SrasV1, Hal, Checkpoint, bibRecord, 000192

The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world

Identifieur interne : 000192 ( Hal/Checkpoint ); précédent : 000191; suivant : 000193

The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world

Auteurs : Marie-Pierre Egloff ; Francois Ferron [France] ; Valérie Campanacci [France] ; Sonia Longhi [France] ; Corinne Rancurel [France] ; Hélène Dutartre [France] ; Eric Snijder [Pays-Bas] ; Alexander Gorbalenya [Pays-Bas] ; Christian Cambillau [France] ; Bruno Canard [France]

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2004-03-16.

RBID : Hal:hal-02459257

Abstract

The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to Coronaviridae (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we report the crystal structure at 2.7-A resolution of nsp9, a hitherto uncharacterized subunit of the SARS-CoV replicative polyproteins. We show that SARS-CoV nsp9 is a single-stranded RNA-binding protein displaying a previously unreported, oligosaccharide/oligonucleotide fold-like fold. The presence of this type of protein has not been detected in the replicative complexes of RNA viruses, and its presence may reflect the unique and complex CoV viral replication/transcription machinery.

Url:

https://hal.archives-ouvertes.fr/hal-02459257

DOI: 10.1073/pnas.0307877101

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000171
to stream Hal, to step Curation: 000171

Links to Exploration step

Hal:hal-02459257

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world</title>
<author><name sortKey="Egloff, Marie Pierre" sort="Egloff, Marie Pierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name>
</author>
<author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="Francois" last="Ferron">Francois Ferron</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-84651" status="VALID">          <orgName>Laboratoire d'Enzymologie et Biochimie Structurales</orgName>
          <orgName type="acronym">LEBS</orgName>
          <desc>            <address>              <addrLine>91 198 Gif-sur-Yvette Cedex</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation name="UPR3082" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UPR3082" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199347" status="OLD">          <orgName>Institut Sophia Agrobiotech [Sophia Antipolis]</orgName>
          <orgName type="acronym">ISA</orgName>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>INRA Centre de recherche Provence-Alpes-Côte d'Azur 400, route des Chappes BP 167 06903 Sophia Antipolis Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.paca.inra.fr/institut-sophia-agrobiotech</ref>
          </desc>
          <listRelation>            <relation name="UMR1355" active="#struct-92114" type="direct"></relation>
            <relation active="#struct-117617" type="direct"></relation>
            <relation active="#struct-523042" type="indirect"></relation>
            <relation name="UMR7254" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR1355" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-117617" type="direct"><org type="institution" xml:id="struct-117617" status="VALID">          <orgName>Université Nice Sophia Antipolis (... - 2019)</orgName>
          <orgName type="acronym">UNS</orgName>
          <desc>            <address>              <addrLine>Parc Valrose - BP 2135 - 06103 Nice cedex 2</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://unice.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-523042" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-523042" type="indirect"><org type="regroupinstitution" xml:id="struct-523042" status="VALID">          <orgName>Université Côte d'Azur </orgName>
          <orgName type="acronym">UCA</orgName>
          <date type="start">2015-03-01</date>
          <desc>            <address>              <addrLine>Parc Valrose, 28 avenue Valrose 06108 Nice Cedex 2 </addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7254" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Dutartre, Helene" sort="Dutartre, Helene" uniqKey="Dutartre H" first="Hélène" last="Dutartre">Hélène Dutartre</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-543605" status="VALID">          <orgName>Oncogenèse rétrovirale – Retroviral Oncogenesis</orgName>
          <date type="start">2013-01-01</date>
          <desc>            <address>              <addrLine>Equipe “Oncogénèse rétrovirale”CIRI – U1111 / UMR5308Ecole Normale Supérieure de Lyon1er étage – Bâtiment LR546 allée d’Italie69007 Lyon</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ciri.inserm.fr</ref>
          </desc>
          <listRelation>            <relation active="#struct-222995" type="direct"></relation>
            <relation name="U1111" active="#struct-303623" type="indirect"></relation>
            <relation active="#struct-6818" type="indirect"></relation>
            <relation active="#struct-194495" type="indirect"></relation>
            <relation active="#struct-301088" type="indirect"></relation>
            <relation name="UMR5308" active="#struct-441569" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-222995" type="direct"><org type="regrouplaboratory" xml:id="struct-222995" status="VALID">          <idno type="IdRef">17825696X</idno>
          <idno type="RNSR">201320572J</idno>
          <orgName>Centre International de Recherche en Infectiologie - UMR</orgName>
          <orgName type="acronym">CIRI</orgName>
          <date type="start">2013-01-01</date>
          <desc>            <address>              <addrLine>21 avenue Tony Garnier 69365 Lyon Cedex 07</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ciri.inserm.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-6818" type="direct"></relation>
            <relation active="#struct-194495" type="direct"></relation>
            <relation active="#struct-301088" type="indirect"></relation>
            <relation name="U1111" active="#struct-303623" type="direct"></relation>
            <relation name="UMR5308" active="#struct-441569" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle name="U1111" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID">          <idno type="IdRef">026388278</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>            <address>              <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-6818" type="indirect"><org type="institution" xml:id="struct-6818" status="VALID">          <idno type="IdRef">149154992</idno>
          <orgName>École normale supérieure - Lyon</orgName>
          <orgName type="acronym">ENS Lyon</orgName>
          <desc>            <address>              <addrLine>15 parvis René Descartes - BP 7000 - 69342 Lyon Cedex 07</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ens-lyon.eu/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-194495" type="indirect"><org type="institution" xml:id="struct-194495" status="VALID">          <idno type="IdRef">026402823</idno>
          <idno type="ISNI">0000000121686185</idno>
          <orgName>Université Claude Bernard Lyon 1</orgName>
          <orgName type="acronym">UCBL</orgName>
          <desc>            <address>              <addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-lyon1.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-301088" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-301088" type="indirect"><org type="regroupinstitution" xml:id="struct-301088" status="VALID">          <orgName>Université de Lyon</orgName>
          <desc>            <address>              <addrLine>92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">https://www.universite-lyon.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR5308" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Lyon</settlement>
<region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
</placeName>
<orgName type="university">Université Claude Bernard Lyon 1</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Lyon</orgName>
</affiliation>
</author>
<author><name sortKey="Snijder, Eric" sort="Snijder, Eric" uniqKey="Snijder E" first="Eric" last="Snijder">Eric Snijder</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300851" status="VALID">          <orgName>Leiden University Medical Center (LUMC)</orgName>
          <desc>            <address>              <country key="NL"></country>
            </address>
          </desc>
        </hal:affiliation>
<country>Pays-Bas</country>
</affiliation>
</author>
<author><name sortKey="Gorbalenya, Alexander" sort="Gorbalenya, Alexander" uniqKey="Gorbalenya A" first="Alexander" last="Gorbalenya">Alexander Gorbalenya</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300851" status="VALID">          <orgName>Leiden University Medical Center (LUMC)</orgName>
          <desc>            <address>              <country key="NL"></country>
            </address>
          </desc>
        </hal:affiliation>
<country>Pays-Bas</country>
</affiliation>
</author>
<author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-02459257</idno>
<idno type="halId">hal-02459257</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-02459257</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-02459257</idno>
<idno type="doi">10.1073/pnas.0307877101</idno>
<date when="2004-03-16">2004-03-16</date>
<idno type="wicri:Area/Hal/Corpus">000171</idno>
<idno type="wicri:Area/Hal/Curation">000171</idno>
<idno type="wicri:Area/Hal/Checkpoint">000192</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000192</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world</title>
<author><name sortKey="Egloff, Marie Pierre" sort="Egloff, Marie Pierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name>
</author>
<author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="Francois" last="Ferron">Francois Ferron</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-84651" status="VALID">          <orgName>Laboratoire d'Enzymologie et Biochimie Structurales</orgName>
          <orgName type="acronym">LEBS</orgName>
          <desc>            <address>              <addrLine>91 198 Gif-sur-Yvette Cedex</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation name="UPR3082" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UPR3082" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199347" status="OLD">          <orgName>Institut Sophia Agrobiotech [Sophia Antipolis]</orgName>
          <orgName type="acronym">ISA</orgName>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>INRA Centre de recherche Provence-Alpes-Côte d'Azur 400, route des Chappes BP 167 06903 Sophia Antipolis Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.paca.inra.fr/institut-sophia-agrobiotech</ref>
          </desc>
          <listRelation>            <relation name="UMR1355" active="#struct-92114" type="direct"></relation>
            <relation active="#struct-117617" type="direct"></relation>
            <relation active="#struct-523042" type="indirect"></relation>
            <relation name="UMR7254" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR1355" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-117617" type="direct"><org type="institution" xml:id="struct-117617" status="VALID">          <orgName>Université Nice Sophia Antipolis (... - 2019)</orgName>
          <orgName type="acronym">UNS</orgName>
          <desc>            <address>              <addrLine>Parc Valrose - BP 2135 - 06103 Nice cedex 2</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://unice.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-523042" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-523042" type="indirect"><org type="regroupinstitution" xml:id="struct-523042" status="VALID">          <orgName>Université Côte d'Azur </orgName>
          <orgName type="acronym">UCA</orgName>
          <date type="start">2015-03-01</date>
          <desc>            <address>              <addrLine>Parc Valrose, 28 avenue Valrose 06108 Nice Cedex 2 </addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7254" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Dutartre, Helene" sort="Dutartre, Helene" uniqKey="Dutartre H" first="Hélène" last="Dutartre">Hélène Dutartre</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-543605" status="VALID">          <orgName>Oncogenèse rétrovirale – Retroviral Oncogenesis</orgName>
          <date type="start">2013-01-01</date>
          <desc>            <address>              <addrLine>Equipe “Oncogénèse rétrovirale”CIRI – U1111 / UMR5308Ecole Normale Supérieure de Lyon1er étage – Bâtiment LR546 allée d’Italie69007 Lyon</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ciri.inserm.fr</ref>
          </desc>
          <listRelation>            <relation active="#struct-222995" type="direct"></relation>
            <relation name="U1111" active="#struct-303623" type="indirect"></relation>
            <relation active="#struct-6818" type="indirect"></relation>
            <relation active="#struct-194495" type="indirect"></relation>
            <relation active="#struct-301088" type="indirect"></relation>
            <relation name="UMR5308" active="#struct-441569" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-222995" type="direct"><org type="regrouplaboratory" xml:id="struct-222995" status="VALID">          <idno type="IdRef">17825696X</idno>
          <idno type="RNSR">201320572J</idno>
          <orgName>Centre International de Recherche en Infectiologie - UMR</orgName>
          <orgName type="acronym">CIRI</orgName>
          <date type="start">2013-01-01</date>
          <desc>            <address>              <addrLine>21 avenue Tony Garnier 69365 Lyon Cedex 07</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ciri.inserm.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-6818" type="direct"></relation>
            <relation active="#struct-194495" type="direct"></relation>
            <relation active="#struct-301088" type="indirect"></relation>
            <relation name="U1111" active="#struct-303623" type="direct"></relation>
            <relation name="UMR5308" active="#struct-441569" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle name="U1111" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID">          <idno type="IdRef">026388278</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>            <address>              <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-6818" type="indirect"><org type="institution" xml:id="struct-6818" status="VALID">          <idno type="IdRef">149154992</idno>
          <orgName>École normale supérieure - Lyon</orgName>
          <orgName type="acronym">ENS Lyon</orgName>
          <desc>            <address>              <addrLine>15 parvis René Descartes - BP 7000 - 69342 Lyon Cedex 07</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ens-lyon.eu/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-194495" type="indirect"><org type="institution" xml:id="struct-194495" status="VALID">          <idno type="IdRef">026402823</idno>
          <idno type="ISNI">0000000121686185</idno>
          <orgName>Université Claude Bernard Lyon 1</orgName>
          <orgName type="acronym">UCBL</orgName>
          <desc>            <address>              <addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-lyon1.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-301088" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-301088" type="indirect"><org type="regroupinstitution" xml:id="struct-301088" status="VALID">          <orgName>Université de Lyon</orgName>
          <desc>            <address>              <addrLine>92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">https://www.universite-lyon.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR5308" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Lyon</settlement>
<region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
</placeName>
<orgName type="university">Université Claude Bernard Lyon 1</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Lyon</orgName>
</affiliation>
</author>
<author><name sortKey="Snijder, Eric" sort="Snijder, Eric" uniqKey="Snijder E" first="Eric" last="Snijder">Eric Snijder</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300851" status="VALID">          <orgName>Leiden University Medical Center (LUMC)</orgName>
          <desc>            <address>              <country key="NL"></country>
            </address>
          </desc>
        </hal:affiliation>
<country>Pays-Bas</country>
</affiliation>
</author>
<author><name sortKey="Gorbalenya, Alexander" sort="Gorbalenya, Alexander" uniqKey="Gorbalenya A" first="Alexander" last="Gorbalenya">Alexander Gorbalenya</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300851" status="VALID">          <orgName>Leiden University Medical Center (LUMC)</orgName>
          <desc>            <address>              <country key="NL"></country>
            </address>
          </desc>
        </hal:affiliation>
<country>Pays-Bas</country>
</affiliation>
</author>
<author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1073/pnas.0307877101</idno>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America </title>
<idno type="ISSN">0027-8424</idno>
<imprint><date type="datePub">2004-03-16</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to Coronaviridae (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we report the crystal structure at 2.7-A resolution of nsp9, a hitherto uncharacterized subunit of the SARS-CoV replicative polyproteins. We show that SARS-CoV nsp9 is a single-stranded RNA-binding protein displaying a previously unreported, oligosaccharide/oligonucleotide fold-like fold. The presence of this type of protein has not been detected in the replicative complexes of RNA viruses, and its presence may reflect the unique and complex CoV viral replication/transcription machinery.</p>
            </div>
</front>
</TEI>
<hal api="V3">          <titleStmt>            <title xml:lang="en">The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world</title>
            <author role="aut">              <persName>                <forename type="first">Marie-Pierre</forename>
                <surname>Egloff</surname>
              </persName>
              <idno type="halauthorid">11434122</idno>
            </author>
            <author role="aut">              <persName>                <forename type="first">Francois</forename>
                <surname>Ferron</surname>
              </persName>
              <idno type="halauthorid">1458032</idno>
              <affiliation ref="#struct-199349"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Valérie</forename>
                <surname>Campanacci</surname>
              </persName>
              <idno type="halauthorid">234470</idno>
              <affiliation ref="#struct-84651"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Sonia</forename>
                <surname>Longhi</surname>
              </persName>
              <idno type="halauthorid">212576</idno>
              <affiliation ref="#struct-199349"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Corinne</forename>
                <surname>Rancurel</surname>
              </persName>
              <idno type="halauthorid">176470</idno>
              <affiliation ref="#struct-199347"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Hélène</forename>
                <surname>Dutartre</surname>
              </persName>
              <idno type="halauthorid">434484</idno>
              <affiliation ref="#struct-543605"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Eric</forename>
                <forename type="middle">J</forename>
                <surname>Snijder</surname>
              </persName>
              <idno type="halauthorid">11637305</idno>
              <affiliation ref="#struct-300851"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Alexander</forename>
                <surname>Gorbalenya</surname>
              </persName>
              <idno type="halauthorid">11385846</idno>
              <affiliation ref="#struct-300851"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Christian</forename>
                <surname>Cambillau</surname>
              </persName>
              <idno type="halauthorid">234469</idno>
              <affiliation ref="#struct-199349"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Bruno</forename>
                <surname>Canard</surname>
              </persName>
              <email type="md5">b082b648c100da933978ce65df2a1ad4</email>
              <email type="domain">afmb.univ-mrs.fr</email>
              <idno type="halauthorid">728889</idno>
              <affiliation ref="#struct-199349"></affiliation>
            </author>
            <editor role="depositor">              <persName>                <forename>Helene</forename>
                <surname>Dutartre</surname>
              </persName>
              <email type="md5">e9950f5e032f5510dd65c5ed2f354324</email>
              <email type="domain">ens-lyon.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>            <edition n="v1" type="current">              <date type="whenSubmitted">2020-01-29 11:52:47</date>
              <date type="whenModified">2020-03-17 03:35:45</date>
              <date type="whenReleased">2020-01-29 11:52:47</date>
              <date type="whenProduced">2004-03-16</date>
              <ref type="externalLink" target="https://www.pnas.org/content/pnas/101/11/3792.full.pdf"></ref>
            </edition>
            <respStmt>              <resp>contributor</resp>
              <name key="41154">                <persName>                  <forename>Helene</forename>
                  <surname>Dutartre</surname>
                </persName>
                <email type="md5">e9950f5e032f5510dd65c5ed2f354324</email>
                <email type="domain">ens-lyon.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>            <distributor>CCSD</distributor>
            <idno type="halId">hal-02459257</idno>
            <idno type="halUri">https://hal.archives-ouvertes.fr/hal-02459257</idno>
            <idno type="halBibtex">egloff:hal-02459257</idno>
            <idno type="halRefHtml">Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2004, 101 (11), pp.3792-3796. ⟨10.1073/pnas.0307877101⟩</idno>
            <idno type="halRef">Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2004, 101 (11), pp.3792-3796. ⟨10.1073/pnas.0307877101⟩</idno>
          </publicationStmt>
          <seriesStmt>            <idno type="stamp" n="INRA">INRA - Institut national de la recherche agronomique</idno>
            <idno type="stamp" n="ENS-LYON">École Normale Supérieure de Lyon</idno>
            <idno type="stamp" n="UNIV-LYON1">Université Claude Bernard - Lyon I</idno>
            <idno type="stamp" n="UNICE">Université Nice Sophia Antipolis</idno>
            <idno type="stamp" n="UCA-TEST">UCA-TEST</idno>
            <idno type="stamp" n="UNIV-COTEDAZUR">Université Côte d'Azur</idno>
            <idno type="stamp" n="CIRI" corresp="UNIV-LYON1">Centre International de Recherche en Infectiologie</idno>
            <idno type="stamp" n="UNIV-AMU">Aix Marseille Université</idno>
            <idno type="stamp" n="TEST-AMU">Espace de test AMU</idno>
            <idno type="stamp" n="CIRI-ONCOGENESE-RETROVIRALE" corresp="CIRI">CIRI-Oncogenèse rétrovirale-Retroviral Oncogenesis</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UDL">UDL</idno>
            <idno type="stamp" n="UNIV-LYON">Université de Lyon</idno>
            <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
          </seriesStmt>
          <notesStmt>            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>            <biblStruct>              <analytic>                <title xml:lang="en">The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world</title>
                <author role="aut">                  <persName>                    <forename type="first">Marie-Pierre</forename>
                    <surname>Egloff</surname>
                  </persName>
                  <idno type="halauthorid">11434122</idno>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Francois</forename>
                    <surname>Ferron</surname>
                  </persName>
                  <idno type="halauthorid">1458032</idno>
                  <affiliation ref="#struct-199349"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Valérie</forename>
                    <surname>Campanacci</surname>
                  </persName>
                  <idno type="halauthorid">234470</idno>
                  <affiliation ref="#struct-84651"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Sonia</forename>
                    <surname>Longhi</surname>
                  </persName>
                  <idno type="halauthorid">212576</idno>
                  <affiliation ref="#struct-199349"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Corinne</forename>
                    <surname>Rancurel</surname>
                  </persName>
                  <idno type="halauthorid">176470</idno>
                  <affiliation ref="#struct-199347"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Hélène</forename>
                    <surname>Dutartre</surname>
                  </persName>
                  <idno type="halauthorid">434484</idno>
                  <affiliation ref="#struct-543605"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Eric</forename>
                    <forename type="middle">J</forename>
                    <surname>Snijder</surname>
                  </persName>
                  <idno type="halauthorid">11637305</idno>
                  <affiliation ref="#struct-300851"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Alexander</forename>
                    <surname>Gorbalenya</surname>
                  </persName>
                  <idno type="halauthorid">11385846</idno>
                  <affiliation ref="#struct-300851"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Christian</forename>
                    <surname>Cambillau</surname>
                  </persName>
                  <idno type="halauthorid">234469</idno>
                  <affiliation ref="#struct-199349"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Bruno</forename>
                    <surname>Canard</surname>
                  </persName>
                  <email type="md5">b082b648c100da933978ce65df2a1ad4</email>
                  <email type="domain">afmb.univ-mrs.fr</email>
                  <idno type="halauthorid">728889</idno>
                  <affiliation ref="#struct-199349"></affiliation>
                </author>
              </analytic>
              <monogr>                <idno type="halJournalId" status="VALID">7969</idno>
                <idno type="issn">0027-8424</idno>
                <idno type="eissn">1091-6490</idno>
                <title level="j">Proceedings of the National Academy of Sciences of the United States of America </title>
                <imprint>                  <publisher>National Academy of Sciences</publisher>
                  <biblScope unit="volume">101</biblScope>
                  <biblScope unit="issue">11</biblScope>
                  <biblScope unit="pp">3792-3796</biblScope>
                  <date type="datePub">2004-03-16</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1073/pnas.0307877101</idno>
              <idno type="pubmed">15007178</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>            <langUsage>              <language ident="en">English</language>
            </langUsage>
            <textClass>              <classCode scheme="halDomain" n="sdv.mp.vir">Life Sciences [q-bio]/Microbiology and Parasitology/Virology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">              <p>The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to Coronaviridae (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we report the crystal structure at 2.7-A resolution of nsp9, a hitherto uncharacterized subunit of the SARS-CoV replicative polyproteins. We show that SARS-CoV nsp9 is a single-stranded RNA-binding protein displaying a previously unreported, oligosaccharide/oligonucleotide fold-like fold. The presence of this type of protein has not been detected in the replicative complexes of RNA viruses, and its presence may reflect the unique and complex CoV viral replication/transcription machinery.</p>
            </abstract>
          </profileDesc>
        </hal>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Hal/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000192 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000192 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Hal
   |étape=   Checkpoint
   |type=    RBID
   |clé=     Hal:hal-02459257
   |texte=   The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world

The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri