Prevalence of Toxoplasma gondii infection in HIV-infected patients and food animals and direct genotyping of T. gondii isolates, Southern Ghana.
Identifieur interne : 000122 ( PubMed/Corpus ); précédent : 000121; suivant : 000123Prevalence of Toxoplasma gondii infection in HIV-infected patients and food animals and direct genotyping of T. gondii isolates, Southern Ghana.
Auteurs : Faustina Pappoe ; Weisheng Cheng ; Lin Wang ; Yuanling Li ; Dorcas Obiri-Yeboah ; Samuel Victor Nuvor ; Henock Ambachew ; Xiaodong Hu ; Qingli Luo ; Deyong Chu ; Yuanhong Xu ; Jilong ShenSource :
- Parasitology research [ 1432-1955 ] ; 2017.
Abstract
Toxoplasma gondii is of public health and veterinary importance causing severe diseases in immunocompromised individuals including HIV/AIDS patients and in congenital cases and animals. There is limited information on the epidemiology of T. gondii infection in humans, particularly HIV patients and food animals and the parasite genotypes in Ghana. A total of 394 HIV-infected patients from three hospitals were screened for T. gondii anti-IgG and IgM using ELISA. DNAs from blood samples of seropositve participants and 95 brain tissues of food animals were PCR assayed to detect Toxoplasma gra6. DNA positive samples were genotyped using multilocus nested polymerase chain reaction restriction fragment length polymorphism at 10 loci: sag1, alt.sag2, sag3, btub, gra6, l358, c22-8, c29-2, pk1, and apico. The overall seroprevalence was 74.37% (293/394). Toxoplasma DNAs were detected in 3.07% of the seropositive participants and 9.47% of the animals. Six of the human DNA positive samples were partly typed at sag3: 33.33, 50, and 16.67% isolates had type I, II, and III alleles, respectively. All nine isolates from food animals typed at nine loci except apico were atypical: six isolates were identical to ToxoDB #41 and #145, and one was identical to TgCkBrRj2 all identified in Brazil. The genotype of two isolates has not been reported previously and was named as TgCtGh1. T. gondii seroprevalence is high among the HIV-infected individuals with T. gondii circulating in Ghana being genetically diverse.
DOI: 10.1007/s00436-017-5442-x
PubMed: 28434050
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Prevalence of Toxoplasma gondii infection in HIV-infected patients and food animals and direct genotyping of T. gondii isolates, Southern Ghana.</title><author><name sortKey="Pappoe, Faustina" sort="Pappoe, Faustina" uniqKey="Pappoe F" first="Faustina" last="Pappoe">Faustina Pappoe</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cheng, Weisheng" sort="Cheng, Weisheng" uniqKey="Cheng W" first="Weisheng" last="Cheng">Weisheng Cheng</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><affiliation><nlm:affiliation>Clinical Laboratory, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, 230031, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yuanling" sort="Li, Yuanling" uniqKey="Li Y" first="Yuanling" last="Li">Yuanling Li</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Obiri Yeboah, Dorcas" sort="Obiri Yeboah, Dorcas" uniqKey="Obiri Yeboah D" first="Dorcas" last="Obiri-Yeboah">Dorcas Obiri-Yeboah</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</nlm:affiliation></affiliation></author><author><name sortKey="Nuvor, Samuel Victor" sort="Nuvor, Samuel Victor" uniqKey="Nuvor S" first="Samuel Victor" last="Nuvor">Samuel Victor Nuvor</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</nlm:affiliation></affiliation></author><author><name sortKey="Ambachew, Henock" sort="Ambachew, Henock" uniqKey="Ambachew H" first="Henock" last="Ambachew">Henock Ambachew</name><affiliation><nlm:affiliation>Department of Clinical Laboratory Diagnostics, First Affiliated Hospital Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Hu, Xiaodong" sort="Hu, Xiaodong" uniqKey="Hu X" first="Xiaodong" last="Hu">Xiaodong Hu</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Luo, Qingli" sort="Luo, Qingli" uniqKey="Luo Q" first="Qingli" last="Luo">Qingli Luo</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chu, Deyong" sort="Chu, Deyong" uniqKey="Chu D" first="Deyong" last="Chu">Deyong Chu</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Yuanhong" sort="Xu, Yuanhong" uniqKey="Xu Y" first="Yuanhong" last="Xu">Yuanhong Xu</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shen, Jilong" sort="Shen, Jilong" uniqKey="Shen J" first="Jilong" last="Shen">Jilong Shen</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China. Shenjilong53@126.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28434050</idno><idno type="pmid">28434050</idno><idno type="doi">10.1007/s00436-017-5442-x</idno><idno type="wicri:Area/PubMed/Corpus">000122</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000122</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Prevalence of Toxoplasma gondii infection in HIV-infected patients and food animals and direct genotyping of T. gondii isolates, Southern Ghana.</title><author><name sortKey="Pappoe, Faustina" sort="Pappoe, Faustina" uniqKey="Pappoe F" first="Faustina" last="Pappoe">Faustina Pappoe</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cheng, Weisheng" sort="Cheng, Weisheng" uniqKey="Cheng W" first="Weisheng" last="Cheng">Weisheng Cheng</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><affiliation><nlm:affiliation>Clinical Laboratory, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, 230031, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yuanling" sort="Li, Yuanling" uniqKey="Li Y" first="Yuanling" last="Li">Yuanling Li</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Obiri Yeboah, Dorcas" sort="Obiri Yeboah, Dorcas" uniqKey="Obiri Yeboah D" first="Dorcas" last="Obiri-Yeboah">Dorcas Obiri-Yeboah</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</nlm:affiliation></affiliation></author><author><name sortKey="Nuvor, Samuel Victor" sort="Nuvor, Samuel Victor" uniqKey="Nuvor S" first="Samuel Victor" last="Nuvor">Samuel Victor Nuvor</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</nlm:affiliation></affiliation></author><author><name sortKey="Ambachew, Henock" sort="Ambachew, Henock" uniqKey="Ambachew H" first="Henock" last="Ambachew">Henock Ambachew</name><affiliation><nlm:affiliation>Department of Clinical Laboratory Diagnostics, First Affiliated Hospital Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Hu, Xiaodong" sort="Hu, Xiaodong" uniqKey="Hu X" first="Xiaodong" last="Hu">Xiaodong Hu</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Luo, Qingli" sort="Luo, Qingli" uniqKey="Luo Q" first="Qingli" last="Luo">Qingli Luo</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chu, Deyong" sort="Chu, Deyong" uniqKey="Chu D" first="Deyong" last="Chu">Deyong Chu</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Yuanhong" sort="Xu, Yuanhong" uniqKey="Xu Y" first="Yuanhong" last="Xu">Yuanhong Xu</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shen, Jilong" sort="Shen, Jilong" uniqKey="Shen J" first="Jilong" last="Shen">Jilong Shen</name><affiliation><nlm:affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China. Shenjilong53@126.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Parasitology research</title><idno type="eISSN">1432-1955</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Toxoplasma gondii is of public health and veterinary importance causing severe diseases in immunocompromised individuals including HIV/AIDS patients and in congenital cases and animals. There is limited information on the epidemiology of T. gondii infection in humans, particularly HIV patients and food animals and the parasite genotypes in Ghana. A total of 394 HIV-infected patients from three hospitals were screened for T. gondii anti-IgG and IgM using ELISA. DNAs from blood samples of seropositve participants and 95 brain tissues of food animals were PCR assayed to detect Toxoplasma gra6. DNA positive samples were genotyped using multilocus nested polymerase chain reaction restriction fragment length polymorphism at 10 loci: sag1, alt.sag2, sag3, btub, gra6, l358, c22-8, c29-2, pk1, and apico. The overall seroprevalence was 74.37% (293/394). Toxoplasma DNAs were detected in 3.07% of the seropositive participants and 9.47% of the animals. Six of the human DNA positive samples were partly typed at sag3: 33.33, 50, and 16.67% isolates had type I, II, and III alleles, respectively. All nine isolates from food animals typed at nine loci except apico were atypical: six isolates were identical to ToxoDB #41 and #145, and one was identical to TgCkBrRj2 all identified in Brazil. The genotype of two isolates has not been reported previously and was named as TgCtGh1. T. gondii seroprevalence is high among the HIV-infected individuals with T. gondii circulating in Ghana being genetically diverse.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">28434050</PMID><DateCreated><Year>2017</Year><Month>04</Month><Day>23</Day></DateCreated><DateRevised><Year>2017</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1955</ISSN><JournalIssue CitedMedium="Internet"><Volume>116</Volume><Issue>6</Issue><PubDate><Year>2017</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Parasitology research</Title><ISOAbbreviation>Parasitol. Res.</ISOAbbreviation></Journal><ArticleTitle>Prevalence of Toxoplasma gondii infection in HIV-infected patients and food animals and direct genotyping of T. gondii isolates, Southern Ghana.</ArticleTitle><Pagination><MedlinePgn>1675-1685</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00436-017-5442-x</ELocationID><Abstract><AbstractText>Toxoplasma gondii is of public health and veterinary importance causing severe diseases in immunocompromised individuals including HIV/AIDS patients and in congenital cases and animals. There is limited information on the epidemiology of T. gondii infection in humans, particularly HIV patients and food animals and the parasite genotypes in Ghana. A total of 394 HIV-infected patients from three hospitals were screened for T. gondii anti-IgG and IgM using ELISA. DNAs from blood samples of seropositve participants and 95 brain tissues of food animals were PCR assayed to detect Toxoplasma gra6. DNA positive samples were genotyped using multilocus nested polymerase chain reaction restriction fragment length polymorphism at 10 loci: sag1, alt.sag2, sag3, btub, gra6, l358, c22-8, c29-2, pk1, and apico. The overall seroprevalence was 74.37% (293/394). Toxoplasma DNAs were detected in 3.07% of the seropositive participants and 9.47% of the animals. Six of the human DNA positive samples were partly typed at sag3: 33.33, 50, and 16.67% isolates had type I, II, and III alleles, respectively. All nine isolates from food animals typed at nine loci except apico were atypical: six isolates were identical to ToxoDB #41 and #145, and one was identical to TgCkBrRj2 all identified in Brazil. The genotype of two isolates has not been reported previously and was named as TgCtGh1. T. gondii seroprevalence is high among the HIV-infected individuals with T. gondii circulating in Ghana being genetically diverse.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pappoe</LastName><ForeName>Faustina</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Weisheng</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Parasitology, Zhongshan School of Medicine and the Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen University, Guangzhou, Guangdong, 510080, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Lin</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Clinical Laboratory, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, 230031, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yuanling</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Obiri-Yeboah</LastName><ForeName>Dorcas</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nuvor</LastName><ForeName>Samuel Victor</ForeName><Initials>SV</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ambachew</LastName><ForeName>Henock</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory Diagnostics, First Affiliated Hospital Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Medicine and Health Sciences, Hawassa University, Hawassa, Ethiopia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Xiaodong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Qingli</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chu</LastName><ForeName>Deyong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Yuanhong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Clinical Laboratory Diagnostics, First Affiliated Hospital Anhui Medical University, Hefei, Anhui, 230032, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Jilong</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Immunology and Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, Anhui, 230032, China. Shenjilong53@126.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Clinical Laboratory Diagnostics, First Affiliated Hospital Anhui Medical University, Hefei, Anhui, 230032, China. Shenjilong53@126.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>04</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Parasitol Res</MedlineTA><NlmUniqueID>8703571</NlmUniqueID><ISSNLinking>0932-0113</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Animals</Keyword><Keyword MajorTopicYN="N">Genotyping</Keyword><Keyword MajorTopicYN="N">Ghana</Keyword><Keyword MajorTopicYN="N">HIV</Keyword><Keyword MajorTopicYN="N">Human</Keyword><Keyword MajorTopicYN="N">Prevalence</Keyword><Keyword MajorTopicYN="N">Toxoplasma</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>02</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>04</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>4</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>4</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>4</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28434050</ArticleId><ArticleId IdType="doi">10.1007/s00436-017-5442-x</ArticleId><ArticleId IdType="pii">10.1007/s00436-017-5442-x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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