Molecular epidemiology of HIV in Ghana: Dominance of CRF02_AG
Identifieur interne : 003E40 ( Istex/Corpus ); précédent : 003E39; suivant : 003E41Molecular epidemiology of HIV in Ghana: Dominance of CRF02_AG
Auteurs : Lucia Fischetti ; Ohene Opare-Sem ; Daniel Candotti ; Francis Sarkodie ; Helen Lee ; Jean Pierre AllainSource :
- Journal of Medical Virology [ 0146-6615 ] ; 2004-06.
English descriptors
- KwdEn :
- Aids patients, Asymptomatic, Asymptomatic candidate blood donors, Asymptomatic candidate donors, Asymptomatic population, Biosystem, Carr, Clinical aids, Clinical status, Cornelissen, Delaporte, Double infection, Double infections, Dual infections, Epidemiology, Genetic diversity, Genome, Ghana, Heteroduplex mobility assay, Heyndrickx, Human virus type, Hxb2, Hxb2 positions, Inner primers, Ishikawa, Janssens, Kumasi, Mboup, Mccutchan, Molecular data, Molecular distribution, Molecular epidemiology, Molecular groups, Montavon, Nucleic acid, Outer primers, Peeters, Phylogenetic, Phylogenetic analysis, Present study, Primer, Protease gene, Recombinant, Recombinant form, Recombinant forms, Recombinant strains, Recombination, Reference strains, Retrovirus, Sample collection, Second round, Sequencing, Subtype, Subtypes, Total population, Vergne, Viral, West africa, West african countries.
- Teeft :
- Aids patients, Asymptomatic, Asymptomatic candidate blood donors, Asymptomatic candidate donors, Asymptomatic population, Biosystem, Carr, Clinical aids, Clinical status, Cornelissen, Delaporte, Double infection, Double infections, Dual infections, Epidemiology, Genetic diversity, Genome, Ghana, Heteroduplex mobility assay, Heyndrickx, Human virus type, Hxb2, Hxb2 positions, Inner primers, Ishikawa, Janssens, Kumasi, Mboup, Mccutchan, Molecular data, Molecular distribution, Molecular epidemiology, Molecular groups, Montavon, Nucleic acid, Outer primers, Peeters, Phylogenetic, Phylogenetic analysis, Present study, Primer, Protease gene, Recombinant, Recombinant form, Recombinant forms, Recombinant strains, Recombination, Reference strains, Retrovirus, Sample collection, Second round, Sequencing, Subtype, Subtypes, Total population, Vergne, Viral, West africa, West african countries.
Abstract
Recent studies showed the importance of CRF02_AG in West Africa, although the clinical relevance of these recombinant forms of HIV remains unknown. The present study aimed at determining the molecular diversity of HIV in Ghana and investigating the possible epidemiologic advantage of recombinant HIV‐1. Plasma samples collected in 1999–2002 from two populations of HIV infected individuals (144 asymptomatic candidate blood donors and 169 AIDS patients) were studied and 249 of them were molecularly characterised in gag, pol, and env regions. Five molecular groups were identified: strains clustering with CRF02_AG in all regions (147/249 or 59%), recombinant strains clustering with CRF02_AG in one or two regions (50/249 or 20%), other subtypes, pure or recombinant, but not involving CRF02_AG (37/249 or 15%), HIV‐2 (11/249 or 4.5%), and double infections (4/249 or 1.5%). There was no significant difference in the distribution of HIV‐1 recombinant strains according to clinical presentation. No evidence of a significant increase in CRF02_AG prevalence between 1999 and 2002 was found. Irrespective of clinical condition, CRF02_AG is the predominant molecular form of HIV‐1 in Kumasi, Ghana. J. Med. Virol. 73:158–166, 2004. © 2004 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20070
Links to Exploration step
ISTEX:BEDA12C23AA0858C475661CF3A8D230FF53EADD5Le document en format XML
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The present study aimed at determining the molecular diversity of HIV in Ghana and investigating the possible epidemiologic advantage of recombinant HIV‐1. Plasma samples collected in 1999–2002 from two populations of HIV infected individuals (144 asymptomatic candidate blood donors and 169 AIDS patients) were studied and 249 of them were molecularly characterised in gag, pol, and env regions. Five molecular groups were identified: strains clustering with CRF02_AG in all regions (147/249 or 59%), recombinant strains clustering with CRF02_AG in one or two regions (50/249 or 20%), other subtypes, pure or recombinant, but not involving CRF02_AG (37/249 or 15%), HIV‐2 (11/249 or 4.5%), and double infections (4/249 or 1.5%). There was no significant difference in the distribution of HIV‐1 recombinant strains according to clinical presentation. No evidence of a significant increase in CRF02_AG prevalence between 1999 and 2002 was found. Irrespective of clinical condition, CRF02_AG is the predominant molecular form of HIV‐1 in Kumasi, Ghana. J. Med. Virol. 73:158–166, 2004. © 2004 Wiley‐Liss, Inc.</abstract><qualityIndicators><score>7.22</score><pdfVersion>1.3</pdfVersion><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1255</abstractCharCount><pdfWordCount>6410</pdfWordCount><pdfCharCount>39744</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>185</abstractWordCount></qualityIndicators><title>Molecular epidemiology of HIV in Ghana: Dominance of CRF02_AG</title><genre><json:string>article</json:string></genre><host><title>Journal of Medical 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Transfusion Medicine, University of Cambridge, Cambridge, England<address><country key="GB"></country></address></affiliation></author><author xml:id="author-0005" role="corresp"><persName><forename type="first">Jean Pierre</forename><surname>Allain</surname></persName><email>jpa1000@cam.ac.uk</email><affiliation>Department of Haematology, Division of Transfusion Medicine, University of Cambridge, Cambridge, England<address><country key="GB"></country></address></affiliation><affiliation>Department of Haematology, Division of Transfusion Medicine, East Anglia Blood Centre, Long Road, Cambridge CB2 2PT, UK.===</affiliation></author><idno type="istex">BEDA12C23AA0858C475661CF3A8D230FF53EADD5</idno><idno type="ark">ark:/67375/WNG-VB57S33P-S</idno><idno type="DOI">10.1002/jmv.20070</idno><idno type="unit">JMV20070</idno><idno type="toTypesetVersion">file:JMV.JMV20070.pdf</idno></analytic><monogr><title level="j" type="main">Journal of Medical Virology</title><title level="j" 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The present study aimed at determining the molecular diversity of HIV in Ghana and investigating the possible epidemiologic advantage of recombinant HIV‐1. Plasma samples collected in 1999–2002 from two populations of HIV infected individuals (144 asymptomatic candidate blood donors and 169 AIDS patients) were studied and 249 of them were molecularly characterised in gag, pol, and env regions. Five molecular groups were identified: strains clustering with CRF02_AG in all regions (147/249 or 59%), recombinant strains clustering with CRF02_AG in one or two regions (50/249 or 20%), other subtypes, pure or recombinant, but not involving CRF02_AG (37/249 or 15%), HIV‐2 (11/249 or 4.5%), and double infections (4/249 or 1.5%). There was no significant difference in the distribution of HIV‐1 recombinant strains according to clinical presentation. No evidence of a significant increase in CRF02_AG prevalence between 1999 and 2002 was found. Irrespective of clinical condition, CRF02_AG is the predominant molecular form of HIV‐1 in Kumasi, Ghana. J. Med. Virol. 73:158–166, 2004. © 2004 Wiley‐Liss, Inc.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="kwd1">HIV</term><term xml:id="kwd2">epidemiology</term><term xml:id="kwd3">CRF02_AG</term><term xml:id="kwd4">Ghana</term></keywords><keywords rend="articleCategory"><term>Research Article</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/BEDA12C23AA0858C475661CF3A8D230FF53EADD5/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1096-9071</doi><issn type="print">0146-6615</issn><issn type="electronic">1096-9071</issn><idGroup><id type="product" value="JMV"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF MEDICAL VIROLOGY">Journal of Medical Virology</title><title type="short">J. Med. Virol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="20"><doi origin="wiley" registered="yes">10.1002/jmv.v73:2</doi><numberingGroup><numbering type="journalVolume" number="73">73</numbering><numbering type="journalIssue">2</numbering></numberingGroup><coverDate startDate="2004-06">June 2004</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="2" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jmv.20070</doi><idGroup><id type="unit" value="JMV20070"></id></idGroup><countGroup><count type="pageTotal" number="9"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title></titleGroup><copyright ownership="publisher">Copyright © 2004 Wiley‐Liss, Inc.</copyright><eventGroup><event type="manuscriptAccepted" date="2004-02-09"></event><event type="firstOnline" date="2004-04-26"></event><event type="publishedOnlineFinalForm" date="2004-04-26"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.4.7 mode:FullText source:HeaderRef result:HeaderRef" date="2011-02-24"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst">158</numbering><numbering type="pageLast">166</numbering></numberingGroup><correspondenceTo>Department of Haematology, Division of Transfusion Medicine, East Anglia Blood Centre, Long Road, Cambridge CB2 2PT, UK.===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JMV.JMV20070.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="1"></count><count type="tableTotal" number="5"></count><count type="referenceTotal" number="43"></count><count type="wordTotal" number="1584"></count></countGroup><titleGroup><title type="main" xml:lang="en">Molecular epidemiology of HIV in Ghana: Dominance of CRF02_AG</title><title type="short" xml:lang="en">HIV Molecular Epidemiology in Ghana</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Lucia</givenNames><familyName>Fischetti</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Ohene</givenNames><familyName>Opare‐Sem</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Daniel</givenNames><familyName>Candotti</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Francis</givenNames><familyName>Sarkodie</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Helen</givenNames><familyName>Lee</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Jean Pierre</givenNames><familyName>Allain</familyName></personName><contactDetails><email>jpa1000@cam.ac.uk</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="GB" type="organization"><unparsedAffiliation>Department of Haematology, Division of Transfusion Medicine, University of Cambridge, Cambridge, England</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="GH" type="organization"><unparsedAffiliation>Komfo Anokye Teaching Hospital, Kumasi, Ghana</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="GB" type="organization"><unparsedAffiliation>National Blood Service, Cambridge Blood Centre, Cambridge, England</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">HIV</keyword><keyword xml:id="kwd2">epidemiology</keyword><keyword xml:id="kwd3">CRF02_AG</keyword><keyword xml:id="kwd4">Ghana</keyword></keywordGroup><fundingInfo><fundingAgency>Sentinel Bioscience, Inc. (to LF)</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Recent studies showed the importance of CRF02_AG in West Africa, although the clinical relevance of these recombinant forms of HIV remains unknown. The present study aimed at determining the molecular diversity of HIV in Ghana and investigating the possible epidemiologic advantage of recombinant HIV‐1. Plasma samples collected in 1999–2002 from two populations of HIV infected individuals (144 asymptomatic candidate blood donors and 169 AIDS patients) were studied and 249 of them were molecularly characterised in gag, pol, and env regions. Five molecular groups were identified: strains clustering with CRF02_AG in all regions (147/249 or 59%), recombinant strains clustering with CRF02_AG in one or two regions (50/249 or 20%), other subtypes, pure or recombinant, but not involving CRF02_AG (37/249 or 15%), HIV‐2 (11/249 or 4.5%), and double infections (4/249 or 1.5%). There was no significant difference in the distribution of HIV‐1 recombinant strains according to clinical presentation. No evidence of a significant increase in CRF02_AG prevalence between 1999 and 2002 was found. Irrespective of clinical condition, CRF02_AG is the predominant molecular form of HIV‐1 in Kumasi, Ghana. J. Med. Virol. 73:158–166, 2004. © 2004 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Molecular epidemiology of HIV in Ghana: Dominance of CRF02_AG</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>HIV Molecular Epidemiology in Ghana</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Molecular epidemiology of HIV in Ghana: Dominance of CRF02_AG</title></titleInfo><name type="personal"><namePart type="given">Lucia</namePart><namePart type="family">Fischetti</namePart><affiliation>Department of Haematology, Division of Transfusion Medicine, University of Cambridge, Cambridge, England</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ohene</namePart><namePart type="family">Opare‐Sem</namePart><affiliation>Komfo Anokye Teaching Hospital, Kumasi, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Daniel</namePart><namePart type="family">Candotti</namePart><affiliation>National Blood Service, Cambridge Blood Centre, Cambridge, England</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Francis</namePart><namePart type="family">Sarkodie</namePart><affiliation>Komfo Anokye Teaching Hospital, Kumasi, Ghana</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Helen</namePart><namePart type="family">Lee</namePart><affiliation>Department of Haematology, Division of Transfusion Medicine, University of Cambridge, Cambridge, England</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean Pierre</namePart><namePart type="family">Allain</namePart><affiliation>Department of Haematology, Division of Transfusion Medicine, University of Cambridge, Cambridge, England</affiliation><affiliation>E-mail: jpa1000@cam.ac.uk</affiliation><affiliation>Correspondence address: Department of Haematology, Division of Transfusion Medicine, East Anglia Blood Centre, Long Road, Cambridge CB2 2PT, UK.===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2004-06</dateIssued><dateValid encoding="w3cdtf">2004-02-09</dateValid><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">1</extent><extent unit="tables">5</extent><extent unit="references">43</extent><extent unit="words">1584</extent></physicalDescription><abstract lang="en">Recent studies showed the importance of CRF02_AG in West Africa, although the clinical relevance of these recombinant forms of HIV remains unknown. The present study aimed at determining the molecular diversity of HIV in Ghana and investigating the possible epidemiologic advantage of recombinant HIV‐1. Plasma samples collected in 1999–2002 from two populations of HIV infected individuals (144 asymptomatic candidate blood donors and 169 AIDS patients) were studied and 249 of them were molecularly characterised in gag, pol, and env regions. Five molecular groups were identified: strains clustering with CRF02_AG in all regions (147/249 or 59%), recombinant strains clustering with CRF02_AG in one or two regions (50/249 or 20%), other subtypes, pure or recombinant, but not involving CRF02_AG (37/249 or 15%), HIV‐2 (11/249 or 4.5%), and double infections (4/249 or 1.5%). There was no significant difference in the distribution of HIV‐1 recombinant strains according to clinical presentation. No evidence of a significant increase in CRF02_AG prevalence between 1999 and 2002 was found. Irrespective of clinical condition, CRF02_AG is the predominant molecular form of HIV‐1 in Kumasi, Ghana. J. Med. Virol. 73:158–166, 2004. © 2004 Wiley‐Liss, Inc.</abstract><note type="funding">Sentinel Bioscience, Inc. (to LF)</note><subject lang="en"><genre>keywords</genre><topic>HIV</topic><topic>epidemiology</topic><topic>CRF02_AG</topic><topic>Ghana</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Medical Virology</title></titleInfo><titleInfo type="abbreviated"><title>J. Med. Virol.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0146-6615</identifier><identifier type="eISSN">1096-9071</identifier><identifier type="DOI">10.1002/(ISSN)1096-9071</identifier><identifier type="PublisherID">JMV</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>73</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>158</start><end>166</end><total>9</total></extent></part></relatedItem><identifier type="istex">BEDA12C23AA0858C475661CF3A8D230FF53EADD5</identifier><identifier type="ark">ark:/67375/WNG-VB57S33P-S</identifier><identifier type="DOI">10.1002/jmv.20070</identifier><identifier type="ArticleID">JMV20070</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2004 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/BEDA12C23AA0858C475661CF3A8D230FF53EADD5/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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