Evaluation of a TaqMan Array Card for Detection of Central Nervous System Infections.
Identifieur interne : 000856 ( Main/Exploration ); précédent : 000855; suivant : 000857Evaluation of a TaqMan Array Card for Detection of Central Nervous System Infections.
Auteurs : Clayton O. Onyango ; Vladimir Loparev [États-Unis] ; Shirley Lidechi [Kenya] ; Vinod Bhullar [États-Unis] ; D Scott Schmid [États-Unis] ; Kay Radford [États-Unis] ; Michael K. Lo [États-Unis] ; Paul Rota [États-Unis] ; Barbara W. Johnson [États-Unis] ; Jorge Munoz [États-Unis] ; Martina Oneko [Kenya] ; Deron Burton [États-Unis] ; Carolyn M. Black [États-Unis] ; John Neatherlin [Kenya] ; Joel M. Montgomery [Kenya] ; Barry Fields [Kenya]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2017.
Abstract
Infections of the central nervous system (CNS) are often acute, with significant morbidity and mortality. Routine diagnosis of such infections is limited in developing countries and requires modern equipment in advanced laboratories that may be unavailable to a number of patients in sub-Saharan Africa. We developed a TaqMan array card (TAC) that detects multiple pathogens simultaneously from cerebrospinal fluid. The 21-pathogen CNS multiple-pathogen TAC (CNS-TAC) assay includes two parasites (Balamuthia mandrillaris and Acanthamoeba), six bacterial pathogens (Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, Mycoplasma pneumoniae, Mycobacterium tuberculosis, and Bartonella), and 13 viruses (parechovirus, dengue virus, Nipah virus, varicella-zoster virus, mumps virus, measles virus, lyssavirus, herpes simplex viruses 1 and 2, Epstein-Barr virus, enterovirus, cytomegalovirus, and chikungunya virus). The card also includes human RNase P as a nucleic acid extraction control and an internal manufacturer control, GAPDH (glyceraldehyde-3-phosphate dehydrogenase). This CNS-TAC assay can test up to eight samples for all 21 agents within 2.5 h following nucleic acid extraction. The assay was validated for linearity, limit of detection, sensitivity, and specificity by using either live viruses (dengue, mumps, and measles viruses) or nucleic acid material (Nipah and chikungunya viruses). Of 120 samples tested by individual real-time PCR, 35 were positive for eight different targets, whereas the CNS-TAC assay detected 37 positive samples across nine different targets. The CNS-TAC assays showed 85.6% sensitivity and 96.7% specificity. Therefore, the CNS-TAC assay may be useful for outbreak investigation and surveillance of suspected neurological disease.
DOI: 10.1128/JCM.02469-16
PubMed: 28404679
Affiliations:
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Onyango</name><affiliation><nlm:affiliation>Division of Global Health Protection, Centers for Disease Control and Prevention, Nairobi, Kenya xwl4@cdc.gov.</nlm:affiliation><wicri:noCountry code="subField">Kenya xwl4@cdc.gov.</wicri:noCountry></affiliation></author><author><name sortKey="Loparev, Vladimir" sort="Loparev, Vladimir" uniqKey="Loparev V" first="Vladimir" last="Loparev">Vladimir Loparev</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Lidechi, Shirley" sort="Lidechi, Shirley" uniqKey="Lidechi S" first="Shirley" last="Lidechi">Shirley Lidechi</name><affiliation wicri:level="1"><nlm:affiliation>Kenya Medical Research Institute, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Kenya Medical Research Institute, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author><author><name sortKey="Bhullar, Vinod" sort="Bhullar, Vinod" uniqKey="Bhullar V" first="Vinod" last="Bhullar">Vinod Bhullar</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Schmid, D Scott" sort="Schmid, D Scott" uniqKey="Schmid D" first="D Scott" last="Schmid">D Scott Schmid</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Radford, Kay" sort="Radford, Kay" uniqKey="Radford K" first="Kay" last="Radford">Kay Radford</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Lo, Michael K" sort="Lo, Michael K" uniqKey="Lo M" first="Michael K" last="Lo">Michael K. Lo</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Rota, Paul" sort="Rota, Paul" uniqKey="Rota P" first="Paul" last="Rota">Paul Rota</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Johnson, Barbara W" sort="Johnson, Barbara W" uniqKey="Johnson B" first="Barbara W" last="Johnson">Barbara W. Johnson</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Munoz, Jorge" sort="Munoz, Jorge" uniqKey="Munoz J" first="Jorge" last="Munoz">Jorge Munoz</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Oneko, Martina" sort="Oneko, Martina" uniqKey="Oneko M" first="Martina" last="Oneko">Martina Oneko</name><affiliation wicri:level="1"><nlm:affiliation>Kenya Medical Research Institute, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Kenya Medical Research Institute, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author><author><name sortKey="Burton, Deron" sort="Burton, Deron" uniqKey="Burton D" first="Deron" last="Burton">Deron Burton</name><affiliation wicri:level="2"><nlm:affiliation>National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Black, Carolyn M" sort="Black, Carolyn M" uniqKey="Black C" first="Carolyn M" last="Black">Carolyn M. Black</name><affiliation wicri:level="2"><nlm:affiliation>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Neatherlin, John" sort="Neatherlin, John" uniqKey="Neatherlin J" first="John" last="Neatherlin">John Neatherlin</name><affiliation wicri:level="1"><nlm:affiliation>Division of Global Health Protection, Centers for Disease Control and Prevention, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Division of Global Health Protection, Centers for Disease Control and Prevention, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author><author><name sortKey="Montgomery, Joel M" sort="Montgomery, Joel M" uniqKey="Montgomery J" first="Joel M" last="Montgomery">Joel M. Montgomery</name><affiliation wicri:level="1"><nlm:affiliation>Division of Global Health Protection, Centers for Disease Control and Prevention, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Division of Global Health Protection, Centers for Disease Control and Prevention, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author><author><name sortKey="Fields, Barry" sort="Fields, Barry" uniqKey="Fields B" first="Barry" last="Fields">Barry Fields</name><affiliation wicri:level="1"><nlm:affiliation>Division of Global Health Protection, Centers for Disease Control and Prevention, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Division of Global Health Protection, Centers for Disease Control and Prevention, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Infections of the central nervous system (CNS) are often acute, with significant morbidity and mortality. Routine diagnosis of such infections is limited in developing countries and requires modern equipment in advanced laboratories that may be unavailable to a number of patients in sub-Saharan Africa. We developed a TaqMan array card (TAC) that detects multiple pathogens simultaneously from cerebrospinal fluid. The 21-pathogen CNS multiple-pathogen TAC (CNS-TAC) assay includes two parasites (Balamuthia mandrillaris and Acanthamoeba), six bacterial pathogens (Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, Mycoplasma pneumoniae, Mycobacterium tuberculosis, and Bartonella), and 13 viruses (parechovirus, dengue virus, Nipah virus, varicella-zoster virus, mumps virus, measles virus, lyssavirus, herpes simplex viruses 1 and 2, Epstein-Barr virus, enterovirus, cytomegalovirus, and chikungunya virus). The card also includes human RNase P as a nucleic acid extraction control and an internal manufacturer control, GAPDH (glyceraldehyde-3-phosphate dehydrogenase). This CNS-TAC assay can test up to eight samples for all 21 agents within 2.5 h following nucleic acid extraction. The assay was validated for linearity, limit of detection, sensitivity, and specificity by using either live viruses (dengue, mumps, and measles viruses) or nucleic acid material (Nipah and chikungunya viruses). Of 120 samples tested by individual real-time PCR, 35 were positive for eight different targets, whereas the CNS-TAC assay detected 37 positive samples across nine different targets. The CNS-TAC assays showed 85.6% sensitivity and 96.7% specificity. Therefore, the CNS-TAC assay may be useful for outbreak investigation and surveillance of suspected neurological disease.</div></front></TEI><affiliations><list><country><li>Kenya</li><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><noCountry><name sortKey="Onyango, Clayton O" sort="Onyango, Clayton O" uniqKey="Onyango C" first="Clayton O" last="Onyango">Clayton O. Onyango</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Loparev, Vladimir" sort="Loparev, Vladimir" uniqKey="Loparev V" first="Vladimir" last="Loparev">Vladimir Loparev</name></region><name sortKey="Bhullar, Vinod" sort="Bhullar, Vinod" uniqKey="Bhullar V" first="Vinod" last="Bhullar">Vinod Bhullar</name><name sortKey="Black, Carolyn M" sort="Black, Carolyn M" uniqKey="Black C" first="Carolyn M" last="Black">Carolyn M. Black</name><name sortKey="Burton, Deron" sort="Burton, Deron" uniqKey="Burton D" first="Deron" last="Burton">Deron Burton</name><name sortKey="Johnson, Barbara W" sort="Johnson, Barbara W" uniqKey="Johnson B" first="Barbara W" last="Johnson">Barbara W. Johnson</name><name sortKey="Lo, Michael K" sort="Lo, Michael K" uniqKey="Lo M" first="Michael K" last="Lo">Michael K. Lo</name><name sortKey="Munoz, Jorge" sort="Munoz, Jorge" uniqKey="Munoz J" first="Jorge" last="Munoz">Jorge Munoz</name><name sortKey="Radford, Kay" sort="Radford, Kay" uniqKey="Radford K" first="Kay" last="Radford">Kay Radford</name><name sortKey="Rota, Paul" sort="Rota, Paul" uniqKey="Rota P" first="Paul" last="Rota">Paul Rota</name><name sortKey="Schmid, D Scott" sort="Schmid, D Scott" uniqKey="Schmid D" first="D Scott" last="Schmid">D Scott Schmid</name></country><country name="Kenya"><noRegion><name sortKey="Lidechi, Shirley" sort="Lidechi, Shirley" uniqKey="Lidechi S" first="Shirley" last="Lidechi">Shirley Lidechi</name></noRegion><name sortKey="Fields, Barry" sort="Fields, Barry" uniqKey="Fields B" first="Barry" last="Fields">Barry Fields</name><name sortKey="Montgomery, Joel M" sort="Montgomery, Joel M" uniqKey="Montgomery J" first="Joel M" last="Montgomery">Joel M. Montgomery</name><name sortKey="Neatherlin, John" sort="Neatherlin, John" uniqKey="Neatherlin J" first="John" last="Neatherlin">John Neatherlin</name><name sortKey="Oneko, Martina" sort="Oneko, Martina" uniqKey="Oneko M" first="Martina" last="Oneko">Martina Oneko</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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