Scope and Limits of an Anamnestic Questionnaire in a Control-Induced Low-Endemicity Helminthiasis Setting in South-Central Côte d’Ivoire
Identifieur interne : 002A33 ( Pmc/Corpus ); précédent : 002A32; suivant : 002A34Scope and Limits of an Anamnestic Questionnaire in a Control-Induced Low-Endemicity Helminthiasis Setting in South-Central Côte d’Ivoire
Auteurs : Thomas Fürst ; Mamadou Ouattara ; Kigbafori D. Silué ; Dje N. N Oran ; Lukas G. Adiossan ; Isaac I. Bogoch ; Yao N Uessan ; Siaka Koné ; Jürg Utzinger ; Eliézer K. N OranSource :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Schistosomiasis and soil-transmitted helminthiasis are two high-burden neglected tropical diseases. In highly endemic areas, control efforts emphasize preventive chemotherapy. However, as morbidity, infection, and transmission begin to decrease, more targeted treatment is likely to become more cost-effective, provided that comparatively cheap diagnostic methods with reasonable accuracy are available.
Adults were administered an anamnestic questionnaire in mid-2010 during a cross-sectional epidemiological survey in the Taabo health demographic surveillance system in south-central Côte d’Ivoire. Questions pertaining to risk factors and signs and symptoms for schistosomiasis and soil-transmitted helminthiasis were included. The individuals’ helminth infection status and their belonging to three different anthelmintic treatment groups were compared with the questionnaire results (i) to inform the local health authorities about the epidemiological and clinical footprint of locally prevailing helminthiases, and (ii) to explore the scope and limits of an anamnestic questionnaire as monitoring tool, which eventually could help guiding the control of neglected tropical diseases in control-induced low-endemicity settings.
Our study sample consisted of 195 adults (101 males, 94 females). We found prevalences of hookworm,
The generally low prevalence and intensity of helminth infection in this part of south-central Côte d’Ivoire indicates that recent control efforts have turned our study area into a low endemicity setting. Our anamnestic questionnaire had low sensitivity and specificity to identify infected individuals or treatment groups.
Url:
DOI: 10.1371/journal.pone.0064380
PubMed: 23755120
PubMed Central: 3670890
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PMC:3670890Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Scope and Limits of an Anamnestic Questionnaire in a Control-Induced Low-Endemicity Helminthiasis Setting in South-Central Côte d’Ivoire</title><author><name sortKey="Furst, Thomas" sort="Furst, Thomas" uniqKey="Furst T" first="Thomas" last="Fürst">Thomas Fürst</name><affiliation><nlm:aff id="aff1"><addr-line>Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><addr-line>University of Basel, Basel, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ouattara, Mamadou" sort="Ouattara, Mamadou" uniqKey="Ouattara M" first="Mamadou" last="Ouattara">Mamadou Ouattara</name><affiliation><nlm:aff id="aff3"><addr-line>Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Silue, Kigbafori D" sort="Silue, Kigbafori D" uniqKey="Silue K" first="Kigbafori D." last="Silué">Kigbafori D. Silué</name><affiliation><nlm:aff id="aff3"><addr-line>Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="N Oran, Dje N" sort="N Oran, Dje N" uniqKey="N Oran D" first="Dje N." last="N Oran">Dje N. N Oran</name><affiliation><nlm:aff id="aff5"><addr-line>Programme National de Lutte contre la Schistosomiase, les Géohelminthiases et la Filariose Lymphatique, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Adiossan, Lukas G" sort="Adiossan, Lukas G" uniqKey="Adiossan L" first="Lukas G." last="Adiossan">Lukas G. Adiossan</name><affiliation><nlm:aff id="aff6"><addr-line>Hôpital Général de Taabo, Taabo Cité, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Bogoch, Isaac I" sort="Bogoch, Isaac I" uniqKey="Bogoch I" first="Isaac I." last="Bogoch">Isaac I. Bogoch</name><affiliation><nlm:aff id="aff7"><addr-line>Divisions of Internal Medicine and Infectious Diseases, Toronto General Hospital, Toronto, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="N Uessan, Yao" sort="N Uessan, Yao" uniqKey="N Uessan Y" first="Yao" last="N Uessan">Yao N Uessan</name><affiliation><nlm:aff id="aff4"><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kone, Siaka" sort="Kone, Siaka" uniqKey="Kone S" first="Siaka" last="Koné">Siaka Koné</name><affiliation><nlm:aff id="aff4"><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Utzinger, Jurg" sort="Utzinger, Jurg" uniqKey="Utzinger J" first="Jürg" last="Utzinger">Jürg Utzinger</name><affiliation><nlm:aff id="aff1"><addr-line>Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><addr-line>University of Basel, Basel, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="N Oran, Eliezer K" sort="N Oran, Eliezer K" uniqKey="N Oran E" first="Eliézer K." last="N Oran">Eliézer K. N Oran</name><affiliation><nlm:aff id="aff3"><addr-line>Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23755120</idno><idno type="pmc">3670890</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3670890</idno><idno type="RBID">PMC:3670890</idno><idno type="doi">10.1371/journal.pone.0064380</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">002A33</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002A33</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Scope and Limits of an Anamnestic Questionnaire in a Control-Induced Low-Endemicity Helminthiasis Setting in South-Central Côte d’Ivoire</title><author><name sortKey="Furst, Thomas" sort="Furst, Thomas" uniqKey="Furst T" first="Thomas" last="Fürst">Thomas Fürst</name><affiliation><nlm:aff id="aff1"><addr-line>Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><addr-line>University of Basel, Basel, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ouattara, Mamadou" sort="Ouattara, Mamadou" uniqKey="Ouattara M" first="Mamadou" last="Ouattara">Mamadou Ouattara</name><affiliation><nlm:aff id="aff3"><addr-line>Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Silue, Kigbafori D" sort="Silue, Kigbafori D" uniqKey="Silue K" first="Kigbafori D." last="Silué">Kigbafori D. Silué</name><affiliation><nlm:aff id="aff3"><addr-line>Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="N Oran, Dje N" sort="N Oran, Dje N" uniqKey="N Oran D" first="Dje N." last="N Oran">Dje N. N Oran</name><affiliation><nlm:aff id="aff5"><addr-line>Programme National de Lutte contre la Schistosomiase, les Géohelminthiases et la Filariose Lymphatique, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Adiossan, Lukas G" sort="Adiossan, Lukas G" uniqKey="Adiossan L" first="Lukas G." last="Adiossan">Lukas G. Adiossan</name><affiliation><nlm:aff id="aff6"><addr-line>Hôpital Général de Taabo, Taabo Cité, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Bogoch, Isaac I" sort="Bogoch, Isaac I" uniqKey="Bogoch I" first="Isaac I." last="Bogoch">Isaac I. Bogoch</name><affiliation><nlm:aff id="aff7"><addr-line>Divisions of Internal Medicine and Infectious Diseases, Toronto General Hospital, Toronto, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="N Uessan, Yao" sort="N Uessan, Yao" uniqKey="N Uessan Y" first="Yao" last="N Uessan">Yao N Uessan</name><affiliation><nlm:aff id="aff4"><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kone, Siaka" sort="Kone, Siaka" uniqKey="Kone S" first="Siaka" last="Koné">Siaka Koné</name><affiliation><nlm:aff id="aff4"><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author><author><name sortKey="Utzinger, Jurg" sort="Utzinger, Jurg" uniqKey="Utzinger J" first="Jürg" last="Utzinger">Jürg Utzinger</name><affiliation><nlm:aff id="aff1"><addr-line>Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><addr-line>University of Basel, Basel, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="N Oran, Eliezer K" sort="N Oran, Eliezer K" uniqKey="N Oran E" first="Eliézer K." last="N Oran">Eliézer K. N Oran</name><affiliation><nlm:aff id="aff3"><addr-line>Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Schistosomiasis and soil-transmitted helminthiasis are two high-burden neglected tropical diseases. In highly endemic areas, control efforts emphasize preventive chemotherapy. However, as morbidity, infection, and transmission begin to decrease, more targeted treatment is likely to become more cost-effective, provided that comparatively cheap diagnostic methods with reasonable accuracy are available.</p></sec><sec><title>Methodology</title><p>Adults were administered an anamnestic questionnaire in mid-2010 during a cross-sectional epidemiological survey in the Taabo health demographic surveillance system in south-central Côte d’Ivoire. Questions pertaining to risk factors and signs and symptoms for schistosomiasis and soil-transmitted helminthiasis were included. The individuals’ helminth infection status and their belonging to three different anthelmintic treatment groups were compared with the questionnaire results (i) to inform the local health authorities about the epidemiological and clinical footprint of locally prevailing helminthiases, and (ii) to explore the scope and limits of an anamnestic questionnaire as monitoring tool, which eventually could help guiding the control of neglected tropical diseases in control-induced low-endemicity settings.</p></sec><sec><title>Principal Findings</title><p>Our study sample consisted of 195 adults (101 males, 94 females). We found prevalences of hookworm, <italic>Trichuris trichiura</italic>, <italic>Schistosoma haematobium</italic>, and <italic>Schistosoma mansoni</italic> of 39.0%, 2.7%, 2.1%, and 2.1%, respectively. No <italic>Ascaris lumbricoides</italic> infection was found. Helminth infection intensities were generally very low. Seven, 74 and 79 participants belonged to three different treatment groups. Multivariable logistic regression models revealed statistically significant (p<0.05) associations between some risk factors, signs, and symptoms, and the different helminth infections and treatment groups. However, the risk factors, signs, and symptoms showed weak diagnostic properties.</p></sec><sec><title>Conclusions/Significance</title><p>The generally low prevalence and intensity of helminth infection in this part of south-central Côte d’Ivoire indicates that recent control efforts have turned our study area into a low endemicity setting. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23755120</article-id><article-id pub-id-type="pmc">3670890</article-id><article-id pub-id-type="publisher-id">PONE-D-12-40414</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0064380</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject><subj-group><subject>Diagnostic Medicine</subject></subj-group><subj-group><subject>Epidemiology</subject><subj-group><subject>Clinical Epidemiology</subject><subject>Epidemiological Methods</subject><subject>Infectious Disease Epidemiology</subject><subject>Social Epidemiology</subject><subject>Survey Methods</subject></subj-group></subj-group><subj-group><subject>Infectious Diseases</subject><subj-group><subject>Neglected Tropical Diseases</subject><subj-group><subject>Schistosomiasis</subject><subject>Soil-Transmitted Helminths</subject></subj-group></subj-group><subj-group><subject>Infectious Disease Control</subject></subj-group></subj-group><subj-group><subject>Public Health</subject><subj-group><subject>Behavioral and Social Aspects of Health</subject><subject>Disease Ecology</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Scope and Limits of an Anamnestic Questionnaire in a Control-Induced Low-Endemicity Helminthiasis Setting in South-Central Côte d’Ivoire</article-title><alt-title alt-title-type="running-head">Anamnestic Questionnaires for Helminth Control</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Fürst</surname><given-names>Thomas</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ouattara</surname><given-names>Mamadou</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Silué</surname><given-names>Kigbafori D.</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>N’Goran</surname><given-names>Dje N.</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Adiossan</surname><given-names>Lukas G.</given-names></name><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bogoch</surname><given-names>Isaac I.</given-names></name><xref ref-type="aff" rid="aff7"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>N’Guessan</surname><given-names>Yao</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Koné</surname><given-names>Siaka</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Utzinger</surname><given-names>Jürg</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>N’Goran</surname><given-names>Eliézer K.</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland</addr-line></aff><aff id="aff2"><label>2</label><addr-line>University of Basel, Basel, Switzerland</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire</addr-line></aff><aff id="aff4"><label>4</label><addr-line>Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Côte d’Ivoire</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Programme National de Lutte contre la Schistosomiase, les Géohelminthiases et la Filariose Lymphatique, Abidjan, Côte d’Ivoire</addr-line></aff><aff id="aff6"><label>6</label><addr-line>Hôpital Général de Taabo, Taabo Cité, Côte d’Ivoire</addr-line></aff><aff id="aff7"><label>7</label><addr-line>Divisions of Internal Medicine and Infectious Diseases, Toronto General Hospital, Toronto, Canada</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Diemert</surname><given-names>David Joseph</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>The George Washington University Medical Center, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>juerg.utzinger@unibas.ch</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: TF MO KDS SK JU EKN. Performed the experiments: TF MO KDS DNN LA YN EKN. Analyzed the data: TF IIB. Contributed reagents/materials/analysis tools: TF MO KDS DNN LA YN SK JU EKN. Wrote the paper: TF MO KDS IIB JU EKN.</p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>3</day><month>6</month><year>2013</year></pub-date><volume>8</volume><issue>6</issue><elocation-id>e64380</elocation-id><history><date date-type="received"><day>18</day><month>12</month><year>2012</year></date><date date-type="accepted"><day>11</day><month>4</month><year>2013</year></date></history><permissions><copyright-year>2013</copyright-year><copyright-holder>Fürst et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><sec><title>Background</title><p>Schistosomiasis and soil-transmitted helminthiasis are two high-burden neglected tropical diseases. In highly endemic areas, control efforts emphasize preventive chemotherapy. However, as morbidity, infection, and transmission begin to decrease, more targeted treatment is likely to become more cost-effective, provided that comparatively cheap diagnostic methods with reasonable accuracy are available.</p></sec><sec><title>Methodology</title><p>Adults were administered an anamnestic questionnaire in mid-2010 during a cross-sectional epidemiological survey in the Taabo health demographic surveillance system in south-central Côte d’Ivoire. Questions pertaining to risk factors and signs and symptoms for schistosomiasis and soil-transmitted helminthiasis were included. The individuals’ helminth infection status and their belonging to three different anthelmintic treatment groups were compared with the questionnaire results (i) to inform the local health authorities about the epidemiological and clinical footprint of locally prevailing helminthiases, and (ii) to explore the scope and limits of an anamnestic questionnaire as monitoring tool, which eventually could help guiding the control of neglected tropical diseases in control-induced low-endemicity settings.</p></sec><sec><title>Principal Findings</title><p>Our study sample consisted of 195 adults (101 males, 94 females). We found prevalences of hookworm, <italic>Trichuris trichiura</italic>, <italic>Schistosoma haematobium</italic>, and <italic>Schistosoma mansoni</italic> of 39.0%, 2.7%, 2.1%, and 2.1%, respectively. No <italic>Ascaris lumbricoides</italic> infection was found. Helminth infection intensities were generally very low. Seven, 74 and 79 participants belonged to three different treatment groups. Multivariable logistic regression models revealed statistically significant (p<0.05) associations between some risk factors, signs, and symptoms, and the different helminth infections and treatment groups. However, the risk factors, signs, and symptoms showed weak diagnostic properties.</p></sec><sec><title>Conclusions/Significance</title><p>The generally low prevalence and intensity of helminth infection in this part of south-central Côte d’Ivoire indicates that recent control efforts have turned our study area into a low endemicity setting. Our anamnestic questionnaire had low sensitivity and specificity to identify infected individuals or treatment groups.</p></sec></abstract><funding-group><funding-statement>The authors thank Fairmed for sustained funding that allowed us to establish and run the Taabo health demographic surveillance system in south-central Côte d’Ivoire, and the accompanying health interventions with the aim to reduce the mortality and morbidity due to malaria and neglected tropical diseases. TF is associated to the National Centre of Competence in Research North-South and received financial support through a Pro*Doc Research Module from the Swiss National Science Foundation (SNSF; project no. PDFMP3-123185). Furthermore, he is thankful for a personal stipend from the Freiwillige Akademische Gesellschaft Basel. JU and EKN are grateful to the SNSF (project no. IZ70Z-123900). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="10"></page-count></counts></article-meta></front><body><sec id="s1"><title>Introduction</title><p>Schistosomiasis and soil-transmitted helminthiasis are two high-burden neglected tropical diseases <xref ref-type="bibr" rid="pone.0064380-WHO1">[1]</xref>. Burden estimates, as expressed in disability-adjusted life years (DALYs), range from 1.7 to 70 million DALYs for schistosomiasis <xref ref-type="bibr" rid="pone.0064380-WHO1">[1]</xref>–<xref ref-type="bibr" rid="pone.0064380-Murray1">[8]</xref>, and from 2.9 to 39 million DALYs for soil-transmitted helminthiasis <xref ref-type="bibr" rid="pone.0064380-WHO1">[1]</xref>, <xref ref-type="bibr" rid="pone.0064380-Lammie1">[3]</xref>, <xref ref-type="bibr" rid="pone.0064380-Brooker1">[4]</xref>, <xref ref-type="bibr" rid="pone.0064380-Murray1">[8]</xref>, <xref ref-type="bibr" rid="pone.0064380-Brooker2">[9]</xref>. The awareness for these high disease burdens have increased in the past few years, but despite the fact that control efforts are going to scale, a variety of high and low endemicity zones remain around the globe <xref ref-type="bibr" rid="pone.0064380-deSilva1">[10]</xref>, <xref ref-type="bibr" rid="pone.0064380-Gryseels1">[11]</xref>.</p><p>The main strategy to combat the diseases in highly endemic areas is morbidity control. Once morbidity has decreased, the control strategies foresee a progressive shift toward infection and transmission control, surveillance and case detection and, ultimately, local elimination <xref ref-type="bibr" rid="pone.0064380-Engels1">[12]</xref>–<xref ref-type="bibr" rid="pone.0064380-Rollinson1">[14]</xref>. Consequently, the diagnosis, treatment, and control strategies have to be adapted <xref ref-type="bibr" rid="pone.0064380-Bergquist1">[13]</xref>, <xref ref-type="bibr" rid="pone.0064380-Guyatt1">[15]</xref>, <xref ref-type="bibr" rid="pone.0064380-Brooker3">[16]</xref>. For instance, in high endemicity areas, preventive chemotherapy (i.e., regular treatment of high-risk groups without prior diagnosis <xref ref-type="bibr" rid="pone.0064380-Gabrielli1">[17]</xref>) using available, safe, and efficacious drugs that are inexpensive or donated by pharmaceutical companies, is the most widely used strategy <xref ref-type="bibr" rid="pone.0064380-Gabrielli1">[17]</xref>, <xref ref-type="bibr" rid="pone.0064380-WHO2">[18]</xref>. However, as morbidity, infection, and transmission begin to decrease, more targeted treatment might become more cost-effective <xref ref-type="bibr" rid="pone.0064380-Raso1">[19]</xref>, provided that comparatively cheap diagnostic methods with reasonable accuracy are available <xref ref-type="bibr" rid="pone.0064380-Bergquist1">[13]</xref>. Such diagnostics have to consider the changes in parasite ecology that increasingly occur due to the scale-up of preventive chemotherapy and the expansion of more integrated control strategies, which tackle multiple helminth species simultaneously <xref ref-type="bibr" rid="pone.0064380-WHO2">[18]</xref>, <xref ref-type="bibr" rid="pone.0064380-Hotez2">[20]</xref> and also on non-drug-based routes (e.g., providing clean water and improved sanitation) <xref ref-type="bibr" rid="pone.0064380-Ehrenberg1">[21]</xref>, <xref ref-type="bibr" rid="pone.0064380-Utzinger1">[22]</xref>. Hence, control-induced low-endemicity settings have become the new reality of helminth epidemiology in many areas.</p><p>Simple, rapid, inexpensive, and culturally adapted questionnaires have been considered as useful diagnostic tools to screen communities and guiding control interventions over the past years <xref ref-type="bibr" rid="pone.0064380-Lengeler1">[23]</xref>. Regarding schistosomiasis, for example, school-based questionnaires proved useful for identification of high-risk communities of <italic>Schistosoma haematobium</italic><xref ref-type="bibr" rid="pone.0064380-Lengeler2">[24]</xref>. Indeed, school prevalence of self-reported blood in urine correlates well with the prevalence of <italic>S. haematobium</italic><xref ref-type="bibr" rid="pone.0064380-Lengeler2">[24]</xref>. A simple anamnestic questionnaire, including questions on signs, symptoms, and water contact patterns, allowed individual diagnosis of <italic>S. japonicum</italic><xref ref-type="bibr" rid="pone.0064380-Tan1">[25]</xref>. However, adaptation for <italic>S. mansoni</italic> and other helminth infections proved to be more difficult <xref ref-type="bibr" rid="pone.0064380-Bergquist1">[13]</xref>, <xref ref-type="bibr" rid="pone.0064380-Lengeler2">[24]</xref>, <xref ref-type="bibr" rid="pone.0064380-Booth1">[26]</xref> and the usefulness of anamnestic questionnaires seems to be restrained in regions with naturally low helminth endemicity as noticeable signs and symptoms are rare. Further research is therefore needed to determine the scope and limits of anamnestic questionnaires when they are employed over the course of control programs, which are characterized by declining morbidity and prevalence rates <xref ref-type="bibr" rid="pone.0064380-Bergquist1">[13]</xref>, <xref ref-type="bibr" rid="pone.0064380-Lengeler1">[23]</xref>, <xref ref-type="bibr" rid="pone.0064380-Brooker4">[27]</xref>.</p><p>In this paper, we report our experience from a cross-sectional survey carried out in mid-2010 as part of a prospective longitudinal monitoring of people’s malaria and neglected tropical diseases status in the Taabo health demographic surveillance system (HDSS) in south-central Côte d’Ivoire. The study area represents an epidemiological situation, which is influenced by helminth control activities <xref ref-type="bibr" rid="pone.0064380-Righetti1">[28]</xref>. The two objectives of the survey were (i) to assess risk factors, signs, and symptoms related to schistosomiasis and soil-transmitted helminthiasis in order to inform the local health authorities about the epidemiological and clinical footprint of these two helminthiasis, and (ii) to explore the scope and limits of an anamnestic questionnaire as monitoring tool, which eventually could help guiding the control of neglected tropical diseases in control-induced low-endemicity settings.</p></sec><sec sec-type="methods" id="s2"><title>Methods</title><sec id="s2a"><title>Ethics Statement</title><p>The study protocol was cleared by the institutional research commissions of the Centre Suisse de Recherches Scientifiques en Côte d’Ivoire (CSRS; Abidjan, Côte d’Ivoire) and the Swiss Tropical and Public Health Institute (Swiss TPH; Basel, Switzerland). Ethical clearance was obtained from the Comité National d’Ethique et de la Recherche (CNER) in Côte d’Ivoire (reference no. 1086 MSHP/CNER) and the ethics committee in Basel (EKBB; reference no. 316/08).</p><p>The study was integrated in the second annual parasitological survey and preventive chemotherapy campaign in the Taabo HDSS in June 2010. District and village authorities and the general public were informed about the purpose, procedures, potential risks and benefits of the annual survey, treatment, and the current questionnaire study. Written informed consent was obtained from all participants of the present study. Everybody living in the area of the Taabo HDSS was invited for a free anthelmintic treatment with ivermectin (∼200 µg/kg using a dose pole) and albendazole (400 mg single oral dose), irrespective of the infection status or participation in the present study <xref ref-type="bibr" rid="pone.0064380-WHO3">[29]</xref>, <xref ref-type="bibr" rid="pone.0064380-WHO4">[30]</xref>. Praziquantel (40 mg/kg using a dose pole), was administered half a year later for individuals aged 5 years and above in the course of a preventive chemotherapy campaign against schistosomiasis <xref ref-type="bibr" rid="pone.0064380-WHO3">[29]</xref>. Medical staff accompanied the survey, anthelmintic treatment, and follow-up.</p></sec><sec id="s2b"><title>Study Area and Population</title><p>The study area and population have been described elsewhere <xref ref-type="bibr" rid="pone.0064380-Frst1">[31]</xref>. In brief, the Taabo HDSS was established in 2008 around Lake Taabo in south-central Côte d’Ivoire. It covers most of the rural sub-district of Taabo with a surface area of approximately 1,000 km<sup>2</sup>. Since 2012, the Taabo HDSS is a member center of the INDEPTH Network (see <ext-link ext-link-type="uri" xlink:href="http://www.indepth-network.org">http://www.indepth-network.org</ext-link>). The main station of the Taabo HDSS is located in Taabo Cité, the only small urban settlement in the Taabo HDSS, 160 km north-west of Abidjan. Most people in the region cultivate yams, manioc, and banana mainly for subsistence. Coffee and cacao are farmed as cash crops. Furthermore, there is a minority of fishermen, artisans, shopkeepers, and businessmen.</p><p>Lake Taabo is a man-made impoundment resulting from damming up the Bandama River in the late 1970s for hydroelectric power generation <xref ref-type="bibr" rid="pone.0064380-NGoran1">[32]</xref>. Hence, the study area underwent major ecologic and demographic transformation, which favored the spread of schistosomiasis <xref ref-type="bibr" rid="pone.0064380-NGoran1">[32]</xref>, <xref ref-type="bibr" rid="pone.0064380-NGoran2">[33]</xref> and might have influenced patterns of other helminth infections <xref ref-type="bibr" rid="pone.0064380-Glinz1">[34]</xref>, <xref ref-type="bibr" rid="pone.0064380-Becker1">[35]</xref> and malaria <xref ref-type="bibr" rid="pone.0064380-Silu1">[36]</xref>. Before the establishment of the Taabo HDSS, different studies provided sporadic anthelmintic treatment to some village communities. Therefore, the development of more systematic and integrated disease control measures, particularly annual preventive chemotherapy campaigns against helminthiases and a strengthening of the health system became specific objectives of the Taabo HDSS.</p></sec><sec id="s2c"><title>Data Collection</title><p>The parasitological data for the present study were obtained in the frame of the second cross-sectional survey and preventive chemotherapy campaign (carried out once every year) in the Taabo HDSS. While everybody living in the area of the Taabo HDSS was invited to participate in the preventive chemotherapy campaign, the members of approximately 7% of all registered households were selected for the epidemiological survey based on a stratified random sampling procedure. They were asked to provide fresh morning stool and urine samples. The samples were transferred to the laboratory of the general hospital in Taabo Cité and analyzed the same day by experienced laboratory technicians using standardized, quality-controlled techniques <xref ref-type="bibr" rid="pone.0064380-Katz1">[37]</xref>, <xref ref-type="bibr" rid="pone.0064380-WHO5">[38]</xref>. In short, duplicate Kato-Katz thick smears were prepared with 41.7 mg of stool and microscopically examined for <italic>S. mansoni</italic> and soil-transmitted helminths (<italic>Ascaris lumbricoides</italic>, <italic>Trichuris trichiura</italic>, and hookworm). In order to obtain infection intensities as expressed in eggs per gram of stool (EPG), the sum of the helminth-specific egg counts from the two Kato-Katz thick smears were multiplied by a factor 12 <xref ref-type="bibr" rid="pone.0064380-WHO3">[29]</xref>. Urine samples were vigorously shaken, 10 ml subjected to a filtration, and the filters, after adding a drop of iodine Lugol, microscopically examined for <italic>S. haematobium</italic>. Five percent of the Kato-Katz thick smears and the urine filters were re-examined by a senior technician. In case of disagreement, the slides were read a third time and the results discussed among the technicians until agreement was reached.</p><p>All heads of households and, if possible, a second adult household member of the opposite sex were eligible for the present study. On the day of the epidemiological survey, all eligible individuals were invited to complete a questionnaire on risk factors, signs, and symptoms pertaining to different neglected tropical diseases (<xref ref-type="supplementary-material" rid="pone.0064380.s001">Text S1</xref>) with the assistance of a trained field enumerator or supervisor of the Taabo HDSS. Questions were either asked in French or translated and explained in any of the local languages (Baoulé, Dioula, or Senoufo). Our questionnaire was carefully developed from previously employed questionnaires in Côte d’Ivoire <xref ref-type="bibr" rid="pone.0064380-Utzinger2">[39]</xref>–<xref ref-type="bibr" rid="pone.0064380-Frst2">[42]</xref>, further adapted during discussions with health personnel and Taabo HDSS staff and pre-tested in a nearby village.</p><p>Additional sociodemographic data on the individual and household level, including information on sex, age, education, main occupation, relationship with the respective head of household, type of housing, and availability of facilities were readily available from the Taabo HDSS database. For further details on the field and laboratory procedures, the reader is referred to Fürst et al. (2012) <xref ref-type="bibr" rid="pone.0064380-Frst1">[31]</xref>. All data from the present study can be obtained from the authors upon request.</p></sec><sec id="s2d"><title>Statistical Analysis</title><p>Data were double-entered and cross-checked in EpiInfo version 3.5.1 (Centers for Disease Control and Prevention; Atlanta, United States of America), and analyzed in STATA version 10.1 (STATA Corp.; College Station, United States of America). Participants for the present study were purposefully sampled, as described in the previous section, with no formal sample size calculation. Only individuals with complete datasets were included in our final analysis.</p><p>Age was stratified into three groups, namely (i) 18–40 years, (ii) 41–60 years, and (iii) >60 years. Educational levels were classified as (i) none, (ii) primary, and (iii) secondary or higher. Occupation was grouped into farmer, fisherman and hunter, housewife, builder and artisan, and being employed in the tertiary sector (includes driver, housekeeper, watchman, merchant, trader, hairdresser, gastronome, healer, nurse, teacher, student, office worker, and policeman). The parasitological results were classified according to infection intensities as expressed in EPG (for <italic>S. mansoni</italic> and soil-transmitted helminths) and eggs/10 ml of urine (for <italic>S. haematobium</italic>), according to World Health Organization (WHO) guidelines <xref ref-type="bibr" rid="pone.0064380-WHO3">[29]</xref>. Furthermore, treatment groups were established, relying on the parasitological results and current WHO recommendations <xref ref-type="bibr" rid="pone.0064380-WHO2">[18]</xref>, i.e., <italic>S. haematobium</italic> and <italic>S. mansoni</italic> infections in the praziquantel treatment group (Tx1), <italic>A. lumbricoides</italic>, <italic>T. trichiura</italic>, and hookworm infections in the benzimidazole treatment group (Tx2), and all single or multiple infections with <italic>S. haematobium</italic>, <italic>S. mansoni</italic>, <italic>A. lumbricoides</italic>, <italic>T. trichiura</italic>, and hookworm in the overarching praziquantel and benzimidazole treatment group (Tx3).</p><p>Initially, χ<sup>2</sup> test statistics and Fisher’s exact test, as appropriate, were used to identify univariable associations between helminth infections and treatment groups, respectively, and reported risk factors, signs, and symptoms. Risk factors, signs, and symptoms significantly associated (p<0.05) were then included as explanatories in a multivariable logistic regression, again with the outcomes helminth infections and treatment groups. A stepwise backward elimination procedure was performed, removing the explanatory variable with the highest p-value one after the other, as long as the Akaike information criterion (AIC) was decreasing. Associations between anamnestic questions and treatment groups were considered as the correct treatment may be more important than exact species identification for control program managers.</p><p>Either each of the remaining and significantly associated (p<0.05) explanatories on their own or all of them combined were used as diagnostic variables to predict helminth infections and treatment group specific classifications of individuals. In case of combining diagnostic variables, a scoring approach was adopted. All significantly associated risk factors, signs, and symptoms were coded 0 or 1 with the higher score indicating elevated odds for being infected with the respective helminth or belonging to a certain treatment group. The scores from all significantly associated risk factors, signs, and symptoms were then summed up to obtain each participant’s combined score. Sensitivity (i.e., proportion of true-positives recognized as positives), specificity (i.e., proportion of true-negatives recognized as negatives), positive predictive value (PPV; i.e., probability that a positively tested individual is a true-positive), and negative predictive value (NPV; i.e., probability that a negatively tested individual is a true-negative) were used to assess the diagnostic performance of each significantly associated risk factor, sign, and symptom on its own and of the combined score at different cut-off levels.</p></sec></sec><sec id="s3"><title>Results</title><sec id="s3a"><title>Study Cohort and Compliance</title><p>Details of our study cohort have been described elsewhere <xref ref-type="bibr" rid="pone.0064380-Frst1">[31]</xref>. Overall, 255 adults were invited (128 males and 127 females; <xref ref-type="fig" rid="pone-0064380-g001">Figure 1</xref>). Sixty individuals were excluded (27 males and 33 females); seven had no written informed consent or failed to have complete questionnaire results, whereas 53 had no valid results from the parasitological examination, mainly because they lacked sufficiently large stool and/or urine samples for diagnostic work-up. Our final study sample consisted of 195 adults (101 males and 94 females) with details of the sociodemographic characteristics summarized in <xref ref-type="table" rid="pone-0064380-t001">Table 1</xref>.</p><fig id="pone-0064380-g001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0064380.g001</object-id><label>Figure 1</label><caption><title>Study participation and compliance.</title><p>The study was carried out in June 2010 in the Taabo health demographic surveillance system, south-central Côte d’Ivoire.</p></caption><graphic xlink:href="pone.0064380.g001"></graphic></fig><table-wrap id="pone-0064380-t001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0064380.t001</object-id><label>Table 1</label><caption><title>Sociodemographic characteristics among the 195 study participants with complete questionnaire and parasitological data.</title></caption><alternatives><graphic id="pone-0064380-t001-1" xlink:href="pone.0064380.t001"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td colspan="2" align="left" rowspan="1">Sociodemographic characteristics</td><td align="left" rowspan="1" colspan="1">Number of people (%)</td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Sex</td><td align="left" rowspan="1" colspan="1">Male</td><td align="left" rowspan="1" colspan="1">101 (51.8)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Female</td><td align="left" rowspan="1" colspan="1">94 (48.2)</td></tr><tr><td align="left" rowspan="1" colspan="1">Age (years)</td><td align="left" rowspan="1" colspan="1">18–40</td><td align="left" rowspan="1" colspan="1">107 (54.9)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">41–60</td><td align="left" rowspan="1" colspan="1">66 (33.9)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>60</td><td align="left" rowspan="1" colspan="1">22 (11.3)</td></tr><tr><td align="left" rowspan="1" colspan="1">Education</td><td align="left" rowspan="1" colspan="1">None</td><td align="left" rowspan="1" colspan="1">106 (54.4)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Primary</td><td align="left" rowspan="1" colspan="1">41 (21.0)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Secondary or higher</td><td align="left" rowspan="1" colspan="1">48 (24.6)</td></tr><tr><td align="left" rowspan="1" colspan="1">Main occupation</td><td align="left" rowspan="1" colspan="1">Farmer</td><td align="left" rowspan="1" colspan="1">123 (63.1)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Tertiary sector<xref ref-type="table-fn" rid="nt102">a</xref></td><td align="left" rowspan="1" colspan="1">49 (25.1)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Housewife</td><td align="left" rowspan="1" colspan="1">13 (6.7)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Builder and artisan</td><td align="left" rowspan="1" colspan="1">8 (4.1)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Fisherman and hunter</td><td align="left" rowspan="1" colspan="1">2 (1.0)</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt101"><p>The study was carried out in June 2010 in the Taabo health demographic surveillance system, south-central Côte d’Ivoire.</p></fn><fn id="nt102"><label>a</label><p>Including driver, housekeeper, watchman, merchant, trader, hairdresser, gastronome, healer, nurse, teacher, student, office worker, and policeman.</p></fn></table-wrap-foot></table-wrap></sec><sec id="s3b"><title>Parasitological Results</title><p><xref ref-type="table" rid="pone-0064380-t002">Table 2</xref> shows the parasitological results. We found very low prevalence for <italic>S. haematobium</italic> and <italic>S. mansoni</italic> (2.1% for each schistosome species). The prevalence for hookworm and <italic>T. trichiura</italic> were 38.5% and 2.6%, whereas no <italic>A. lumbricoides</italic> were found. Most helminth infections were of low intensity. Seven, 76 and 81 participants belonged to Tx1, Tx2, and Tx3, respectively.</p><table-wrap id="pone-0064380-t002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0064380.t002</object-id><label>Table 2</label><caption><title>Prevalence and intensity of helminth infections among the 195 study participants.</title></caption><alternatives><graphic id="pone-0064380-t002-2" xlink:href="pone.0064380.t002"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td align="left" rowspan="1" colspan="1">Parasite</td><td align="left" rowspan="1" colspan="1">Infection intensity<xref ref-type="table-fn" rid="nt105">a</xref></td><td align="left" rowspan="1" colspan="1">Number of people (%)</td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Schistosoma haematobium</italic></td><td align="left" rowspan="1" colspan="1">Negative</td><td align="left" rowspan="1" colspan="1">191 (97.9)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Light</td><td align="left" rowspan="1" colspan="1">4 (2.1)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Heavy</td><td align="left" rowspan="1" colspan="1">0</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Schistosoma mansoni</italic></td><td align="left" rowspan="1" colspan="1">Negative</td><td align="left" rowspan="1" colspan="1">191 (97.9)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Light</td><td align="left" rowspan="1" colspan="1">3 (1.6)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Moderate</td><td align="left" rowspan="1" colspan="1">1 (0.5)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Heavy</td><td align="left" rowspan="1" colspan="1">0</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx1 (<italic>Sh</italic>+<italic>Sm</italic>)</td><td align="left" rowspan="1" colspan="1">Negative</td><td align="left" rowspan="1" colspan="1">188 (96.4)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Positive</td><td align="left" rowspan="1" colspan="1">7 (3.6)</td></tr><tr><td align="left" rowspan="1" colspan="1">Hookworm</td><td align="left" rowspan="1" colspan="1">Negative</td><td align="left" rowspan="1" colspan="1">120 (61.5)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Light</td><td align="left" rowspan="1" colspan="1">72 (36.9)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Moderate</td><td align="left" rowspan="1" colspan="1">3 (1.6)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Heavy</td><td align="left" rowspan="1" colspan="1">0</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Trichuris trichiura</italic></td><td align="left" rowspan="1" colspan="1">Negative</td><td align="left" rowspan="1" colspan="1">190 (97.4)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Light</td><td align="left" rowspan="1" colspan="1">4 (2.1)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Moderate</td><td align="left" rowspan="1" colspan="1">1 (0.5)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Heavy</td><td align="left" rowspan="1" colspan="1">0</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Ascaris lumbricoides</italic></td><td align="left" rowspan="1" colspan="1">Negative</td><td align="left" rowspan="1" colspan="1">195 (100.0)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Light</td><td align="left" rowspan="1" colspan="1">0</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Moderate</td><td align="left" rowspan="1" colspan="1">0</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Heavy</td><td align="left" rowspan="1" colspan="1">0</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx2 (Hw+<italic>Tt</italic>+<italic>Al</italic>)</td><td align="left" rowspan="1" colspan="1">Negative</td><td align="left" rowspan="1" colspan="1">119 (61.0)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Positive</td><td align="left" rowspan="1" colspan="1">76 (39.0)</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx3 (<italic>Sh</italic>+<italic>Sm</italic>+Hw+<italic>Tt</italic>+<italic>Al</italic>)</td><td align="left" rowspan="1" colspan="1">Negative</td><td align="left" rowspan="1" colspan="1">114 (58.5)</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Positive</td><td align="left" rowspan="1" colspan="1">81 (41.5)</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt103"><p>The study was carried out in June 2010 in the Taabo health demographic surveillance system, south-central Côte d’Ivoire.</p></fn><fn id="nt104"><p><italic>Al</italic>, <italic>A. lumbricoides</italic>; Hw, hookworm; <italic>Sh</italic>, <italic>S. haematobium</italic>; <italic>Sm</italic>, <italic>S. mansoni</italic>; <italic>Tt</italic>, <italic>T. trichiura</italic>; Tx1, treatment group 1 (i.e., praziquantel against schistosomiasis); Tx2, treatment group 2 (i.e., benzimidazole against soil-transmitted helminthiasis); Tx3, treatment group 3 (i.e., praziquantel and benzimidazole against schistosomiasis and soil-transmitted helminthiasis, respectively).</p></fn><fn id="nt105"><label>a</label><p>Infection intensities according to WHO guidelines <xref ref-type="bibr" rid="pone.0064380-WHO3">[29]</xref>.</p></fn></table-wrap-foot></table-wrap></sec><sec id="s3c"><title>Results from Univariable Analysis</title><p>Based on univariable χ<sup>2</sup> test statistics and Fisher’s exact test, we identified one risk factor and one symptom that were significantly associated with <italic>S. haematobium</italic> infection, three risk factors that were significantly associated with <italic>S. mansoni</italic>, 18 risk factors that were significantly associated with hookworm infection, and two risk factors that were significantly associated with <italic>T. trichiura</italic> infections (<xref ref-type="table" rid="pone-0064380-t003">Table 3</xref>). Four risk factors and two symptoms were significantly associated with Tx1, 18 risk factors with Tx2, and 11 risk factors with Tx3, as shown in <xref ref-type="table" rid="pone-0064380-t004">Table 4</xref>.</p><table-wrap id="pone-0064380-t003" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0064380.t003</object-id><label>Table 3</label><caption><title>Risk factors, signs, and symptoms significantly (p<0.05) associated with helminth infections, as determined by univariable analysis.</title></caption><alternatives><graphic id="pone-0064380-t003-3" xlink:href="pone.0064380.t003"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td align="left" rowspan="1" colspan="1">Parasite</td><td align="left" rowspan="1" colspan="1">Risk factor, sign, and symptom</td><td align="left" rowspan="1" colspan="1">p-value<xref ref-type="table-fn" rid="nt107">a</xref></td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Schistosoma haematobium</italic></td><td align="left" rowspan="1" colspan="1">Vertigo</td><td align="left" rowspan="1" colspan="1">0.026</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Worm infections considered frequent in household</td><td align="left" rowspan="1" colspan="1">0.027</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Schistosoma mansoni</italic></td><td align="left" rowspan="1" colspan="1">Occupation: farmer</td><td align="left" rowspan="1" colspan="1">0.018</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Occupation: housewife</td><td align="left" rowspan="1" colspan="1">0.023</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: rain</td><td align="left" rowspan="1" colspan="1">0.011</td></tr><tr><td align="left" rowspan="1" colspan="1">Hookworm</td><td align="left" rowspan="1" colspan="1">Occupation: farmer</td><td align="left" rowspan="1" colspan="1">0.022</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Occupation: tertiary sector</td><td align="left" rowspan="1" colspan="1">0.027</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Tile or carpet flooring</td><td align="left" rowspan="1" colspan="1">0.011</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Type of toilet: WC</td><td align="left" rowspan="1" colspan="1">0.048</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Open defecation</td><td align="left" rowspan="1" colspan="1">0.014</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: washing oneself</td><td align="left" rowspan="1" colspan="1">0.010</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: cooking</td><td align="left" rowspan="1" colspan="1">0.014</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: washing children</td><td align="left" rowspan="1" colspan="1">0.033</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: cultivating rice</td><td align="left" rowspan="1" colspan="1">0.019</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: religious worship</td><td align="left" rowspan="1" colspan="1">0.045</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: natural water body</td><td align="left" rowspan="1" colspan="1">0.001</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: rain</td><td align="left" rowspan="1" colspan="1">0.042</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: fountain</td><td align="left" rowspan="1" colspan="1">0.011</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Using soap for washing clothes</td><td align="left" rowspan="1" colspan="1">0.015</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Using soap for washing dishes</td><td align="left" rowspan="1" colspan="1">0.024</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Washing hands after defecation</td><td align="left" rowspan="1" colspan="1">0.003</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Washing hands when returning from work</td><td align="left" rowspan="1" colspan="1">0.004</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Worm infections considered frequent in household</td><td align="left" rowspan="1" colspan="1">0.003</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Trichuris trichiura</italic></td><td align="left" rowspan="1" colspan="1">Using soap for washing oneself</td><td align="left" rowspan="1" colspan="1">0.018</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Having a cat</td><td align="left" rowspan="1" colspan="1">0.048</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt106"><p>The study was carried out in June 2010 in the Taabo health demographic surveillance system, south-central Côte d’Ivoire.</p></fn><fn id="nt107"><label>a</label><p>Univariable analysis, using χ<sup>2</sup> test statistics and Fisher’s exact test, as appropriate.</p></fn></table-wrap-foot></table-wrap><table-wrap id="pone-0064380-t004" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0064380.t004</object-id><label>Table 4</label><caption><title>Risk factors, signs, and symptoms significantly (p<0.05) associated with treatment groups, as determined by univariable analysis.</title></caption><alternatives><graphic id="pone-0064380-t004-4" xlink:href="pone.0064380.t004"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td align="left" rowspan="1" colspan="1">Treatment group<xref ref-type="table-fn" rid="nt109">a</xref></td><td align="left" rowspan="1" colspan="1">Risk factor, sign, and symptom</td><td align="left" rowspan="1" colspan="1">p-value<xref ref-type="table-fn" rid="nt110">b</xref></td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Tx1</td><td align="left" rowspan="1" colspan="1">Occupation: farmer</td><td align="left" rowspan="1" colspan="1">0.011</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Occupation: builder and artisan</td><td align="left" rowspan="1" colspan="1">0.028</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: rain</td><td align="left" rowspan="1" colspan="1">0.005</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Headache</td><td align="left" rowspan="1" colspan="1">0.049</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Vertigo</td><td align="left" rowspan="1" colspan="1">0.018</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Worm infections considered frequent in household</td><td align="left" rowspan="1" colspan="1">0.020</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx2</td><td align="left" rowspan="1" colspan="1">Occupation: farmer</td><td align="left" rowspan="1" colspan="1">0.015</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Occupation: tertiary sector</td><td align="left" rowspan="1" colspan="1">0.018</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Tile or carpet flooring</td><td align="left" rowspan="1" colspan="1">0.010</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Type of toilet: WC</td><td align="left" rowspan="1" colspan="1">0.047</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Open defecation</td><td align="left" rowspan="1" colspan="1">0.022</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: washing oneself</td><td align="left" rowspan="1" colspan="1">0.010</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: cooking</td><td align="left" rowspan="1" colspan="1">0.022</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: washing children</td><td align="left" rowspan="1" colspan="1">0.035</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: cultivating rice</td><td align="left" rowspan="1" colspan="1">0.010</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: natural water body</td><td align="left" rowspan="1" colspan="1">0.001</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: rain</td><td align="left" rowspan="1" colspan="1">0.042</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: fountain</td><td align="left" rowspan="1" colspan="1">0.017</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Using soap for washing clothes</td><td align="left" rowspan="1" colspan="1">0.015</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Using soap for washing dishes</td><td align="left" rowspan="1" colspan="1">0.025</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Washing hands after defecation</td><td align="left" rowspan="1" colspan="1">0.005</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Washing hands when returning from work</td><td align="left" rowspan="1" colspan="1">0.004</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Having poultry</td><td align="left" rowspan="1" colspan="1">0.043</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Worm infections considered frequent in household</td><td align="left" rowspan="1" colspan="1">0.004</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx3</td><td align="left" rowspan="1" colspan="1">Occupation: tertiary sector</td><td align="left" rowspan="1" colspan="1">0.044</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Tile or carpet flooring</td><td align="left" rowspan="1" colspan="1">0.009</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Uncemented latrine</td><td align="left" rowspan="1" colspan="1">0.028</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: washing oneself</td><td align="left" rowspan="1" colspan="1">0.011</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Natural water contact: cultivating rice</td><td align="left" rowspan="1" colspan="1">0.011</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: natural water body</td><td align="left" rowspan="1" colspan="1">0.003</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: fountain</td><td align="left" rowspan="1" colspan="1">0.018</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Using soap for washing clothes</td><td align="left" rowspan="1" colspan="1">0.018</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Washing hands after defecation</td><td align="left" rowspan="1" colspan="1">0.005</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Washing hands when returning from work</td><td align="left" rowspan="1" colspan="1">0.002</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Worm infections considered frequent in household</td><td align="left" rowspan="1" colspan="1">0.002</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt108"><p>The study was carried out in June 2010 in the Taabo health demographic surveillance system, south-central Côte d’Ivoire.</p></fn><fn id="nt109"><label>a</label><p>Tx1, treatment group 1 (i.e., praziquantel against schistosomiasis); Tx2, treatment group 2 (i.e., benzimidazole against soil-transmitted helminthiasis); Tx3, treatment group 3 (i.e., praziquantel and benzimidazole against schistosomiasis and soil-transmitted helminthiasis, respectively).</p></fn><fn id="nt110"><label>b</label><p>Univariable analysis, using χ<sup>2</sup> test statistics and Fisher’s exact test, as appropriate.</p></fn></table-wrap-foot></table-wrap></sec><sec id="s3d"><title>Results from Multivariable Analysis</title><p>Many of the univariably significantly associated risk factors, signs, and symptoms presented in <xref ref-type="table" rid="pone-0064380-t003">Tables 3</xref> and <xref ref-type="table" rid="pone-0064380-t004">4</xref> dropped out when subjecting them to multivariable logistic regression with a stepwise backward elimination procedure, as shown in <xref ref-type="table" rid="pone-0064380-t005">Table 5</xref>. The only remaining significant explanatory variable for <italic>S. mansoni</italic> infection was being a housewife (odds ratio (OR) = 10.3, 95% confidence interval (CI) 1.1–102.1), whereas all explanatories for <italic>S. haematobium</italic> were eliminated. People belonging to the praziquantel treatment group (Tx1) had an increased risk for vertigo (OR = 16.9, 95% CI 1.2–34.6).</p><table-wrap id="pone-0064380-t005" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0064380.t005</object-id><label>Table 5</label><caption><title>Risk factors, signs, and symptoms significantly (p<0.05) associated with parasites and treatment groups, as determined by multivariable logistic regression modeling.</title></caption><alternatives><graphic id="pone-0064380-t005-5" xlink:href="pone.0064380.t005"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td align="left" rowspan="1" colspan="1">Parasite ortreatment group<xref ref-type="table-fn" rid="nt112">a</xref></td><td align="left" rowspan="1" colspan="1">Risk factor, sign, and symptom</td><td align="left" rowspan="1" colspan="1">Adjusted oddsratio [95% CI]<xref ref-type="table-fn" rid="nt113">b</xref></td><td align="left" rowspan="1" colspan="1">Sensitivity (%)[95% CI]<xref ref-type="table-fn" rid="nt113">b</xref></td><td align="left" rowspan="1" colspan="1">Specificity (%)[95% CI]<xref ref-type="table-fn" rid="nt113">b</xref></td><td align="left" rowspan="1" colspan="1">Positivepredictive value [95% CI]<xref ref-type="table-fn" rid="nt113">b</xref></td><td align="left" rowspan="1" colspan="1">Negative predictivevalue [95% CI]<xref ref-type="table-fn" rid="nt113">b</xref></td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>S. mansoni</italic></td><td align="left" rowspan="1" colspan="1">Housewife</td><td align="left" rowspan="1" colspan="1">10.3 [1.1, 102.1]</td><td align="left" rowspan="1" colspan="1">50.0 [43.0, 57.0]</td><td align="left" rowspan="1" colspan="1">94.2 [91.0, 97.5]</td><td align="left" rowspan="1" colspan="1">15.4 [10.3, 20.5]</td><td align="left" rowspan="1" colspan="1">98.9 [97.4, 100.0]</td></tr><tr><td align="left" rowspan="1" colspan="1">Hookworm</td><td align="left" rowspan="1" colspan="1">Natural water contact: cultivating rice</td><td align="left" rowspan="1" colspan="1">2.6 [1.2, 5.7]</td><td align="left" rowspan="1" colspan="1">29.3 [22.9, 35.7]</td><td align="left" rowspan="1" colspan="1">85.0 [80.0, 90.0]</td><td align="left" rowspan="1" colspan="1">55.0 [48.0, 62.0]</td><td align="left" rowspan="1" colspan="1">65.8 [59.2, 72.5]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: fountain</td><td align="left" rowspan="1" colspan="1">0.4 [0.2, 0.8]</td><td align="left" rowspan="1" colspan="1">29.3 [22.9, 35.7]</td><td align="left" rowspan="1" colspan="1">51.7 [44.6, 58.7]</td><td align="left" rowspan="1" colspan="1">27.5 [21.2, 33.8]</td><td align="left" rowspan="1" colspan="1">53.9 [46.9, 60.9]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Using soap for washing clothes</td><td align="left" rowspan="1" colspan="1">0.1 [0.02, 0.6]</td><td align="left" rowspan="1" colspan="1">89.3 [85.0, 93.7]</td><td align="left" rowspan="1" colspan="1">1.7 [0.1, 3.5]</td><td align="left" rowspan="1" colspan="1">36.2 [29.5, 43.0]</td><td align="left" rowspan="1" colspan="1">20.0 [14.4, 25.6]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Worm infections considered frequent in household</td><td align="left" rowspan="1" colspan="1">4.6 [1.4, 14.6]</td><td align="left" rowspan="1" colspan="1">18.7 [13.2, 24.1]</td><td align="left" rowspan="1" colspan="1">95.0 [91.9, 98.1]</td><td align="left" rowspan="1" colspan="1">70.0 [63.6, 76.4]</td><td align="left" rowspan="1" colspan="1">65.1 [58.5, 71.8]</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>T. trichiura</italic></td><td align="left" rowspan="1" colspan="1">Using soap for washing oneself</td><td align="left" rowspan="1" colspan="1">0.05 [0.01, 0.4]</td><td align="left" rowspan="1" colspan="1">60.0 [53.1, 66.9]</td><td align="left" rowspan="1" colspan="1">3.7 [1.0, 6.3]</td><td align="left" rowspan="1" colspan="1">1.6 [0.1, 3.4]</td><td align="left" rowspan="1" colspan="1">77.8 [71.90, 83.6]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Having a cat</td><td align="left" rowspan="1" colspan="1">10.6 [1.3, 85.8]</td><td align="left" rowspan="1" colspan="1">40.0 [33.1, 46.9]</td><td align="left" rowspan="1" colspan="1">93.2 [89.6, 96.7]</td><td align="left" rowspan="1" colspan="1">13.3 [8.6, 18.1]</td><td align="left" rowspan="1" colspan="1">98.3 [96.5, 100.0]</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx1</td><td align="left" rowspan="1" colspan="1">Vertigo</td><td align="left" rowspan="1" colspan="1">16.9 [1.2, 34.6]</td><td align="left" rowspan="1" colspan="1">85.7 [80.8, 90.6]</td><td align="left" rowspan="1" colspan="1">61.2 [54.3, 68.0]</td><td align="left" rowspan="1" colspan="1">7.6 [3.9, 11.3]</td><td align="left" rowspan="1" colspan="1">99.1 [97.8, 100.0]</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx2</td><td align="left" rowspan="1" colspan="1">Natural water contact: cultivating rice</td><td align="left" rowspan="1" colspan="1">2.9 [1.3, 6.4]</td><td align="left" rowspan="1" colspan="1">30.3 [23.8, 36.7]</td><td align="left" rowspan="1" colspan="1">85.7 [80.8, 90.6]</td><td align="left" rowspan="1" colspan="1">57.5 [50.6, 64.4]</td><td align="left" rowspan="1" colspan="1">65.8 [59.2, 72.5]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: fountain</td><td align="left" rowspan="1" colspan="1">0.4 [0.2, 0.9]</td><td align="left" rowspan="1" colspan="1">30.3 [23.8, 36.7]</td><td align="left" rowspan="1" colspan="1">52.1 [45.1, 59.1]</td><td align="left" rowspan="1" colspan="1">28.8 [22.4, 35.1]</td><td align="left" rowspan="1" colspan="1">53.9 [46.9 60.1]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Using soap for washing clothes</td><td align="left" rowspan="1" colspan="1">0.1 [0.02, 0.6]</td><td align="left" rowspan="1" colspan="1">89.5 [85.2, 93.8]</td><td align="left" rowspan="1" colspan="1">1.7 [0.1, 3.5]</td><td align="left" rowspan="1" colspan="1">36.8 [30.0, 43.5]</td><td align="left" rowspan="1" colspan="1">20.0 [14.4, 25.6]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Washing hands when returning from work</td><td align="left" rowspan="1" colspan="1">0.5 [0.2, 0.9]</td><td align="left" rowspan="1" colspan="1">25.0 [18.9, 31.1]</td><td align="left" rowspan="1" colspan="1">53.8 [46.8, 60.8]</td><td align="left" rowspan="1" colspan="1">25.7 [19.5, 31.8]</td><td align="left" rowspan="1" colspan="1">52.9 [45.9, 59.9]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Worm infections considered frequent in household</td><td align="left" rowspan="1" colspan="1">4.4 [1.4, 14.1]</td><td align="left" rowspan="1" colspan="1">18.4 [13.0, 23.9]</td><td align="left" rowspan="1" colspan="1">95.0 [91.9, 98.0]</td><td align="left" rowspan="1" colspan="1">70.0 [63.6, 76.4]</td><td align="left" rowspan="1" colspan="1">64.6 [57.9, 71.3]</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx3</td><td align="left" rowspan="1" colspan="1">Natural water contact: cultivating rice</td><td align="left" rowspan="1" colspan="1">2.8 [1.3, 6.2]</td><td align="left" rowspan="1" colspan="1">29.6 [23.2, 36.0]</td><td align="left" rowspan="1" colspan="1">86.0 [81.1, 90.8]</td><td align="left" rowspan="1" colspan="1">60.0 [53.1, 66.9]</td><td align="left" rowspan="1" colspan="1">63.2 [56.5, 70.0]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Drinking water: fountain</td><td align="left" rowspan="1" colspan="1">0.4 [0.2, 0.8]</td><td align="left" rowspan="1" colspan="1">30.9 [24.4, 37.4]</td><td align="left" rowspan="1" colspan="1">51.8 [44.7, 58.8]</td><td align="left" rowspan="1" colspan="1">31.3 [24.7, 37.8]</td><td align="left" rowspan="1" colspan="1">51.3 [44.3, 58.3]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Using soap for washing clothes</td><td align="left" rowspan="1" colspan="1">0.1 [0.02, 0.8]</td><td align="left" rowspan="1" colspan="1">90.1 [85.9, 94.3]</td><td align="left" rowspan="1" colspan="1">1.8 [0.01, 3.6]</td><td align="left" rowspan="1" colspan="1">39.5 [32.6, 46.3]</td><td align="left" rowspan="1" colspan="1">20.0 [14.4, 25.6]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Washing hands when returning from work</td><td align="left" rowspan="1" colspan="1">0.4 [0.2, 0.8]</td><td align="left" rowspan="1" colspan="1">24.7 [18.6, 30.7]</td><td align="left" rowspan="1" colspan="1">52.6 [45.6, 59.6]</td><td align="left" rowspan="1" colspan="1">27.0 [20.8, 33.3]</td><td align="left" rowspan="1" colspan="1">49.6 [42.6, 56.6]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">Worm infections considered frequent in household</td><td align="left" rowspan="1" colspan="1">5.4 [1.7, 17.9]</td><td align="left" rowspan="1" colspan="1">18.5 [13.1, 24.0]</td><td align="left" rowspan="1" colspan="1">95.6 [92.7, 98.5]</td><td align="left" rowspan="1" colspan="1">75.0 [68.9, 81.1]</td><td align="left" rowspan="1" colspan="1">62.3 [55.5, 69.1]</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt111"><p>The study was carried out in June 2010 in the Taabo health demographic surveillance system, south-central Côte d’Ivoire. Stepwise backward elimination was performed, removing explanatory variables with the highest p-value one at the time, as long as the Akaike information criterion (AIC) decreased. Diagnostic indicators for each risk factor, sign, and symptom are indicated.</p></fn><fn id="nt112"><label>a</label><p>Tx1, treatment group 1 (i.e., praziquantel against schistosomiasis); Tx2, treatment group 2 (i.e., benzimidazole against soil-transmitted helminthiasis); Tx3, treatment group 3 (i.e., praziquantel and benzimidazole against schistosomiasis and soil-transmitted helminthiasis, respectively).</p></fn><fn id="nt113"><label>b</label><p>CI, confidence interval.</p></fn></table-wrap-foot></table-wrap><p>People cultivating rice (OR = 2.6, 95% CI 1.2–5.7) and considering worm infections as something that occurs frequently in their household (OR = 4.6, 95% CI 1.4–14.6) were at a higher risk for hookworm infection. On the other hand, people who use fountains as a primary source of drinking water (OR = 0.4, 95% CI 0.2–0.8) and those who use soap for washing clothes (OR = 0.1, 95% CI 0.02–0.6) had low odds of hookworm infection. Regarding <italic>T. trichiura</italic>, having a cat as domestic animal was associated with a high odds of infection (OR = 10.6, 95% CI 1.3–85.8), whereas using soap for washing oneself showed a low odds of infection (OR = 0.05, 95% CI 0.01–0.4). People cultivating rice (OR = 2.9, 95% CI 1.3–6.4) and considering worm infections as something that occurs frequently in their household (OR = 4.4, 95% CI 1.4–14.1) showed higher odds and people reporting fountains as an important source of drinking water (OR = 0.4, 95% CI 0.2–0.9), using soap for washing clothes (OR = 0.1, 95% CI 0.02–0.6), and washing their hands when returning from work (OR = 0.5, 95% CI 0.2–0.9) had lower odds to belonging to the benzimidazole treatment group (Tx2).</p><p>Cultivating rice (OR = 2.8, 95% CI 1.3–6.2) or considering worm infections as frequent in the household (OR = 5.4, 95% CI 1.7–17.9) were associated with high odds, whereas fountains as an important source of drinking water (OR = 0.4, 95% CI 0.2–0.8), using soap for washing clothes (OR = 0.1, 95% CI 0.02–0.8), or washing hands when returning from work (OR = 0.4, 95% CI 0.2–0.8) were associated with low odds of schistosomiasis and soil-transmitted helminthiasis, and therefore belonging to the treatment group Tx3.</p></sec><sec id="s3e"><title>Diagnostic Properties of Risk Factors, Signs, and Symptoms</title><p>If each of the significant risk factors, signs, and symptoms revealed from the multivariable analysis was considered as diagnostic variable on its own, estimated sensitivity and specificity showed that at least one of the two diagnostic indicators was ≤50% with the exception of vertigo and belonging to Tx1 (sensitivity = 85.7%, 95% CI 80.8–90.6%; specificity = 61.2%, 95% CI 54.3–68.0%) (<xref ref-type="table" rid="pone-0064380-t005">Table 5</xref>). However, when calculating the respective specificity for vertigo as a diagnostic indicator for Tx1, a high number of false-positives was masked by the high number of correctly identified negatives in the studied low schistosomiasis prevalence sample, as revealed by the inferior corresponding PPV of 7.6% (95% CI 3.9–11.3%).</p><p>When considering the combined score, the only combinations that achieved values >50% for sensitivity, specificity, and the predictive values occurred for Tx2 and Tx3 at the cut-off level >1 (<xref ref-type="table" rid="pone-0064380-t006">Table 6</xref>). By increasing the cut-off levels for predicting positive cases, the number of false-positives decreased and the number of false-negatives increased, consequentially leading to lower sensitivities and higher specificities.</p><table-wrap id="pone-0064380-t006" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0064380.t006</object-id><label>Table 6</label><caption><title>Diagnostic properties of a combined score at different cut-off levels in the diagnosis of helminth infections and treatment groups.</title></caption><alternatives><graphic id="pone-0064380-t006-6" xlink:href="pone.0064380.t006"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col><col align="center" span="1"></col></colgroup><thead><tr><td align="left" rowspan="1" colspan="1">Parasite ortreatment group<xref ref-type="table-fn" rid="nt115">a</xref></td><td align="left" rowspan="1" colspan="1">Combined score cut-offlevels for predictingpositive cases</td><td align="left" rowspan="1" colspan="1">Number of predictedpositive cases</td><td align="left" rowspan="1" colspan="1">Sensitivity (%)[95% CI]<xref ref-type="table-fn" rid="nt116">b</xref></td><td align="left" rowspan="1" colspan="1">Specificity (%)[95% CI]<xref ref-type="table-fn" rid="nt116">b</xref></td><td align="left" rowspan="1" colspan="1">Positive predictivevalue[95% CI]<xref ref-type="table-fn" rid="nt116">b</xref></td><td align="left" rowspan="1" colspan="1">Negative predictivevalue [95% CI]<xref ref-type="table-fn" rid="nt116">b</xref></td></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>S. mansoni</italic></td><td align="left" rowspan="1" colspan="1">>0</td><td align="left" rowspan="1" colspan="1">13</td><td align="left" rowspan="1" colspan="1">50.0 [43.0, 57.0]</td><td align="left" rowspan="1" colspan="1">94.2 [91.0, 97.5]</td><td align="left" rowspan="1" colspan="1">15.4 [10.3, 20.5]</td><td align="left" rowspan="1" colspan="1">98.9 [97.4, 100.0]</td></tr><tr><td align="left" rowspan="1" colspan="1">Hookworm</td><td align="left" rowspan="1" colspan="1">>0</td><td align="left" rowspan="1" colspan="1">142</td><td align="left" rowspan="1" colspan="1">86.7 [81.9, 91.4]</td><td align="left" rowspan="1" colspan="1">35.8 [29.1, 42.6]</td><td align="left" rowspan="1" colspan="1">45.8 [38.8, 52.8]</td><td align="left" rowspan="1" colspan="1">81.1 [75.6, 86.6]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>1</td><td align="left" rowspan="1" colspan="1">36</td><td align="left" rowspan="1" colspan="1">33.3 [26.7, 40.0]</td><td align="left" rowspan="1" colspan="1">90.8 [86.8, 94.9]</td><td align="left" rowspan="1" colspan="1">69.4 [63.0, 75.9]</td><td align="left" rowspan="1" colspan="1">68.6 [62.0, 75.1]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>2</td><td align="left" rowspan="1" colspan="1">7</td><td align="left" rowspan="1" colspan="1">9.3 [5.3, 13.4]</td><td align="left" rowspan="1" colspan="1">100.0 [100.0, 100.0]</td><td align="left" rowspan="1" colspan="1">100.0 [100.0, 100.0]</td><td align="left" rowspan="1" colspan="1">63.8 [57.1, 70.6]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>3</td><td align="left" rowspan="1" colspan="1">0</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>T. trichiura</italic></td><td align="left" rowspan="1" colspan="1">>0</td><td align="left" rowspan="1" colspan="1">23</td><td align="left" rowspan="1" colspan="1">60.0 [53.1, 66.9]</td><td align="left" rowspan="1" colspan="1">89.5 [85.2, 93.8]</td><td align="left" rowspan="1" colspan="1">13.0 [8.3, 17.8]</td><td align="left" rowspan="1" colspan="1">98.8 [97.3, 100.0]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>1</td><td align="left" rowspan="1" colspan="1">1</td><td align="left" rowspan="1" colspan="1">20.0 [14.4, 25.6]</td><td align="left" rowspan="1" colspan="1">100.0 [100.0, 100.0]</td><td align="left" rowspan="1" colspan="1">100.0 [100.0, 100.0]</td><td align="left" rowspan="1" colspan="1">97.9 [95.9, 99.9]</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx1</td><td align="left" rowspan="1" colspan="1">>0</td><td align="left" rowspan="1" colspan="1">79</td><td align="left" rowspan="1" colspan="1">85.7 [80.8, 90.6]</td><td align="left" rowspan="1" colspan="1">61.2 [54.3, 68.0]</td><td align="left" rowspan="1" colspan="1">7.6 [3.9, 11.3]</td><td align="left" rowspan="1" colspan="1">99.1 [97.8, 100.0]</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx2</td><td align="left" rowspan="1" colspan="1">>0</td><td align="left" rowspan="1" colspan="1">168</td><td align="left" rowspan="1" colspan="1">97.4 [95.1, 99.6]</td><td align="left" rowspan="1" colspan="1">21.0 [15.3, 26.7]</td><td align="left" rowspan="1" colspan="1">44.1 [37.1, 51.0]</td><td align="left" rowspan="1" colspan="1">92.6 [88.9, 96.3]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>1</td><td align="left" rowspan="1" colspan="1">108</td><td align="left" rowspan="1" colspan="1">75.0 [68.9, 81.1]</td><td align="left" rowspan="1" colspan="1">57.1 [50.2, 64.1]</td><td align="left" rowspan="1" colspan="1">52.8 [45.8, 59.8]</td><td align="left" rowspan="1" colspan="1">78.2 [72.4, 84.0]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>2</td><td align="left" rowspan="1" colspan="1">25</td><td align="left" rowspan="1" colspan="1">25.0 [18.9, 31.1]</td><td align="left" rowspan="1" colspan="1">95.0 [91.9, 98.0]</td><td align="left" rowspan="1" colspan="1">76.0 [70.0, 82.0]</td><td align="left" rowspan="1" colspan="1">66.5 [59.8, 73.1]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>3</td><td align="left" rowspan="1" colspan="1">5</td><td align="left" rowspan="1" colspan="1">6.6 [3.1, 10.1]</td><td align="left" rowspan="1" colspan="1">100.0 [100.0, 100.0]</td><td align="left" rowspan="1" colspan="1">100.0 [100.0, 100.0]</td><td align="left" rowspan="1" colspan="1">62.6 [55.8, 69.4]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>4</td><td align="left" rowspan="1" colspan="1">0</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td></tr><tr><td align="left" rowspan="1" colspan="1">Tx3</td><td align="left" rowspan="1" colspan="1">>0</td><td align="left" rowspan="1" colspan="1">168</td><td align="left" rowspan="1" colspan="1">96.3 [93.7, 99.0]</td><td align="left" rowspan="1" colspan="1">21.1 [15.3, 26.8]</td><td align="left" rowspan="1" colspan="1">46.4 [39.4, 53.4]</td><td align="left" rowspan="1" colspan="1">88.9 [84.5, 93.3]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>1</td><td align="left" rowspan="1" colspan="1">108</td><td align="left" rowspan="1" colspan="1">75.3 [69.3, 81.4]</td><td align="left" rowspan="1" colspan="1">58.8 [51.9, 65.7]</td><td align="left" rowspan="1" colspan="1">56.5 [49.5, 63.4]</td><td align="left" rowspan="1" colspan="1">77.0 [71.1, 82.9]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>2</td><td align="left" rowspan="1" colspan="1">25</td><td align="left" rowspan="1" colspan="1">24.7 [18.6, 30.7]</td><td align="left" rowspan="1" colspan="1">95.6 [92.7, 98.5]</td><td align="left" rowspan="1" colspan="1">80.0 [74.4, 85.6]</td><td align="left" rowspan="1" colspan="1">64.1 [57.4, 70.9]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>3</td><td align="left" rowspan="1" colspan="1">5</td><td align="left" rowspan="1" colspan="1">6.2 [2.8, 9.6]</td><td align="left" rowspan="1" colspan="1">100.0 [100.0, 100.0]</td><td align="left" rowspan="1" colspan="1">100.0 [100.0, 100.0]</td><td align="left" rowspan="1" colspan="1">60.0 [53.1, 66.9]</td></tr><tr><td align="left" rowspan="1" colspan="1"></td><td align="left" rowspan="1" colspan="1">>4</td><td align="left" rowspan="1" colspan="1">0</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td><td align="left" rowspan="1" colspan="1">n.a.</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="nt114"><p>The study was carried out in June 2010 in the Taabo health demographic surveillance system, south-central Côte d’Ivoire. Significance (p<0.05) of associations between risk factors, signs, and symptoms and parasites and treatment groups, respectively, was previously determined with multivariable logistic regression modeling, including a stepwise backward elimination. Participant’s combined score was obtained by coding all significantly associated risk factors, signs, and symptoms as 0 or 1 with the higher score indicating elevated odds for being infected with the respective helminth or belonging to a certain treatment group. The scores from all significantly associated risk factors, signs, and symptoms were then summed up to obtain each participant’s combined score.</p></fn><fn id="nt115"><label>a</label><p>Tx1, treatment group 1 (i.e., praziquantel against schistosomiasis); Tx2, treatment group 2 (i.e., benzimidazole against soil-transmitted helminthiasis); Tx3, treatment group 3 (i.e., praziquantel and benzimidazole against schistosomiasis and soil-transmitted helminthiasis, respectively).</p></fn><fn id="nt116"><label>b</label><p>CI, confidence interval.</p></fn><fn id="nt117"><p>n.a., not applicable as all individuals predicted as negative at the respective cut-off level.</p></fn></table-wrap-foot></table-wrap></sec></sec><sec id="s4"><title>Discussion</title><p>In the present study, we juxtaposed quality-controlled parasitological data pertaining to schistosome and soil-transmitted helminth infections to sociodemographic data and detailed information on risk factors, signs, and symptoms. Data were obtained during a cross-sectional epidemiological survey conducted in mid-2010 in the Taabo HDSS in south-central Côte d’Ivoire. The intention of this study was twofold. First, we wanted to inform the local health authorities in the study area about risk factors, signs, and symptoms associated with schistosomiasis and soil-transmitted helminthiasis. Second, we were interested in the performance of an anamnestic questionnaire to predict helminth infection in an area subjected to preventive chemotherapy and a strengthened health system. Our study area might therefore represent a typical setting of low-endemicity helminthiasis due to sustained control interventions. Disappointingly, not a single risk factor, sign, or symptom, or combinations of them, revealed promising statistical associations and diagnostic properties in this ‘new parasitic landscape’ of control-induced low endemicity.</p><p>Nevertheless, some issues warrant further discussion. First, the sample size of our study was relatively small (n = 195 adults with complete parasitological and questionnaire data) and, with the exception of hookworm infection (39%), helminth prevalences were indeed very low (<5%). The heads of households and, if possible, a second adult household member of the opposite sex were purposefully sampled from over 1,100 participants who were enrolled during the cross-sectional epidemiological survey in the Taabo HDSS. Importantly, the prevalences of helminth infections in the cross-sectional epidemiological survey were similar to those in our study sample. In the overall survey, the prevalence of hookworm, <italic>S. haematobium</italic>, <italic>S. mansoni</italic>, <italic>T. trichiura</italic>, and <italic>A. lumbricoides</italic> were 31.3%, 3.7%, 2.1%, 1.6%, and 0.8%, respectively (E. K. N’Goran and colleagues, unpublished results).</p><p>Second, the low prevalence and intensity of helminth infection are the likely result of recent control efforts (i.e., annual deworming, health education, improved sanitation, and strengthened health system), which turned the previously polyparasitic study area into an area, where mainly hookworm infections remain. Indeed, N’Goran and colleagues reported a <italic>S. haematobium</italic> prevalence among school children in selected villages of 70% and above in the early and mid-1990s, and still in 2001 <xref ref-type="bibr" rid="pone.0064380-NGoran1">[32]</xref>, <xref ref-type="bibr" rid="pone.0064380-NGoran2">[33]</xref>, <xref ref-type="bibr" rid="pone.0064380-NGoran3">[43]</xref>, whereas surveys conducted in 2008 and 2009 prior to Taabo HDSS-related deworming activities and other control interventions revealed hookworm prevalences of 51–89% <xref ref-type="bibr" rid="pone.0064380-Glinz1">[34]</xref>, <xref ref-type="bibr" rid="pone.0064380-Becker1">[35]</xref>. We can expect similar helminth prevalences in other communities where preventive chemotherapy campaigns are underway. The relative importance of hookworm <italic>versus</italic> the roundworm <italic>A. lumbricoides</italic> will likely be higher now as single-dose albendazole has a considerably lower efficacy in eliminating hookworm compared to <italic>A. lumbricoides</italic> infection <xref ref-type="bibr" rid="pone.0064380-Keiser1">[44]</xref>. For example, a recent study in Yunnan province, People’s Republic of China demonstrated a 67.1% prevalence of hookworm prior to single-dose albendazole, and a prevalence of 20.7% 3–4 weeks after treatment. In the same study, the prevalence of <italic>A. lumbricoides</italic> dropped from 95.1% to 3.7% <xref ref-type="bibr" rid="pone.0064380-Steinmann1">[45]</xref>. With the scale up of preventive chemotherapy against helminthiases, it is likely that hookworm (and <italic>T. trichiura</italic>) infections will predominate in areas which were once highly endemic for schistosomiasis and soil-transmitted helminthiasis. Although our study area can now be considered a low endemicity region for schistosomiasis and a moderate endemicity area for soil-transmitted helminthiasis <xref ref-type="bibr" rid="pone.0064380-WHO3">[29]</xref>, it is a consequence of local helminth control efforts, and might therefore differ to other naturally occurring low or moderate endemic settings.</p><p>Third, as efforts are underway to integrate different control programs targeting multiple neglected tropical diseases <xref ref-type="bibr" rid="pone.0064380-Kabatereine1">[46]</xref>, <xref ref-type="bibr" rid="pone.0064380-Kolaczinski1">[47]</xref>, questions arise as to how one might identify treatment-specific groups most efficiently. Hence, we did not only consider helminth-specific groups, but also treatment-specific groups. This is in line with recent WHO policies, which state that “preventive chemotherapy interventions should be conceived as drug-based rather than disease-based: emphasis should be on the best, coordinated use of the available drugs rather than on specific forms of helminthiasis” <xref ref-type="bibr" rid="pone.0064380-WHO2">[18]</xref>. However, due to the low prevalence <italic>Schistosoma</italic> infection, our praziquantel treatment group (Tx1) also became comparatively small and due to the several fold higher prevalence of hookworm infection, our benzimidazole treatment group (Tx2), and our combined treatment group (Tx3) were clearly driven by the hookworm cases. Therefore, it is difficult to draw firm conclusions and further verification is needed to assess whether anamnestic questionnaires targeting at specific treatment groups may constitute a potential way forward in settings with higher levels of helminth co-endemicity. Also of note when further following a treatment group design, there are other intestinal parasites such as <italic>Strongyloides stercoralis</italic><xref ref-type="bibr" rid="pone.0064380-Glinz1">[34]</xref>, <xref ref-type="bibr" rid="pone.0064380-Becker1">[35]</xref>, <xref ref-type="bibr" rid="pone.0064380-Steinmann2">[48]</xref>, <xref ref-type="bibr" rid="pone.0064380-Knopp1">[49]</xref>, which should be included in future considerations, and their rapid assessment and treatment within treatment groups of existing, efficacious, safe, often donated, or low-cost drug combinations <xref ref-type="bibr" rid="pone.0064380-Molyneux1">[50]</xref>, <xref ref-type="bibr" rid="pone.0064380-Olsen1">[51]</xref> may further enhance the usefulness of the approach.</p><p>Fourth, people reporting that worm infections are frequent in their household when directly asked showed higher odds of hookworm infection, and hence they were more likely to belong to two of the treatment groups considered here (Tx2 and Tx3). People may have some knowledge about helminth infections, possibly acquired during previous research and interventions in the region. If this hypothesis could be confirmed in future studies, the local control efforts should implicitly draw on this knowledge <xref ref-type="bibr" rid="pone.0064380-Acka1">[52]</xref>.</p><p>Fifth, we applied comparatively simple statistical models to analyze the data, including univariable and multivariable models and a scoring method, which is similar to approaches frequently applied in other health-related tests <xref ref-type="bibr" rid="pone.0064380-Folstein1">[53]</xref>–<xref ref-type="bibr" rid="pone.0064380-WHO6">[56]</xref>. Interestingly, an approach using a scoring method and associated flexible score thresholds to predict positive cases may allow for the adaptation of the questionnaire’s sensitivity, specificity, and predictive values. However, better methods to elicit potentially useful anamnestic questions and their optimal combination would be needed.</p><p>Sixth, our anamnestic questionnaire did not perform well for predicting schistosomiasis and soil-transmitted helminth infections in the Taabo HDSS area where control interventions against helminthiases, other neglected tropical diseases, and malaria are underway. The lack of a clear and readily assessable epidemiological and clinical footprint in control-induced low-endemicity settings makes it difficult to inform local health workers and village authorities about the disease and to motivate them to keep up the disease control efforts. Such difficulties are inherent to control-induced low-endemicity settings and have also been noted on the “last mile” of dracunculiasis eradication <xref ref-type="bibr" rid="pone.0064380-Cairncross1">[57]</xref>. Adapting a surveillance-response mechanism (i.e., surveillance for detection of new cases, followed by setting-specific health intervention in response to the cases picked up in the surveillance) should be considered as the way forward.</p><p>In conclusion, we recommend further studies in the Taabo HDSS, but also in other areas with currently still higher prevalences and intensities of helminth infections, to shed additional light on the scope and limits of anamnestic questionnaires as monitoring and guidance tool for neglected tropical disease control programs. Certainly, other clinical factors such as physical exam features and rapid diagnostic tests should be further evaluated as well. Likewise, improvements of alternative helminth diagnostics should be kept in mind in order to continuously appraise the optimal combination of the different diagnostic and monitoring tools. For instance, the Schistosomiasis Consortium for Operational Research and Evaluation (SCORE; <ext-link ext-link-type="uri" xlink:href="http://score.uga.edu">http://score.uga.edu</ext-link>) conducted a multi-country study to assess the diagnostic accuracy of a commercially available point-of-care urine circulating cathodic antigen (POC-CCA) assay for the rapid detection of <italic>S. mansoni</italic> infection, which showed that POC-CCA urine tests are more sensitive than routine Kato-Katz thick smears <xref ref-type="bibr" rid="pone.0064380-Colley1">[58]</xref>. Combining features of the different diagnostic and monitoring tools in consideration of the setting-specific levels of endemicity of the different parasites will become more important as prevalence rates and infection intensities decline due to successful morbidity and infection control and such combinations will likely result in the most efficient care at the point-of-contact. Of note, the European research network NIDIAG is currently aiming at the development of improved, simple, integrated, and cost-effective diagnosis and treatment algorithms for clinical syndromes related to neglected tropical diseases (NIDIAG; <ext-link ext-link-type="uri" xlink:href="http://www.nidiag.org">http://www.nidiag.org</ext-link>) and such more comprehensive approaches may also provide the necessary framework for the additional evaluation and potential application of anamnestic questionnaires.</p></sec><sec sec-type="supplementary-material" id="s5"><title>Supporting Information</title><supplementary-material content-type="local-data" id="pone.0064380.s001"><label>Text S1</label><caption><p><bold>Questionnaire for evaluating the health state of individuals in June 2010 in the Taabo health demographic surveillance system, south-central Côte d’Ivoire (in French).</bold></p><p>(DOC)</p></caption><media xlink:href="pone.0064380.s001.doc" mimetype="application" mime-subtype="msword"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material></sec></body><back><ack><p>We thank all the individuals living in the Taabo health demographic surveillance system (HDSS) for their participation in the annual cross-sectional epidemiological survey done in mid-2010, and particularly for participating in the present study. 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