Epidemiology and Genetic Variability of HHV-8/KSHV in Pygmy and Bantu Populations in Cameroon
Identifieur interne : 002897 ( Pmc/Curation ); précédent : 002896; suivant : 002898Epidemiology and Genetic Variability of HHV-8/KSHV in Pygmy and Bantu Populations in Cameroon
Auteurs : Edouard Betsem [France, Cameroun] ; Olivier Cassar [France] ; Philippe V. Afonso [France] ; Arnaud Fontanet [France] ; Alain Froment [France] ; Antoine Gessain [France]Source :
- PLoS Neglected Tropical Diseases [ 1935-2727 ] ; 2014.
Abstract
Kaposi's sarcoma associated herpesvirus (KSHV/HHV-8) is the causal agent of all forms of Kaposi sarcoma. Molecular epidemiology of the variable K1 region identified five major subtypes exhibiting a clear geographical clustering. The present study is designed to gain new insights into the KSHV epidemiology and genetic diversity in Cameroon.
Bantu and Pygmy populations from remote rural villages were studied. Antibodies directed against latent nuclear antigens (LANA) were detected by indirect immunofluorescence using BC3 cells. Peripheral blood cell DNAs were subjected to a nested PCR amplifying a 737 bp K1 gene fragment. Consensus sequences were phylogenetically analyzed. We studied 2,063 persons (967 females, 1,096 males, mean age 39 years), either Bantus (1,276) or Pygmies (787). The Bantu group was older (42 versus 35 years: P<10−4). KSHV anti-LANA seroprevalence was of 37.2% (768/2063), with a significant increase with age (P<10−4) but no difference according to sex. Seroprevalence, as well as the anti-LANA antibodies titres, were higher in Bantus (43.2%) than in Pygmies (27.6%) (P<10−4), independently of age. We generated 29 K1 sequences, comprising 24 Bantus and five Pygmies. These sequences belonged to A5 (24 cases) or B (five cases) subtypes. They exhibited neither geographical nor ethnic aggregation. A5 strains showed a wide genetic diversity while the B strains were more homogenous and belonged to the B1 subgroup.
These data demonstrate high KSHV seroprevalence in the two major populations living in Southern and Eastern Cameroon with presence of mostly genetically diverse A5 but also B K1 subtypes.
Url:
DOI: 10.1371/journal.pntd.0002851
PubMed: 24831295
PubMed Central: 4022623
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Afonso</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Paris</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>CNRS, UMR3569, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, UMR3569, Paris</wicri:regionArea></affiliation></author><author><name sortKey="Fontanet, Arnaud" sort="Fontanet, Arnaud" uniqKey="Fontanet A" first="Arnaud" last="Fontanet">Arnaud Fontanet</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Institut Pasteur, Unité de Recherche et d'Expertise Epidémiologie des Maladies Emergentes, Département Infection et Epidémiologie, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Unité de Recherche et d'Expertise Epidémiologie des Maladies Emergentes, Département Infection et Epidémiologie, Paris</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Conservatoire National des Arts et Métiers, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Conservatoire National des Arts et Métiers, Paris</wicri:regionArea></affiliation></author><author><name sortKey="Froment, Alain" sort="Froment, Alain" uniqKey="Froment A" first="Alain" last="Froment">Alain Froment</name><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Institut de Recherche pour le Développement, Musée de l'Homme, Place du Trocadéro, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut de Recherche pour le Développement, Musée de l'Homme, Place du Trocadéro, Paris</wicri:regionArea></affiliation></author><author><name sortKey="Gessain, Antoine" sort="Gessain, Antoine" uniqKey="Gessain A" first="Antoine" last="Gessain">Antoine Gessain</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Paris</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>CNRS, UMR3569, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS, UMR3569, Paris</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS Neglected Tropical Diseases</title><idno type="ISSN">1935-2727</idno><idno type="eISSN">1935-2735</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Kaposi's sarcoma associated herpesvirus (KSHV/HHV-8) is the causal agent of all forms of Kaposi sarcoma. Molecular epidemiology of the variable K1 region identified five major subtypes exhibiting a clear geographical clustering. The present study is designed to gain new insights into the KSHV epidemiology and genetic diversity in Cameroon.