strains OC43 and 229E in parallel. While SARS CoV infections induced an anamnestic IgG antibody response to the 229E and OC43 viruses, these cross-reactive antibodies remained of high avidity from early (the first month) postinfection. The results showed that assays to detect low-avidity antibody may be useful for discriminating early from late antibody responses and also for distinguishing anamnestic cross-reactive antibody responses from primary specific responses. This may be useful in some clinical situations.</p>
</abstract>
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