SrasV1, Hal, Checkpoint, bibRecord, 000124

Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.

Identifieur interne : 000124 ( Hal/Checkpoint ); précédent : 000123; suivant : 000125

Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.

Auteurs : Claire Debarnot [France] ; Isabelle Imbert ; François Ferron ; Laure Gluais ; Isabelle Varlet [France] ; Nicolas Papageorgiou ; Mickaël Bouvet [France] ; Julien Lescar ; Etienne Decroly [France] ; Bruno Canard

Source :

Acta crystallographica. Section F, Structural biology communications [ 2053-230X ] ; 2011-03-01.

RBID : Hal:hal-00751530

Abstract

To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.

Url:

https://hal.archives-ouvertes.fr/hal-00751530

DOI: 10.1107/S1744309111002867

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000042
to stream Hal, to step Curation: 000042

Links to Exploration step

Hal:hal-00751530

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.</title>
<author><name sortKey="Debarnot, Claire" sort="Debarnot, Claire" uniqKey="Debarnot C" first="Claire" last="Debarnot">Claire Debarnot</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192792" status="OLD">          <idno type="IdRef">141380888</idno>
          <idno type="RNSR">201220261A</idno>
          <orgName>Centre de recherche en neurobiologie - neurophysiologie de Marseille</orgName>
          <orgName type="acronym">CRN2M</orgName>
          <date type="start">2012</date>
          <date type="end">2017-12-31</date>
          <desc>            <address>              <addrLine>Faculté de Médecine Secteur Nord Boulevard Pierre Dramard 13916 MARSEILLE CEDEX 20</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://crn2m.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7286" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7286" active="#struct-303623" type="direct"></relation>
            <relation name="UMR7286" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7286" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7286" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID">          <idno type="IdRef">026388278</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>            <address>              <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7286" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name>
</author>
<author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name>
</author>
<author><name sortKey="Gluais, Laure" sort="Gluais, Laure" uniqKey="Gluais L" first="Laure" last="Gluais">Laure Gluais</name>
</author>
<author><name sortKey="Varlet, Isabelle" sort="Varlet, Isabelle" uniqKey="Varlet I" first="Isabelle" last="Varlet">Isabelle Varlet</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name>
</author>
<author><name sortKey="Bouvet, Mickael" sort="Bouvet, Mickael" uniqKey="Bouvet M" first="Mickaël" last="Bouvet">Mickaël Bouvet</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-58574" status="OLD">          <orgName>Division Eau et Environnement</orgName>
          <orgName type="acronym">LCPC/EAU</orgName>
          <desc>            <address>              <addrLine>Centre de Nantes Route de Bouaye - BP 4129 44341 Bouguenais Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.lcpc.fr/fr/presentation/organigramme/div_eau/</ref>
          </desc>
          <listRelation>            <relation active="#struct-13621" type="direct"></relation>
            <relation active="#struct-301358" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-13621" type="direct"><org type="institution" xml:id="struct-13621" status="OLD">          <orgName>Laboratoire Central des Ponts et Chaussées</orgName>
          <orgName type="acronym">LCPC</orgName>
          <desc>            <address>              <addrLine>58 boulevard Lefebvre 75732 PARIS Cedex 15</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.lcpc.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-301358" type="direct"><org type="regroupinstitution" xml:id="struct-301358" status="OLD">          <idno type="IdRef">187401039</idno>
          <orgName>PRES Université Nantes Angers Le Mans</orgName>
          <orgName type="acronym">UNAM</orgName>
          <date type="start">2008-12-31</date>
          <date type="end">2016-01-01</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Lescar, Julien" sort="Lescar, Julien" uniqKey="Lescar J" first="Julien" last="Lescar">Julien Lescar</name>
</author>
<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-00751530</idno>
<idno type="halId">hal-00751530</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-00751530</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-00751530</idno>
<idno type="doi">10.1107/S1744309111002867</idno>
<date when="2011-03-01">2011-03-01</date>
<idno type="wicri:Area/Hal/Corpus">000042</idno>
<idno type="wicri:Area/Hal/Curation">000042</idno>
<idno type="wicri:Area/Hal/Checkpoint">000124</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000124</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.</title>
