Study of p53 expression and post-transcriptional modifications after GSM-900 radiofrequency exposure of human amniotic cells
Identifieur interne : 000150 ( Hal/Corpus ); précédent : 000149; suivant : 000151Study of p53 expression and post-transcriptional modifications after GSM-900 radiofrequency exposure of human amniotic cells
Auteurs : Sylvie Bourthoumieu ; Amandine Magnaudeix ; Faraj Terro ; Philippe Lévêque ; Alice Collin ; Catherine YardinSource :
- Bioelectromagnetics [ 0197-8462 ] ; 2013.
Abstract
The potential effects of radiofrequency (RF) exposure on the genetic material of cells are very important to determine since genome instability of somatic cells may be linked to cancer development. In response to genetic damage, the p53 protein is activated and can induce cell cycle arrest allowing more time for DNA repair or elimination of damaged cells through apoptosis. The objective of this study was to investigate whether the exposure to RF electromagnetic fields, similar to those emitted by mobile phones of the second generation standard, Global System for Mobile Communications (GSM), may induce expression of the p53 protein and its activation by post-translational modifications in cultured human cells. The potential induction of p53 expression and activation by GSM-900 was investigated after in vitro exposure of human amniotic cells for 24 h to average specific absorption rates (SARs) of 0.25, 1, 2, and 4 W/kg in the temperature range of 36.3-39.7 °C. The exposures were carried out using a wire-patch cell (WPC) under strictly controlled conditions of temperature. Expression and activation of p53 by phosphorylation at serine 15 and 37 were studied using Western blot assay immediately after three independent exposures of cell cultures provided from three different donors. Bleomycin-exposed cells were used as a positive control. According to our results, no significant changes in the expression and activation of the p53 protein by phosphorylation at serine 15 and 37 were found following exposure to GSM-900 for 24 h at average SARs up to 4 W/kg in human embryonic cells. Bioelectromagnetics. © 2012 Wiley Periodicals, Inc.
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DOI: 10.1002/bem.21744
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2 avenue Martin Luther King 87042 LIMOGES </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chu-limoges.fr/</ref> </desc> </org></tutelle><tutelle active="#struct-302082" type="direct"><org type="institution" xml:id="struct-302082" status="OLD"> <idno type="RNSR">201220465X</idno> <idno type="IdRef">188832599</idno> <orgName>Génomique, Environnement, Immunité, Santé, Thérapeutique</orgName> <orgName type="acronym">GEIST FR CNRS 3503</orgName> <date type="start">2007-01-01</date> <date type="end">2018-01-01</date> <desc> <address> <addrLine>Université de Limoges - Faculté de Médecine - 2 rue du Dr Marcland 87025 Limoges cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.unilim.fr/recherche/laboratoires/geist/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author></analytic><idno type="DOI">10.1002/bem.21744</idno><series><title level="j">Bioelectromagnetics</title><idno type="ISSN">0197-8462</idno><imprint><date type="datePub">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>The potential effects of radiofrequency (RF) exposure on the genetic material of cells are very important to determine since genome instability of somatic cells may be linked to cancer development. In response to genetic damage, the p53 protein is activated and can induce cell cycle arrest allowing more time for DNA repair or elimination of damaged cells through apoptosis. The objective of this study was to investigate whether the exposure to RF electromagnetic fields, similar to those emitted by mobile phones of the second generation standard, Global System for Mobile Communications (GSM), may induce expression of the p53 protein and its activation by post-translational modifications in cultured human cells. The potential induction of p53 expression and activation by GSM-900 was investigated after in vitro exposure of human amniotic cells for 24 h to average specific absorption rates (SARs) of 0.25, 1, 2, and 4 W/kg in the temperature range of 36.3-39.7 °C. The exposures were carried out using a wire-patch cell (WPC) under strictly controlled conditions of temperature. Expression and activation of p53 by phosphorylation at serine 15 and 37 were studied using Western blot assay immediately after three independent exposures of cell cultures provided from three different donors. Bleomycin-exposed cells were used as a positive control. According to our results, no significant changes in the expression and activation of the p53 protein by phosphorylation at serine 15 and 37 were found following exposure to GSM-900 for 24 h at average SARs up to 4 W/kg in human embryonic cells. Bioelectromagnetics. © 2012 Wiley Periodicals, Inc.