SrasV1, France, Analysis, bibRecord, 000214

Scanless two-photon excitation of channelrhodopsin-2

Identifieur interne : 000214 ( France/Analysis ); précédent : 000213; suivant : 000215

Scanless two-photon excitation of channelrhodopsin-2

Auteurs : Eirini Papagiakoumou [France] ; Francesca Anselmi [France] ; Aurélien Bègue [France] ; Vincent De Sars [France] ; Jesper Glückstad [Danemark] ; Ehud Isacoff [États-Unis] ; Valentina Emiliani [France]

Source :

Nature Methods [ 1548-7091 ] ; 2010.

RBID : Hal:hal-01963417

Abstract

Light-gated ion channels and pumps have made it possible to probe intact neural circuits by manipulating the activity of groups of genetically similar neurons. What is needed now is a method for precisely aiming the stimulating light at single neuronal processes, neurons or groups of neurons. We developed a method that combines generalized phase contrast with temporal focusing (tF-GPc) to shape two-photon excitation for this purpose. the illumination patterns are generated automatically from fluorescence images of neurons and shaped to cover the cell body or dendrites, or distributed groups of cells. the tF-GPc two-photon excitation patterns generated large photocurrents in channelrhodopsin-2-expressing cultured cells and neurons and in mouse acute cortical slices. the amplitudes of the photocurrents can be precisely modulated by controlling the size and shape of the excitation volume and, thereby, be used to trigger single action potentials or trains of action potentials.

Url:

https://hal.archives-ouvertes.fr/hal-01963417

DOI: 10.1038/nmeth.1505

Affiliations:

Danemark, France, États-Unis

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000139
to stream Hal, to step Curation: 000139
to stream Hal, to step Checkpoint: 000138
to stream Main, to step Merge: 002428
to stream Main, to step Curation: 002397
to stream Main, to step Exploration: 002397
to stream France, to step Extraction: 000214

Links to Exploration step

Hal:hal-01963417

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Scanless two-photon excitation of channelrhodopsin-2</title>
<author><name sortKey="Papagiakoumou, Eirini" sort="Papagiakoumou, Eirini" uniqKey="Papagiakoumou E" first="Eirini" last="Papagiakoumou">Eirini Papagiakoumou</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-419825" status="OLD">          <idno type="IdRef">193594781</idno>
          <idno type="RNSR">201420754C</idno>
          <orgName>Laboratoire de Neurophotonique</orgName>
          <orgName type="acronym">UMR 8250</orgName>
          <date type="start">2014-01-01</date>
          <date type="end">2018-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences Fondamentales et Biomédicales45, rue des Saints Pères 75270 Paris Cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://neurophotonics.parisdescartes.cnrs.fr/?lang=en</ref>
          </desc>
          <listRelation>            <relation name="UMR 8250" active="#struct-301664" type="direct"></relation>
            <relation name="UMR 8250" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR 8250" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 8250" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Anselmi, Francesca" sort="Anselmi, Francesca" uniqKey="Anselmi F" first="Francesca" last="Anselmi">Francesca Anselmi</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-1629" status="OLD">          <orgName>Neurophysiologie et nouvelles microscopies</orgName>
          <orgName type="acronym">NNM (UM 82)</orgName>
          <date type="start">2010</date>
          <date type="end">2013</date>
          <desc>            <address>              <addrLine>45, rue des Saints Pères 75007 PARIS</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.physio.espci.fr</ref>
          </desc>
          <listRelation>            <relation name="UM 82" active="#struct-301664" type="direct"></relation>
            <relation name="U603" active="#struct-303623" type="direct"></relation>
            <relation name="UMR 1022" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UM 82" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="U603" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID">          <idno type="IdRef">026388278</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>            <address>              <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 1022" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Begue, Aurelien" sort="Begue, Aurelien" uniqKey="Begue A" first="Aurélien" last="Bègue">Aurélien Bègue</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-1629" status="OLD">          <orgName>Neurophysiologie et nouvelles microscopies</orgName>
          <orgName type="acronym">NNM (UM 82)</orgName>
          <date type="start">2010</date>
          <date type="end">2013</date>
          <desc>            <address>              <addrLine>45, rue des Saints Pères 75007 PARIS</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.physio.espci.fr</ref>
          </desc>
          <listRelation>            <relation name="UM 82" active="#struct-301664" type="direct"></relation>
            <relation name="U603" active="#struct-303623" type="direct"></relation>
            <relation name="UMR 1022" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UM 82" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="U603" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID">          <idno type="IdRef">026388278</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>            <address>              <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 1022" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="De Sars, Vincent" sort="De Sars, Vincent" uniqKey="De Sars V" first="Vincent" last="De Sars">Vincent De Sars</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-419825" status="OLD">          <idno type="IdRef">193594781</idno>
          <idno type="RNSR">201420754C</idno>
          <orgName>Laboratoire de Neurophotonique</orgName>
          <orgName type="acronym">UMR 8250</orgName>
          <date type="start">2014-01-01</date>
          <date type="end">2018-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences Fondamentales et Biomédicales45, rue des Saints Pères 75270 Paris Cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://neurophotonics.parisdescartes.cnrs.fr/?lang=en</ref>
          </desc>
          <listRelation>            <relation name="UMR 8250" active="#struct-301664" type="direct"></relation>
            <relation name="UMR 8250" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR 8250" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 8250" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Gluckstad, Jesper" sort="Gluckstad, Jesper" uniqKey="Gluckstad J" first="Jesper" last="Glückstad">Jesper Glückstad</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-50787" status="VALID">          <orgName>Technical University of Denmark [Lyngby]</orgName>
          <orgName type="acronym">DTU</orgName>
          <desc>            <address>              <addrLine>Anker Engelunds Vej 1,  Building 101A,  2800 Kgs. Lyngby</addrLine>
              <country key="DK"></country>
            </address>
            <ref type="url">http://www.dtu.dk/english</ref>
          </desc>
        </hal:affiliation>
<country>Danemark</country>
</affiliation>
</author>
<author><name sortKey="Isacoff, Ehud" sort="Isacoff, Ehud" uniqKey="Isacoff E" first="Ehud" last="Isacoff">Ehud Isacoff</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-82005" status="VALID">          <orgName>Lawrence Berkeley National Laboratory [Berkeley]</orgName>
          <orgName type="acronym">LBNL</orgName>
          <desc>            <address>              <addrLine>1 Cyclotron Rd. MS 50A-1148, Berkeley, CA 94720</addrLine>
              <country key="US"></country>
            </address>
            <ref type="url">http://www.lbl.gov</ref>
          </desc>
        </hal:affiliation>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Emiliani, Valentina" sort="Emiliani, Valentina" uniqKey="Emiliani V" first="Valentina" last="Emiliani">Valentina Emiliani</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-419825" status="OLD">          <idno type="IdRef">193594781</idno>
          <idno type="RNSR">201420754C</idno>
          <orgName>Laboratoire de Neurophotonique</orgName>
          <orgName type="acronym">UMR 8250</orgName>
          <date type="start">2014-01-01</date>
          <date type="end">2018-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences Fondamentales et Biomédicales45, rue des Saints Pères 75270 Paris Cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://neurophotonics.parisdescartes.cnrs.fr/?lang=en</ref>
          </desc>
          <listRelation>            <relation name="UMR 8250" active="#struct-301664" type="direct"></relation>
            <relation name="UMR 8250" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR 8250" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 8250" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-01963417</idno>
<idno type="halId">hal-01963417</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-01963417</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-01963417</idno>
<idno type="doi">10.1038/nmeth.1505</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Hal/Corpus">000139</idno>
<idno type="wicri:Area/Hal/Curation">000139</idno>
<idno type="wicri:Area/Hal/Checkpoint">000138</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000138</idno>
<idno type="wicri:doubleKey">1548-7091:2010:Papagiakoumou E:scanless:two:photon</idno>
<idno type="wicri:Area/Main/Merge">002428</idno>
<idno type="wicri:Area/Main/Curation">002397</idno>
<idno type="wicri:Area/Main/Exploration">002397</idno>
<idno type="wicri:Area/France/Extraction">000214</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Scanless two-photon excitation of channelrhodopsin-2</title>
<author><name sortKey="Papagiakoumou, Eirini" sort="Papagiakoumou, Eirini" uniqKey="Papagiakoumou E" first="Eirini" last="Papagiakoumou">Eirini Papagiakoumou</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-419825" status="OLD">          <idno type="IdRef">193594781</idno>
          <idno type="RNSR">201420754C</idno>
          <orgName>Laboratoire de Neurophotonique</orgName>
          <orgName type="acronym">UMR 8250</orgName>
          <date type="start">2014-01-01</date>
          <date type="end">2018-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences Fondamentales et Biomédicales45, rue des Saints Pères 75270 Paris Cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://neurophotonics.parisdescartes.cnrs.fr/?lang=en</ref>
          </desc>
          <listRelation>            <relation name="UMR 8250" active="#struct-301664" type="direct"></relation>
            <relation name="UMR 8250" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR 8250" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 8250" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Anselmi, Francesca" sort="Anselmi, Francesca" uniqKey="Anselmi F" first="Francesca" last="Anselmi">Francesca Anselmi</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-1629" status="OLD">          <orgName>Neurophysiologie et nouvelles microscopies</orgName>
          <orgName type="acronym">NNM (UM 82)</orgName>
          <date type="start">2010</date>
          <date type="end">2013</date>
          <desc>            <address>              <addrLine>45, rue des Saints Pères 75007 PARIS</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.physio.espci.fr</ref>
          </desc>
          <listRelation>            <relation name="UM 82" active="#struct-301664" type="direct"></relation>
            <relation name="U603" active="#struct-303623" type="direct"></relation>
            <relation name="UMR 1022" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UM 82" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="U603" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID">          <idno type="IdRef">026388278</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>            <address>              <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 1022" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Begue, Aurelien" sort="Begue, Aurelien" uniqKey="Begue A" first="Aurélien" last="Bègue">Aurélien Bègue</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-1629" status="OLD">          <orgName>Neurophysiologie et nouvelles microscopies</orgName>
          <orgName type="acronym">NNM (UM 82)</orgName>
          <date type="start">2010</date>
          <date type="end">2013</date>
          <desc>            <address>              <addrLine>45, rue des Saints Pères 75007 PARIS</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.physio.espci.fr</ref>
          </desc>
          <listRelation>            <relation name="UM 82" active="#struct-301664" type="direct"></relation>
            <relation name="U603" active="#struct-303623" type="direct"></relation>
            <relation name="UMR 1022" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UM 82" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="U603" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID">          <idno type="IdRef">026388278</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>            <address>              <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 1022" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="De Sars, Vincent" sort="De Sars, Vincent" uniqKey="De Sars V" first="Vincent" last="De Sars">Vincent De Sars</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-419825" status="OLD">          <idno type="IdRef">193594781</idno>
          <idno type="RNSR">201420754C</idno>
          <orgName>Laboratoire de Neurophotonique</orgName>
          <orgName type="acronym">UMR 8250</orgName>
          <date type="start">2014-01-01</date>
          <date type="end">2018-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences Fondamentales et Biomédicales45, rue des Saints Pères 75270 Paris Cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://neurophotonics.parisdescartes.cnrs.fr/?lang=en</ref>
          </desc>
          <listRelation>            <relation name="UMR 8250" active="#struct-301664" type="direct"></relation>
            <relation name="UMR 8250" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR 8250" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 8250" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Gluckstad, Jesper" sort="Gluckstad, Jesper" uniqKey="Gluckstad J" first="Jesper" last="Glückstad">Jesper Glückstad</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-50787" status="VALID">          <orgName>Technical University of Denmark [Lyngby]</orgName>
          <orgName type="acronym">DTU</orgName>
          <desc>            <address>              <addrLine>Anker Engelunds Vej 1,  Building 101A,  2800 Kgs. Lyngby</addrLine>
              <country key="DK"></country>
            </address>
            <ref type="url">http://www.dtu.dk/english</ref>
          </desc>
        </hal:affiliation>
<country>Danemark</country>
</affiliation>
</author>
<author><name sortKey="Isacoff, Ehud" sort="Isacoff, Ehud" uniqKey="Isacoff E" first="Ehud" last="Isacoff">Ehud Isacoff</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-82005" status="VALID">          <orgName>Lawrence Berkeley National Laboratory [Berkeley]</orgName>
          <orgName type="acronym">LBNL</orgName>
          <desc>            <address>              <addrLine>1 Cyclotron Rd. MS 50A-1148, Berkeley, CA 94720</addrLine>
              <country key="US"></country>
            </address>
            <ref type="url">http://www.lbl.gov</ref>
          </desc>
        </hal:affiliation>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Emiliani, Valentina" sort="Emiliani, Valentina" uniqKey="Emiliani V" first="Valentina" last="Emiliani">Valentina Emiliani</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-419825" status="OLD">          <idno type="IdRef">193594781</idno>
          <idno type="RNSR">201420754C</idno>
          <orgName>Laboratoire de Neurophotonique</orgName>
          <orgName type="acronym">UMR 8250</orgName>
          <date type="start">2014-01-01</date>
          <date type="end">2018-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences Fondamentales et Biomédicales45, rue des Saints Pères 75270 Paris Cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://neurophotonics.parisdescartes.cnrs.fr/?lang=en</ref>
          </desc>
          <listRelation>            <relation name="UMR 8250" active="#struct-301664" type="direct"></relation>
            <relation name="UMR 8250" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR 8250" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD">          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR 8250" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1038/nmeth.1505</idno>
<series><title level="j">Nature Methods</title>
<idno type="ISSN">1548-7091</idno>
<imprint><date type="datePub">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>Light-gated ion channels and pumps have made it possible to probe intact neural circuits by manipulating the activity of groups of genetically similar neurons. What is needed now is a method for precisely aiming the stimulating light at single neuronal processes, neurons or groups of neurons. We developed a method that combines generalized phase contrast with temporal focusing (tF-GPc) to shape two-photon excitation for this purpose. the illumination patterns are generated automatically from fluorescence images of neurons and shaped to cover the cell body or dendrites, or distributed groups of cells. the tF-GPc two-photon excitation patterns generated large photocurrents in channelrhodopsin-2-expressing cultured cells and neurons and in mouse acute cortical slices. the amplitudes of the photocurrents can be precisely modulated by controlling the size and shape of the excitation volume and, thereby, be used to trigger single action potentials or trains of action potentials.</p>
            </div>
</front>
</TEI>
<affiliations><list><country><li>Danemark</li>
<li>France</li>
<li>États-Unis</li>
</country>
</list>
<tree><country name="France"><noRegion><name sortKey="Papagiakoumou, Eirini" sort="Papagiakoumou, Eirini" uniqKey="Papagiakoumou E" first="Eirini" last="Papagiakoumou">Eirini Papagiakoumou</name>
</noRegion>
<name sortKey="Anselmi, Francesca" sort="Anselmi, Francesca" uniqKey="Anselmi F" first="Francesca" last="Anselmi">Francesca Anselmi</name>
<name sortKey="Begue, Aurelien" sort="Begue, Aurelien" uniqKey="Begue A" first="Aurélien" last="Bègue">Aurélien Bègue</name>
<name sortKey="De Sars, Vincent" sort="De Sars, Vincent" uniqKey="De Sars V" first="Vincent" last="De Sars">Vincent De Sars</name>
<name sortKey="Emiliani, Valentina" sort="Emiliani, Valentina" uniqKey="Emiliani V" first="Valentina" last="Emiliani">Valentina Emiliani</name>
</country>
<country name="Danemark"><noRegion><name sortKey="Gluckstad, Jesper" sort="Gluckstad, Jesper" uniqKey="Gluckstad J" first="Jesper" last="Glückstad">Jesper Glückstad</name>
</noRegion>
</country>
<country name="États-Unis"><noRegion><name sortKey="Isacoff, Ehud" sort="Isacoff, Ehud" uniqKey="Isacoff E" first="Ehud" last="Isacoff">Ehud Isacoff</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/France/Analysis

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000214 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000214 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    France
   |étape=   Analysis
   |type=    RBID
   |clé=     Hal:hal-01963417
   |texte=   Scanless two-photon excitation of channelrhodopsin-2
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Scanless two-photon excitation of channelrhodopsin-2

Scanless two-photon excitation of channelrhodopsin-2

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri