SidaSubSaharaV1, Istex, Corpus, bibRecord, 002A24

Molecular genotyping of HIV‐1 in 61 patients with AIDS from Lomé, Togo

Identifieur interne : 002A24 ( Istex/Corpus ); précédent : 002A23; suivant : 002A25

Molecular genotyping of HIV‐1 in 61 patients with AIDS from Lomé, Togo

Auteurs : F. J. Davies ; O. D'Almeida ; E. Timmers ; J. D'Almeida ; M. Fasken ; K. Bassabi ; H. Lee ; J. Allain

Source :

Journal of Medical Virology [ 0146-6615 ] ; 1999-01.

RBID : ISTEX:815E874437143CA908E967A65129CEAFA18B7D2A

English descriptors

KwdEn :
- Accession, Accession number, Amplification products, Bangui definition, Boehringer mannheim, Central africa, Central african countries, Central african republic, Chronic diarrhea, Clinical criteria, Common symptoms, Consensus tree, Delaporte, Direct sequencing, Distance matrices, Distinct genotypes, Dual infection, Dual infections, Fransen, Genetic diversity, Genetic variability, Genotype, Gershy damet, Groen, Heyndrickx, Human immunodeficiency virus type, Human retrovirus, Immunodeficiency, Insertion, Janssens, Lome, Nucleotide, Nucleotide position, Nucleotide sequence, Oropharyngeal candidiasis, Osei kwasi, Patient sequences, Peeters, Perkin elmer, Phylogenetic, Phylogenetic analysis, Phylogenetic relationship, Prevalent genotypes, Primer, Reference sequences, Region sequences, Retrovirus, Sequencing, Southern africa, Subtypes, Togo, Togolese patients, Transfusion medicine, Weight loss, West africa, World health organization.
Teeft :
- Accession, Accession number, Amplification products, Bangui definition, Boehringer mannheim, Central africa, Central african countries, Central african republic, Chronic diarrhea, Clinical criteria, Common symptoms, Consensus tree, Delaporte, Direct sequencing, Distance matrices, Distinct genotypes, Dual infection, Dual infections, Fransen, Genetic diversity, Genetic variability, Genotype, Gershy damet, Groen, Heyndrickx, Human immunodeficiency virus type, Human retrovirus, Immunodeficiency, Insertion, Janssens, Lome, Nucleotide, Nucleotide position, Nucleotide sequence, Oropharyngeal candidiasis, Osei kwasi, Patient sequences, Peeters, Perkin elmer, Phylogenetic, Phylogenetic analysis, Phylogenetic relationship, Prevalent genotypes, Primer, Reference sequences, Region sequences, Retrovirus, Sequencing, Southern africa, Subtypes, Togo, Togolese patients, Transfusion medicine, Weight loss, West africa, World health organization.

Abstract

To study the distribution of HIV types and genotypes, in Lomé, Togo, a random population of patients who met the clinical criteria of the Bangui definition of AIDS and were positive with two independent screening assays for antibodies to HIV‐1 group M, HIV‐2, and HIV‐1 group O was selected. HIV RNA from serum samples was reverse‐transcribed and amplified with degenerate primers annealing to conserved regions of the HIV‐1, HIV‐2, and HIV‐O gag gene. Amplicons were directly sequenced using an automated sequencer. A 262–271‐bp (strain‐dependent) fragment of the gag gene from each patient was phylogenetically analyzed and compared to the corresponding gag sequences of published HIV‐1 sequences of known African genotypes. Genotype A was found in 48 of 60 patient amplicons (80%), subdivided into two clusters. Ten patients (16.7%) were HIV‐1 genotype G; one was genotype D and one genotype H. HIV‐1 genotype B was not found. Amplicons from two patients contained sequence ambiguities, requiring cloning and sequencing of the gag insert. One patient (T52) was apparently infected with HIV‐1 genotypes A and G; whereas HIV‐1 from patient T139 was of genotype A, with 2/10 clones having a three‐codon insertion at nucleotide position 1142 of the gag gene. HIV‐1 genotype A is dominant in Togo; genotype G is frequent and genotype B has not been found. J. Med. Virol. 57:25–30, 1999. © 1999 Wiley‐Liss, Inc.

Url:

https://api.istex.fr/document/815E874437143CA908E967A65129CEAFA18B7D2A/fulltext/pdf

DOI: 10.1002/(SICI)1096-9071(199901)57:1<25::AID-JMV4>3.0.CO;2-B

Links to Exploration step

ISTEX:815E874437143CA908E967A65129CEAFA18B7D2A

Le document en format XML

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<front><div type="abstract" xml:lang="en">To study the distribution of HIV types and genotypes, in Lomé, Togo, a random population of patients who met the clinical criteria of the Bangui definition of AIDS and were positive with two independent screening assays for antibodies to HIV‐1 group M, HIV‐2, and HIV‐1 group O was selected. HIV RNA from serum samples was reverse‐transcribed and amplified with degenerate primers annealing to conserved regions of the HIV‐1, HIV‐2, and HIV‐O gag gene. Amplicons were directly sequenced using an automated sequencer. A 262–271‐bp (strain‐dependent) fragment of the gag gene from each patient was phylogenetically analyzed and compared to the corresponding gag sequences of published HIV‐1 sequences of known African genotypes. Genotype A was found in 48 of 60 patient amplicons (80%), subdivided into two clusters. Ten patients (16.7%) were HIV‐1 genotype G; one was genotype D and one genotype H. HIV‐1 genotype B was not found. Amplicons from two patients contained sequence ambiguities, requiring cloning and sequencing of the gag insert. One patient (T52) was apparently infected with HIV‐1 genotypes A and G; whereas HIV‐1 from patient T139 was of genotype A, with 2/10 clones having a three‐codon insertion at nucleotide position 1142 of the gag gene. HIV‐1 genotype A is dominant in Togo; genotype G is frequent and genotype B has not been found. J. Med. Virol. 57:25–30, 1999. © 1999 Wiley‐Liss, Inc.</div>
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Molecular genotyping of HIV‐1 in 61 patients with AIDS from Lomé, Togo

Molecular genotyping of HIV‐1 in 61 patients with AIDS from Lomé, Togo

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