<author><name sortKey="Debarnot, Claire" sort="Debarnot, Claire" uniqKey="Debarnot C" first="Claire" last="Debarnot">Claire Debarnot</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192792" status="OLD">          <idno type="IdRef">141380888</idno>
          <idno type="RNSR">201220261A</idno>
          <orgName>Centre de recherche en neurobiologie - neurophysiologie de Marseille</orgName>
          <orgName type="acronym">CRN2M</orgName>
          <date type="start">2012</date>
          <date type="end">2017-12-31</date>
          <desc>            <address>              <addrLine>Faculté de Médecine Secteur Nord Boulevard Pierre Dramard 13916 MARSEILLE CEDEX 20</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://crn2m.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7286" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7286" active="#struct-303623" type="direct"></relation>
            <relation name="UMR7286" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7286" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7286" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID">          <idno type="IdRef">026388278</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>            <address>              <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7286" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name>
</author>
<author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name>
</author>
<author><name sortKey="Gluais, Laure" sort="Gluais, Laure" uniqKey="Gluais L" first="Laure" last="Gluais">Laure Gluais</name>
</author>
<author><name sortKey="Varlet, Isabelle" sort="Varlet, Isabelle" uniqKey="Varlet I" first="Isabelle" last="Varlet">Isabelle Varlet</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name>
</author>
<author><name sortKey="Bouvet, Mickael" sort="Bouvet, Mickael" uniqKey="Bouvet M" first="Mickaël" last="Bouvet">Mickaël Bouvet</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-58574" status="OLD">          <orgName>Division Eau et Environnement</orgName>
          <orgName type="acronym">LCPC/EAU</orgName>
          <desc>            <address>              <addrLine>Centre de Nantes Route de Bouaye - BP 4129 44341 Bouguenais Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.lcpc.fr/fr/presentation/organigramme/div_eau/</ref>
          </desc>
          <listRelation>            <relation active="#struct-13621" type="direct"></relation>
            <relation active="#struct-301358" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-13621" type="direct"><org type="institution" xml:id="struct-13621" status="OLD">          <orgName>Laboratoire Central des Ponts et Chaussées</orgName>
          <orgName type="acronym">LCPC</orgName>
          <desc>            <address>              <addrLine>58 boulevard Lefebvre 75732 PARIS Cedex 15</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.lcpc.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-301358" type="direct"><org type="regroupinstitution" xml:id="struct-301358" status="OLD">          <idno type="IdRef">187401039</idno>
          <orgName>PRES Université Nantes Angers Le Mans</orgName>
          <orgName type="acronym">UNAM</orgName>
          <date type="start">2008-12-31</date>
          <date type="end">2016-01-01</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Lescar, Julien" sort="Lescar, Julien" uniqKey="Lescar J" first="Julien" last="Lescar">Julien Lescar</name>
</author>
<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
            <relation name="UMR7257" active="#struct-198056" type="direct"></relation>
            <relation name="UMR7257" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD">          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
</author>
</analytic>
<idno type="DOI">10.1107/S1744309111002867</idno>
<series><title level="j">Acta crystallographica. Section F, Structural biology communications</title>
<idno type="ISSN">2053-230X</idno>
<imprint><date type="datePub">2011-03-01</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.</p>
            </div>
</front>
</TEI>
<hal api="V3">          <titleStmt>            <title xml:lang="en">Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.</title>
            <author role="aut">              <persName>                <forename type="first">Claire</forename>
                <surname>Debarnot</surname>
              </persName>
              <idno type="halauthorid">434481</idno>
              <affiliation ref="#struct-192792"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Isabelle</forename>
                <surname>Imbert</surname>
              </persName>
              <idno type="halauthorid">782731</idno>
            </author>
            <author role="aut">              <persName>                <forename type="first">François</forename>
                <surname>Ferron</surname>
              </persName>
              <idno type="halauthorid">782732</idno>
            </author>
            <author role="aut">              <persName>                <forename type="first">Laure</forename>
                <surname>Gluais</surname>
              </persName>
