CD25+ FoxP3+ Memory CD4 T Cells Are Frequent Targets of HIV Infection In Vivo.
Identifieur interne : 000378 ( PubMed/Corpus ); précédent : 000377; suivant : 000379CD25+ FoxP3+ Memory CD4 T Cells Are Frequent Targets of HIV Infection In Vivo.
Auteurs : Mkunde Chachage ; Georgios Pollakis ; Edmund Osei Kuffour ; Kerstin Haase ; Asli Bauer ; Yuka Nadai ; Lilli Podola ; Petra Clowes ; Matthias Schiemann ; Lynette Henkel ; Dieter Hoffmann ; Sarah Joseph ; Sabin Bhuju ; Leonard Maboko ; Fred Stephen Sarfo ; Kirsten Eberhardt ; Michael Hoelscher ; Torsten Feldt ; Elmar Saathoff ; Christof GeldmacherSource :
- Journal of virology [ 1098-5514 ] ; 2016.
English descriptors
- KwdEn :
- CD4-Positive T-Lymphocytes (chemistry), CD4-Positive T-Lymphocytes (virology), DNA, Viral (analysis), DNA, Viral (genetics), Forkhead Transcription Factors (analysis), HIV (classification), HIV (genetics), HIV (isolation & purification), HIV Infections (virology), Humans, Interleukin-2 Receptor alpha Subunit (analysis), Ki-67 Antigen (analysis), Phylogeny, Receptors, CCR5 (analysis), Sequence Analysis, DNA, T-Lymphocyte Subsets (chemistry), T-Lymphocyte Subsets (virology), env Gene Products, Human Immunodeficiency Virus (genetics).
- MESH :
- chemical , analysis : DNA, Viral, Forkhead Transcription Factors, Interleukin-2 Receptor alpha Subunit, Ki-67 Antigen, Receptors, CCR5.
- chemistry : CD4-Positive T-Lymphocytes, T-Lymphocyte Subsets.
- classification : HIV.
- chemical , genetics : DNA, Viral, HIV, env Gene Products, Human Immunodeficiency Virus.
- isolation & purification : HIV.
- virology : CD4-Positive T-Lymphocytes, HIV Infections, T-Lymphocyte Subsets.
- Humans, Phylogeny, Sequence Analysis, DNA.
Abstract
Interleukin 2 (IL-2) signaling through the IL-2 receptor alpha chain (CD25) facilitates HIV replication in vitro and facilitates homeostatic proliferation of CD25(+) FoxP3(+) CD4(+) T cells. CD25(+) FoxP3(+) CD4(+) T cells may therefore constitute a suitable subset for HIV infection and plasma virion production. CD25(+) FoxP3(+) CD4(+) T cell frequencies, absolute numbers, and the expression of CCR5 and cell cycle marker Ki67 were studied in peripheral blood from HIV(+) and HIV(-) study volunteers. Different memory CD4(+) T cell subsets were then sorted for quantification of cell-associated HIV DNA and phylogenetic analyses of the highly variable EnvV1V3 region in comparison to plasma-derived virus sequences. In HIV(+) subjects, 51% (median) of CD25(+) FoxP3(+) CD4(+) T cells expressed the HIV coreceptor CCR5. Very high frequencies of Ki67(+) cells were detected in CD25(+) FoxP3(+) memory CD4(+) T cells (median, 27.6%) in comparison to CD25(-) FoxP3(-) memory CD4(+) T cells (median, 4.1%; P < 0.0001). HIV DNA content was 15-fold higher in CD25(+) FoxP3(+) memory CD4(+) T cells than in CD25(-) FoxP3(-) T cells (P = 0.003). EnvV1V3 sequences derived from CD25(+) FoxP3(+) memory CD4(+) T cells did not preferentially cluster with plasma-derived sequences. Quasi-identical cell-plasma sequence pairs were rare, and their proportion decreased with the estimated HIV infection duration. These data suggest that specific cellular characteristics of CD25(+) FoxP3(+) memory CD4(+) T cells might facilitate efficient HIV infection in vivo and passage of HIV DNA to cell progeny in the absence of active viral replication. The contribution of this cell population to plasma virion production remains unclear.
DOI: 10.1128/JVI.00612-16
PubMed: 27384654
Links to Exploration step
pubmed:27384654Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">CD25+ FoxP3+ Memory CD4 T Cells Are Frequent Targets of HIV Infection In Vivo.</title><author><name sortKey="Chachage, Mkunde" sort="Chachage, Mkunde" uniqKey="Chachage M" first="Mkunde" last="Chachage">Mkunde Chachage</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania mchachage@nimr-mmrc.org geldmacher@lrz.uni-muenchen.de.</nlm:affiliation></affiliation></author><author><name sortKey="Pollakis, Georgios" sort="Pollakis, Georgios" uniqKey="Pollakis G" first="Georgios" last="Pollakis">Georgios Pollakis</name><affiliation><nlm:affiliation>Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Kuffour, Edmund Osei" sort="Kuffour, Edmund Osei" uniqKey="Kuffour E" first="Edmund Osei" last="Kuffour">Edmund Osei Kuffour</name><affiliation><nlm:affiliation>Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital Düsseldorf, Dusseldorf, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Haase, Kerstin" sort="Haase, Kerstin" uniqKey="Haase K" first="Kerstin" last="Haase">Kerstin Haase</name><affiliation><nlm:affiliation>Department of Genome Oriented Bioinformatics, Technische Universität München, Freising, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Bauer, Asli" sort="Bauer, Asli" uniqKey="Bauer A" first="Asli" last="Bauer">Asli Bauer</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Nadai, Yuka" sort="Nadai, Yuka" uniqKey="Nadai Y" first="Yuka" last="Nadai">Yuka Nadai</name><affiliation><nlm:affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Podola, Lilli" sort="Podola, Lilli" uniqKey="Podola L" first="Lilli" last="Podola">Lilli Podola</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Clowes, Petra" sort="Clowes, Petra" uniqKey="Clowes P" first="Petra" last="Clowes">Petra Clowes</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Schiemann, Matthias" sort="Schiemann, Matthias" uniqKey="Schiemann M" first="Matthias" last="Schiemann">Matthias Schiemann</name><affiliation><nlm:affiliation>Institute for Medical Microbiology, Immunology and Hygiene, Helmholtz Center Munich, Technische Universität München, Munich, Germany Clinical Cooperation Groups Antigen-Specific Immunotherapy and Immune-Monitoring, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Henkel, Lynette" sort="Henkel, Lynette" uniqKey="Henkel L" first="Lynette" last="Henkel">Lynette Henkel</name><affiliation><nlm:affiliation>Institute for Medical Microbiology, Immunology and Hygiene, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Hoffmann, Dieter" sort="Hoffmann, Dieter" uniqKey="Hoffmann D" first="Dieter" last="Hoffmann">Dieter Hoffmann</name><affiliation><nlm:affiliation>Institute of Virology, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Joseph, Sarah" sort="Joseph, Sarah" uniqKey="Joseph S" first="Sarah" last="Joseph">Sarah Joseph</name><affiliation><nlm:affiliation>MRC Clinical Trials Unit at UCL, London, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Bhuju, Sabin" sort="Bhuju, Sabin" uniqKey="Bhuju S" first="Sabin" last="Bhuju">Sabin Bhuju</name><affiliation><nlm:affiliation>Genome Analytics, Helmholtz Centre for Infection Research, Braunschweig, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Maboko, Leonard" sort="Maboko, Leonard" uniqKey="Maboko L" first="Leonard" last="Maboko">Leonard Maboko</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania.</nlm:affiliation></affiliation></author><author><name sortKey="Sarfo, Fred Stephen" sort="Sarfo, Fred Stephen" uniqKey="Sarfo F" first="Fred Stephen" last="Sarfo">Fred Stephen Sarfo</name><affiliation><nlm:affiliation>Kwame Nkrumah University of Science & Technology, Kumasi, Ghana.</nlm:affiliation></affiliation></author><author><name sortKey="Eberhardt, Kirsten" sort="Eberhardt, Kirsten" uniqKey="Eberhardt K" first="Kirsten" last="Eberhardt">Kirsten Eberhardt</name><affiliation><nlm:affiliation>Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Hoelscher, Michael" sort="Hoelscher, Michael" uniqKey="Hoelscher M" first="Michael" last="Hoelscher">Michael Hoelscher</name><affiliation><nlm:affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Feldt, Torsten" sort="Feldt, Torsten" uniqKey="Feldt T" first="Torsten" last="Feldt">Torsten Feldt</name><affiliation><nlm:affiliation>Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital Düsseldorf, Dusseldorf, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Saathoff, Elmar" sort="Saathoff, Elmar" uniqKey="Saathoff E" first="Elmar" last="Saathoff">Elmar Saathoff</name><affiliation><nlm:affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Geldmacher, Christof" sort="Geldmacher, Christof" uniqKey="Geldmacher C" first="Christof" last="Geldmacher">Christof Geldmacher</name><affiliation><nlm:affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany mchachage@nimr-mmrc.org geldmacher@lrz.uni-muenchen.de.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27384654</idno><idno type="pmid">27384654</idno><idno type="doi">10.1128/JVI.00612-16</idno><idno type="wicri:Area/PubMed/Corpus">000378</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000378</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">CD25+ FoxP3+ Memory CD4 T Cells Are Frequent Targets of HIV Infection In Vivo.</title><author><name sortKey="Chachage, Mkunde" sort="Chachage, Mkunde" uniqKey="Chachage M" first="Mkunde" last="Chachage">Mkunde Chachage</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania mchachage@nimr-mmrc.org geldmacher@lrz.uni-muenchen.de.</nlm:affiliation></affiliation></author><author><name sortKey="Pollakis, Georgios" sort="Pollakis, Georgios" uniqKey="Pollakis G" first="Georgios" last="Pollakis">Georgios Pollakis</name><affiliation><nlm:affiliation>Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Kuffour, Edmund Osei" sort="Kuffour, Edmund Osei" uniqKey="Kuffour E" first="Edmund Osei" last="Kuffour">Edmund Osei Kuffour</name><affiliation><nlm:affiliation>Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital Düsseldorf, Dusseldorf, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Haase, Kerstin" sort="Haase, Kerstin" uniqKey="Haase K" first="Kerstin" last="Haase">Kerstin Haase</name><affiliation><nlm:affiliation>Department of Genome Oriented Bioinformatics, Technische Universität München, Freising, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Bauer, Asli" sort="Bauer, Asli" uniqKey="Bauer A" first="Asli" last="Bauer">Asli Bauer</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Nadai, Yuka" sort="Nadai, Yuka" uniqKey="Nadai Y" first="Yuka" last="Nadai">Yuka Nadai</name><affiliation><nlm:affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Podola, Lilli" sort="Podola, Lilli" uniqKey="Podola L" first="Lilli" last="Podola">Lilli Podola</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Clowes, Petra" sort="Clowes, Petra" uniqKey="Clowes P" first="Petra" last="Clowes">Petra Clowes</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Schiemann, Matthias" sort="Schiemann, Matthias" uniqKey="Schiemann M" first="Matthias" last="Schiemann">Matthias Schiemann</name><affiliation><nlm:affiliation>Institute for Medical Microbiology, Immunology and Hygiene, Helmholtz Center Munich, Technische Universität München, Munich, Germany Clinical Cooperation Groups Antigen-Specific Immunotherapy and Immune-Monitoring, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Henkel, Lynette" sort="Henkel, Lynette" uniqKey="Henkel L" first="Lynette" last="Henkel">Lynette Henkel</name><affiliation><nlm:affiliation>Institute for Medical Microbiology, Immunology and Hygiene, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Hoffmann, Dieter" sort="Hoffmann, Dieter" uniqKey="Hoffmann D" first="Dieter" last="Hoffmann">Dieter Hoffmann</name><affiliation><nlm:affiliation>Institute of Virology, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Joseph, Sarah" sort="Joseph, Sarah" uniqKey="Joseph S" first="Sarah" last="Joseph">Sarah Joseph</name><affiliation><nlm:affiliation>MRC Clinical Trials Unit at UCL, London, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Bhuju, Sabin" sort="Bhuju, Sabin" uniqKey="Bhuju S" first="Sabin" last="Bhuju">Sabin Bhuju</name><affiliation><nlm:affiliation>Genome Analytics, Helmholtz Centre for Infection Research, Braunschweig, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Maboko, Leonard" sort="Maboko, Leonard" uniqKey="Maboko L" first="Leonard" last="Maboko">Leonard Maboko</name><affiliation><nlm:affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania.</nlm:affiliation></affiliation></author><author><name sortKey="Sarfo, Fred Stephen" sort="Sarfo, Fred Stephen" uniqKey="Sarfo F" first="Fred Stephen" last="Sarfo">Fred Stephen Sarfo</name><affiliation><nlm:affiliation>Kwame Nkrumah University of Science & Technology, Kumasi, Ghana.</nlm:affiliation></affiliation></author><author><name sortKey="Eberhardt, Kirsten" sort="Eberhardt, Kirsten" uniqKey="Eberhardt K" first="Kirsten" last="Eberhardt">Kirsten Eberhardt</name><affiliation><nlm:affiliation>Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Hoelscher, Michael" sort="Hoelscher, Michael" uniqKey="Hoelscher M" first="Michael" last="Hoelscher">Michael Hoelscher</name><affiliation><nlm:affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Feldt, Torsten" sort="Feldt, Torsten" uniqKey="Feldt T" first="Torsten" last="Feldt">Torsten Feldt</name><affiliation><nlm:affiliation>Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital Düsseldorf, Dusseldorf, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Saathoff, Elmar" sort="Saathoff, Elmar" uniqKey="Saathoff E" first="Elmar" last="Saathoff">Elmar Saathoff</name><affiliation><nlm:affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Geldmacher, Christof" sort="Geldmacher, Christof" uniqKey="Geldmacher C" first="Christof" last="Geldmacher">Christof Geldmacher</name><affiliation><nlm:affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany mchachage@nimr-mmrc.org geldmacher@lrz.uni-muenchen.de.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD4-Positive T-Lymphocytes (chemistry)</term><term>CD4-Positive T-Lymphocytes (virology)</term><term>DNA, Viral (analysis)</term><term>DNA, Viral (genetics)</term><term>Forkhead Transcription Factors (analysis)</term><term>HIV (classification)</term><term>HIV (genetics)</term><term>HIV (isolation & purification)</term><term>HIV Infections (virology)</term><term>Humans</term><term>Interleukin-2 Receptor alpha Subunit (analysis)</term><term>Ki-67 Antigen (analysis)</term><term>Phylogeny</term><term>Receptors, CCR5 (analysis)</term><term>Sequence Analysis, DNA</term><term>T-Lymphocyte Subsets (chemistry)</term><term>T-Lymphocyte Subsets (virology)</term><term>env Gene Products, Human Immunodeficiency Virus (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term><term>Forkhead Transcription Factors</term><term>Interleukin-2 Receptor alpha Subunit</term><term>Ki-67 Antigen</term><term>Receptors, CCR5</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term><term>T-Lymphocyte Subsets</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>HIV</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>HIV</term><term>env Gene Products, Human Immunodeficiency Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>HIV</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term><term>HIV Infections</term><term>T-Lymphocyte Subsets</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Interleukin 2 (IL-2) signaling through the IL-2 receptor alpha chain (CD25) facilitates HIV replication in vitro and facilitates homeostatic proliferation of CD25(+) FoxP3(+) CD4(+) T cells. CD25(+) FoxP3(+) CD4(+) T cells may therefore constitute a suitable subset for HIV infection and plasma virion production. CD25(+) FoxP3(+) CD4(+) T cell frequencies, absolute numbers, and the expression of CCR5 and cell cycle marker Ki67 were studied in peripheral blood from HIV(+) and HIV(-) study volunteers. Different memory CD4(+) T cell subsets were then sorted for quantification of cell-associated HIV DNA and phylogenetic analyses of the highly variable EnvV1V3 region in comparison to plasma-derived virus sequences. In HIV(+) subjects, 51% (median) of CD25(+) FoxP3(+) CD4(+) T cells expressed the HIV coreceptor CCR5. Very high frequencies of Ki67(+) cells were detected in CD25(+) FoxP3(+) memory CD4(+) T cells (median, 27.6%) in comparison to CD25(-) FoxP3(-) memory CD4(+) T cells (median, 4.1%; P < 0.0001). HIV DNA content was 15-fold higher in CD25(+) FoxP3(+) memory CD4(+) T cells than in CD25(-) FoxP3(-) T cells (P = 0.003). EnvV1V3 sequences derived from CD25(+) FoxP3(+) memory CD4(+) T cells did not preferentially cluster with plasma-derived sequences. Quasi-identical cell-plasma sequence pairs were rare, and their proportion decreased with the estimated HIV infection duration. These data suggest that specific cellular characteristics of CD25(+) FoxP3(+) memory CD4(+) T cells might facilitate efficient HIV infection in vivo and passage of HIV DNA to cell progeny in the absence of active viral replication. The contribution of this cell population to plasma virion production remains unclear.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27384654</PMID><DateCreated><Year>2016</Year><Month>09</Month><Day>30</Day></DateCreated><DateCompleted><Year>2017</Year><Month>05</Month><Day>10</Day></DateCompleted><DateRevised><Year>2017</Year><Month>05</Month><Day>10</Day></DateRevised><Article PubModel="Electronic-Print"><Journal><ISSN IssnType="Electronic">1098-5514</ISSN><JournalIssue CitedMedium="Internet"><Volume>90</Volume><Issue>20</Issue><PubDate><Year>2016</Year><Month>Oct</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>CD25+ FoxP3+ Memory CD4 T Cells Are Frequent Targets of HIV Infection In Vivo.</ArticleTitle><Pagination><MedlinePgn>8954-67</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JVI.00612-16</ELocationID><Abstract><AbstractText Label="UNLABELLED">Interleukin 2 (IL-2) signaling through the IL-2 receptor alpha chain (CD25) facilitates HIV replication in vitro and facilitates homeostatic proliferation of CD25(+) FoxP3(+) CD4(+) T cells. CD25(+) FoxP3(+) CD4(+) T cells may therefore constitute a suitable subset for HIV infection and plasma virion production. CD25(+) FoxP3(+) CD4(+) T cell frequencies, absolute numbers, and the expression of CCR5 and cell cycle marker Ki67 were studied in peripheral blood from HIV(+) and HIV(-) study volunteers. Different memory CD4(+) T cell subsets were then sorted for quantification of cell-associated HIV DNA and phylogenetic analyses of the highly variable EnvV1V3 region in comparison to plasma-derived virus sequences. In HIV(+) subjects, 51% (median) of CD25(+) FoxP3(+) CD4(+) T cells expressed the HIV coreceptor CCR5. Very high frequencies of Ki67(+) cells were detected in CD25(+) FoxP3(+) memory CD4(+) T cells (median, 27.6%) in comparison to CD25(-) FoxP3(-) memory CD4(+) T cells (median, 4.1%; P < 0.0001). HIV DNA content was 15-fold higher in CD25(+) FoxP3(+) memory CD4(+) T cells than in CD25(-) FoxP3(-) T cells (P = 0.003). EnvV1V3 sequences derived from CD25(+) FoxP3(+) memory CD4(+) T cells did not preferentially cluster with plasma-derived sequences. Quasi-identical cell-plasma sequence pairs were rare, and their proportion decreased with the estimated HIV infection duration. These data suggest that specific cellular characteristics of CD25(+) FoxP3(+) memory CD4(+) T cells might facilitate efficient HIV infection in vivo and passage of HIV DNA to cell progeny in the absence of active viral replication. The contribution of this cell population to plasma virion production remains unclear.</AbstractText><AbstractText Label="IMPORTANCE" NlmCategory="OBJECTIVE">Despite recent advances in the understanding of AIDS virus pathogenesis, which cell subsets support HIV infection and replication in vivo is incompletely understood. In vitro, the IL-2 signaling pathway and IL-2-dependent cell cycle induction are essential for HIV infection of stimulated T cells. CD25(+) FoxP3(+) memory CD4 T cells, often referred to as regulatory CD4 T cells, depend on IL-2 signaling for homeostatic proliferation in vivo Our results show that CD25(+) FoxP3(+) memory CD4(+) T cells often express the HIV coreceptor CCR5, are significantly more proliferative, and contain more HIV DNA than CD25(-) FoxP3(-) memory CD4 T cell subsets. The specific cellular characteristics of CD25(+) FoxP3(+) memory CD4(+) T cells probably facilitate efficient HIV infection in vivo and passage of HIV DNA to cell progeny in the absence of active viral replication. However, the contribution of this cell subset to plasma viremia remains unclear.</AbstractText><CopyrightInformation>Copyright © 2016, American Society for Microbiology. All Rights Reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chachage</LastName><ForeName>Mkunde</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania mchachage@nimr-mmrc.org geldmacher@lrz.uni-muenchen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pollakis</LastName><ForeName>Georgios</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kuffour</LastName><ForeName>Edmund Osei</ForeName><Initials>EO</Initials><AffiliationInfo><Affiliation>Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital Düsseldorf, Dusseldorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haase</LastName><ForeName>Kerstin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Genome Oriented Bioinformatics, Technische Universität München, Freising, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bauer</LastName><ForeName>Asli</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nadai</LastName><ForeName>Yuka</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Podola</LastName><ForeName>Lilli</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Clowes</LastName><ForeName>Petra</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schiemann</LastName><ForeName>Matthias</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute for Medical Microbiology, Immunology and Hygiene, Helmholtz Center Munich, Technische Universität München, Munich, Germany Clinical Cooperation Groups Antigen-Specific Immunotherapy and Immune-Monitoring, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henkel</LastName><ForeName>Lynette</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institute for Medical Microbiology, Immunology and Hygiene, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hoffmann</LastName><ForeName>Dieter</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Institute of Virology, Helmholtz Center Munich, Technische Universität München, Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Joseph</LastName><ForeName>Sarah</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>MRC Clinical Trials Unit at UCL, London, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bhuju</LastName><ForeName>Sabin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Genome Analytics, Helmholtz Centre for Infection Research, Braunschweig, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maboko</LastName><ForeName>Leonard</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>NIMR Mbeya Medical Research Centre, Mbeya, Tanzania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sarfo</LastName><ForeName>Fred Stephen</ForeName><Initials>FS</Initials><AffiliationInfo><Affiliation>Kwame Nkrumah University of Science & Technology, Kumasi, Ghana.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eberhardt</LastName><ForeName>Kirsten</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hoelscher</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feldt</LastName><ForeName>Torsten</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital Düsseldorf, Dusseldorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saathoff</LastName><ForeName>Elmar</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Geldmacher</LastName><ForeName>Christof</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany mchachage@nimr-mmrc.org geldmacher@lrz.uni-muenchen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>09</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Virol</MedlineTA><NlmUniqueID>0113724</NlmUniqueID><ISSNLinking>0022-538X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C584933">CCR5 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Version="1">15753206</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D015496" MajorTopicYN="N">CD4-Positive T-Lymphocytes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051858" MajorTopicYN="N">Forkhead Transcription Factors</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006678" MajorTopicYN="N">HIV</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" 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PubStatus="entrez"><Year>2016</Year><Month>7</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>7</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27384654</ArticleId><ArticleId IdType="pii">JVI.00612-16</ArticleId><ArticleId IdType="doi">10.1128/JVI.00612-16</ArticleId><ArticleId IdType="pmc">PMC5044822</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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