Prevalence, Incidence and Determinants of Herpes Simplex Virus Type 2 Infection among HIV-Seronegative Women at High-Risk of HIV Infection: A Prospective Study in Beira, Mozambique
Identifieur interne : 002A85 ( Pmc/Corpus ); précédent : 002A84; suivant : 002A86Prevalence, Incidence and Determinants of Herpes Simplex Virus Type 2 Infection among HIV-Seronegative Women at High-Risk of HIV Infection: A Prospective Study in Beira, Mozambique
Auteurs : Ivete Meque ; Karine Dubé ; Paul J. Feldblum ; Archie C. A. Clements ; Arlinda Zango ; Fidelina Cumbe ; Pai Lien Chen ; Josefo J. Ferro ; Janneke H. Van De WijgertSource :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
To estimate the prevalence, incidence and determinants of herpes simplex type 2 (HSV-2) infection, and associations between HSV-2 and incident HIV infection, among women at higher risk for HIV infection in Beira, Mozambique.
Between 2009 and 2012, 411 women aged 18–35 years at higher risk of HIV acquisition (defined as having had two or more sexual partners in the month prior to study enrollment) were enrolled and followed monthly for one year. At each study visit, they were counseled, interviewed, and tested for HSV-2 and HIV antibodies.
The HSV-2 prevalence at baseline was 60.6% (95% CI: 55.7% –65.4%). Increasing age (aOR = 2.94, 95% CI: 1.74–4.97,
Young women engaging in risky sexual behaviors in Beira had high prevalence and incidence of HSV-2 infection. Improved primary HSV-2 control strategies are urgently needed in Beira.
Url:
DOI: 10.1371/journal.pone.0089705
PubMed: 24586973
PubMed Central: 3933639
Links to Exploration step
PMC:3933639Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Prevalence, Incidence and Determinants of Herpes Simplex Virus Type 2 Infection among HIV-Seronegative Women at High-Risk of HIV Infection: A Prospective Study in Beira, Mozambique</title><author><name sortKey="Meque, Ivete" sort="Meque, Ivete" uniqKey="Meque I" first="Ivete" last="Meque">Ivete Meque</name><affiliation><nlm:aff id="aff1"><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></nlm:aff></affiliation></author><author><name sortKey="Dube, Karine" sort="Dube, Karine" uniqKey="Dube K" first="Karine" last="Dubé">Karine Dubé</name><affiliation><nlm:aff id="aff2"><addr-line>FHI 360, Clinical Sciences Unit, Durham, North Carolina, United States of America</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><addr-line>United States Military HIV Research Program (MHRP), Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. (HJF), Bethesda, Maryland, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Feldblum, Paul J" sort="Feldblum, Paul J" uniqKey="Feldblum P" first="Paul J." last="Feldblum">Paul J. Feldblum</name><affiliation><nlm:aff id="aff2"><addr-line>FHI 360, Clinical Sciences Unit, Durham, North Carolina, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Clements, Archie C A" sort="Clements, Archie C A" uniqKey="Clements A" first="Archie C. A." last="Clements">Archie C. A. Clements</name><affiliation><nlm:aff id="aff4"><addr-line>University of Queensland, Infectious Disease Epidemiology Unit, School of Population Health, Brisbane, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Zango, Arlinda" sort="Zango, Arlinda" uniqKey="Zango A" first="Arlinda" last="Zango">Arlinda Zango</name><affiliation><nlm:aff id="aff1"><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cumbe, Fidelina" sort="Cumbe, Fidelina" uniqKey="Cumbe F" first="Fidelina" last="Cumbe">Fidelina Cumbe</name><affiliation><nlm:aff id="aff1"><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chen, Pai Lien" sort="Chen, Pai Lien" uniqKey="Chen P" first="Pai Lien" last="Chen">Pai Lien Chen</name><affiliation><nlm:aff id="aff2"><addr-line>FHI 360, Clinical Sciences Unit, Durham, North Carolina, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ferro, Josefo J" sort="Ferro, Josefo J" uniqKey="Ferro J" first="Josefo J." last="Ferro">Josefo J. Ferro</name><affiliation><nlm:aff id="aff1"><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></nlm:aff></affiliation></author><author><name sortKey="Van De Wijgert, Janneke H" sort="Van De Wijgert, Janneke H" uniqKey="Van De Wijgert J" first="Janneke H." last="Van De Wijgert">Janneke H. Van De Wijgert</name><affiliation><nlm:aff id="aff5"><addr-line>Amsterdam Institute for Global Health and Development (AIGHD) and Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff6"><addr-line>University of Liverpool, Institute of Infection and Global Health, Liverpool, United Kingdom</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24586973</idno><idno type="pmc">3933639</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933639</idno><idno type="RBID">PMC:3933639</idno><idno type="doi">10.1371/journal.pone.0089705</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">002A85</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002A85</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Prevalence, Incidence and Determinants of Herpes Simplex Virus Type 2 Infection among HIV-Seronegative Women at High-Risk of HIV Infection: A Prospective Study in Beira, Mozambique</title><author><name sortKey="Meque, Ivete" sort="Meque, Ivete" uniqKey="Meque I" first="Ivete" last="Meque">Ivete Meque</name><affiliation><nlm:aff id="aff1"><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></nlm:aff></affiliation></author><author><name sortKey="Dube, Karine" sort="Dube, Karine" uniqKey="Dube K" first="Karine" last="Dubé">Karine Dubé</name><affiliation><nlm:aff id="aff2"><addr-line>FHI 360, Clinical Sciences Unit, Durham, North Carolina, United States of America</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><addr-line>United States Military HIV Research Program (MHRP), Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. (HJF), Bethesda, Maryland, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Feldblum, Paul J" sort="Feldblum, Paul J" uniqKey="Feldblum P" first="Paul J." last="Feldblum">Paul J. Feldblum</name><affiliation><nlm:aff id="aff2"><addr-line>FHI 360, Clinical Sciences Unit, Durham, North Carolina, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Clements, Archie C A" sort="Clements, Archie C A" uniqKey="Clements A" first="Archie C. A." last="Clements">Archie C. A. Clements</name><affiliation><nlm:aff id="aff4"><addr-line>University of Queensland, Infectious Disease Epidemiology Unit, School of Population Health, Brisbane, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Zango, Arlinda" sort="Zango, Arlinda" uniqKey="Zango A" first="Arlinda" last="Zango">Arlinda Zango</name><affiliation><nlm:aff id="aff1"><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cumbe, Fidelina" sort="Cumbe, Fidelina" uniqKey="Cumbe F" first="Fidelina" last="Cumbe">Fidelina Cumbe</name><affiliation><nlm:aff id="aff1"><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chen, Pai Lien" sort="Chen, Pai Lien" uniqKey="Chen P" first="Pai Lien" last="Chen">Pai Lien Chen</name><affiliation><nlm:aff id="aff2"><addr-line>FHI 360, Clinical Sciences Unit, Durham, North Carolina, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ferro, Josefo J" sort="Ferro, Josefo J" uniqKey="Ferro J" first="Josefo J." last="Ferro">Josefo J. Ferro</name><affiliation><nlm:aff id="aff1"><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></nlm:aff></affiliation></author><author><name sortKey="Van De Wijgert, Janneke H" sort="Van De Wijgert, Janneke H" uniqKey="Van De Wijgert J" first="Janneke H." last="Van De Wijgert">Janneke H. Van De Wijgert</name><affiliation><nlm:aff id="aff5"><addr-line>Amsterdam Institute for Global Health and Development (AIGHD) and Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff6"><addr-line>University of Liverpool, Institute of Infection and Global Health, Liverpool, United Kingdom</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Objectives</title><p>To estimate the prevalence, incidence and determinants of herpes simplex type 2 (HSV-2) infection, and associations between HSV-2 and incident HIV infection, among women at higher risk for HIV infection in Beira, Mozambique.</p></sec><sec><title>Methods</title><p>Between 2009 and 2012, 411 women aged 18–35 years at higher risk of HIV acquisition (defined as having had two or more sexual partners in the month prior to study enrollment) were enrolled and followed monthly for one year. At each study visit, they were counseled, interviewed, and tested for HSV-2 and HIV antibodies.</p></sec><sec><title>Results</title><p>The HSV-2 prevalence at baseline was 60.6% (95% CI: 55.7% –65.4%). Increasing age (aOR = 2.94, 95% CI: 1.74–4.97, <italic>P</italic><0.001 and aOR = 3.39, 95% CI: 1.58–7.29, <italic>P</italic> = 0.002 for age groups of 21–24 and 25–35 years old respectively), lower educational level (aOR = 1.81, 95% CI: 1.09–3.02, <italic>P</italic> = 0.022), working full time (aOR = 8.56, 95% CI: 1.01–72.53, <italic>P</italic> = 0.049) and having practiced oral sex (aOR = 3.02, 95% CI: 1.16–7.89, <italic>P</italic> = 0.024) were strongly associated with prevalent HSV-2 infection. Thirty one participants seroconverted for HSV-2 (20.5%; 95% CI: 14.4% –27.9%) and 22 for HIV during the study period. The frequency of vaginal sex with a casual partner using a condom in the last 7 days was independently associated with incident HSV-2 infection (aOR = 1.91, 95% CI: 1.05–3.47, <italic>P</italic> = 0.034). Positive HSV-2 serology at baseline was not significantly associated with risk of subsequent HIV seroconversion.