SrasV1, Hal, Checkpoint, bibRecord, 000199

Irradiation of fish embryos prior to blastomere transfer boosts the colonisation of their gonads by donor-derived gametes.

Identifieur interne : 000199 ( Hal/Checkpoint ); précédent : 000198; suivant : 000200

Irradiation of fish embryos prior to blastomere transfer boosts the colonisation of their gonads by donor-derived gametes.

Auteurs : Jean-Stéphane Joly [France] ; Chantal Kress [France] ; Marc Vandeputte [France] ; Franck Bourrat [France] ; Daniel Chourrout [France]

Source :

Molecular Reproduction and Development [ 1040-452X ] ; 1999-08.

RBID : Hal:hal-00123350

English descriptors

mix :
- cell transplantation, embryo, fish, germline chimeras, irradiation, medaka.

Abstract

Blastomere transplantation into fish blastula embryos results in somatic chimeras, which generally provide null or a small proportion of gametes derived from the donor. This may partly explain why none of the ES-like cell lines established from fish embryos has contributed to the germline of chimeras when transplanted at the blastula stage. Here, we report that a moderate gamma-irradiation of recipient embryos, followed by transplantation of dispersed blastomeres, considerably enhances the proportion of donor-derived gametes (53% versus 5% in average). In fish, the resulting protocol should maximise the pluripotency level measured in vivo for embryonic cell lines and for cultured germ cells.

Url:

https://hal.archives-ouvertes.fr/hal-00123350

DOI: 10.1002/(SICI)1098-2795(199908)53:4<394::AID-MRD4>3.0.CO;2-X

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000095
to stream Hal, to step Curation: 000095

Links to Exploration step

Hal:hal-00123350

Le document en format XML

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<front><div type="abstract" xml:lang="en">              <p>Blastomere transplantation into fish blastula embryos results in somatic chimeras, which generally provide null or a small proportion of gametes derived from the donor. This may partly explain why none of the ES-like cell lines established from fish embryos has contributed to the germline of chimeras when transplanted at the blastula stage. Here, we report that a moderate gamma-irradiation of recipient embryos, followed by transplantation of dispersed blastomeres, considerably enhances the proportion of donor-derived gametes (53% versus 5% in average). In fish, the resulting protocol should maximise the pluripotency level measured in vivo for embryonic cell lines and for cultured germ cells.</p>
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                <author role="aut">                  <persName>                    <forename type="first">Chantal</forename>
                    <surname>Kress</surname>
                  </persName>
                  <idno type="halauthorid">156143</idno>
                  <affiliation ref="#struct-27719"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Marc</forename>
                    <surname>Vandeputte</surname>
                  </persName>
                  <idno type="halauthorid">156144</idno>
                  <affiliation ref="#struct-27718"></affiliation>
                </author>
                <author role="crp">                  <persName>                    <forename type="first">Franck</forename>
                    <surname>Bourrat</surname>
                  </persName>
                  <email type="md5">45f30636095bda7ce7cca79ff57fa0b3</email>
                  <email type="domain">inaf.cnrs-gif.fr</email>
                  <idno type="halauthorid">154091</idno>
                  <affiliation ref="#struct-436"></affiliation>
                  <affiliation ref="#struct-27718"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Daniel</forename>
                    <surname>Chourrout</surname>
                  </persName>
                  <idno type="halauthorid">156130</idno>
                  <affiliation ref="#struct-27718"></affiliation>
                  <affiliation ref="#struct-27720"></affiliation>
                </author>
              </analytic>
              <monogr>                <idno type="halJournalId" status="VALID">17192</idno>
                <idno type="issn">1040-452X</idno>
                <idno type="eissn">1098-2795</idno>
                <title level="j">Molecular Reproduction and Development</title>
                <imprint>                  <publisher>Wiley</publisher>
                  <biblScope unit="volume">53</biblScope>
                  <biblScope unit="issue">4</biblScope>
                  <biblScope unit="pp">394-7</biblScope>
                  <date type="datePub">1999-08</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1002/(SICI)1098-2795(199908)53:4<394::AID-MRD4>3.0.CO;2-X</idno>
              <idno type="pubmed">10398414</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>            <langUsage>              <language ident="en">English</language>
            </langUsage>
            <textClass>              <keywords scheme="author">                <term xml:lang="en">fish</term>
                <term xml:lang="en">medaka</term>
                <term xml:lang="en">embryo</term>
                <term xml:lang="en">germline chimeras</term>
                <term xml:lang="en">cell transplantation</term>
                <term xml:lang="en">irradiation</term>
              </keywords>
              <classCode scheme="mesh">Animals</classCode>
              <classCode scheme="mesh">Blastomeres</classCode>
              <classCode scheme="mesh">Stem Cell Transplantation</classCode>
              <classCode scheme="mesh">Cell Transplantation</classCode>
              <classCode scheme="mesh">Female</classCode>
              <classCode scheme="mesh">Gamma Rays</classCode>
              <classCode scheme="mesh">Gonads</classCode>
              <classCode scheme="mesh">Male</classCode>
              <classCode scheme="mesh">Oryzias</classCode>
              <classCode scheme="mesh">Pigmentation</classCode>
              <classCode scheme="mesh">Radiation Chimera</classCode>
              <classCode scheme="halDomain" n="sdv.neu">Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">              <p>Blastomere transplantation into fish blastula embryos results in somatic chimeras, which generally provide null or a small proportion of gametes derived from the donor. This may partly explain why none of the ES-like cell lines established from fish embryos has contributed to the germline of chimeras when transplanted at the blastula stage. Here, we report that a moderate gamma-irradiation of recipient embryos, followed by transplantation of dispersed blastomeres, considerably enhances the proportion of donor-derived gametes (53% versus 5% in average). In fish, the resulting protocol should maximise the pluripotency level measured in vivo for embryonic cell lines and for cultured germ cells.</p>
            </abstract>
          </profileDesc>
        </hal>
</record>

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Irradiation of fish embryos prior to blastomere transfer boosts the colonisation of their gonads by donor-derived gametes.

Irradiation of fish embryos prior to blastomere transfer boosts the colonisation of their gonads by donor-derived gametes.

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