              <idno type="halauthorid">782733</idno>
            </author>
            <author role="aut">              <persName>                <forename type="first">Isabelle</forename>
                <surname>Varlet</surname>
              </persName>
              <email type="md5">0681ea4b0e59b2b3df57380b0b9940d9</email>
              <email type="domain">univ-amu.fr</email>
              <idno type="idhal" notation="string">isabelle-varlet</idno>
              <idno type="idhal" notation="numeric">182778</idno>
              <idno type="halauthorid">763204</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-1711-7980</idno>
              <affiliation ref="#struct-199349"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Nicolas</forename>
                <surname>Papageorgiou</surname>
              </persName>
              <idno type="halauthorid">782734</idno>
            </author>
            <author role="aut">              <persName>                <forename type="first">Mickaël</forename>
                <surname>Bouvet</surname>
              </persName>
              <idno type="halauthorid">782735</idno>
              <affiliation ref="#struct-58574"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Julien</forename>
                <surname>Lescar</surname>
              </persName>
              <idno type="halauthorid">174764</idno>
            </author>
            <author role="aut">              <persName>                <forename type="first">Etienne</forename>
                <surname>Decroly</surname>
              </persName>
              <email type="md5">5d4b15d0e684f3ec48f16b6e5eaefe49</email>
              <email type="domain">afmb.univ-mrs.fr</email>
              <idno type="idhal" notation="string">etienne-decroly</idno>
              <idno type="idhal" notation="numeric">178424</idno>
              <idno type="halauthorid">726902</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-6046-024X</idno>
              <affiliation ref="#struct-199349"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Bruno</forename>
                <surname>Canard</surname>
              </persName>
              <idno type="halauthorid">429455</idno>
            </author>
            <editor role="depositor">              <persName>                <forename>Corinne</forename>
                <surname>Dumonceaud</surname>
              </persName>
              <email type="md5">f18045acd4445aa0c01ac62502418281</email>
              <email type="domain">univmed.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>            <edition n="v1" type="current">              <date type="whenSubmitted">2012-11-13 17:26:06</date>
              <date type="whenModified">2020-03-17 03:47:16</date>
              <date type="whenReleased">2012-11-13 17:26:06</date>
              <date type="whenProduced">2011-03-01</date>
              <ref type="externalLink" target="http://journals.iucr.org/f/issues/2011/03/00/nj5077/nj5077.pdf"></ref>
            </edition>
            <respStmt>              <resp>contributor</resp>
              <name key="108516">                <persName>                  <forename>Corinne</forename>
                  <surname>Dumonceaud</surname>
                </persName>
                <email type="md5">f18045acd4445aa0c01ac62502418281</email>
                <email type="domain">univmed.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>            <distributor>CCSD</distributor>
            <idno type="halId">hal-00751530</idno>
            <idno type="halUri">https://hal.archives-ouvertes.fr/hal-00751530</idno>
            <idno type="halBibtex">debarnot:hal-00751530</idno>
            <idno type="halRefHtml">Acta crystallographica. Section F, Structural biology communications, John Wiley & Sons Ltd,, 2011, 67 (Pt 3), pp.404-8. ⟨10.1107/S1744309111002867⟩</idno>
            <idno type="halRef">Acta crystallographica. Section F, Structural biology communications, John Wiley & Sons Ltd,, 2011, 67 (Pt 3), pp.404-8. ⟨10.1107/S1744309111002867⟩</idno>
          </publicationStmt>
          <seriesStmt>            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNAM">l'unam - université nantes angers le mans</idno>
            <idno type="stamp" n="UNIV-AMU">Aix Marseille Université</idno>
            <idno type="stamp" n="IFSTTAR">Ifsttar</idno>
            <idno type="stamp" n="INRA">INRA - Institut national de la recherche agronomique</idno>
            <idno type="stamp" n="TEST-AMU">Espace de test AMU</idno>
            <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
          </seriesStmt>
          <notesStmt>            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>            <biblStruct>              <analytic>                <title xml:lang="en">Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.</title>
                <author role="aut">                  <persName>                    <forename type="first">Claire</forename>
                    <surname>Debarnot</surname>
                  </persName>