</p> </div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="en">Study of p53 expression and post-transcriptional modifications after GSM-900 radiofrequency exposure of human amniotic cells</title> <author role="aut"> <persName> <forename type="first">Sylvie</forename> <surname>Bourthoumieu</surname> </persName> <email type="md5">58d54816d094f71844ce2a65927bc6ce</email> <email type="domain">unilim.fr</email> <idno type="halauthorid">609097</idno> <affiliation ref="#struct-155864"></affiliation> <affiliation ref="#struct-70831"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Amandine</forename> <surname>Magnaudeix</surname> </persName> <email type="md5">2403bca1beef77528e5f777f7a9fb8d0</email> <email type="domain">unilim.fr</email> <idno type="halauthorid">609323</idno> <affiliation ref="#struct-70831"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Faraj</forename> <surname>Terro</surname> </persName> <email type="md5">efbe792530c606c4c5a2915105c45a60</email> <email type="domain">unilim.fr</email> <idno type="halauthorid">609135</idno> <affiliation ref="#struct-155864"></affiliation> <affiliation ref="#struct-70831"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Philippe</forename> <surname>Lévêque</surname> </persName> <email type="md5">5301ec7109704fdaff372ed6026facf9</email> <email type="domain">unilim.fr</email> <idno type="idhal" notation="string">philippe-leveque</idno> <idno type="idhal" notation="numeric">6689</idno> <idno type="halauthorid">945512</idno> <affiliation ref="#struct-390935"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Alice</forename> <surname>Collin</surname> </persName> <idno type="halauthorid">188403</idno> </author> <author role="aut"> <persName> <forename type="first">Catherine</forename> <surname>Yardin</surname> </persName> <email type="md5">04313b726b78726dab7d7c8396326b2e</email> <email type="domain">unilim.fr</email> <idno type="idhal" notation="string">catherine-yardin</idno> <idno type="idhal" notation="numeric">180164</idno> <idno type="halauthorid">609098</idno> <idno type="ORCID">https://orcid.org/0000-0002-0049-3058</idno> <affiliation ref="#struct-203502"></affiliation> </author> <editor role="depositor"> <persName> <forename>Véronique</forename> <surname>Maury</surname> </persName> <email type="md5">a03fe93294649036fd171e6b8b30cd51</email> <email type="domain">xlim.fr</email> </editor> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2012-12-04 11:29:49</date> <date type="whenWritten">2012</date> <date type="whenModified">2020-01-13 09:14:04</date> <date type="whenReleased">2012-12-04 11:29:49</date> <date type="whenProduced">2013</date> <ref type="externalLink" target="https://api.istex.fr/ark:/67375/WNG-1M6526Z4-N/fulltext.pdf?sid=hal"></ref> </edition> <respStmt> <resp>contributor</resp> <name key="164456"> <persName> <forename>Véronique</forename> <surname>Maury</surname> </persName> <email type="md5">a03fe93294649036fd171e6b8b30cd51</email> <email type="domain">xlim.fr</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">hal-00760670</idno> <idno type="halUri">https://hal-unilim.archives-ouvertes.fr/hal-00760670</idno> <idno type="halBibtex">bourthoumieu:hal-00760670</idno> <idno type="halRefHtml">Bioelectromagnetics, Wiley, 2013, 34 (1), pp.52-60. ⟨10.1002/bem.21744⟩</idno> <idno type="halRef">Bioelectromagnetics, Wiley, 2013, 34 (1), pp.52-60. ⟨10.1002/bem.21744⟩</idno> </publicationStmt> <seriesStmt> <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno> <idno type="stamp" n="XLIM-OSA" corresp="UNILIM">Département OSA</idno> <idno type="stamp" n="UNILIM">Université de Limoges</idno> <idno type="stamp" n="XLIM" corresp="UNILIM">XLIM</idno> <idno type="stamp" n="EA3842" corresp="UNILIM">Homéostasie Cellulaire et Pathologies </idno> <idno type="stamp" n="ERMA" corresp="UNILIM">Equipe de Recherche Médicale Appliquée</idno> <idno type="stamp" n="GEIST" corresp="UNILIM">Génomique, environnement, immunité, thérapeutique</idno> <idno type="stamp" n="CAPTUR" corresp="UNILIM">Contrôle de l’Activation Cellulaire, Progression Tumorale et Résistance Thérapeutique</idno> </seriesStmt> <notesStmt> <note type="audience" n="2">International</note> <note type="popular" n="0">No</note> <note type="peer" n="1">Yes</note> </notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Study of p53 expression and post-transcriptional modifications after GSM-900 radiofrequency exposure of human amniotic cells</title> <author role="aut"> <persName> <forename type="first">Sylvie</forename> <surname>Bourthoumieu</surname> </persName> <email type="md5">58d54816d094f71844ce2a65927bc6ce</email> <email type="domain">unilim.fr</email> <idno type="halauthorid">609097</idno> <affiliation ref="#struct-155864"></affiliation> <affiliation ref="#struct-70831"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Amandine</forename> <surname>Magnaudeix</surname> </persName> <email type="md5">2403bca1beef77528e5f777f7a9fb8d0</email> <email type="domain">unilim.fr</email> <idno type="halauthorid">609323</idno> <affiliation ref="#struct-70831"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Faraj</forename> <surname>Terro</surname> </persName> <email type="md5">efbe792530c606c4c5a2915105c45a60</email> <email type="domain">unilim.fr</email> <idno type="halauthorid">609135</idno> <affiliation ref="#struct-155864"></affiliation> <affiliation ref="#struct-70831"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Philippe</forename> <surname>Lévêque</surname> </persName> <email type="md5">5301ec7109704fdaff372ed6026facf9</email> <email type="domain">unilim.fr</email> <idno type="idhal" notation="string">philippe-leveque</idno> <idno type="idhal" notation="numeric">6689</idno> <idno type="halauthorid">945512</idno> <affiliation ref="#struct-390935"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Alice</forename> <surname>Collin</surname> </persName> <idno type="halauthorid">188403</idno> </author> <author role="aut"> <persName> <forename type="first">Catherine</forename> <surname>Yardin</surname> </persName> <email type="md5">04313b726b78726dab7d7c8396326b2e</email> <email type="domain">unilim.fr</email> <idno type="idhal" notation="string">catherine-yardin</idno> <idno type="idhal" notation="numeric">180164</idno> <idno type="halauthorid">609098</idno> <idno type="ORCID">https://orcid.org/0000-0002-0049-3058</idno> <affiliation ref="#struct-203502"></affiliation> </author> </analytic> <monogr> <idno type="halJournalId" status="VALID">11142</idno> <idno type="issn">0197-8462</idno> <idno type="eissn">1521-186X</idno> <title level="j">Bioelectromagnetics</title> <imprint> <publisher>Wiley</publisher> <biblScope unit="volume">34</biblScope> <biblScope unit="issue">1</biblScope> <biblScope unit="pp">52-60</biblScope> <date type="datePub">2013</date> </imprint> </monogr> <idno type="doi">10.1002/bem.21744</idno> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="en">English</language> </langUsage> <textClass> <classCode scheme="halTypology" n="ART">Journal articles</classCode> </textClass> <abstract xml:lang="en"> <p>The potential effects of radiofrequency (RF) exposure on the genetic material of cells are very important to determine since genome instability of somatic cells may be linked to cancer development. In response to genetic damage, the p53 protein is activated and can induce cell cycle arrest allowing more time for DNA repair or elimination of damaged cells through apoptosis. The objective of this study was to investigate whether the exposure to RF electromagnetic fields, similar to those emitted by mobile phones of the second generation standard, Global System for Mobile Communications (GSM), may induce expression of the p53 protein and its activation by post-translational modifications in cultured human cells. The potential induction of p53 expression and activation by GSM-900 was investigated after in vitro exposure of human amniotic cells for 24 h to average specific absorption rates (SARs) of 0.25, 1, 2, and 4 W/kg in the temperature range of 36.3-39.7 °C. The exposures were carried out using a wire-patch cell (WPC) under strictly controlled conditions of temperature. Expression and activation of p53 by phosphorylation at serine 15 and 37 were studied using Western blot assay immediately after three independent exposures of cell cultures provided from three different donors. Bleomycin-exposed cells were used as a positive control. According to our results, no significant changes in the expression and activation of the p53 protein by phosphorylation at serine 15 and 37 were found following exposure to GSM-900 for 24 h at average SARs up to 4 W/kg in human embryonic cells. Bioelectromagnetics. © 2012 Wiley Periodicals, Inc.</p> </abstract> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
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