Field evaluation of alternative HIV testing strategy with a rapid immunobinding assay and an agglutination assay.
Identifieur interne : 000860 ( PubMed/Checkpoint ); précédent : 000859; suivant : 000861Field evaluation of alternative HIV testing strategy with a rapid immunobinding assay and an agglutination assay.
Auteurs : S W Mitchell [États-Unis] ; S. Mboup ; J. Mingle ; D. Sambe ; P. Tukei ; K. Milenge ; J. Nyamongo ; O K Mubarak ; J L Sankale ; D S HansonSource :
- Lancet (London, England) [ 0140-6736 ] ; 1991.
Descripteurs français
- KwdFr :
- Afrique, Anticorps anti-VIH (sang), Faux négatifs, Faux positifs, Humains, Immunotransfert, Sensibilité et spécificité, Séropositivité VIH (diagnostic), Technique de Western, Test ELISA, Tests d'agglutination, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie), VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie), Études d'évaluation comme sujet.
- MESH :
- diagnostic : Séropositivité VIH.
- immunologie : VIH-1 (Virus de l'Immunodéficience Humaine de type 1), VIH-2 (Virus de l'Immunodéficience Humaine de type 2).
- sang : Anticorps anti-VIH.
- Afrique, Faux négatifs, Faux positifs, Humains, Immunotransfert, Sensibilité et spécificité, Technique de Western, Test ELISA, Tests d'agglutination, Études d'évaluation comme sujet.
English descriptors
- KwdEn :
- Africa, Agglutination Tests, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, False Negative Reactions, False Positive Reactions, HIV Antibodies (blood), HIV Seropositivity (diagnosis), HIV-1 (immunology), HIV-2 (immunology), Humans, Immunoblotting, Sensitivity and Specificity.
- MESH :
- chemical , blood : HIV Antibodies.
- geographic : Africa.
- diagnosis : HIV Seropositivity.
- immunology : HIV-1, HIV-2.
- Agglutination Tests, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, False Negative Reactions, False Positive Reactions, Humans, Immunoblotting, Sensitivity and Specificity.
Abstract
A rapid immunobinding assay ('HIVCHEK', Ortho) and an agglutination assay ('Serodia-HIV', Fujirebio) were evaluated as an alternative to enzyme-linked immunosorbent assay (ELISA) and western blot under field conditions in Africa for detection of antibody to human immunodeficiency virus (HIV). 7106 specimens were tested at 25 laboratories in Kenya, Ghana, Senegal, and Zaire. HIVCHEK was used as a screening test, and serodia-HIV as a supplemental test to evaluate these assays in an alternative testing strategy to the standard ELISA/western blot testing procedure. In each country, HIVCHEK was more sensitive and specific than ELISA when compared with western blot. The sensitivity of HIVCHEK ranged from 87.0 to 96.3% and the specificity from 99.0 to 100%. The sensitivity and specificity of serodia-HIV ranged from 85 to 98% and from 88 to 98%, respectively. The sensitivity and specificity were affected by the presence of HIV-2 in Ghana and Senegal. Overall, with an HIV-1 prevalence of 14.8% in Kenya and 22.5% in Zaire, the sensitivities of the alternative strategy were 96.4% and 91.4%, the specificities 99.6% and 100%, the positive predictive values 97.6% and 100%, and the negative predictive values 99.3% and 97.9% for Kenya and Zaire, respectively. With this testing format there was an estimated average cost saving of up to 82% over the conventional strategy with ELISA/western blot. This procedure constitutes a reasonable alternative to the standard ELISA/western blot combination.
PubMed: 1674306
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Nyamongo</name></author><author><name sortKey="Mubarak, O K" sort="Mubarak, O K" uniqKey="Mubarak O" first="O K" last="Mubarak">O K Mubarak</name></author><author><name sortKey="Sankale, J L" sort="Sankale, J L" uniqKey="Sankale J" first="J L" last="Sankale">J L Sankale</name></author><author><name sortKey="Hanson, D S" sort="Hanson, D S" uniqKey="Hanson D" first="D S" last="Hanson">D S Hanson</name></author></analytic><series><title level="j">Lancet (London, England)</title><idno type="ISSN">0140-6736</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Africa</term><term>Agglutination Tests</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Evaluation Studies as Topic</term><term>False Negative Reactions</term><term>False Positive Reactions</term><term>HIV Antibodies (blood)</term><term>HIV Seropositivity (diagnosis)</term><term>HIV-1 (immunology)</term><term>HIV-2 (immunology)</term><term>Humans</term><term>Immunoblotting</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Afrique</term><term>Anticorps anti-VIH (sang)</term><term>Faux négatifs</term><term>Faux positifs</term><term>Humains</term><term>Immunotransfert</term><term>Sensibilité et spécificité</term><term>Séropositivité VIH (diagnostic)</term><term>Technique de Western</term><term>Test ELISA</term><term>Tests d'agglutination</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie)</term><term>Études d'évaluation comme sujet</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>HIV Antibodies</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Africa</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>HIV Seropositivity</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Séropositivité VIH</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2)</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>HIV-1</term><term>HIV-2</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps anti-VIH</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Agglutination Tests</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Evaluation Studies as Topic</term><term>False Negative Reactions</term><term>False Positive Reactions</term><term>Humans</term><term>Immunoblotting</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Afrique</term><term>Faux négatifs</term><term>Faux positifs</term><term>Humains</term><term>Immunotransfert</term><term>Sensibilité et spécificité</term><term>Technique de Western</term><term>Test ELISA</term><term>Tests d'agglutination</term><term>Études d'évaluation comme sujet</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A rapid immunobinding assay ('HIVCHEK', Ortho) and an agglutination assay ('Serodia-HIV', Fujirebio) were evaluated as an alternative to enzyme-linked immunosorbent assay (ELISA) and western blot under field conditions in Africa for detection of antibody to human immunodeficiency virus (HIV). 7106 specimens were tested at 25 laboratories in Kenya, Ghana, Senegal, and Zaire. HIVCHEK was used as a screening test, and serodia-HIV as a supplemental test to evaluate these assays in an alternative testing strategy to the standard ELISA/western blot testing procedure. In each country, HIVCHEK was more sensitive and specific than ELISA when compared with western blot. The sensitivity of HIVCHEK ranged from 87.0 to 96.3% and the specificity from 99.0 to 100%. The sensitivity and specificity of serodia-HIV ranged from 85 to 98% and from 88 to 98%, respectively. The sensitivity and specificity were affected by the presence of HIV-2 in Ghana and Senegal. Overall, with an HIV-1 prevalence of 14.8% in Kenya and 22.5% in Zaire, the sensitivities of the alternative strategy were 96.4% and 91.4%, the specificities 99.6% and 100%, the positive predictive values 97.6% and 100%, and the negative predictive values 99.3% and 97.9% for Kenya and Zaire, respectively. With this testing format there was an estimated average cost saving of up to 82% over the conventional strategy with ELISA/western blot. This procedure constitutes a reasonable alternative to the standard ELISA/western blot combination.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1674306</PMID><DateCreated><Year>1991</Year><Month>06</Month><Day>20</Day></DateCreated><DateCompleted><Year>1991</Year><Month>06</Month><Day>20</Day></DateCompleted><DateRevised><Year>2015</Year><Month>06</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0140-6736</ISSN><JournalIssue CitedMedium="Print"><Volume>337</Volume><Issue>8753</Issue><PubDate><Year>1991</Year><Month>Jun</Month><Day>01</Day></PubDate></JournalIssue><Title>Lancet (London, England)</Title><ISOAbbreviation>Lancet</ISOAbbreviation></Journal><ArticleTitle>Field evaluation of alternative HIV testing strategy with a rapid immunobinding assay and an agglutination assay.</ArticleTitle><Pagination><MedlinePgn>1328-31</MedlinePgn></Pagination><Abstract><AbstractText>A rapid immunobinding assay ('HIVCHEK', Ortho) and an agglutination assay ('Serodia-HIV', Fujirebio) were evaluated as an alternative to enzyme-linked immunosorbent assay (ELISA) and western blot under field conditions in Africa for detection of antibody to human immunodeficiency virus (HIV). 7106 specimens were tested at 25 laboratories in Kenya, Ghana, Senegal, and Zaire. HIVCHEK was used as a screening test, and serodia-HIV as a supplemental test to evaluate these assays in an alternative testing strategy to the standard ELISA/western blot testing procedure. In each country, HIVCHEK was more sensitive and specific than ELISA when compared with western blot. The sensitivity of HIVCHEK ranged from 87.0 to 96.3% and the specificity from 99.0 to 100%. The sensitivity and specificity of serodia-HIV ranged from 85 to 98% and from 88 to 98%, respectively. The sensitivity and specificity were affected by the presence of HIV-2 in Ghana and Senegal. Overall, with an HIV-1 prevalence of 14.8% in Kenya and 22.5% in Zaire, the sensitivities of the alternative strategy were 96.4% and 91.4%, the specificities 99.6% and 100%, the positive predictive values 97.6% and 100%, and the negative predictive values 99.3% and 97.9% for Kenya and Zaire, respectively. With this testing format there was an estimated average cost saving of up to 82% over the conventional strategy with ELISA/western blot. This procedure constitutes a reasonable alternative to the standard ELISA/western blot combination.</AbstractText></Abstract><AuthorList CompleteYN="N"><Author ValidYN="Y"><LastName>Mitchell</LastName><ForeName>S W</ForeName><Initials>SW</Initials><AffiliationInfo><Affiliation>Family Health International, Research Triangle Park Branch, Durham, North Carolina 27709.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mboup</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Mingle</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Sambe</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Tukei</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Milenge</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Nyamongo</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Mubarak</LastName><ForeName>O K</ForeName><Initials>OK</Initials></Author><Author ValidYN="Y"><LastName>Sankale</LastName><ForeName>J L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Hanson</LastName><ForeName>D S</ForeName><Initials>DS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Lancet</MedlineTA><NlmUniqueID>2985213R</NlmUniqueID><ISSNLinking>0140-6736</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015483">HIV Antibodies</NameOfSubstance></Chemical></ChemicalList><CitationSubset>AIM</CitationSubset><CitationSubset>IM</CitationSubset><CitationSubset>X</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000349" MajorTopicYN="N" Type="Geographic">Africa</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000372" MajorTopicYN="Y">Agglutination Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005069" MajorTopicYN="N">Evaluation Studies as Topic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005188" MajorTopicYN="N">False Negative Reactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005189" MajorTopicYN="N">False Positive Reactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015483" MajorTopicYN="N">HIV Antibodies</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006679" MajorTopicYN="N">HIV Seropositivity</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015497" MajorTopicYN="N">HIV-1</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015498" MajorTopicYN="N">HIV-2</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015151" MajorTopicYN="Y">Immunoblotting</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1991</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1991</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1991</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1674306</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><noCountry><name sortKey="Hanson, D S" sort="Hanson, D S" uniqKey="Hanson D" first="D S" last="Hanson">D S Hanson</name><name sortKey="Mboup, S" sort="Mboup, S" uniqKey="Mboup S" first="S" last="Mboup">S. Mboup</name><name sortKey="Milenge, K" sort="Milenge, K" uniqKey="Milenge K" first="K" last="Milenge">K. Milenge</name><name sortKey="Mingle, J" sort="Mingle, J" uniqKey="Mingle J" first="J" last="Mingle">J. Mingle</name><name sortKey="Mubarak, O K" sort="Mubarak, O K" uniqKey="Mubarak O" first="O K" last="Mubarak">O K Mubarak</name><name sortKey="Nyamongo, J" sort="Nyamongo, J" uniqKey="Nyamongo J" first="J" last="Nyamongo">J. Nyamongo</name><name sortKey="Sambe, D" sort="Sambe, D" uniqKey="Sambe D" first="D" last="Sambe">D. Sambe</name><name sortKey="Sankale, J L" sort="Sankale, J L" uniqKey="Sankale J" first="J L" last="Sankale">J L Sankale</name><name sortKey="Tukei, P" sort="Tukei, P" uniqKey="Tukei P" first="P" last="Tukei">P. Tukei</name></noCountry><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Mitchell, S W" sort="Mitchell, S W" uniqKey="Mitchell S" first="S W" last="Mitchell">S W Mitchell</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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