</p></sec><sec><title>Conclusions</title><p>Young women engaging in risky sexual behaviors in Beira had high prevalence and incidence of HSV-2 infection. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24586973</article-id><article-id pub-id-type="pmc">3933639</article-id><article-id pub-id-type="publisher-id">PONE-D-13-38551</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0089705</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Population biology</subject><subj-group><subject>Epidemiology</subject><subj-group><subject>Infectious disease epidemiology</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject><subj-group><subject>Clinical research design</subject></subj-group><subj-group><subject>Epidemiology</subject><subj-group><subject>Infectious disease epidemiology</subject></subj-group></subj-group><subj-group><subject>Infectious diseases</subject><subj-group><subject>Sexually transmitted diseases</subject><subj-group><subject>Herpes simplex</subject></subj-group></subj-group><subj-group><subject>Viral diseases</subject><subj-group><subject>HIV</subject><subj-group><subject>HIV epidemiology</subject><subject>HIV prevention</subject></subj-group></subj-group><subj-group><subject>Herpes simplex</subject></subj-group></subj-group><subj-group><subject>Infectious disease control</subject></subj-group></subj-group><subj-group><subject>Public health</subject></subj-group></subj-group></article-categories><title-group><article-title>Prevalence, Incidence and Determinants of Herpes Simplex Virus Type 2 Infection among HIV-Seronegative Women at High-Risk of HIV Infection: A Prospective Study in Beira, Mozambique</article-title><alt-title alt-title-type="running-head">HSV-2 Infection among High Risk Women in Beira</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Meque</surname><given-names>Ivete</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Dubé</surname><given-names>Karine</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Feldblum</surname><given-names>Paul J.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Clements</surname><given-names>Archie C. A.</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zango</surname><given-names>Arlinda</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cumbe</surname><given-names>Fidelina</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Pai Lien</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ferro</surname><given-names>Josefo J.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>van de Wijgert</surname><given-names>Janneke H.</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Universidade Católica de Moçambique/Catholic University of Mozambique (UCM), Centro de Investigação de Doenças Infecciosas/Center for Infectious Disease Research (CIDI), Beira, Mozambique</addr-line></aff><aff id="aff2"><label>2</label><addr-line>FHI 360, Clinical Sciences Unit, Durham, North Carolina, United States of America</addr-line></aff><aff id="aff3"><label>3</label><addr-line>United States Military HIV Research Program (MHRP), Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. (HJF), Bethesda, Maryland, United States of America</addr-line></aff><aff id="aff4"><label>4</label><addr-line>University of Queensland, Infectious Disease Epidemiology Unit, School of Population Health, Brisbane, Australia</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Amsterdam Institute for Global Health and Development (AIGHD) and Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands</addr-line></aff><aff id="aff6"><label>6</label><addr-line>University of Liverpool, Institute of Infection and Global Health, Liverpool, United Kingdom</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Leib</surname><given-names>David A.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Geisel School of Medicine at Dartmouth, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>ive.meque@yahoo.com.br</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: PJF JHvW. Performed the experiments: IM KD PJF AZ FC JJF JHvW. Analyzed the data: IM ACAC PLC JHvW. Contributed reagents/materials/analysis tools: IM KD PJF PLC JHvW. Wrote the paper: IM KD PJF ACAC JHvW AZ FC JJF PLC.</p></fn></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>24</day><month>2</month><year>2014</year></pub-date><volume>9</volume><issue>2</issue><elocation-id>e89705</elocation-id><history><date date-type="received"><day>18</day><month>9</month><year>2013</year></date><date date-type="accepted"><day>22</day><month>1</month><year>2014</year></date></history><permissions><copyright-year>2014</copyright-year><copyright-holder>Meque et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><sec><title>Objectives</title><p>To estimate the prevalence, incidence and determinants of herpes simplex type 2 (HSV-2) infection, and associations between HSV-2 and incident HIV infection, among women at higher risk for HIV infection in Beira, Mozambique.</p></sec><sec><title>Methods</title><p>Between 2009 and 2012, 411 women aged 18–35 years at higher risk of HIV acquisition (defined as having had two or more sexual partners in the month prior to study enrollment) were enrolled and followed monthly for one year. At each study visit, they were counseled, interviewed, and tested for HSV-2 and HIV antibodies.</p></sec><sec><title>Results</title><p>The HSV-2 prevalence at baseline was 60.6% (95% CI: 55.7% –65.4%). Increasing age (aOR = 2.94, 95% CI: 1.74–4.97, <italic>P</italic><0.001 and aOR = 3.39, 95% CI: 1.58–7.29, <italic>P</italic> = 0.002 for age groups of 21–24 and 25–35 years old respectively), lower educational level (aOR = 1.81, 95% CI: 1.09–3.02, <italic>P</italic> = 0.022), working full time (aOR = 8.56, 95% CI: 1.01–72.53, <italic>P</italic> = 0.049) and having practiced oral sex (aOR = 3.02, 95% CI: 1.16–7.89, <italic>P</italic> = 0.024) were strongly associated with prevalent HSV-2 infection. Thirty one participants seroconverted for HSV-2 (20.5%; 95% CI: 14.4% –27.9%) and 22 for HIV during the study period. The frequency of vaginal sex with a casual partner using a condom in the last 7 days was independently associated with incident HSV-2 infection (aOR = 1.91, 95% CI: 1.05–3.47, <italic>P</italic> = 0.034). Positive HSV-2 serology at baseline was not significantly associated with risk of subsequent HIV seroconversion.</p></sec><sec><title>Conclusions</title><p>Young women engaging in risky sexual behaviors in Beira had high prevalence and incidence of HSV-2 infection. Improved primary HSV-2 control strategies are urgently needed in Beira.</p></sec></abstract><funding-group><funding-statement>This study was partly funded by the U.S. Agency for International Development (USAID), under Cooperative Agreement No. GPO-A-00-05-00022-00, the Contraceptive and Reproductive Health Technologies Research and Utilization (CRTU) Program, and Cooperative Agreement No. GHO-A-00-09-00016-00, the Preventive Technologies Agreement (PTA). The study also received funding from the European and Developing Countries Clinical Trials Partnership (EDCTP) through the AfrEVacc project. IM was partially supported by the Australian Government (AUSAID) through Australian Development Scholarship (ADS) program. ACAC is funded by a NHMRC Career Development Award (#631619). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The views expressed are those of the authors and should not be construed to represent the positions of the funders. The views expressed in this paper are those of the authors and should not be construed to represent the positions of EDCTP, USAID, the U.S. Army or the U.S. Department of Defense.</funding-statement></funding-group><counts><page-count count="10"></page-count></counts></article-meta></front><body><sec id="s1"><title>Introduction</title><p>Herpes simplex virus type-2 (HSV-2) infection is a chronic sexually transmitted infection (STI) and the main cause of genital ulcer disease (GUD) worldwide <xref rid="pone.0089705-World1" ref-type="bibr">[1]</xref>–<xref rid="pone.0089705-PazBailey1" ref-type="bibr">[3]</xref>. This infection constitutes a substantial public health problem in sub-Saharan Africa because it increases the risk of HIV acquisition two to four fold <xref rid="pone.0089705-Celum1" ref-type="bibr">[2]</xref>, <xref rid="pone.0089705-Freeman1" ref-type="bibr">[4]</xref>–<xref rid="pone.0089705-Wald1" ref-type="bibr">[6]</xref>. HSV-2 and HIV have a synergistic relationship in that HSV-2 infection increases the susceptibility to and transmission of HIV, while HIV infection increases the susceptibility to HSV-2 infection and HSV-2 genital shedding <xref rid="pone.0089705-PazBailey1" ref-type="bibr">[3]</xref>, <xref rid="pone.0089705-Kapiga1" ref-type="bibr">[7]</xref>–<xref rid="pone.0089705-Tassiopoulos1" ref-type="bibr">[9]</xref>.</p><p>Reported risk factors for prevalent HSV-2 include older age <xref rid="pone.0089705-Kapiga1" ref-type="bibr">[7]</xref>, <xref rid="pone.0089705-Okuku1" ref-type="bibr">[8]</xref>, <xref rid="pone.0089705-Mugo1" ref-type="bibr">[10]</xref>, greater lifetime number of sexual partners <xref rid="pone.0089705-Mugo1" ref-type="bibr">[10]</xref>, <xref rid="pone.0089705-Arama1" ref-type="bibr">[11]</xref>, working in bars and hotels <xref rid="pone.0089705-Kapiga1" ref-type="bibr">[7]</xref>, <xref rid="pone.0089705-Chohan1" ref-type="bibr">[12]</xref>, prevalent HIV-1 infection, recent GUD or STI symptoms <xref rid="pone.0089705-Mugo1" ref-type="bibr">[10]</xref>, <xref rid="pone.0089705-Gottlieb1" ref-type="bibr">[13]</xref>, lower educational level <xref rid="pone.0089705-Arama1" ref-type="bibr">[11]</xref>, <xref rid="pone.0089705-WatsonJones1" ref-type="bibr">[14]</xref> and female gender <xref rid="pone.0089705-Mugo1" ref-type="bibr">[10]</xref>, <xref rid="pone.0089705-Gottlieb1" ref-type="bibr">[13]</xref>. HSV-2 prevalence and incidence are generally high among young African women, particularly those who engage in risky sexual behaviors, including exchanging sex for money or goods <xref rid="pone.0089705-Tassiopoulos1" ref-type="bibr">[9]</xref>, <xref rid="pone.0089705-Chohan1" ref-type="bibr">[12]</xref>, <xref rid="pone.0089705-WatsonJones1" ref-type="bibr">[14]</xref>, <xref rid="pone.0089705-Ramjee1" ref-type="bibr">[15]</xref>.</p><p>Studies in Southern Africa have shown variation in the prevalence of HSV-2 across diverse populations: 39.8%, 42.2% and 49.1% among male factory workers, urban and pregnant women in Zimbabwe, respectively <xref rid="pone.0089705-McFarland1" ref-type="bibr">[16]</xref>–<xref rid="pone.0089705-Munjoma1" ref-type="bibr">[18]</xref>; 84% among female sex workers at truck stops in South Africa <xref rid="pone.0089705-Ramjee1" ref-type="bibr">[15]</xref>; and 28% and 54.4% among GUD patients and male farm workers in Zambia, respectively <xref rid="pone.0089705-Makasa1" ref-type="bibr">[19]</xref>, <xref rid="pone.0089705-Heffron1" ref-type="bibr">[20]</xref>. No population-based HSV-2 data are available for Mozambique.</p><p>Mozambique is a country severely affected by HIV/AIDS, with a 2009 national prevalence estimated at 11.5% among adults aged 15–49 years and 13.1% among women of the same age <xref rid="pone.0089705-Instituto1" ref-type="bibr">[21]</xref>. Sofala Province (of which Beira is the capital) had the highest HIV prevalence of 15.5% among adults aged 15–49 years and 17.8% among women <xref rid="pone.0089705-Instituto1" ref-type="bibr">[21]</xref>. Only 2% of women aged 15–49 years in Sofala province had two or more sexual partners in the last 12 months <xref rid="pone.0089705-Instituto1" ref-type="bibr">[21]</xref>. However, the HIV prevalence among this group was 32.6%, almost double the provincial rate among all women <xref rid="pone.0089705-Zango1" ref-type="bibr">[22]</xref>. According to the National Strategic HIV and AIDS Response Plan 2010–2014, multiple sexual partners along with low condom usage are the main drivers of HIV acquisition in Mozambique and contribute to approximately 24–29% of all HIV new infections in the country <xref rid="pone.0089705-Repblicade1" ref-type="bibr">[23]</xref>.</p><p>The main aim of our study was to estimate HIV prevalence and incidence among HIV-seronegative women reporting two or more sexual partners in the past month in Beira, Mozambique, in preparation for clinical trials of new HIV prevention interventions. HSV-2 prevalence and incidence were also assessed given the synergies between the two infections. Here, we focus on the HSV-2 results and the association between HSV-2 infection and HIV seroconversion in this study population.</p></sec><sec sec-type="materials|methods" id="s2"><title>Materials and Methods</title><sec id="s2a"><title>Ethics Statement</title><p>The study was approved by the National Ethics Committee (Comité Nacional de Bioética para a Saúde or CNBS) in Maputo, Mozambique, the Protection of Human Subjects Committee (PHSC) at FHI 360, Durham, NC, USA, and the Division of Human Subjects Protection of the Walter Reed Army Institute of Research (WRAIR), Washington, DC, USA. The current analysis was further approved by the School of Population Health Ethics Committee of the University of Queensland, Brisbane, Australia. Literate participants provided written informed consent for the cross-sectional survey and the prospective cohort study; illiterate participants provided a thumb print and brought a literate witness who signed the consent form on their behalf. Participants received approximately 5 USD reimbursement per scheduled study visit.</p></sec><sec id="s2b"><title>Study Design and Population</title><p>Between December 2009 and September 2012, a total of 1,018 women aged 18–35 years reporting at least two sexual partners in the past month participated in a cross-sectional survey in Beira, Mozambique <xref rid="pone.0089705-Zango1" ref-type="bibr">[22]</xref>. HIV-negative women were offered enrollment in a prospective cohort study and the first 411 eligible women who consented were enrolled. Additional eligibility criteria included Mozambican citizenship, unknown HIV status, no history of antiretroviral therapy or non-therapeutic injecting drug use, and not currently enrolled in another HIV-related research study. Detailed recruitment procedures have been described elsewhere <xref rid="pone.0089705-Zango1" ref-type="bibr">[22]</xref>. Briefly, women were recruited from the community by outreach workers in places considered to be of potential high risk of HIV sexual acquisition, such as bars, barracks, kiosks, nightclubs, formal and informal markets, long-distance truck driver parking and secondary schools in and around the municipality of Beira. For virtually all women, enrollment into the longitudinal study occurred within 30 days of the cross-sectional survey visit. The small number of women who returned more than 30 days after their cross-sectional survey visit was required to repeat the survey procedures.</p></sec><sec id="s2c"><title>Study Procedures</title><p>Participants were followed up monthly over a 12-month period. At baseline and at each monthly follow-up visit, trained nurses conducted a face-to-face interview to obtain information about demographics, socio-economic status, sexual and contraceptive behavior and presence of STI symptoms. Blood samples were collected at each study visit for HIV and HSV-2 testing. HIV testing was performed at each study visit while the participant was at the study clinic using a rapid HIV test algorithm whereas HSV-2 testing was done in batches on baseline samples and on study exit samples (final study visit or HIV seroconversion visit) if the baseline sample was negative. Blood samples from participants who seroconverted for HIV were also tested by CD4 cytometry and HIV-1 RNA PCR. A urine pregnancy test was performed at every study visit. All participants received pre- and post-HIV test and safer sex counseling as well as male and female condoms free of charge. Participants who reported STI symptoms were given syndromic treatment according to the Mozambican Ministry of Health guidelines and pregnant women were referred to antenatal care <xref rid="pone.0089705-Repblicade2" ref-type="bibr">[24]</xref>.</p></sec><sec id="s2d"><title>Laboratory Methods</title><p>HSV-2 serological testing was performed at the UCM-CIDI Laboratory in Beira using the HerpeSelect 2 ELISA IgG assay (Focus Diagnostics, Cypress, CA, USA) following the manufacturer’s instructions. HIV rapid testing was performed in the research center in the presence of the study participant using the Determine HIV1/2 rapid test (Alere Medical Co. Ltd., Chiba, Japan) as a screening test and Uni-Gold (Trinity Biotech PLC, Bray, Ireland) as a confirmatory test. The SD Bioline HIV-1/2 3.0 rapid test (Standard Diagnostics Inc., Kyonggi-do, Korea) and/or ELISA were used to resolve discrepant rapid test results. Participants who tested HIV-positive received a CD4 count from the study clinic and were referred to public health centers to access HIV care. CD4 counts were determined on an EDTA blood sample at the clinical laboratory of the “Centro de Saúde Urbano Ponta-Gêa” using a BD FACSCalibur flow cytometry platform. Pregnancy testing was done by rapid urine hCG pregnancy test (Healthease Preg n Care, NEOMED IPA, Tzaneen, South Africa).</p></sec><sec id="s2e"><title>Statistical Analysis</title><p>All data were recorded on standardized case report forms that were double-entered into a database. Data were analyzed using STATA software version 11.2 (Statacorp, College Station, TX, USA).</p><p>Categorical variables were expressed as percentages, and continuous variables as medians with inter-quartile ranges (IQR). Baseline HSV-2 prevalence was calculated as the number of participants who tested positive for HSV-2 at cohort enrollment divided by the number of enrolled participants. HSV-2 incidence was calculated as the number of women who seroconverted for HSV-2 during follow-up divided by the total number of HSV-2 seronegative women at cohort enrollment <xref rid="pone.0089705-Webb1" ref-type="bibr">[26]</xref>.</p><p>Bivariable logistic regression models were used to assess determinants of prevalent baseline HSV-2 infection one at a time in two subgroups: demographic/socioeconomic factors and sexual behavioral factors. All factors associated with HSV-2 infection at p≤0.20 in the bivariable analyses were included in explanatory multivariable logistic regression models. The first multivariable model included demographic/socioeconomic factors only (Model I) and the second model sexual behavior factors only (with age forced into the model due to its importance as a confounder: Model II). Finally, variables with p≤0.20 in Models I and II were combined into a final multivariable model (Model III). The same modeling approach was used to assess determinants of HSV-2 incidence (comparing women who seroconverted for HSV-2 to those who remained negative, and excluding those who tested HSV-2 positive at baseline) with HSV-2 seroconversion as the outcome.</p><p>A survival analysis using Kaplan-Meier curves and the log-rank test was used to compare the HIV seroconversion curves of women who were HSV-2 seropositive and seronegative at baseline. Bivariable analysis with Cox proportional hazard models was used to calculate hazard ratios (HR) and 95% confidence intervals (CI) for baseline HSV-2 status and time-varying HSV-2 status during follow-up as a determinant of HIV acquisition. The proportional hazard assumption was tested and was valid. The two models (one for baseline HSV-2 status and one for time-varying HSV-2 status) had similar goodness of fit based on martingale residuals.</p></sec></sec><sec id="s3"><title>Results</title><sec id="s3a"><title>Characteristics of the Study Population</title><p>The first 411 HIV-negative cross-sectional survey participants who consented were enrolled in the prospective cohort study. Two women were excluded from all analyses: one woman had missing data at baseline and another did not meet the inclusion criterion of two or more sexual partners in the last month (<xref ref-type="fig" rid="pone-0089705-g001">Figure 1</xref>). The overall retention rate in the cohort study was 80%.</p><fig id="pone-0089705-g001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0089705.g001</object-id><label>Figure 1</label><caption><title>Participant flow diagram describing numbers enrolled, numbers tested for HSV-2 prevalence, and numbers included for HSV-2 incidence.</title></caption><graphic xlink:href="pone.0089705.g001"></graphic></fig><p>The median age of the participants was 21.0 years (IQR 19–24 years). About half of the participants (50.5%) were single and 56.0% had completed secondary school (grade 6–9). The majority of women (72.6%) were unemployed and 66.2% had no income in the last month. Forty three percent of women had been pregnant at least twice and 65.5% did not use a method of family planning. One third of the participants (33.3%) had sex for the first time at age 15 years or younger. More than two-thirds of women (70.4%) reported two sexual partners in the last month with the remaining 29.6% reporting three or more sexual partners. The majority of women (94.1%) reported to have a primary sexual partner, defined as a spouse, cohabitating partner, or a partner that the woman herself considered to be her most important partner. All other partners, including commercial partners, were defined as casual partners. About two-thirds of women (63.1%) reported that their primary partners had had sex with others in the last 6 months. Condom use was very low in this study population, with 79.9% and 57.0% of women reporting not having used condoms in the last month with a primary or casual partner, respectively. Oral and anal sex were relatively uncommon in this population (reported by 7.4% and 3.9% of women, respectively). The majority of the participants reported never having exchanged sex for money or goods (85.8%) or having been forced to have sex against their will (89.0%).</p></sec><sec id="s3b"><title>Baseline HSV-2 Seroprevalence and Determinants</title><p>The HSV-2 seroprevalence at baseline was 60.6% (95% CI = 55.7–65.4%). In bivariable logistic regression analysis, HSV-2 seroprevalence increased significantly with age, age at first marriage, lower level of education, working full-time, having a higher income, having had two or more lifetime pregnancies and currently using hormonal contraceptives (<xref ref-type="table" rid="pone-0089705-t001">Table 1</xref>). HSV-2 prevalence was also significantly higher among those whose primary partner was 30 years or older and those who did not use a condom during vaginal sex with a primary partner during the last month (<xref ref-type="table" rid="pone-0089705-t001">Table 1</xref>). Prevalent HSV-2 infection was significantly lower in those who had sex for the first time at age 16–17 compared to those who were older.</p><table-wrap id="pone-0089705-t001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0089705.t001</object-id><label>Table 1</label><caption><title>Determinants of baseline HSV-2 prevalence among 409 women, Beira, Mozambique.</title></caption><alternatives><graphic id="pone-0089705-t001-1" xlink:href="pone.0089705.t001"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td align="left" rowspan="1" colspan="1">Variable<sup>a</sup></td><td align="left" rowspan="1" colspan="1"><italic>n</italic></td><td align="left" rowspan="1" colspan="1"># HSV-2 positive/</td><td align="left" rowspan="1" colspan="1">Unadjusted OR</td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">(Prevalence, %)</td><td align="left" rowspan="1" colspan="1">95% CI</td><td align="left" rowspan="1" colspan="1"><italic>p</italic></td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Total</td><td align="left" rowspan="1" colspan="1">409<xref ref-type="table-fn" rid="nt107">b</xref></td><td align="left" rowspan="1" colspan="1">248 (60.6)</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Age group (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">18–20</td><td align="left" rowspan="1" colspan="1">179</td><td align="left" rowspan="1" colspan="1">78 (43.6)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">21–24</td><td align="left" rowspan="1" colspan="1">145</td><td align="left" rowspan="1" colspan="1">102 (70.3)</td><td align="left" rowspan="1" colspan="1">3.07 (1.93–4.88)</td><td align="left" rowspan="1" colspan="1"><0.001</td></tr><tr><td align="left" rowspan="1" colspan="1">25–35</td><td align="left" rowspan="1" colspan="1">85</td><td align="left" rowspan="1" colspan="1">68 (80.0)</td><td align="left" rowspan="1" colspan="1">5.18 (2.82–9.51)</td><td align="left" rowspan="1" colspan="1"><0.001</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Age of first marriage (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Never married</td><td align="left" rowspan="1" colspan="1">205</td><td align="left" rowspan="1" colspan="1">106 (51.7)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">≤17</td><td align="left" rowspan="1" colspan="1">69</td><td align="left" rowspan="1" colspan="1">50 (72.5)</td><td align="left" rowspan="1" colspan="1">2.46 (1.36–4.46)</td><td align="left" rowspan="1" colspan="1">0.003</td></tr><tr><td align="left" rowspan="1" colspan="1">≥18</td><td align="left" rowspan="1" colspan="1">124</td><td align="left" rowspan="1" colspan="1">87 (70.2)</td><td align="left" rowspan="1" colspan="1">2.2 (1.37–3.52)</td><td align="left" rowspan="1" colspan="1">0.001</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Education</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">High school (grade 10–12)</td><td align="left" rowspan="1" colspan="1">132</td><td align="left" rowspan="1" colspan="1">67 (50.8)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Secondary school (grade 6–9)</td><td align="left" rowspan="1" colspan="1">229</td><td align="left" rowspan="1" colspan="1">145 (63.3)</td><td align="left" rowspan="1" colspan="1">1.67 (1.08–2.59)</td><td align="left" rowspan="1" colspan="1">0.020</td></tr><tr><td align="left" rowspan="1" colspan="1">Primary school (grade1–5) or none</td><td align="left" rowspan="1" colspan="1">48</td><td align="left" rowspan="1" colspan="1">36 (75.0)</td><td align="left" rowspan="1" colspan="1">2.91 (1.39–6.08)</td><td align="left" rowspan="1" colspan="1">0.005</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Employment status</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Unemployed</td><td align="left" rowspan="1" colspan="1">297</td><td align="left" rowspan="1" colspan="1">170 (57.2)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Employed part time</td><td align="left" rowspan="1" colspan="1">92</td><td align="left" rowspan="1" colspan="1">59(64.1)</td><td align="left" rowspan="1" colspan="1">1.33 (0.82–2.17)</td><td align="left" rowspan="1" colspan="1">0.241</td></tr><tr><td align="left" rowspan="1" colspan="1">Employed full time</td><td align="left" rowspan="1" colspan="1">20</td><td align="left" rowspan="1" colspan="1">19 (95.0)</td><td align="left" rowspan="1" colspan="1">14.19 (1.88–107.43)</td><td align="left" rowspan="1" colspan="1">0.010</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Income last month (Mt)</bold><xref ref-type="table-fn" rid="nt108">c</xref></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No income</td><td align="left" rowspan="1" colspan="1">268</td><td align="left" rowspan="1" colspan="1">152 (56.7)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">≥500</td><td align="left" rowspan="1" colspan="1">61</td><td align="left" rowspan="1" colspan="1">38 (62.3)</td><td align="left" rowspan="1" colspan="1">1.26 (0.71–2.23)</td><td align="left" rowspan="1" colspan="1">0.427</td></tr><tr><td align="left" rowspan="1" colspan="1">501–15,000</td><td align="left" rowspan="1" colspan="1">72</td><td align="left" rowspan="1" colspan="1">54 (75.0)</td><td align="left" rowspan="1" colspan="1">2.29 (1.27–4.11)</td><td align="left" rowspan="1" colspan="1">0.006</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Number of lifetime pregnancies</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">0</td><td align="left" rowspan="1" colspan="1">124</td><td align="left" rowspan="1" colspan="1">55 (44.4)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">1</td><td align="left" rowspan="1" colspan="1">108</td><td align="left" rowspan="1" colspan="1">58 (53.7)</td><td align="left" rowspan="1" colspan="1">1.46 (0.87–2.44)</td><td align="left" rowspan="1" colspan="1">0.156</td></tr><tr><td align="left" rowspan="1" colspan="1">≥2</td><td align="left" rowspan="1" colspan="1">177</td><td align="left" rowspan="1" colspan="1">135 (76.3)</td><td align="left" rowspan="1" colspan="1">4.03 (2.46–6.62)</td><td align="left" rowspan="1" colspan="1"><0.001</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Family planning use</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">None</td><td align="left" rowspan="1" colspan="1">268</td><td align="left" rowspan="1" colspan="1">152 (56.7)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Hormonal contraceptives</td><td align="left" rowspan="1" colspan="1">95</td><td align="left" rowspan="1" colspan="1">68 (71.6)</td><td align="left" rowspan="1" colspan="1">1.92 (1.16–3.19)</td><td align="left" rowspan="1" colspan="1">0.012</td></tr><tr><td align="left" rowspan="1" colspan="1">Other <xref ref-type="table-fn" rid="nt109">d</xref></td><td align="left" rowspan="1" colspan="1">46</td><td align="left" rowspan="1" colspan="1">28 (59.6)</td><td align="left" rowspan="1" colspan="1">1.19 (0.63–2.25)</td><td align="left" rowspan="1" colspan="1">0.599</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Age at first sexual intercourse (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">≥18</td><td align="left" rowspan="1" colspan="1">102</td><td align="left" rowspan="1" colspan="1">68(66.7)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">16–17</td><td align="left" rowspan="1" colspan="1">163</td><td align="left" rowspan="1" colspan="1">85(52.2</td><td align="left" rowspan="1" colspan="1">0.54 (0.33–0.91)</td><td align="left" rowspan="1" colspan="1">0.021</td></tr><tr><td align="left" rowspan="1" colspan="1">≤15</td><td align="left" rowspan="1" colspan="1">136</td><td align="left" rowspan="1" colspan="1">91(66.9)</td><td align="left" rowspan="1" colspan="1">1.01 (0.59–1.74)</td><td align="left" rowspan="1" colspan="1">0.968</td></tr><tr><td colspan="2" align="left" rowspan="1"><bold>Age of primary partner (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No PP</td><td align="left" rowspan="1" colspan="1">24</td><td align="left" rowspan="1" colspan="1">13 (54.2)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes, aged 29 and younger</td><td align="left" rowspan="1" colspan="1">295</td><td align="left" rowspan="1" colspan="1">167 (56.6)</td><td align="left" rowspan="1" colspan="1">1.10 (0.48–2.55)</td><td align="left" rowspan="1" colspan="1">0.816</td></tr><tr><td align="left" rowspan="1" colspan="1">Yes, aged 30 and older</td><td align="left" rowspan="1" colspan="1">77</td><td align="left" rowspan="1" colspan="1">62 (80.5)</td><td align="left" rowspan="1" colspan="1">3.50 (1.31–9.33)</td><td align="left" rowspan="1" colspan="1">0.012</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Ever exchanged sex</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1">350</td><td align="left" rowspan="1" colspan="1">206 (58.9)</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1">58</td><td align="left" rowspan="1" colspan="1">41 (70.7)</td><td align="left" rowspan="1" colspan="1">1.69 (0.92–3.08)</td><td align="left" rowspan="1" colspan="1">0.090</td></tr><tr><td align="left" rowspan="1" colspan="1">Forced to have sex in the last month</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1">363</td><td align="left" rowspan="1" colspan="1">224 (61.7)</td><td align="left" rowspan="1" colspan="1">Referent</td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1">42</td><td align="left" rowspan="1" colspan="1">21 (50.0)</td><td align="left" rowspan="1" colspan="1">0.62 (0.33–1.18)</td><td align="left" rowspan="1" colspan="1">0.144</td></tr><tr><td align="left" rowspan="1" colspan="1">PP had sex with others in the last 6 months</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1">111</td><td align="left" rowspan="1" colspan="1">61 (55.0)</td><td align="left" rowspan="1" colspan="1">Referent</td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1">258</td><td align="left" rowspan="1" colspan="1">168 (65.1)</td><td align="left" rowspan="1" colspan="1">1.53 (0.97–2.41)</td><td align="left" rowspan="1" colspan="1">0.066</td></tr><tr><td align="left" rowspan="1" colspan="1">Oral sex with PP in the last month</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1">378</td><td align="left" rowspan="1" colspan="1">224(59.3)</td><td align="left" rowspan="1" colspan="1">Referent</td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1">30</td><td align="left" rowspan="1" colspan="1">23(76.7)</td><td align="left" rowspan="1" colspan="1">2.26 (0.95–5.39)</td><td align="left" rowspan="1" colspan="1">0.067</td></tr><tr><td align="left" rowspan="1" colspan="1">Frequency of vaginal sex with PP using condom in the last month</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Any use</td><td align="left" rowspan="1" colspan="1">82</td><td align="left" rowspan="1" colspan="1">41 (50.0)</td><td align="left" rowspan="1" colspan="1">Referent</td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">None</td><td align="left" rowspan="1" colspan="1">326</td><td align="left" rowspan="1" colspan="1">206 (63.2)</td><td align="left" rowspan="1" colspan="1">1.72 (1.05–2.80)</td><td align="left" rowspan="1" colspan="1">0.030</td></tr><tr><td align="left" rowspan="1" colspan="1">Pain during intercourse</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1">322</td><td align="left" rowspan="1" colspan="1">203 (63.0)</td><td align="left" rowspan="1" colspan="1">Referent</td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1">88</td><td align="left" rowspan="1" colspan="1">46 (52.3)</td><td align="left" rowspan="1" colspan="1">0.64 (0.40–1.03)</td><td align="left" rowspan="1" colspan="1">0.068</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt101"><label></label><p>OR, odds ratio; CI, Confidence intervals; PP, primary partner; CP, casual partner;</p></fn><fn id="nt102"><label></label><p>HSV-2, Herpes simplex virus type-2.</p></fn><fn id="nt103"><label></label><p>The total number of missing answers is 74.</p></fn><fn id="nt104"><label></label><p>? The following variables were also assessed for their association with prevalent HSV-2 but were not significant at the p<0.2 level in bivariable models:</p></fn><fn id="nt105"><label></label><p>Categorical variables: number of sexual partners, number of new sexual partners, oral sex with CP last month, anal sex with PP last month, anal sex with CP last month and frequency of vaginal sex with CP using condom last month.</p></fn><fn id="nt106"><label></label><p>Continuous variables: frequency of vaginal sex with PP last month, frequency of vaginal sex with CP last month, frequency of vaginal sex with PP using condom last 7 days and frequency of vaginal sex with CP using condom last 7 days.</p></fn><fn id="nt107"><label>b</label><p>Totals for the categories are not always equal to 409 because of missing data.</p></fn><fn id="nt108"><label>c</label><p>1 USD = 29.70 Meticais (Mt) as per 29/03/2013 (Millennium bim Bank).</p></fn><fn id="nt109"><label>d</label><p>Other Condoms/Copper IUD/Traditional.</p></fn></table-wrap-foot></table-wrap><p>In the final multivariable model, age was most strongly associated with baseline HSV-2 prevalence (adjusted odds ratio (aOR) = 2.94, 95% CI: 1.74–4.97, <italic>P</italic><0.001 and aOR = 3.39, 95% CI: 1.58–7.29, <italic>P</italic> = 0.002 for age groups of 21–24 and 25–35 years old respectively). Other determinants of HSV-2 prevalence were having had secondary school (grade 6–9) compared to high school (grade 10–12) education (aOR = 1.81, 95% CI: 1.09–3.02, <italic>P</italic> = 0.022), working full-time (aOR = 8.56, 95% CI: 1.01–72.53, <italic>P</italic> = 0.049) and having practiced oral sex (aOR = 3.02, 95% CI: 1.16–7.89, P = 0.024) (Model III, <xref ref-type="table" rid="pone-0089705-t002">Table 2</xref>). Having a primary partner who had sex with others in the last 6 months was not significant (aOR = 1.67, 95% CI: 0.99–2.80, <italic>P</italic> = 0.053). Conversely, having been forced to have sex in the last month was associated with reduced HSV-2 prevalence (aOR = 0.31, 95% CI: 0.15–0.66, <italic>P</italic> = 0.003).</p><table-wrap id="pone-0089705-t002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0089705.t002</object-id><label>Table 2</label><caption><title>Multivariable models of factors associated with HSV-2 prevalence in high risk women in Beira, Mozambique.</title></caption><alternatives><graphic id="pone-0089705-t002-2" xlink:href="pone.0089705.t002"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td align="left" rowspan="1" colspan="1">Variable</td><td colspan="2" align="left" rowspan="1">MODEL I <italic>n = </italic>402</td><td colspan="2" align="left" rowspan="1">MODEL II <italic>n</italic> = 404</td><td colspan="2" align="left" rowspan="1">MODEL III <italic>n</italic> = 406</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Adjusted OR</td><td align="left" rowspan="1" colspan="1"><italic>P</italic></td><td align="left" rowspan="1" colspan="1">Adjusted OR</td><td align="left" rowspan="1" colspan="1"><italic>p</italic></td><td align="left" rowspan="1" colspan="1">Adjusted OR</td><td align="left" rowspan="1" colspan="1"><italic>p</italic></td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">95% CI</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">95% CI</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">95% CI</td><td align="left" rowspan="1" colspan="1"></td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><bold>Age group (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">18–20</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">21–24</td><td align="left" rowspan="1" colspan="1">2.68 (1.60–4.47)</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1">3.22 (1.91–5.44)</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1">2.94 (1.74–4.97)</td><td align="left" rowspan="1" colspan="1"><0.001</td></tr><tr><td align="left" rowspan="1" colspan="1">25–35</td><td align="left" rowspan="1" colspan="1">2.89 (1.36–6.13)</td><td align="left" rowspan="1" colspan="1">0.006</td><td align="left" rowspan="1" colspan="1">4.95 (2.22–11.02)</td><td align="left" rowspan="1" colspan="1"><0.001</td><td align="left" rowspan="1" colspan="1">3.39 (1.58–7.29)</td><td align="left" rowspan="1" colspan="1">0.002</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Age first marriage (yrs)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Never married</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">17 or less (≤17)</td><td align="left" rowspan="1" colspan="1">0.98 (0.46–2.07)</td><td align="left" rowspan="1" colspan="1">0.959</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">18 and older (≥18)</td><td align="left" rowspan="1" colspan="1">0.82 (0.44–1.52)</td><td align="left" rowspan="1" colspan="1">0.527</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Education</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Grade 10–12</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Grade 6–9</td><td align="left" rowspan="1" colspan="1">1.61 (0.97–2.66)</td><td align="left" rowspan="1" colspan="1">0.063</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.81 (1.09–3.02)</td><td align="left" rowspan="1" colspan="1">0.022</td></tr><tr><td align="left" rowspan="1" colspan="1">Grade1–5 or none</td><td align="left" rowspan="1" colspan="1">1.93 (0.83–4.49)</td><td align="left" rowspan="1" colspan="1">0.124</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">2.24 (0.94–5.36)</td><td align="left" rowspan="1" colspan="1">0.069</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Employment status</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Unemployed</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Employed part time</td><td align="left" rowspan="1" colspan="1">1.03 (0.55–1.93)</td><td align="left" rowspan="1" colspan="1">0.934</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">0.93 (0.53–1.61)</td><td align="left" rowspan="1" colspan="1">0.793</td></tr><tr><td align="left" rowspan="1" colspan="1">Employed full time</td><td align="left" rowspan="1" colspan="1">5.21 (0.63–43.27)</td><td align="left" rowspan="1" colspan="1">0.127</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">8.56 (1.01–72.53)</td><td align="left" rowspan="1" colspan="1">0.049</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Income last month (Mt)<sup>1</sup></bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">>500</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">≤500</td><td align="left" rowspan="1" colspan="1">0.81 (0.36–1.86)</td><td align="left" rowspan="1" colspan="1">0.625</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No income</td><td align="left" rowspan="1" colspan="1">0.87 (0.43–1.79)</td><td align="left" rowspan="1" colspan="1">0.708</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold># lifetime pregnancies</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">0</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">1</td><td align="left" rowspan="1" colspan="1">1.30 (0.73–2.33)</td><td align="left" rowspan="1" colspan="1">0.379</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.11 (0.62–1.97)</td><td align="left" rowspan="1" colspan="1">0.725</td></tr><tr><td align="left" rowspan="1" colspan="1">≥2</td><td align="left" rowspan="1" colspan="1">2.20 (1.09–4.45)</td><td align="left" rowspan="1" colspan="1">0.028</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.72 (0.91–3.26)</td><td align="left" rowspan="1" colspan="1">0.094</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Family planning use</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">None</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Hormonal contraceptives</td><td align="left" rowspan="1" colspan="1">1.18 (0.66–2.10)</td><td align="left" rowspan="1" colspan="1">0.579</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Other<xref ref-type="table-fn" rid="nt114">a</xref></td><td align="left" rowspan="1" colspan="1">1.28 (0.64–2.58)</td><td align="left" rowspan="1" colspan="1">0.489</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Age at first sex (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">≥18</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">16–17</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">0.77 (0.42–1.39)</td><td align="left" rowspan="1" colspan="1">0.383</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">≤15</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.48 (0.79–2.76)</td><td align="left" rowspan="1" colspan="1">0.222</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Don’t know</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">0.47 (0.10–2.29)</td><td align="left" rowspan="1" colspan="1">0.349</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Age of PP (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No PP</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes, <30</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">0.77 (0.27–2.18)</td><td align="left" rowspan="1" colspan="1">0.629</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes, ≥30</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">0.93 (0.27–3.22)</td><td align="left" rowspan="1" colspan="1">0.916</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Ever exchanged sex</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Never</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.60 (0.81–3.16)</td><td align="left" rowspan="1" colspan="1">0.177</td><td align="left" rowspan="1" colspan="1">1.66 (0.83–3.32)</td><td align="left" rowspan="1" colspan="1">0.154</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Forced to have sex in the last month</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">0.38 (0.18–0.81)</td><td align="left" rowspan="1" colspan="1">0.012</td><td align="left" rowspan="1" colspan="1">0.31 (0.15–0.66)</td><td align="left" rowspan="1" colspan="1">0.003</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>PP sex with others in the last 6 months</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.52 (0.92–2.51)</td><td align="left" rowspan="1" colspan="1">0.104</td><td align="left" rowspan="1" colspan="1">1.67 (0.99–2.80)</td><td align="left" rowspan="1" colspan="1">0.053</td></tr><tr><td align="left" rowspan="1" colspan="1">Not applicable, no PP</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">0.68 (0.28–1.64)</td><td align="left" rowspan="1" colspan="1">0.391</td><td align="left" rowspan="1" colspan="1">0.91 (0.40–2.08)</td><td align="left" rowspan="1" colspan="1">0.819</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Oral sex with PP</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">3.03 (1.17–7.80)</td><td align="left" rowspan="1" colspan="1">0.022</td><td align="left" rowspan="1" colspan="1">3.02 (1.16–7.89)</td><td align="left" rowspan="1" colspan="1">0.024</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Frequency vaginal sex with PP using condom in the last month</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Any use</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">None</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.61 (0.92–2.81)</td><td align="left" rowspan="1" colspan="1">0.094</td><td align="left" rowspan="1" colspan="1">1.47 (0.83–2.60)</td><td align="left" rowspan="1" colspan="1">0.189</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Pain during intercourse in the past 3 months</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">0.55 (0.32–0.95)</td><td align="left" rowspan="1" colspan="1">0.031</td><td align="left" rowspan="1" colspan="1">0.62 (0.36–1.06)</td><td align="left" rowspan="1" colspan="1">0.081</td></tr><tr><td align="left" rowspan="1" colspan="1">R<sup>2</sup></td><td colspan="2" align="left" rowspan="1">0.1203</td><td colspan="2" align="left" rowspan="1">0.1374</td><td colspan="2" align="left" rowspan="1">0.1586</td></tr><tr><td align="left" rowspan="1" colspan="1">AIC</td><td colspan="2" align="left" rowspan="1">507.182</td><td colspan="2" align="left" rowspan="1">501.9356</td><td colspan="2" align="left" rowspan="1">492.8155</td></tr><tr><td align="left" rowspan="1" colspan="1">BIC</td><td colspan="2" align="left" rowspan="1">575.1217</td><td colspan="2" align="left" rowspan="1">569.9597</td><td colspan="2" align="left" rowspan="1">560.9235</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt110"><label></label><p>R<sup>2</sup>, Coefficient of Determination; AIC, Akaike Information Criterion; BIC, Bayesian Information Criterion;</p></fn><fn id="nt111"><label></label><p>Model I refers to the multivariable analysis of demographic/socioeconomic factors only;</p></fn><fn id="nt112"><label></label><p>Model II refers to the multivariable analysis of sexual behavior factors only, adjusted for age;</p></fn><fn id="nt113"><label></label><p>Model III refers to the multivariable analysis of both subgroups using cut off of P<0.20 in Models I and II.</p></fn><fn id="nt114"><label>a</label><p>‘Other’ Condoms/copper IUD/Traditional methods.</p></fn></table-wrap-foot></table-wrap></sec><sec id="s3c"><title>HSV-2 Incidence and Determinants</title><p>Of 161 women who were HSV-2 negative at baseline, 151 women were tested for HSV-2 during the follow-up period. Thirty one of them (20.5%) seroconverted for HSV-2 during follow-up. In bivariable and multivariable analyses, HSV-2 acquisition during follow-up was significantly associated with frequency of vaginal sex with a casual partner using a condom in the last 7 days (OR = 1.86, 95% CI 1.03–3.38, P = 0.041; aOR = 1.91, 95% CI: 1.05–3.47, P = 0.034). For women who were 21–24 years compared to women who were 18–20 years, the aOR for HSV-2 incidence was 2.36 (95% CI: 0.98–5.71, P = 0.057). (Model III; <xref ref-type="table" rid="pone-0089705-t003">Table 3</xref>).</p><table-wrap id="pone-0089705-t003" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0089705.t003</object-id><label>Table 3</label><caption><title>Multivariable models of factors associated with HSV-2 incidence in high risk women in Beira, Mozambique.</title></caption><alternatives><graphic id="pone-0089705-t003-3" xlink:href="pone.0089705.t003"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td align="left" rowspan="1" colspan="1"></td><td colspan="2" align="left" rowspan="1">MODEL I <italic>n = </italic>146</td><td colspan="2" align="left" rowspan="1">MODEL II <italic>n = </italic>151</td><td colspan="2" align="left" rowspan="1">MODEL III <italic>n = </italic>151</td></tr><tr><td align="left" rowspan="1" colspan="1">Variable<xref ref-type="table-fn" rid="nt116">a</xref></td><td align="left" rowspan="1" colspan="1">aOR (95% CI)</td><td align="left" rowspan="1" colspan="1"><italic>p</italic></td><td align="left" rowspan="1" colspan="1">aOR (95% CI)</td><td align="left" rowspan="1" colspan="1"><italic>p</italic></td><td align="left" rowspan="1" colspan="1">aOR (95% CI)</td><td align="left" rowspan="1" colspan="1"><italic>P</italic></td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><bold>Age group (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">18–20</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">21–24</td><td align="left" rowspan="1" colspan="1">1.92 (0.73–5.06)</td><td align="left" rowspan="1" colspan="1">0.185</td><td align="left" rowspan="1" colspan="1">2.27 (0.92–5.58)</td><td align="left" rowspan="1" colspan="1">0.074</td><td align="left" rowspan="1" colspan="1">2.36 (0.98–5.71)</td><td align="left" rowspan="1" colspan="1">0.057</td></tr><tr><td align="left" rowspan="1" colspan="1">25–35</td><td align="left" rowspan="1" colspan="1">0.82 (0.17–3.94)</td><td align="left" rowspan="1" colspan="1">0.802</td><td align="left" rowspan="1" colspan="1">1.39 (0.34–5.67)</td><td align="left" rowspan="1" colspan="1">0.644</td><td align="left" rowspan="1" colspan="1">1.37 (0.34–5.55)</td><td align="left" rowspan="1" colspan="1">0.657</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Age of first marriage (years)</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Never married</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">17 or less(≤17)</td><td align="left" rowspan="1" colspan="1">0.40 (0.07–2.18)</td><td align="left" rowspan="1" colspan="1">0.290</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">18 and older (≥18)</td><td align="left" rowspan="1" colspan="1">1.53 (0.47–4.92)</td><td align="left" rowspan="1" colspan="1">0.477</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold># lifetime pregnancies</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">0</td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">1</td><td align="left" rowspan="1" colspan="1">0.70 (0.15–2.72)</td><td align="left" rowspan="1" colspan="1">0.606</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">≥2</td><td align="left" rowspan="1" colspan="1">0.51 (0.17–1.70)</td><td align="left" rowspan="1" colspan="1">0.271</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Frequency of vaginal sex with a</bold><bold>PP in the last month*</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.00 (0.95–1.06)</td><td align="left" rowspan="1" colspan="1">0.956</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Frequency of vaginal sex with a</bold><bold>CP using a condom in the</bold><bold>last 7 days*</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">1.82 (0.94–3.52)</td><td align="left" rowspan="1" colspan="1">0.075</td><td align="left" rowspan="1" colspan="1">1.91 (1.05–3.47)</td><td align="left" rowspan="1" colspan="1">0.034</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>Anal sex with CP last month</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">No</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"><bold>Referent</bold></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1">Yes</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">2.59 (0.26–24.35)</td><td align="left" rowspan="1" colspan="1">0.421</td><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1"></td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>R<sup>2</sup></bold></td><td colspan="2" align="left" rowspan="1">0.052</td><td colspan="2" align="left" rowspan="1">0.055</td><td colspan="2" align="left" rowspan="1">0.0503</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>AIC</bold></td><td colspan="2" align="left" rowspan="1">157.13</td><td colspan="2" align="left" rowspan="1">156.89</td><td colspan="2" align="left" rowspan="1">153.6</td></tr><tr><td align="left" rowspan="1" colspan="1"><bold>BIC</bold></td><td colspan="2" align="left" rowspan="1">178.01</td><td colspan="2" align="left" rowspan="1">174.99</td><td colspan="2" align="left" rowspan="1">165.67</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt115"><label></label><p>aOR, Adjusted Odds Ratio; *Continuous variables; R<sup>2</sup>, Coefficient of Determination; AIC, Akaike Information Criterion; BIC, Bayesian Information Criterion; PP, Primary Partner; CP, Casual Partner.</p></fn><fn id="nt116"><label>a</label><p>The following variables were also assessed for their association with incident HSV-2 but were not significant at the p<0.2 level in bivariable models:</p></fn><fn id="nt117"><label></label><p>Categorical variables: education, employment status, family planning use, age at first sexual intercourse (years), number of sexual partners in the last month, number of new sexual partners in the last month, age of PP (years), ever exchanged sex, forced to have sex in the last month, PP had sex with others last 6 months, oral sex with PP in the last month, anal sex with PP in the last month, anal sex with CP in the last month, frequency of vaginal sex with PP using condom in the last month and frequency of vaginal sex with CP using condom in the last month.</p></fn><fn id="nt118"><label></label><p>Continuous variables: frequency vaginal sex with CP using condom in the last 7 days, frequency of vaginal sex with PP in the last month and frequency of vaginal sex with CP in the last month.</p></fn></table-wrap-foot></table-wrap></sec><sec id="s3d"><title>Associations between HSV-2 Infection and HIV Seroconversion</title><p>Two hundred forty eight of 409 women were HSV-2 positive at baseline, of which 233 (94.0%) remained HIV-negative and 15 (6.1%) became infected with HIV during follow-up. Among the 22 women with HIV seroconversion during the 12-month follow-up period, 15 (68.2%) were HSV-2 positive at baseline, 2 (9.1%) seroconverted for HSV-2, and 5 (22.7%) remained uninfected during the follow-up period. <xref ref-type="fig" rid="pone-0089705-g002">Figure 2</xref> shows Kaplan-Meier curves for time to HIV seroconversion according to HSV-2 status at baseline. The logrank test did not reach statistical significance (<italic>P</italic> = 0.406). A bivariable Cox regression model between HSV-2 status at baseline and subsequent HIV seroconversion showed a HR of 1.40 (95% CI: 0.57–3.44, P = 0.461). The relationship between HSV-2 serostatus at baseline and HIV seroconversion was not investigated further by multivariable analysis due to non-significant findings in bivariable analyses.</p><fig id="pone-0089705-g002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0089705.g002</object-id><label>Figure 2</label><caption><title>Kaplan-Meier curves for time to HIV seroconversion according to HSV-2 status at baseline.</title></caption><graphic xlink:href="pone.0089705.g002"></graphic></fig><p>Of 31 women who seroconverted for HSV-2, 2 (6.5%) also seroconverted for HIV and 29 (93.6%) remained HIV negative during the follow-up period. A Cox regression model using time-varying HSV-2 as predictor was used to assess the associations between incident HSV-2 infection and incident HIV infection. The model showed a HR of 1.64 (95% CI: 0.61–4.46, <italic>P</italic> = 0.329).</p></sec></sec><sec id="s4"><title>Discussion</title><p>This is the first study to assess prevalence, incidence, and determinants of HSV-2 infection in young, urban women in Mozambique at high risk of HIV infection. Women in this study had a high HSV-2 prevalence (ranging from 43.9% to 80.0% depending on the age group) and 20.5% seroconverted over a 12-month period, which is similar to rates found among high-risk women in other sub-Saharan African countries <xref rid="pone.0089705-Kapiga1" ref-type="bibr">[7]</xref>, <xref rid="pone.0089705-Tassiopoulos1" ref-type="bibr">[9]</xref>, <xref rid="pone.0089705-Weiss1" ref-type="bibr">[27]</xref>, <xref rid="pone.0089705-Vandepitte1" ref-type="bibr">[28]</xref>. These data suggest that interventions aimed at primary prevention of HSV-2, such as promotion of safer sexual behaviors, in young Mozambican women are urgently needed.</p><p>Increasing age was significantly associated with an increased HSV-2 seroprevalence and HSV-2 incidence, which is consistent with results of other African studies <xref rid="pone.0089705-Kapiga1" ref-type="bibr">[7]</xref>, <xref rid="pone.0089705-WatsonJones1" ref-type="bibr">[14]</xref>, <xref rid="pone.0089705-Weiss1" ref-type="bibr">[27]</xref>, <xref rid="pone.0089705-Vandepitte1" ref-type="bibr">[28]</xref>. Findings of the present study also add to the evidence that lower education, having a full time job, and having a primary partner who had sex with others in the last 6 months increased the risk of HSV-2 infection, although the latter not significantly. Most of the women in this study were working in informal settings, particularly in sales, food service and manual labor in bars, barracks and markets, and working full-time in these settings might be associated with exposure to multiple sexual partners. Studies among bar and hotel workers showed that these women are vulnerable because they engage in risky sexual behaviors <xref rid="pone.0089705-Kapiga1" ref-type="bibr">[7]</xref>, <xref rid="pone.0089705-Tassiopoulos1" ref-type="bibr">[9]</xref>, <xref rid="pone.0089705-Mgalla1" ref-type="bibr">[29]</xref>.</p><p>Surprisingly, having been forced to have sex in the last month was associated with a lower risk of HSV-2 prevalent infection in this study population. The association between HSV-2 infection and being forced to have sex against one’s will could have been confounded by other factors such as age and other sexual behavioral factors; this finding should therefore be interpreted with caution.</p><p>Oral sex was associated with a 4-fold increased risk of baseline HSV-2 prevalence in this population. A study among high-risk (imprisoned) women in Iran found a significant association between anal and oral sex and a positive HSV-2 IgG test result, suggesting that HSV-2 can be transmitted through anal and oral sex <xref rid="pone.0089705-Asgari1" ref-type="bibr">[30]</xref>. Reporting oral sex might also be associated with engaging in exchanging sex for money or other high-risk sexual behaviors. Having practiced vaginal sex with a casual partner using a condom in the last 7 days was associated with an increase in risk of HSV-2 acquisition in our study population. This may suggest that there was inconsistent use of condoms among the participants and their casual partners or overreporting of condom use due to social desirability bias. Reporting of condom use might reflect sexual risk-taking behavior <xref rid="pone.0089705-Chohan1" ref-type="bibr">[12]</xref>.</p><p>Various studies have suggested positive associations between prevalent and incident HSV-2 infection with incident HIV infection <xref rid="pone.0089705-Serwadda1" ref-type="bibr">[5]</xref>, <xref rid="pone.0089705-McFarland1" ref-type="bibr">[16]</xref>, <xref rid="pone.0089705-delMarPujadesRodriguez1" ref-type="bibr">[31]</xref>. Our findings also show trends in that direction, but did not reach statistical significance. We believe that this is most likely due to limited statistical power (22 HIV seroconversions translates into 11% statistical power to detect an association between incident HSV-2 infection and incident HIV infection), but it is also possible that an association between HSV-2 and HIV infection does not exist in our study setting.</p><p>Other limitations of our study include the possibility of selection and social desirability biases, and a lack of generalizability to all women in Beira due to recruitment of high risk women with two or more sexual partners only. In addition, studies suggest that the HerpeSelect HSV-2 IgG assay has lower specificity in African than Western populations <xref rid="pone.0089705-Hogrefe1" ref-type="bibr">[25]</xref>, <xref rid="pone.0089705-VanDyck1" ref-type="bibr">[32]</xref>, <xref rid="pone.0089705-DelanyMoretlwe1" ref-type="bibr">[33]</xref>. The authors of these studies have hypothesized that this may be due to cross-reactivity with HSV-1 or differences in circulating HSV-2 strains <xref rid="pone.0089705-Hogrefe1" ref-type="bibr">[25]</xref>, <xref rid="pone.0089705-VanDyck1" ref-type="bibr">[32]</xref>, <xref rid="pone.0089705-DelanyMoretlwe1" ref-type="bibr">[33]</xref>. We did not confirm positive HerpeSelect HSV-2 results with another test to improve specificity, which may have resulted in some false-positive test results. The HSV-2 prevalence and incidence in our study may therefore have been overestimated.</p><p>In conclusion, this study confirms that HSV-2 prevalence and incidence among young women at risk for HIV in Beira, Mozambique, is high and is associated with increasing age and high risk sexual behaviors. In the absence of an HSV-2 vaccine, health campaigns among young people should promote condom use, reduction in numbers of sexual partners, and a delay of sexual debut. HSV-2 prevention and counseling should be given high priority on the Mozambican health agenda, and should be addressed along with HIV and other STIs as part of a comprehensive prevention package.</p></sec></body><back><ack><p>We thank the study participants and the study teams at UCM-CIDI, FHI 360, and the AIGHD for their participation in and work on this study. We also thank Dr. Merlin L. Robb at the U.S. Military HIV Research Program (MHRP)/Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. (HJF), the Serviços Distritais de Saúde, Mulher e Acção Social da Beira, Direcção Provincial de Saúde de Sofala and Município da Beira for their support.</p></ack><ref-list><title>References</title><ref id="pone.0089705-World1"><label>1</label><mixed-citation publication-type="other">World Health Organization (2001) Herpes simplex virus type 2 programmatic and research priorities in developing countries. World Health Organization. London. 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|texte= Prevalence, Incidence and Determinants of Herpes Simplex Virus Type 2 Infection among HIV-Seronegative Women at High-Risk of HIV Infection: A Prospective Study in Beira, Mozambique
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:24586973" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SidaSubSaharaV1
| This area was generated with Dilib version V0.6.32. |  |