</p></sec><sec><title>Methodology/Principal Findings</title><p>Bantu and Pygmy populations from remote rural villages were studied. Antibodies directed against latent nuclear antigens (LANA) were detected by indirect immunofluorescence using BC3 cells. Peripheral blood cell DNAs were subjected to a nested PCR amplifying a 737 bp K1 gene fragment. Consensus sequences were phylogenetically analyzed. We studied 2,063 persons (967 females, 1,096 males, mean age 39 years), either Bantus (1,276) or Pygmies (787). The Bantu group was older (42 versus 35 years: P<10<sup>−4</sup>). KSHV anti-LANA seroprevalence was of 37.2% (768/2063), with a significant increase with age (P<10<sup>−4</sup>) but no difference according to sex. Seroprevalence, as well as the anti-LANA antibodies titres, were higher in Bantus (43.2%) than in Pygmies (27.6%) (P<10<sup>−4</sup>), independently of age. We generated 29 K1 sequences, comprising 24 Bantus and five Pygmies. These sequences belonged to A5 (24 cases) or B (five cases) subtypes. They exhibited neither geographical nor ethnic aggregation. A5 strains showed a wide genetic diversity while the B strains were more homogenous and belonged to the B1 subgroup.</p></sec><sec><title>Conclusion</title><p>These data demonstrate high KSHV seroprevalence in the two major populations living in Southern and Eastern Cameroon with presence of mostly genetically diverse A5 but also B K1 subtypes.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chang, Y" uniqKey="Chang Y">Y Chang</name></author><author><name sortKey="Cesarman, E" uniqKey="Cesarman E">E Cesarman</name></author><author><name sortKey="Pessin, Ms" uniqKey="Pessin M">MS Pessin</name></author><author><name sortKey="Lee, F" uniqKey="Lee F">F Lee</name></author><author><name sortKey="Culpepper, J" uniqKey="Culpepper J">J Culpepper</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boshoff, C" uniqKey="Boshoff C">C Boshoff</name></author><author><name sortKey="Schulz, Tf" uniqKey="Schulz 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS Negl Trop Dis</journal-id><journal-id journal-id-type="iso-abbrev">PLoS Negl Trop Dis</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosntds</journal-id><journal-title-group><journal-title>PLoS Neglected Tropical Diseases</journal-title></journal-title-group><issn pub-type="ppub">1935-2727</issn><issn pub-type="epub">1935-2735</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24831295</article-id><article-id pub-id-type="pmc">4022623</article-id><article-id pub-id-type="publisher-id">PNTD-D-13-01612</article-id><article-id pub-id-type="doi">10.1371/journal.pntd.0002851</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology and Life Sciences</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Virology</subject><subj-group><subject>Viral Disease Diagnosis</subject><subject>Viruses and Cancer</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine and Health Sciences</subject><subj-group><subject>Epidemiology</subject><subj-group><subject>Disease Surveillance</subject><subject>Infectious Disease Epidemiology</subject><subject>Molecular Epidemiology</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Epidemiology and Genetic Variability of HHV-8/KSHV in Pygmy and Bantu Populations in Cameroon</article-title><alt-title alt-title-type="running-head">HHV-8/KSHV in Pygmies and Bantus in Cameroon</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Betsem</surname><given-names>Edouard</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cassar</surname><given-names>Olivier</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Afonso</surname><given-names>Philippe V.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fontanet</surname><given-names>Arnaud</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Froment</surname><given-names>Alain</given-names></name><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gessain</surname><given-names>Antoine</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Paris, France</addr-line></aff><aff id="aff2"><label>2</label><addr-line>CNRS, UMR3569, Paris, France</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Faculty of Medicine and Biomedical Sciences, University of Yaounde 1, Yaounde, Cameroon</addr-line></aff><aff id="aff4"><label>4</label><addr-line>Institut Pasteur, Unité de Recherche et d'Expertise Epidémiologie des Maladies Emergentes, Département Infection et Epidémiologie, Paris, France</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Conservatoire National des Arts et Métiers, Paris, France</addr-line></aff><aff id="aff6"><label>6</label><addr-line>Institut de Recherche pour le Développement, Musée de l'Homme, Place du Trocadéro, Paris, France</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Kasper</surname><given-names>Matthew</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>United States Naval Medical Research Unit Six, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>antoine.gessain@pasteur.fr</email></corresp><fn fn-type="COI-statement"><p>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: EB OC AG. Performed the experiments: EB OC. Analyzed the data: EB OC PVA AFo. Contributed reagents/materials/analysis tools: EB AFr AFo. Wrote the paper: EB OC PVA AG.</p></fn></author-notes><pub-date pub-type="collection"><month>5</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>15</day><month>5</month><year>2014</year></pub-date><volume>8</volume><issue>5</issue><elocation-id>e2851</elocation-id><history><date date-type="received"><day>17</day><month>10</month><year>2013</year></date><date date-type="accepted"><day>27</day><month>3</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 Betsem et al</copyright-statement><copyright-year>2014</copyright-year><copyright-holder>Betsem et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><abstract><sec><title>Background</title><p>Kaposi's sarcoma associated herpesvirus (KSHV/HHV-8) is the causal agent of all forms of Kaposi sarcoma. Molecular epidemiology of the variable K1 region identified five major subtypes exhibiting a clear geographical clustering. The present study is designed to gain new insights into the KSHV epidemiology and genetic diversity in Cameroon.</p></sec><sec><title>Methodology/Principal Findings</title><p>Bantu and Pygmy populations from remote rural villages were studied. Antibodies directed against latent nuclear antigens (LANA) were detected by indirect immunofluorescence using BC3 cells. Peripheral blood cell DNAs were subjected to a nested PCR amplifying a 737 bp K1 gene fragment. Consensus sequences were phylogenetically analyzed. We studied 2,063 persons (967 females, 1,096 males, mean age 39 years), either Bantus (1,276) or Pygmies (787). The Bantu group was older (42 versus 35 years: P<10<sup>−4</sup>). KSHV anti-LANA seroprevalence was of 37.2% (768/2063), with a significant increase with age (P<10<sup>−4</sup>) but no difference according to sex. Seroprevalence, as well as the anti-LANA antibodies titres, were higher in Bantus (43.2%) than in Pygmies (27.6%) (P<10<sup>−4</sup>), independently of age. We generated 29 K1 sequences, comprising 24 Bantus and five Pygmies. These sequences belonged to A5 (24 cases) or B (five cases) subtypes. They exhibited neither geographical nor ethnic aggregation. A5 strains showed a wide genetic diversity while the B strains were more homogenous and belonged to the B1 subgroup.</p></sec><sec><title>Conclusion</title><p>These data demonstrate high KSHV seroprevalence in the two major populations living in Southern and Eastern Cameroon with presence of mostly genetically diverse A5 but also B K1 subtypes.</p></sec></abstract><abstract abstract-type="summary"><title>Author Summary</title><p>Kaposi's sarcoma associated herpesvirus (KSHV/HHV-8) is the causal agent of one of the most frequent skin tumors found endemically or epidemically associated to HIV in Central and Eastern Africa. This highly variable virus tends to cluster geographically according to specific major subtypes. Its prevalence is high in that area and increases with age. Despite its association to all forms of Kaposi sarcoma and high prevalence described in some low income populations in Cameroon, KSHV arouses limited interest, and only few focused previous studies have looked into prevalence and modes of transmission, especially in families. Extended molecular epidemiology is unknown both in healthy individuals and in Kaposi patients, which led to looking for new insights among Bantu and Pygmy populations from rural villages in three regions of Cameroon sharing a quite similar living environment but yet genetically, socially, and culturally different. The present study is designed to describe variations of molecular subtypes in each of these population groups regarding their geography in rural areas of southern, central, and eastern Cameroon.</p></abstract><funding-group><funding-statement>This study was financially supported by: The Institut Pasteur; and through the Investissement d'Avenir as part of a Laboratoire d'Excellence (LabEx) French research program: Integrative Biology of Emerging Infectious Diseases (IBEID). Fundings were also received from the Service de Coopération et de l'Action Culturelle (SCAC) Yaoundé, the Institut National du Cancer (INCa), and the Association Virus Cancer Prevention. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="9"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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