                  <idno type="halauthorid">434481</idno>
                  <affiliation ref="#struct-192792"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Isabelle</forename>
                    <surname>Imbert</surname>
                  </persName>
                  <idno type="halauthorid">782731</idno>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">François</forename>
                    <surname>Ferron</surname>
                  </persName>
                  <idno type="halauthorid">782732</idno>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Laure</forename>
                    <surname>Gluais</surname>
                  </persName>
                  <idno type="halauthorid">782733</idno>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Isabelle</forename>
                    <surname>Varlet</surname>
                  </persName>
                  <email type="md5">0681ea4b0e59b2b3df57380b0b9940d9</email>
                  <email type="domain">univ-amu.fr</email>
                  <idno type="idhal" notation="string">isabelle-varlet</idno>
                  <idno type="idhal" notation="numeric">182778</idno>
                  <idno type="halauthorid">763204</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-1711-7980</idno>
                  <affiliation ref="#struct-199349"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Nicolas</forename>
                    <surname>Papageorgiou</surname>
                  </persName>
                  <idno type="halauthorid">782734</idno>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Mickaël</forename>
                    <surname>Bouvet</surname>
                  </persName>
                  <idno type="halauthorid">782735</idno>
                  <affiliation ref="#struct-58574"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Julien</forename>
                    <surname>Lescar</surname>
                  </persName>
                  <idno type="halauthorid">174764</idno>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Etienne</forename>
                    <surname>Decroly</surname>
                  </persName>
                  <email type="md5">5d4b15d0e684f3ec48f16b6e5eaefe49</email>
                  <email type="domain">afmb.univ-mrs.fr</email>
                  <idno type="idhal" notation="string">etienne-decroly</idno>
                  <idno type="idhal" notation="numeric">178424</idno>
                  <idno type="halauthorid">726902</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-6046-024X</idno>
                  <affiliation ref="#struct-199349"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Bruno</forename>
                    <surname>Canard</surname>
                  </persName>
                  <idno type="halauthorid">429455</idno>
                </author>
              </analytic>
              <monogr>                <idno type="halJournalId" status="VALID">102256</idno>
                <idno type="issn">2053-230X</idno>
                <title level="j">Acta crystallographica. Section F, Structural biology communications</title>
                <imprint>                  <publisher>John Wiley & Sons Ltd,</publisher>
                  <biblScope unit="volume">67</biblScope>
                  <biblScope unit="issue">Pt 3</biblScope>
                  <biblScope unit="pp">404-8</biblScope>
                  <date type="datePub">2011-03-01</date>
                  <date type="dateEpub">2011-02-25</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1107/S1744309111002867</idno>
              <idno type="pubmed">21393853</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>            <langUsage>              <language ident="en">English</language>
            </langUsage>
            <textClass>              <classCode scheme="mesh">Cloning, Molecular</classCode>
              <classCode scheme="mesh">Crystallization</classCode>
              <classCode scheme="mesh">Crystallography, X-Ray</classCode>
              <classCode scheme="mesh">Humans</classCode>
              <classCode scheme="mesh">Methyltransferases</classCode>
              <classCode scheme="mesh">Molecular Sequence Data</classCode>
              <classCode scheme="mesh">RNA Replicase</classCode>
              <classCode scheme="mesh">SARS Virus</classCode>
              <classCode scheme="mesh">Viral Nonstructural Proteins</classCode>
              <classCode scheme="halDomain" n="sdv.bbm.bc">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">              <p>To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.</p>
            </abstract>
          </profileDesc>
        </hal>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Hal/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000124 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000124 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Hal
   |étape=   Checkpoint
   |type=    RBID
   |clé=     Hal:hal-00751530
   |texte=   Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.

Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri