Evaluation of HIV antigen/antibody combination ELISAs for diagnosis of HIV infection in Dar Es Salaam, Tanzania.
Identifieur interne : 000532 ( PubMed/Curation ); précédent : 000531; suivant : 000533Evaluation of HIV antigen/antibody combination ELISAs for diagnosis of HIV infection in Dar Es Salaam, Tanzania.
Auteurs : Loveness John Urio [Tanzanie] ; Mohamed Ally Mohamed [Tanzanie] ; Janneth Mghamba [Tanzanie] ; Ahmed Abade [Tanzanie] ; Said Aboud [Ghana]Source :
- The Pan African medical journal [ 1937-8688 ] ; 2015.
Descripteurs français
- KwdFr :
- Adulte, Algorithmes, Anticorps anti-VIH (sang), Antigènes du VIH (sang), Donneurs de sang, Femelle, Humains, Infections à VIH (diagnostic), Infections à VIH (sang), Infections à VIH (épidémiologie), Jeune adulte, Mâle, Prévalence, Sensibilité et spécificité, Sérodiagnostic du SIDA (), Tanzanie (épidémiologie), Test ELISA (), Études transversales.
- MESH :
- diagnostic : Infections à VIH.
- sang : Anticorps anti-VIH, Antigènes du VIH, Infections à VIH.
- épidémiologie : Infections à VIH, Tanzanie.
- Adulte, Algorithmes, Donneurs de sang, Femelle, Humains, Jeune adulte, Mâle, Prévalence, Sensibilité et spécificité, Sérodiagnostic du SIDA, Test ELISA, Études transversales.
English descriptors
- KwdEn :
- AIDS Serodiagnosis (methods), Adult, Algorithms, Blood Donors, Cross-Sectional Studies, Enzyme-Linked Immunosorbent Assay (methods), Female, HIV Antibodies (blood), HIV Antigens (blood), HIV Infections (blood), HIV Infections (diagnosis), HIV Infections (epidemiology), Humans, Male, Prevalence, Sensitivity and Specificity, Tanzania (epidemiology), Young Adult.
- MESH :
- chemical , blood : HIV Antibodies, HIV Antigens.
- geographic , epidemiology : Tanzania.
- blood : HIV Infections.
- diagnosis : HIV Infections.
- epidemiology : HIV Infections.
- methods : AIDS Serodiagnosis, Enzyme-Linked Immunosorbent Assay.
- Adult, Algorithms, Blood Donors, Cross-Sectional Studies, Female, Humans, Male, Prevalence, Sensitivity and Specificity, Young Adult.
Abstract
The aim of this study was to evaluate the performance of Enzygnost HIV Integral II antigen/antibody combination ELISAs in order to formulate HIV ELISA testing algorithms for the Ministry of Health and Social Welfare, Tanzania.
DOI: 10.11604/pamj.2015.20.196.4934
PubMed: 26113927
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type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AIDS Serodiagnosis (methods)</term><term>Adult</term><term>Algorithms</term><term>Blood Donors</term><term>Cross-Sectional Studies</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Female</term><term>HIV Antibodies (blood)</term><term>HIV Antigens (blood)</term><term>HIV Infections (blood)</term><term>HIV Infections (diagnosis)</term><term>HIV Infections (epidemiology)</term><term>Humans</term><term>Male</term><term>Prevalence</term><term>Sensitivity and Specificity</term><term>Tanzania (epidemiology)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Algorithmes</term><term>Anticorps anti-VIH (sang)</term><term>Antigènes du VIH (sang)</term><term>Donneurs de sang</term><term>Femelle</term><term>Humains</term><term>Infections à VIH (diagnostic)</term><term>Infections à VIH (sang)</term><term>Infections à VIH (épidémiologie)</term><term>Jeune adulte</term><term>Mâle</term><term>Prévalence</term><term>Sensibilité et spécificité</term><term>Sérodiagnostic du SIDA ()</term><term>Tanzanie (épidémiologie)</term><term>Test ELISA ()</term><term>Études transversales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>HIV Antibodies</term><term>HIV Antigens</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Tanzania</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à VIH</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>AIDS Serodiagnosis</term><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps anti-VIH</term><term>Antigènes du VIH</term><term>Infections à VIH</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Infections à VIH</term><term>Tanzanie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Algorithms</term><term>Blood Donors</term><term>Cross-Sectional Studies</term><term>Female</term><term>Humans</term><term>Male</term><term>Prevalence</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Algorithmes</term><term>Donneurs de sang</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Prévalence</term><term>Sensibilité et spécificité</term><term>Sérodiagnostic du SIDA</term><term>Test ELISA</term><term>Études transversales</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to evaluate the performance of Enzygnost HIV Integral II antigen/antibody combination ELISAs in order to formulate HIV ELISA testing algorithms for the Ministry of Health and Social Welfare, Tanzania.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26113927</PMID><DateCreated><Year>2015</Year><Month>06</Month><Day>26</Day></DateCreated><DateCompleted><Year>2016</Year><Month>03</Month><Day>29</Day></DateCompleted><DateRevised><Year>2015</Year><Month>06</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1937-8688</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>The Pan African medical journal</Title><ISOAbbreviation>Pan Afr Med J</ISOAbbreviation></Journal><ArticleTitle>Evaluation of HIV antigen/antibody combination ELISAs for diagnosis of HIV infection in Dar Es Salaam, Tanzania.</ArticleTitle><Pagination><MedlinePgn>196</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.11604/pamj.2015.20.196.4934</ELocationID><Abstract><AbstractText Label="INTRODUCTION" NlmCategory="BACKGROUND">The aim of this study was to evaluate the performance of Enzygnost HIV Integral II antigen/antibody combination ELISAs in order to formulate HIV ELISA testing algorithms for the Ministry of Health and Social Welfare, Tanzania.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">This was a laboratory-based evaluation of Enzygnost HIV Integral II Antibody/ Antigen, Murex HIV antigen/antibody and Vironostika HIV Uniform II antigen/antibody conducted between October 2011 and May 2012.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">A total of 600 blood samples were included in the evaluation. A total of 209/596 (35.1%) serum samples were confirmed HIV positive. Of these, the prevalence of HIV infection was 2.3% (3/130), 2.3% (3/127), 2.2% (3/139) and 100% (200/200) for VCT clients, ANC attendees, blood donors and CTC patients, respectively. Three hundred and eighty seven (64.9%) were HIV negative samples. Sensitivity was 100% (95% CI; 98.3-100%) for all the three HIV ELISAs. The specificity for the Enzygnost HIV Integral II and Murex was 100% (95% CI; 99.1-100%). The final specificity at repeat testing was 99.5% (95% CI; 98.2-99.9%) for Vironostika. Enzygnost HIV Integral II detected HIV infection seven days since first bleed.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Initial testing using either Vironostika or Murex HIV antigen/antibody combination ELISA followed by testing of reactive samples on the Enzygnost HIV Integral II gave a sensitivity and specificity of 100% with reduced window period. Combination of two HIV antigen/antibody combination ELISAs can be used as an alternative confirmatory testing strategy for screening of donated blood at the National and Zonal blood transfusion centres and in lab diagnosis of HIV infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Urio</LastName><ForeName>Loveness John</ForeName><Initials>LJ</Initials><AffiliationInfo><Affiliation>Tanzania Field Epidemiology and Laboratory Training Program, Tanzania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mohamed</LastName><ForeName>Mohamed Ally</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Tanzania Field Epidemiology and Laboratory Training Program, Tanzania ; Ministry of Health and Social Welfare, Dar Es Salaam, Tanzania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mghamba</LastName><ForeName>Janneth</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Tanzania Field Epidemiology and Laboratory Training Program, Tanzania ; Ministry of Health and Social Welfare, Dar Es Salaam, Tanzania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abade</LastName><ForeName>Ahmed</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Tanzania Field Epidemiology and Laboratory Training Program, Tanzania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aboud</LastName><ForeName>Said</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Mirobiology and Immunology, Muhimbili University of Health and Allied Sciences, Ho, Ghana.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>03</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Uganda</Country><MedlineTA>Pan Afr Med J</MedlineTA><NlmUniqueID>101517926</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015483">HIV Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015488">HIV Antigens</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>MMWR Recomm Rep. 1992 Jul 17;41(RR-12):1-9</RefSource><PMID Version="1">1324395</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Acquir Immune Defic Syndr. 1992;5(2):170-6</RefSource><PMID Version="1">1732510</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>AIDS. 1992 Dec;6(12):1548-50</RefSource><PMID Version="1">1492941</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>AIDS. 1993 Jan;7(1):1-13</RefSource><PMID 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Version="1">11526139</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol Methods. 2002 Feb;100(1-2):115-20</RefSource><PMID Version="1">11742658</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2002 Apr;40(4):1420-6</RefSource><PMID Version="1">11923367</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Lancet. 1992 Sep 26;340(8822):770-2</RefSource><PMID Version="1">1356186</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D015492" MajorTopicYN="N">AIDS Serodiagnosis</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000465" MajorTopicYN="N">Algorithms</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001782" MajorTopicYN="N">Blood 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